Performance characteristics of a commercially prepared biphasic blood culture bottle.

abstract：:Enterocytozoon bieneusi, a microsporidian parasite, has been recognized since 1985 as an agent of intestinal microsporidiosis leading to malabsorption syndrome, diarrhea, and weight loss in AIDS patients. Recently, however, we have identified E. bieneusi spores in the sputum, bronchoalveolar lavage, and stool samples ...

journal_title：Journal of clinical microbiology

authors： del Aguila C,Lopez-Velez R,Fenoy S,Turrientes C,Cobo J,Navajas R,Visvesvara GS,Croppo GP,Da Silva AJ,Pieniazek NJ

更新日期：1997-07-01 00:00:00

abstract：:The human cytomegalovirus (HCMV) a-sequence (a-seq) is located in the joining region between the long (L) and short (S) unique sequences of the virus (L-S junction), and this hypervariable junction has been used to differentiate HCMV strains. The purpose of this study was to investigate whether there are differences a...

journal_title：Journal of clinical microbiology

authors： Zaia JA,Gallez-Hawkins G,Churchill MA,Morton-Blackshere A,Pande H,Adler SP,Schmidt GM,Forman SJ

更新日期：1990-12-01 00:00:00

abstract：:To study the population structure of Enterococcus faecalis from Polish hospitals, 291 isolates were typed by pulsed-field gel electrophoresis and a novel multilocus sequence typing scheme (P. Ruiz-Garbajosa et al., J. Clin. Microbiol. 44:2220-2228, 2006). The isolates originated from geographically widespread medical ...

journal_title：Journal of clinical microbiology

authors： Kawalec M,Pietras Z,Daniłowicz E,Jakubczak A,Gniadkowski M,Hryniewicz W,Willems RJ

更新日期：2007-01-01 00:00:00

abstract：:Investigations of nursery outbreaks of Citrobacter diversus sepsis and meningitis have been hampered by lack of adequate epidemiologic markers for the organism. We studied outer membrane protein profiles from clinical isolates of C. diversus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine whe...

journal_title：Journal of clinical microbiology

authors： Kline MW,Mason EO Jr,Kaplan SL

更新日期：1989-08-01 00:00:00

abstract：:Borrelia burgdorferi expresses a conserved, species-specific 39-kDa protein (P39) that can stimulate antibodies during human infection. To confirm that anti-P39 antibodies are produced consistently in animals exposed to infectious spirochetes, white-footed mice, Peromyscus leucopus, and laboratory white mice, Mus musc...

journal_title：Journal of clinical microbiology

authors： Simpson WJ,Burgdorfer W,Schrumpf ME,Karstens RH,Schwan TG

更新日期：1991-02-01 00:00:00

abstract：:The accuracy of disk susceptibility tests with ampicillin and ampicillin-sulbactam was not improved when the amount of ampicillin was increased from 10 to 20 or 30 micrograms per disk. For testing members of the family Enterobacteriaceae, ampicillin disk tests correlated better with broth microdilution tests when the ...

journal_title：Journal of clinical microbiology

authors： Barry AL,Jones RN

更新日期：1988-04-01 00:00:00

abstract：:Infections due to small-colony variants (SCVs) of Staphylococcus aureus in patients with chronic and recurrent infections are an emerging problem; however, studies with this subpopulation are hampered by the fact that SCVs may exhibit unstable phenotypes, making them difficult to study, particularly in broth media. In...

journal_title：Journal of clinical microbiology

authors： Becker K,Laham NA,Fegeler W,Proctor RA,Peters G,von Eiff C

更新日期：2006-09-01 00:00:00

abstract：:Antibodies to Japanese encephalitis virus (JEV) nonstructural 1 (NS1) protein constitute a marker of natural JEV infection among populations vaccinated with inactivated JE vaccine. In Japan, with few recent human JE cases, the natural infection rate is critical to evaluate the necessity of continuing JE vaccination. A...

journal_title：Journal of clinical microbiology

authors： Konishi E,Shoda M,Ajiro N,Kondo T

更新日期：2004-11-01 00:00:00

abstract：:While the majority of avian influenza virus (AIV) subtypes are classified as low-pathogenicity avian influenza viruses (LPAIV), the H5 and H7 subtypes have the ability to mutate to highly pathogenic avian influenza viruses (HPAIV) in poultry and therefore are the etiological agents of notifiable AIV (NAIV). It is of g...

journal_title：Journal of clinical microbiology

authors： Leijon M,Ullman K,Thyselius S,Zohari S,Pedersen JC,Hanna A,Mahmood S,Banks J,Slomka MJ,Belák S

更新日期：2011-11-01 00:00:00

abstract：:We evaluated quantitative real-time PCR to establish the diagnosis of rotavirus gastroenteritis in a high-disease-burden population in Malawi using enzyme immunoassay as the gold standard diagnostic test. In 146 children with acute gastroenteritis and 65 asymptomatic children, we defined a cutoff point in the threshol...

journal_title：Journal of clinical microbiology

authors： Bennett A,Bar-Zeev N,Jere KC,Tate JE,Parashar UD,Nakagomi O,Heyderman RS,French N,Iturriza-Gomara M,Cunliffe NA

更新日期：2015-06-01 00:00:00

abstract：:Ventilator-associated pneumonia is frequently diagnosed with quantitative cultures of samples obtained by bronchoscopic techniques, a method associated with high costs and potential adverse effects. Quantitative cultures of endotracheal aspirates are easier and cheaper to obtain, and good correlations between the resu...

journal_title：Journal of clinical microbiology

authors： Bergmans DC,Bonten MJ,De Leeuw PW,Stobberingh EE

更新日期：1997-03-01 00:00:00

abstract：:Bordetella pertussis was diagnosed in a human immunodeficiency virus-infected patient by a newly developed method in which bacterial DNA is amplified directly from sputum Gram-stained slides. The validation of the method is described along with an additional new PCR-based assay that can distinguish between B. pertussi...

journal_title：Journal of clinical microbiology

authors： Vielemeyer O,Crouch JY,Edberg SC,Howe JG

更新日期：2004-02-01 00:00:00

abstract：:Mucor ramosissimus Samutsevitsch is presented for the first time as an etiologic agent of cutaneous zygomycosis in a patient with aplastic anemia on immunosuppressive therapy. This report also represents the third case caused by this species reported in the literature. A biopsy taken from a lesion on the patient's thi...

journal_title：Journal of clinical microbiology

authors： Weitzman I,Della-Latta P,Housey G,Rebatta G

更新日期：1993-09-01 00:00:00

abstract：:Electrophoretic karyotypes of atypical isolates of Candida albicans, e.g., strains that were germ tube negative, failed to express proteinase activity, demonstrated low virulence for mice, formed hyperchlamydoconidia, produced hyperhyphae, or were sucrose negative (including the type strain of Candida stellatoidea), w...

journal_title：Journal of clinical microbiology

authors： Mahrous M,Lott TJ,Meyer SA,Sawant AD,Ahearn DG

更新日期：1990-05-01 00:00:00

abstract：:Nocardia brasiliensis, the second most frequently isolated aerobic actinomycete in the clinical laboratory, is usually associated with localized cutaneous infections. However, 22% of 238 N. brasiliensis isolates from the United States and 12% of 66 isolates from Queensland, Australia, which had been collected over a 1...

journal_title：Journal of clinical microbiology

authors： Wallace RJ Jr,Brown BA,Blacklock Z,Ulrich R,Jost K,Brown JM,McNeil MM,Onyi G,Steingrube VA,Gibson J

更新日期：1995-06-01 00:00:00

abstract：:Naturally acquired infection of humans with a marine mammal-associated Brucella sp. has only been reported once previously in a study describing infections of two patients from Peru. We report the isolation and characterization of a strain of Brucella from a New Zealand patient that appears most closely related to str...

journal_title：Journal of clinical microbiology

authors： McDonald WL,Jamaludin R,Mackereth G,Hansen M,Humphrey S,Short P,Taylor T,Swingler J,Dawson CE,Whatmore AM,Stubberfield E,Perrett LL,Simmons G

更新日期：2006-12-01 00:00:00

abstract：:Ten strains of enterotoxigenic Escherichia coli producing heat-labile enterotoxin (LT) were preserved under 12 different conditions. After 1 month, 9 months, and 3 years of preservation, the cultures were recovered and examined for LT production. Preservation of the cultures on Dorset Egg Medium at 4 degrees C and pre...

journal_title：Journal of clinical microbiology

authors： Yoh M,Narita I,Honda T,Miwatani T,Nishibuchi M

更新日期：1991-10-01 00:00:00

abstract：:It has been difficult to obtain pure Pneumocystis carinii antigen either from cultures or from infected lungs for use in producing a specific antibody against P. carinii. This report describes an approach toward producing a monoclonal antibody that bypasses the antigen purification steps. P. carinii infection was deve...

journal_title：Journal of clinical microbiology

authors： Lee CH,Bolinger CD,Bartlett MS,Kohler RB,Wilde CE 3rd,Smith JW

更新日期：1986-03-01 00:00:00

abstract：:Since the discovery of the Norwalk virus (NV) by immune electron microscopy (IEM) in 1972, serologic studies with this virus have relied on particle-positive fecal material from infected volunteers as the source of antigen because it has not been possible to propagate this virus in cell culture. However, the recent cl...

journal_title：Journal of clinical microbiology

authors： Green KY,Lew JF,Jiang X,Kapikian AZ,Estes MK

更新日期：1993-08-01 00:00:00

abstract：:Extended incubation of culture plates was studied to see if the recovery of Bordetella spp. from nasopharyngeal swabs could be improved. Forty-eight Bordetella isolates were recovered from 103 children (overall positive-culture rate, 46.6%) who met the clinical case definition of pertussis. Seven of 44 (16%) B. pertus...

journal_title：Journal of clinical microbiology

authors： Katzko G,Hofmeister M,Church D

更新日期：1996-06-01 00:00:00

abstract：:Mycobacterium tuberculosis complex, Mycobacterium avium, and many other nontuberculous mycobacteria are worldwide distributed microorganisms of major medical and veterinary importance. Considering the growing epidemiologic significance of wildlife-livestock-human interrelation, developing rapid detection tools of high...

journal_title：Journal of clinical microbiology

authors： Sevilla IA,Molina E,Elguezabal N,Pérez V,Garrido JM,Juste RA

更新日期：2015-03-01 00:00:00

abstract：:Mycoplasma hominis most frequently causes diseases of the genitourinary tract. Extragenital infections are uncommon, with almost all occurring in immunosuppressed persons or those predisposed due to trauma or surgery. We present the case of a previously well man who developed an M. hominis-associated parapharyngeal ab...

journal_title：Journal of clinical microbiology

authors： Kennedy KJ,Prince S,Makeham T

更新日期：2009-09-01 00:00:00

abstract：:One of the capabilities developed by bacteria is the ability to gain large fragments of DNA from other bacteria or to lose portions of their own genomes. Among these exchangeable fragments are the genomic islands (GIs). Nine GIs have been identified in Brucella, and genomic island 3 (GI-3) is shared by two pathogenic ...

journal_title：Journal of clinical microbiology

authors： Céspedes S,Salgado P,Valenzuela P,Vidal R,Oñate AA

更新日期：2011-07-01 00:00:00

abstract：:Multidrug-resistant tuberculosis is an increasing health problem worldwide, especially in developing countries. The PCR-UHG-Rif assay, which detects mutations within the rpoB gene associated with rifampin resistance, was evaluated for its ability and reliability to detect and identify drug-resistant Mycobacterium tube...

journal_title：Journal of clinical microbiology

authors： Mayta H,Gilman RH,Arenas F,Valencia T,Caviedes L,Montenegro SH,Ticona E,Ortiz J,Chumpitaz R,Evans CA,Williams DL,Tuberculosis Working Group in Perú.

更新日期：2003-12-01 00:00:00

abstract：:We evaluated two commercial F1 antigen capture-based immunochromatographic dipsticks, Yersinia Pestis (F1) Smart II and Plague BioThreat Alert test strips, in detecting plague bacilli by using whole-blood samples from mice experimentally infected with Yersinia pestis CO92. To assess the specificities of these dipstick...

journal_title：Journal of clinical microbiology

authors： Sha J,Endsley JJ,Kirtley ML,Foltz SM,Huante MB,Erova TE,Kozlova EV,Popov VL,Yeager LA,Zudina IV,Motin VL,Peterson JW,DeBord KL,Chopra AK

更新日期：2011-05-01 00:00:00

abstract：:Between 1999 and 2002, 496 invasive group A streptococcal (GAS) isolates from clinical microbiological departments in Denmark and subsequently 487 (98%) questionnaires from the clinicians treating the patients were received as part of a national surveillance. emm types and streptococcal superantigen (SAg) genes were d...

journal_title：Journal of clinical microbiology

authors： Ekelund K,Skinhøj P,Madsen J,Konradsen HB

更新日期：2005-04-01 00:00:00

abstract：:Four methods for the detection of Trichomonas vaginalis in vaginal secretions from 88 symptomatic patients were compared: wet-mount examination, Kupferberg liquid medium, Hirsch charcoal agar, and the Papanicolaou smear. Hirsch diphasic slants and Kupferberg medium did not significantly differ in sensitivity from dire...

journal_title：Journal of clinical microbiology

authors： Thomason JL,Gelbart SM,Sobun JF,Schulien MB,Hamilton PR

更新日期：1988-09-01 00:00:00

abstract：:Human (n = 33) and canine (n = 53) Campylobacter upsaliensis isolates from seven countries were genotyped by a new amplified fragment length polymorphism method. We observed 100% typeability and high overall diversity. The majority of human strains (23/33) clustered separately from canine strains, indicating that dogs...

journal_title：Journal of clinical microbiology

authors： Damborg P,Guardabassi L,Pedersen K,Kokotovic B

更新日期：2008-04-01 00:00:00

abstract：:The fortuitous finding that Aeromonas spp. grew well on Butzler Campylobacter selective medium (BCSA) in a microaerobic atmosphere at 42 degrees C prompted us to evaluate the performance of BCSA for selective isolation of aeromonads in comparison with ampicillin (30 micrograms/ml) sheep blood agar (ASBA30). Overall re...

journal_title：Journal of clinical microbiology

authors： Misra SK,Bhadra RK,Pal SC,Nair GB

更新日期：1989-02-01 00:00:00

abstract：:The name Psychrobacter immobilis recently has been proposed for a group of chiefly psychrotrophic, aerobic, gram-negative, nonmotile, oxidase-positive coccobacilli commonly found associated with fish, processed meat, and poultry products. This article reports an ocular infection in a 12-day-old newborn who acquired th...

journal_title：Journal of clinical microbiology

authors： Gini GA

更新日期：1990-02-01 00:00:00