Abstract:
:A serotyping scheme for Vibrio vulnificus predicated on the detection of lipopolysaccharide (LPS) antigens is proposed. The serovar O typing scheme used to type V. vulnificus employs polyclonal antisera raised in rabbits immunized with heat-killed whole-cell vaccines. Polyclonal typing sera produced in this manner cross-react with heterologous strains. Affinity purification of polyclonal antisera with LPS affinity columns resolved some of these cross-reactions; however, affinity-purified polyclonal antisera still showed cross-reactions that were nonreciprocal. On the basis of the serological patterns that were obtained with affinity-purified polyclonal antisera, V. vulnificus strains were selected as vaccine strains for production of monoclonal antibody. Spleen cells harvested from BALB/c mice immunized with formalin-killed V. vulnificus cells were fused with SP2/O-Ag 14 myeloma cells. Hybridomas were screened by using LPS and whole-cell enzyme-linked immunosorbent assay to identify clones secreting LPS-specific antibodies. Monoclonal antibodies identified five LPS serological varieties of V. vulnificus and a single serovar each for Vibrio damsela and Vibrio hollisae. No cross-reactions between V. vulnificus and V. hollisae or V. damsela were observed.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Martin SJ,Siebeling RJdoi
10.1128/JCM.29.8.1684-1688.1991subject
Has Abstractpub_date
1991-08-01 00:00:00pages
1684-8issue
8eissn
0095-1137issn
1098-660Xjournal_volume
29pub_type
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.24.6.963-967.1986
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journal_title:Journal of clinical microbiology
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