Identification of clinical coryneform bacterial isolates: comparison of biochemical methods and sequence analysis of 16S rRNA and rpoB genes.

abstract：:A recently described medium (Haemophilus test medium [HTM]) for antimicrobial susceptibility testing of Haemophilus influenzae was evaluated in this study for broth microdilution testing of Streptococcus pneumoniae. A total of 137 clinical isolates was tested against 11 antimicrobial agents, using Mueller-Hinton broth...

journal_title：Journal of clinical microbiology

authors： Jorgensen JH,Maher LA,Howell AW

更新日期：1990-03-01 00:00:00

abstract：:An outbreak of coronavirus disease 2019 (COVID-19) caused by a novel coronavirus (severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2]) began in Wuhan, Hubei, China, in December 2019 and spread rapidly worldwide. The response by the Alberta Precision Laboratories, Public Health Laboratory (ProvLab), AB, Canada...

journal_title：Journal of clinical microbiology

authors： Pabbaraju K,Wong AA,Douesnard M,Ma R,Gill K,Dieu P,Fonseca K,Zelyas N,Tipples GA

更新日期：2020-07-23 00:00:00

abstract：:The region surrounding mecA in methicillin-resistant Staphylococcus aureus (MRSA) is highly variable. We describe an approach for the rapid genotyping of MRSA by assaying for the presence or absence of variable or mobile elements previously shown to be associated with the mecA region. ...

journal_title：Journal of clinical microbiology

authors： Huygens F,Nimmo GR,Schooneveldt J,Munckhof WJ,Giffard PM

更新日期：2002-08-01 00:00:00

abstract：:Clostridioides (Clostridium) difficile infection (CDI) is the most common causative pathogen of health care-associated gastrointestinal infections; however, due to the overlap of clinical symptoms with those of other causes of acute gastroenteritis, the selection of the most appropriate laboratory test is difficult. F...

journal_title：Journal of clinical microbiology

authors： Krutova M,Briksi A,Tkadlec J,Zajac M,Matejkova J,Nyc O,Drevinek P

更新日期：2019-09-24 00:00:00

abstract：:We describe the isolation of Actinobacillus lignieresii and an A. equuli-like bacterium from an infected horse-bite wound in a 22-year-old stable foreman and A. suis from a bite injury in a 35-year-old man who had been attacked by a horse. A. lignieresii was also isolated in pure culture from an infected sheep-bite wo...

journal_title：Journal of clinical microbiology

authors： Peel MM,Hornidge KA,Luppino M,Stacpoole AM,Weaver RE

更新日期：1991-11-01 00:00:00

abstract：:Microsporidia are protozoa parasites responsible for significant gastrointestinal disease in patients infected with human immunodeficiency virus. We evaluated a PCR assay of stool samples, duodenal aspirates, and biopsy specimens from patients with Enterocytozoon bieneusi infection. A 210-bp DNA fragment of the unique...

journal_title：Journal of clinical microbiology

authors： Velásquez JN,Carnevale S,Guarnera EA,Labbé JH,Chertcoff A,Cabrera MG,Rodríguez MI

更新日期：1996-12-01 00:00:00

abstract：:Evaluation of candidate antiviral drugs against Epstein-Barr virus (EBV), human herpesvirus 6 (HHV-6), and HHV-8 is hampered by the lack of convenient laboratory assays. We developed real-time quantitative PCR assays performed on supernatants of lymphoma cell lines and determined the 50% inhibitory concentrations (IC5...

journal_title：Journal of clinical microbiology

authors： Bounaadja L,Piret J,Goyette N,Boivin G

更新日期：2013-04-01 00:00:00

abstract：:A singleplex PCR assay using a single primer pair targeting the putative sugar transporter gene was developed here to distinguish Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii according to the distinct size of the amplicon. The interspecies and intravarietal hybr...

journal_title：Journal of clinical microbiology

authors： Feng X,Fu X,Ling B,Wang L,Liao W,Yao Z

更新日期：2013-06-01 00:00:00

abstract：:Sequencing-based pathogen identification directly from clinical specimens requires time-consuming interpretation, especially with mixed chromatograms when multiple microorganisms are detected. We assessed RipSeq Mixed software for human papillomavirus (HPV) genotyping by comparison to the linear array HPV genotyping a...

journal_title：Journal of clinical microbiology

authors： Tardif KD,Simmon KE,Kommedal O,Pyne MT,Schlaberg R

更新日期：2013-04-01 00:00:00

abstract：:In this study, we determined the sequences of four intimin variant genes detected in attaching and effacing Escherichia coli isolates of human origin. Three of them were novel and were designated eae-eta (eta), eae-iota (iota), and eae-kappa (kappa). The fourth was identical to the recently described eae-zeta (zeta), ...

journal_title：Journal of clinical microbiology

authors： Zhang WL,Köhler B,Oswald E,Beutin L,Karch H,Morabito S,Caprioli A,Suerbaum S,Schmidt H

更新日期：2002-12-01 00:00:00

abstract：:Several media were used to evaluate the hemolytic activity of Listeria monocytogenes, and this property was used along with the API Listeria system to identify Listeria spp. All L. monocytogenes strains were identified correctly with this system, and blood agar base no. 2 and Columbia blood agar base supplemented with...

journal_title：Journal of clinical microbiology

authors： Fujisawa T,Mori M

更新日期：1994-04-01 00:00:00

abstract：:We report a case of septic arthritis of a native knee joint due to Corynebacterium striatum, a rare and unusual cause of septic arthritis of native joints. The isolate was identified by a combination of phenotypic, mass spectrometric, and nucleic acid-based assays and exhibited high-level resistance to most antimicrob...

journal_title：Journal of clinical microbiology

authors： Westblade LF,Shams F,Duong S,Tariq O,Bulbin A,Klirsfeld D,Zhen W,Sakaria S,Ford BA,Burnham CA,Ginocchio CC

更新日期：2014-05-01 00:00:00

abstract：:During a 1-year survey of Shiga toxin-producing Escherichia coli (STEC) prevalence in central France, 2,143 samples were investigated by PCR for Shiga toxin-encoding genes. A total of 330 (70%) of 471 fecal samples collected from healthy cattle at the Clermont-Ferrand slaughterhouse, 47 (11%) of 411 beef samples, 60 (...

journal_title：Journal of clinical microbiology

authors： Pradel N,Livrelli V,De Champs C,Palcoux JB,Reynaud A,Scheutz F,Sirot J,Joly B,Forestier C

更新日期：2000-03-01 00:00:00

abstract：:A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregati...

journal_title：Journal of clinical microbiology

authors： Toma C,Lu Y,Higa N,Nakasone N,Chinen I,Baschkier A,Rivas M,Iwanaga M

更新日期：2003-06-01 00:00:00

abstract：:A fluorogenic assay for the detection of beta-glucosidase was developed as part of a simplified conventional method to distinguish Staphylococcus warneri and Staphylococcus hominis isolated from bovine body sites. The assay is based on the fact that strains of S. warneri produce beta-glucosidase, while strains of S. h...

journal_title：Journal of clinical microbiology

authors： White DG,Harmon RJ,Langlois BE

更新日期：1990-03-01 00:00:00

abstract：:Many bunyaviruses of the Bunyamwera and California serogroups are medically important human pathogens. The development of an effective technique to detect the viruses by using molecular biologic tools, such as PCR, improves not only clinical diagnosis but also virologic surveillance of mosquito vectors in the field. I...

journal_title：Journal of clinical microbiology

authors： Kuno G,Mitchell CJ,Chang GJ,Smith GC

更新日期：1996-05-01 00:00:00

abstract：:The ability to isolate and propagate influenza virus is an essential tool for the yearly surveillance of circulating virus strains and to ensure accurate clinical diagnosis for appropriate treatment. The suitability of MDCK-SIAT1 cells, engineered to express increased levels of alpha-2,6-linked sialic acid receptors, ...

journal_title：Journal of clinical microbiology

authors： Oh DY,Barr IG,Mosse JA,Laurie KL

更新日期：2008-07-01 00:00:00

abstract：:Amplification of human papillomavirus (HPV) DNA by L1 consensus primer systems (e.g., MY09/11 or GP5(+)/6(+)) can detect as few as 10 to 100 molecules of HPV targets from a genital sample. However, genotype determination by dot blot hybridization is laborious and requires at least 27 separate hybridizations for substa...

journal_title：Journal of clinical microbiology

authors： Gravitt PE,Peyton CL,Apple RJ,Wheeler CM

更新日期：1998-10-01 00:00:00

abstract：:Automated treponemal immunoassays are used for syphilis screening with the reverse-sequence algorithm; discordant results (e.g., enzyme immunoassay [EIA] reactive and reactive plasma reagin [RPR] nonreactive) are resolved with a second treponemal test. We conducted a study to determine automated immunoassay signal str...

journal_title：Journal of clinical microbiology

authors： Fakile YF,Jost H,Hoover KW,Gustafson KJ,Novak-Weekley SM,Schapiro JM,Tran A,Chow JM,Park IU

更新日期：2017-12-26 00:00:00

abstract：:The performance of CHROMagar STEC and CHROMagar STEC O104 (CHROMagar Microbiology, Paris, France) media for the detection of Shiga toxin-producing Escherichia coli (STEC) was assessed with 329 stool specimens collected over 14 months from patients with suspected STEC infections (June 2011 to August 2012). The CHROMaga...

journal_title：Journal of clinical microbiology

authors： Gouali M,Ruckly C,Carle I,Lejay-Collin M,Weill FX

更新日期：2013-03-01 00:00:00

abstract：:Cryptococcus neoformans var. gattii is a causative agent of cryptococcosis and is thought to have a specific ecological association with a number of Eucalyptus species in Australia. However, the role that the tree plays in the life cycle of the fungus and the nature of the infectious propagule are not well understood....

journal_title：Journal of clinical microbiology

authors： Halliday CL,Carter DA

更新日期：2003-02-01 00:00:00

abstract：:Pasteurella multocida is a small nonmotile gram-negative coccobacillus that is found in the nasopharynx and gastrointestinal tract of many wild and domesticated animals. In humans it most commonly causes cellulitis and localized superficial skin abscesses following an animal bite or scratch. The respiratory tract is t...

journal_title：Journal of clinical microbiology

authors： Chen HI,Hulten K,Clarridge JE 3rd

更新日期：2002-09-01 00:00:00

abstract：:In this study we evaluated the ability of three commercial methods, API 20A (Analytab Products, Plainview, N.Y.), Minitek (BBL Microbiology Systems, Cockeysville, Md.), and Anaerobe-Tek (Flow Laboratories, Inc., McLean, Va.), to accurately identify 165 recent clinical and 38 stock isolates of anaerobic bacteria withou...

journal_title：Journal of clinical microbiology

authors： Appelbaum PC,Kaufmann CS,Keifer JC,Venbrux HJ

更新日期：1983-09-01 00:00:00

abstract：:Toxin-specific enzyme immunoassays, cytotoxicity assays, and PCR were used to analyze 48 toxin A-negative, toxin B-positive Clostridium difficile isolates from various geographical sites around the world. All the isolates were negative by the TOX-A TEST and positive by the TOX A/B TEST. A deletion of approximately 1.7...

journal_title：Journal of clinical microbiology

authors： Moncrief JS,Zheng L,Neville LM,Lyerly DM

更新日期：2000-08-01 00:00:00

abstract：:Mycobacterium tuberculosis that is resistant to both isoniazid (INH) and rifampin (RIF) is spreading. It has become a public health problem in part because the standard culture methods used to determine the appropriate treatment regimen for patients often take months following the presumptive diagnosis of tuberculosis...

journal_title：Journal of clinical microbiology

authors： Massire C,Ivy CA,Lovari R,Kurepina N,Li H,Blyn LB,Hofstadler SA,Khechinashvili G,Stratton CW,Sampath R,Tang YW,Ecker DJ,Kreiswirth BN

更新日期：2011-03-01 00:00:00

abstract：:We compared 14 molecular assays for their ability to detect the Mycobacterium tuberculosis complex in bronchoalveolar lavage fluid samples. Three approaches were followed. First, by using DNA from Mycobacterium bovis BCG, we determined the detection limits of the assays using routine molecular methods. Second, in orde...

journal_title：Journal of clinical microbiology

authors： Akkerman OW,van der Werf TS,de Boer M,de Beer JL,Rahim Z,Rossen JW,van Soolingen D,Kerstjens HA,van der Zanden AG

更新日期：2013-11-01 00:00:00

abstract：:A total of 375 Escherichia coli O157 strains were tested by colony hybridization with the eae probe from the central portion of the eaeA gene of the classical enteropathogenic E. coli strain E2348/69. They were also tested with a probe, eaeO157, from the C-terminal end of the eae gene homolog from a Vero cytotoxin (VT...

journal_title：Journal of clinical microbiology

authors： Willshaw GA,Scotland SM,Smith HR,Cheasty T,Thomas A,Rowe B

更新日期：1994-04-01 00:00:00

abstract：:The protease phenotypes expressed by isolates of Pseudomonas aeruginosa from cystic fibrosis (CF) patients were evaluated. The majority of isolates tested produced elastase (65%) or alkaline protease (64%) or both. The mucoid phenotype expressed by many CF isolates of P. aeruginosa did not absolutely restrict the expr...

journal_title：Journal of clinical microbiology

authors： Jagger KS,Bahner DR,Warren RL

更新日期：1983-01-01 00:00:00

abstract：:Mycobacterium abscessus, which consists of the two subspecies M. abscessus subspecies abscessus and M. abscessus subspecies bolletii, can produce rough or smooth colony morphologies. Here we analyzed 50 M. abscessus isolates cultured from the respiratory specimens of 34 patients, 28 (82%) of whom had cystic fibrosis (...

journal_title：Journal of clinical microbiology

authors： Rüger K,Hampel A,Billig S,Rücker N,Suerbaum S,Bange FC

更新日期：2014-01-01 00:00:00

abstract：:A disease similar to ulcerative colitis in humans has been identified in cotton-top tamarins (CTTs) in captivity. The clinical signs include weight loss, diarrhea, and rectal bleeding with the pathological features and biochemical abnormalities of ulcerative colitis. Approximately 25 to 40% of these animals develop co...

journal_title：Journal of clinical microbiology

authors： Saunders KE,Shen Z,Dewhirst FE,Paster BJ,Dangler CA,Fox JG

更新日期：1999-01-01 00:00:00