Abstract:
:In this study, we determined the sequences of four intimin variant genes detected in attaching and effacing Escherichia coli isolates of human origin. Three of them were novel and were designated eae-eta (eta), eae-iota (iota), and eae-kappa (kappa). The fourth was identical to the recently described eae-zeta (zeta), isolated from a bovine E. coli O84:NM isolate. We compared these sequences with those of published intimin-alpha, intimin-beta, intimin-gamma1, intimin-gamma2, intimin- epsilon, and intimin-theta alleles. Sequence analysis of these 10 intimin alleles confirmed extensive genetic diversity within the intimin gene family in E. coli. The genetic diversity was more prominent in the 3' region (starting at bp 2,112), which encodes the binding domain of intimin. Phylogenetic analyses revealed four groups of closely related intimin genes: alpha and zeta; beta and kappa; gamma1 and gamma2/theta; and epsilon and eta. Calculation of homoplasy ratios of sequences of the 5' region of eae (positions 1 to 2,111) revealed evidence for intragenic recombination. Split decomposition analysis also indicates that recombination events have played a role in the evolutionary history of eae. In conclusion, we recommend an eae nomenclature system based on the Greek alphabet and provide an updated PCR scheme for amplification and typing of E. coli eae.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Zhang WL,Köhler B,Oswald E,Beutin L,Karch H,Morabito S,Caprioli A,Suerbaum S,Schmidt Hdoi
10.1128/jcm.40.12.4486-4492.2002subject
Has Abstractpub_date
2002-12-01 00:00:00pages
4486-92issue
12eissn
0095-1137issn
1098-660Xjournal_volume
40pub_type
杂志文章abstract::Resistance to antimonial drugs has been documented in Leishmania isolates transmitted in South America, Europe, and Asia. The frequency and distribution of resistance to these and other antileishmanial drugs are unknown. Technical constraints have limited the assessment of drug susceptibility of clinical strains of Le...
journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.00216-12
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.28.6.1395-1397.1990
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.25.2.216-221.1987
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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abstract::The purpose of this investigation was to ascertain the lipolytic activities (specifically, esterase) of those species of Candida that are most commonly isolated from human infections. Eight species of Candida were surveyed for their ability to hydrolyze various polyoxyethylene sorbitan compounds (Tweens). Of the 64 is...
journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.25.8.1516-1518.1987
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.33.10.2707-2709.1995
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2011-05-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.37.1.35-38.1999
更新日期:1999-01-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2009-12-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1977-02-01 00:00:00
abstract::A proficiency review of antituberculous drug susceptibility testing (DST) was undertaken by the regional tuberculosis reference laboratories of the Western Pacific Region of WHO to evaluate the performance of national reference laboratories (NRLs) and to ensure that the results from the participating laboratories are ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00784-07
更新日期:2007-11-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2008-06-01 00:00:00
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journal_title:Journal of clinical microbiology
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abstract::The specificity of Mycobacterium kansasii anti-mycoside A antiserum prepared in rabbits injected with purified samples of the phenolic glycolipid was evaluated by an enzyme-linked immunosorbent assay. Chloroform-methanol extracts from representative strains of 23 mycobacterial species and 50 strains of M. kansasii sho...
journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.34.4.973-974.1996
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.17.1.63-67.1983
更新日期:1983-01-01 00:00:00
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章,多中心研究
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更新日期:2007-04-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2003-10-01 00:00:00
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journal_title:Journal of clinical microbiology
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更新日期:2008-01-01 00:00:00
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journal_title:Journal of clinical microbiology
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更新日期:2011-03-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.32.8.2013-2015.1994
更新日期:1994-08-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2004-09-01 00:00:00