Surveillance of susceptibility testing methodologies for Haemophilus influenzae in Canada, including evaluation of disk diffusion test.

abstract：:A newly developed commercial serological test (Iatron Laboratories, Inc., Tokyo, Japan) for the rapid identification of medically important species of Candida was evaluated against the API 20C (Analytab Products, Plainview, N.Y.) and the standard Wickerham assimilation and fermentation procedures. Our results indicate...

journal_title：Journal of clinical microbiology

authors： Shinoda T,Kaufman L,Padhye AA

更新日期：1981-03-01 00:00:00

abstract：:Actinobacillus actinomycetemcomitans is an important pathogen in the etiology of severe periodontitis. For epidemiological studies on the prevalence of certain pathogenic clones and transmission of this bacterium, adequate typing methods are necessary. The purpose of this study was to compare six different typing meth...

journal_title：Journal of clinical microbiology

authors： van Steenbergen TJ,Bosch-Tijhof CJ,van Winkelhoff AJ,Gmür R,de Graaff J

更新日期：1994-11-01 00:00:00

abstract：:The API STAPH-IDENT system was compared with conventional methods for the identification of 14 Staphylococcus species. Conventional methods included the Kloos and Schleifer simplified scheme and DNA-DNA hybridization. The API STAPH-IDENT strip utilizes a battery of 10 miniaturized biochemical tests, including alkaline...

journal_title：Journal of clinical microbiology

authors： Kloos WE,Wolfshohl JF

更新日期：1982-09-01 00:00:00

abstract：:Helicobacter pylori strains display remarkable genetic diversity, and the presence of strains bearing the toxigenic vacA s1 allele, a complete cag pathogenicity island (PAI), cagA alleles containing multiple EPIYA phosphorylation sites, and expressing the BabA adhesin correlates with development of gastroduodenal dise...

journal_title：Journal of clinical microbiology

authors： Talarico S,Gold BD,Fero J,Thompson DT,Guarner J,Czinn S,Salama NR

更新日期：2009-06-01 00:00:00

abstract：:The RD2 region of the internalization-associated gene prtF1, which encodes the fibronectin-binding repeat domain type 2 of protein F1, plays a crucial role in the entry of group A streptococci (GAS) into epithelial cells. A molecular study of the variability of the RD2 region was carried out with 77 independent Italia...

journal_title：Journal of clinical microbiology

authors： Spinaci C,Magi G,Zampaloni C,Vitali LA,Paoletti C,Catania MR,Prenna M,Ferrante L,Ripa S,Varaldo PE,Facinelli B

更新日期：2004-02-01 00:00:00

abstract：:Seventeen Minnesota and Wisconsin dogs with granulocytic ehrlichosis were studied. The diagnoses were made by finding ehrlichia morulae in peripheral blood neutrophils. Eight dogs were studied retrospectively, and nine dogs were studied prospectively. The medical records of all dogs were reviewed. Eighty-eight percent...

journal_title：Journal of clinical microbiology

authors： Greig B,Asanovich KM,Armstrong PJ,Dumler JS

更新日期：1996-01-01 00:00:00

abstract：:A method for extracting poliovirus (PV) from stool extracts was developed. Magnetic nanoparticles sensitized with soluble PV receptor efficiently extracted PV pseudovirus (>99% extraction) or endogenous infectious PVs (>90% extraction) from stool extracts. This method would be useful for extraction of PV from crude bi...

journal_title：Journal of clinical microbiology

authors： Arita M

更新日期：2013-08-01 00:00:00

abstract：:We cloned and characterized a phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) gene from Burkholderia pseudomallei. DNA sequence analysis of the gene indicated an open reading frame coding for 700 amino acids with a 34-amino-acid signal peptide. When cleaved, this yields a secreted 73-kDa mature protein. The d...

journal_title：Journal of clinical microbiology

authors： Korbsrisate S,Suwanasai N,Leelaporn A,Ezaki T,Kawamura Y,Sarasombath S

更新日期：1999-11-01 00:00:00

abstract：:To determine the excretion dynamics and genotypic characteristics of rotavirus A (RVA), a longitudinal observational study was performed in 10 pigs from 3 litters at a farrow-to-finish farm. A total of 400 fecal samples were directly collected from the rectums of individual pigs (aged 7 to 217 days) at 3- to 14-day in...

journal_title：Journal of clinical microbiology

authors： Miyazaki A,Kuga K,Suzuki T,Tsunemitsu H

更新日期：2012-06-01 00:00:00

abstract：:Genetic diversity among Borrelia burgdorferi isolates recovered from the skin of Lyme disease patients was assessed by ribosomal DNA (rDNA) spacer restriction fragment length polymorphism analysis, genomic restriction site polymorphism analysis, and plasmid content analysis. There was a significant association between...

journal_title：Journal of clinical microbiology

authors： Iyer R,Liveris D,Adams A,Nowakowski J,McKenna D,Bittker S,Cooper D,Wormser GP,Schwartz I

更新日期：2001-08-01 00:00:00

abstract：:We describe a multiplex reverse transcription-PCR (m-RT-PCR) assay that is able to detect and differentiate all known human parainfluenza viruses (HPIVs). Serial dilution experiments with reference strains that compared cell culture isolation and m-RT-PCR showed sensitivities ranging from 0.0004 50% tissue culture inf...

journal_title：Journal of clinical microbiology

authors： Aguilar JC,Pérez-Breña MP,García ML,Cruz N,Erdman DD,Echevarría JE

更新日期：2000-03-01 00:00:00

abstract：:We determined the frequency with which human immunodeficiency virus (HIV) peripheral blood mononuclear cell cultures convert from positive to negative in subjects enrolled in a substudy of AIDS Clinical Trials Group (ACTG) 320, which compared the efficacy of treatment with a combination of indinavir, zidovudine, and l...

journal_title：Journal of clinical microbiology

authors： Demeter LM,Bosch RJ,Coombs RW,Fiscus S,Bremer J,Johnson VA,Erice A,Jackson JB,Spector SA,Squires KM,Fischl MA,Hughes MD,Hammer SM

更新日期：2002-06-01 00:00:00

abstract：:The present study describes the use of the automated BACTEC 9240 blood culture system, the Serum Separator Tube (SST), and the BD PHOENIX Automated Microbiology System in combination for the direct identification and antimicrobial susceptibility testing (AST) of gram-negative rods (GNRs) from positive blood cultures (...

journal_title：Journal of clinical microbiology

authors： Funke G,Funke-Kissling P

更新日期：2004-04-01 00:00:00

abstract：:A new approach, short-oligonucleotide-ligation assay on DNA chip (SOLAC), is developed to detect mutations in rifampin-resistant Mycobacterium tuberculosis. The method needs only four common probes to detect 15 mutational variants of the rpoB gene within 12 h. Fifty-five rifampin-resistant M. tuberculosis isolates wer...

journal_title：Journal of clinical microbiology

authors： Deng JY,Zhang XE,Lu HB,Liu Q,Zhang ZP,Zhou YF,Xie WH,Fu ZJ

更新日期：2004-10-01 00:00:00

abstract：:The protease phenotypes expressed by isolates of Pseudomonas aeruginosa from cystic fibrosis (CF) patients were evaluated. The majority of isolates tested produced elastase (65%) or alkaline protease (64%) or both. The mucoid phenotype expressed by many CF isolates of P. aeruginosa did not absolutely restrict the expr...

journal_title：Journal of clinical microbiology

authors： Jagger KS,Bahner DR,Warren RL

更新日期：1983-01-01 00:00:00

abstract：:The correlation of β-glucan (BG) levels with clinical outcomes in invasive candidiasis (IC) remains unknown. Patients with proven IC were followed prospectively from diagnosis to outcome with twice-weekly serum BG sampling. Correlation of BG with clinical outcome was assessed in each patient. BG levels tend to decreas...

journal_title：Journal of clinical microbiology

authors： Sims CR,Jaijakul S,Mohr J,Rodriguez J,Finkelman M,Ostrosky-Zeichner L

更新日期：2012-06-01 00:00:00

abstract：:Vibrio vulnificus was isolated from a bacteremic patient. This strain, together with other isolates of V. vulnificus, was compared with V. alginolyticus, V. fluvialis, and V. parahaemolyticus with regard to growth characteristics on enteric agar media (enabling isolation and identification) and production of exoenzyme...

journal_title：Journal of clinical microbiology

authors： Desmond EP,Janda JM,Adams FI,Bottone EJ

更新日期：1984-02-01 00:00:00

abstract：:Real-time PCR analysis of the total bacterial load in advanced carious lesions has shown that the total load exceeds the number of cultivable bacteria. This suggests that an unresolved complexity exists in bacteria associated with advanced caries. In this report, the profile of the microflora of carious dentine was ex...

journal_title：Journal of clinical microbiology

authors： Chhour KL,Nadkarni MA,Byun R,Martin FE,Jacques NA,Hunter N

更新日期：2005-02-01 00:00:00

abstract：:Iodophor and alcohol pledgets were compared with the Medi-Flex Prep Kit II for skin disinfection before venipuncture. Of 12,367 blood cultures collected, 6,362 were done with conventional pledgets and 6, 005 were done with Medi-Flex kits. Contamination occurred in 351 of 6,362 blood cultures (5.5%; range, 3.7 to 8.1%)...

journal_title：Journal of clinical microbiology

authors： Wilson ML,Weinstein MP,Mirrett S,Reimer LG,Fernando C,Meredith FT,Reller LB

更新日期：2000-12-01 00:00:00

abstract：:A new commercial method intended to exclude the presence of Salmonella spp., Shigella spp., and Yersinia enterocolitica and to presumptively identify Salmonella isolates within 2 h after primary isolation from stool specimens was evaluated. This system is marketed in Europe as API Z and in the United States as Rapid S...

journal_title：Journal of clinical microbiology

authors： Teti G,Burdash NM,Zamboni C,Fava C,Tomasello F,Mastroeni P

更新日期：1984-12-01 00:00:00

abstract：:It has been shown by analysis with monoclonal and polyclonal antibodies that outer surface protein C (OspC) of Borrelia burgdorferi sensu lato is highly heterogeneous. To determine if the heterogeneity has a genetic basis, the genes of 18 different B. burgdorferi sensu lato strains have been amplified by PCR, cloned, ...

journal_title：Journal of clinical microbiology

authors： Jauris-Heipke S,Liegl G,Preac-Mursic V,Rössler D,Schwab E,Soutschek E,Will G,Wilske B

更新日期：1995-07-01 00:00:00

abstract：:In the present study, a novel broad-range real-time PCR was developed for the rapid detection of human pathogenic fungi. The assay targets a part of the 28S large-subunit ribosomal RNA (rDNA) gene. We investigated its application for the most important human pathogenic fungal genera, including Aspergillus, Candida, Cr...

journal_title：Journal of clinical microbiology

authors： Vollmer T,Störmer M,Kleesiek K,Dreier J

更新日期：2008-06-01 00:00:00

abstract：:Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease with a high case fatality risk and is caused by the SFTS virus (SFTSV). A retrospective study conducted after the first identification of an SFTS patient in Japan revealed that SFTS is endemic to the region, and the virus exists indig...

journal_title：Journal of clinical microbiology

authors： Yoshikawa T,Fukushi S,Tani H,Fukuma A,Taniguchi S,Toda S,Shimazu Y,Yano K,Morimitsu T,Ando K,Yoshikawa A,Kan M,Kato N,Motoya T,Kuzuguchi T,Nishino Y,Osako H,Yumisashi T,Kida K,Suzuki F,Takimoto H,Kitamoto H,Ma

更新日期：2014-09-01 00:00:00

abstract：:There is a lack of consensus among investigators who use a variety of immunoassay techniques (e.g., enzyme-linked immunosorbent assay [ELISA] and radioimmunoassay) regarding the protocols for describing and forming standard reference or calibration curves and interpolating serum antibody concentrations. This confounds...

journal_title：Journal of clinical microbiology

authors： Plikaytis BD,Holder PF,Pais LB,Maslanka SE,Gheesling LL,Carlone GM

更新日期：1994-10-01 00:00:00

abstract：:Amplification of human papillomavirus (HPV) DNA by L1 consensus primer systems (e.g., MY09/11 or GP5(+)/6(+)) can detect as few as 10 to 100 molecules of HPV targets from a genital sample. However, genotype determination by dot blot hybridization is laborious and requires at least 27 separate hybridizations for substa...

journal_title：Journal of clinical microbiology

authors： Gravitt PE,Peyton CL,Apple RJ,Wheeler CM

更新日期：1998-10-01 00:00:00

abstract：:Aeromonas caviae was recovered as the sole potential enteric pathogen from the stools of 14 of 17 symptomatic children (10 younger than 1 year of age) while Aeromonas hydrophila, Aeromonas sobria, and Plesiomonas shigelloides were isolated once each. The infants from whom A. caviae was isolated all presented with a wa...

journal_title：Journal of clinical microbiology

authors： Namdari H,Bottone EJ

更新日期：1990-05-01 00:00:00

abstract：:Four of eleven affected dogs died despite aggressive treatment during a 2015 focal outbreak of hemorrhagic gastroenteritis following a stay in a pet housing facility. Routine diagnostic investigations failed to identify a specific cause. Virus isolation from fresh necropsy tissues yielded a calicivirus with sequence h...

journal_title：Journal of clinical microbiology

authors： Renshaw RW,Griffing J,Weisman J,Crofton LM,Laverack MA,Poston RP,Duhamel GE,Dubovi EJ

更新日期：2018-04-25 00:00:00

abstract：:Bovine babesiosis is a serious threat to the cattle industry. We prepared blood DNA samples from 13 cattle with clinical babesiosis from the Badulla (n = 8), Jaffna (n = 3), and Kilinochchi (n = 2) districts in Sri Lanka. These DNA samples tested positive in PCR assays specific for Babesiabovis (n = 9), Babesia bigemi...

journal_title：Journal of clinical microbiology

authors： Sivakumar T,Tuvshintulga B,Zhyldyz A,Kothalawala H,Yapa PR,Kanagaratnam R,Vimalakumar SC,Abeysekera TS,Weerasingha AS,Yamagishi J,Igarashi I,Silva SSP,Yokoyama N

更新日期：2018-10-25 00:00:00

abstract：:The incidence of vancomycin resistance among enterococci is increasing in the United States and elsewhere in the world, but automated susceptibility testing methods have difficulty detecting resistance expressed by certain strains. The agar screening method described by Willey et al. (B. M. Willey, B. N. Kreiswirth, A...

journal_title：Journal of clinical microbiology

authors： Swenson JM,Clark NC,Ferraro MJ,Sahm DF,Doern G,Pfaller MA,Reller LB,Weinstein MP,Zabransky RJ,Tenover FC

更新日期：1994-07-01 00:00:00

abstract：:Molecular analysis of Mycobacterium ulcerans has revealed two new insertion sequences (ISs), IS2404 and IS2606. IS2404 was identified by complete sequencing of a previously described repetitive DNA segment from M. ulcerans. This element is 1,274 bp long, contains 12-bp inverted repeats and a single open reading frame ...

journal_title：Journal of clinical microbiology

authors： Stinear T,Ross BC,Davies JK,Marino L,Robins-Browne RM,Oppedisano F,Sievers A,Johnson PD

更新日期：1999-04-01 00:00:00