Genetic Analysis of Babesia Isolates from Cattle with Clinical Babesiosis in Sri Lanka.

Abstract:

:Bovine babesiosis is a serious threat to the cattle industry. We prepared blood DNA samples from 13 cattle with clinical babesiosis from the Badulla (n = 8), Jaffna (n = 3), and Kilinochchi (n = 2) districts in Sri Lanka. These DNA samples tested positive in PCR assays specific for Babesiabovis (n = 9), Babesia bigemina (n = 9), and Babesiaovata (n = 1). Twelve cattle were positive for B. bovis and/or B. bigemina One cow was negative for the tested Babesia species but was positive for Babesia on microscopic examination; the phylogenetic positions of 18S rRNA and cytochrome oxidase subunit III gene sequences suggested that the cow was infected with Babesia sp. Mymensingh, which was recently reported from a healthy cow in Bangladesh. We then developed a novel Babesia sp. Mymensingh-specific PCR assay and obtained positive results for one other sample. Analysis of gene sequences from the cow with positive B. ovata-specific PCR results demonstrated that the animal was infected not with B. ovata but with Babesia sp. Hue-1, which was recently reported from asymptomatic cattle in Vietnam. The virulence of Babesia sp. Hue-1 is unclear, as the cow was coinfected with B. bovis and B. bigemina However, Babesia sp. Mymensingh probably causes severe clinical babesiosis, as it was the sole Babesia species detected in a clinical case. The present study revealed the presence of two bovine Babesia species not previously reported in Sri Lanka, plus the first case of severe bovine babesiosis caused by a Babesia species other than B. bovis, B. bigemina, and Babesiadivergens.

journal_name

J Clin Microbiol

authors

Sivakumar T,Tuvshintulga B,Zhyldyz A,Kothalawala H,Yapa PR,Kanagaratnam R,Vimalakumar SC,Abeysekera TS,Weerasingha AS,Yamagishi J,Igarashi I,Silva SSP,Yokoyama N

doi

10.1128/JCM.00895-18

subject

Has Abstract

pub_date

2018-10-25 00:00:00

issue

11

eissn

0095-1137

issn

1098-660X

pii

JCM.00895-18

journal_volume

56

pub_type

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