Development of a Plasmodium PCR for monitoring efficacy of antimalarial treatment.

Abstract:

:We report in this work a highly sensitive and nonradioactive PCR method for the detection of the four species of parasite causing human malaria. Plasmodium-specific primers corresponding to the small-subunit rRNA genes of the malaria parasite were used, and a 291-bp fragment was amplified. Our results showed a high specificity for the four human Plasmodium species, and we were able to detect one parasite in 50 microl of whole blood. The responses of 12 patients infected with Plasmodium falciparum to antimalarial therapy were monitored by PCR diagnosis and examination of thick blood film for at least 20 min by an experienced microscopist. For one patient this study allowed early diagnosis of therapeutic failure, confirmed 7 days later by examination of the thick blood film. A total of 134 samples were examined; 94 were positive by PCR, and among these 68 were positive by thick blood film examination. The sensitivity of the thick blood film was 72.3% compared to PCR and 60.7% compared to dot blot hybridization.

journal_name

J Clin Microbiol

authors

Ciceron L,Jaureguiberry G,Gay F,Danis M

doi

10.1128/JCM.37.1.35-38.1999

subject

Has Abstract

pub_date

1999-01-01 00:00:00

pages

35-8

issue

1

eissn

0095-1137

issn

1098-660X

journal_volume

37

pub_type

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