Selective amplification of abequose and paratose synthase genes (rfb) by polymerase chain reaction for identification of Salmonella major serogroups (A, B, C2, and D).

abstract：:A latex agglutination test (LAT) based on polystyrene beads sensitized with inactivated avian influenza virus H5N1 particles was developed. Compared with the hemagglutination inhibition test, the sensitivity and specificity of the LAT were 88.8 and 97.6%, respectively, in detecting 830 serum samples from vaccinated ch...

journal_title：Journal of clinical microbiology

authors： Xu X,Jin M,Yu Z,Li H,Qiu D,Tan Y,Chen H

更新日期：2005-04-01 00:00:00

abstract：:A diagnostic test for feline infectious peritonitis virus (FIPV) infection based on a nested PCR (nPCR) assay was developed and tested with FIPV, feline enteric coronavirus (FECV), canine coronavirus (CCV), and transmissible gastroenteritis virus (TGEV) and clinical fluid samples from cats with effusive feline infecti...

journal_title：Journal of clinical microbiology

authors： Gamble DA,Lobbiani A,Gramegna M,Moore LE,Colucci G

更新日期：1997-03-01 00:00:00

abstract：:We retrospectively analyzed epidemiologic information associated with 22 cultures of Neisseria elongata subsp. nitroreducens (formerly CDC group M-6) submitted to the Microbial Diseases Laboratory, California Department of Health Services, Berkeley, over a 16-year period. The most common illnesses noted with this bact...

journal_title：Journal of clinical microbiology

authors： Wong JD,Janda JM

更新日期：1992-03-01 00:00:00

abstract：:The studies reported here were undertaken to assess the ability of the outer membrane proteins (OMPs) of Salmonella typhi to induce a humoral immune response in humans with typhoid fever. OMPs were isolated with the nonionic detergent Triton X-100 and were found to be contaminated with approximately 4% lipopolysacchar...

journal_title：Journal of clinical microbiology

authors： Ortiz V,Isibasi A,García-Ortigoza E,Kumate J

更新日期：1989-07-01 00:00:00

abstract：:The Streptococcus bovis group has undergone significant taxonomic changes over the past 2 decades with the advent of new identification methods with higher discriminatory power. Although the current classification system is not yet embraced by all researchers in the field and debate remains over the performance of mol...

journal_title：Journal of clinical microbiology

authors： Dekker JP,Lau AF

更新日期：2016-07-01 00:00:00

abstract：:Fungal infection of the thyroid is rare. Most reported cases have involved Aspergillus, Coccidioides, and Candida species in the setting of disseminated disease. Infection of the thyroid with Histoplasma capsulatum is rarely reported as part of disseminated disease, even in geographic areas where histoplasmosis is end...

journal_title：Journal of clinical microbiology

authors： Goldani LZ,Klock C,Diehl A,Monteiro AC,Maia AL

更新日期：2000-10-01 00:00:00

abstract：:It is not always possible to culture feces immediately, and appropriate methods for transport of human specimens, unlike those from animals, have not been fully evaluated. Therefore, we took serial subcultures in two phases from six transport media inoculated with human diarrheal stools known to be positive for Campyl...

journal_title：Journal of clinical microbiology

authors： Wang WL,Reller LB,Smallwood B,Luechtefeld NW,Blaser MJ

更新日期：1983-10-01 00:00:00

abstract：:Quantitation of hepatitis C virus (HCV) RNA in plasma and serum samples is a costly procedure in both time and reagents. Additionally, cell-associated viral RNA may not be detected. This study evaluated the accuracy of HCV RNA quantitation in small-volume whole-blood (WB) samples, which would be appropriate for point-...

journal_title：Journal of clinical microbiology

authors： Bruns T,Steinmetzer K,Ermantraut E,Stallmach A

更新日期：2009-10-01 00:00:00

abstract：:Sequencing of the Mycobacterium tuberculosis pncA gene allows for pyrazinamide susceptibility testing. We summarize data on pncA polymorphisms that do not confer resistance at a susceptibility breakpoint of 100 μg/ml pyrazinamide in MGIT within a cohort of isolates from South Africa and the U.S. Centers for Disease Co...

journal_title：Journal of clinical microbiology

authors： Whitfield MG,Warren RM,Streicher EM,Sampson SL,Sirgel FA,van Helden PD,Mercante A,Willby M,Hughes K,Birkness K,Morlock G,van Rie A,Posey JE

更新日期：2015-11-01 00:00:00

abstract：:To compare Simplexa Flu A/B & RSV PCR with cytospin-immunofluorescence and laboratory-developed TaqMan PCR methods, 445 nasopharyngeal samples were tested. Of these, 199 were positive (46 for respiratory syncytial virus [RSV], 120 for influenza A, and 33 for influenza B) and 246 were negative. The direct fluorescent-a...

journal_title：Journal of clinical microbiology

authors： Landry ML,Ferguson D

更新日期：2014-08-01 00:00:00

abstract：:Human herpesvirus 6 (HHV-6) has occasionally been associated with cases of interstitial pneumonitis, mainly in individuals with impaired cellular immunity. Here we report for the first time severe interstitial pneumonitis with simultaneous HHV-6 and Pneumocystis carinii infections in the lung tissue of a young patient...

journal_title：Journal of clinical microbiology

authors： Vuorinen T,Kotilainen P,Lautenschlager I,Kujari H,Krogerus L,Oksi J

更新日期：2004-11-01 00:00:00

abstract：:A procedure for detecting pneumococcal capsular polysaccharides in immune complexes is described. Separation of antigen from immune complexes is achieved by electrophoresis at 56 degrees C. ...

journal_title：Journal of clinical microbiology

authors： Coonrod JD,Leach RP

更新日期：1978-08-01 00:00:00

abstract：:A microhematocrit concentration method (MH) for immediate diagnosis of Chagas' disease during the acute stage or in congenital cases was standardized. Parasitemia as low as 1,000 parasites per ml was detected, after centrifugation of six 50-microliters capillary tubes, by 10-min microscopic observation of each buffy c...

journal_title：Journal of clinical microbiology

authors： Feilij H,Muller L,Gonzalez Cappa SM

更新日期：1983-08-01 00:00:00

abstract：:A matched case-control study was conducted in the Maternal and Child Health Clinic (MCH) in Ifakara, Tanzania, during the rainy season in order to elucidate the risk factors for and etiology of diarrheal diseases in children under 5 years of age. Cases (103) and controls (206) were matched for sex and age group. Preco...

journal_title：Journal of clinical microbiology

authors： Gascón J,Vargas M,Schellenberg D,Urassa H,Casals C,Kahigwa E,Aponte JJ,Mshinda H,Vila J

更新日期：2000-12-01 00:00:00

abstract：:Gram stains remain the cornerstone of diagnostic testing in the microbiology laboratory for the guidance of empirical treatment prior to availability of culture results. Incorrectly interpreted Gram stains may adversely impact patient care, and yet there are no comprehensive studies that have evaluated the reliability...

journal_title：Journal of clinical microbiology

authors： Samuel LP,Balada-Llasat JM,Harrington A,Cavagnolo R

更新日期：2016-06-01 00:00:00

abstract：:Among 452 samples that were positive by the Xpert MTB/RIF (Xpert) assay and MGIT 960 system (MGIT), 440 and 10 Mycobacterium tuberculosis samples were detected as rifampin susceptible and rifampin resistant, respectively. Two isolates that were rifampin susceptible by the MGIT system were rifampin resistant by the Xpe...

journal_title：Journal of clinical microbiology

authors： Mokaddas E,Ahmad S,Eldeen HS,Al-Mutairi N

更新日期：2015-04-01 00:00:00

abstract：:Clinical isolates of Haemophilus influenzae were assessed for the presence of fucK, hap, and sodC by hybridization with gene-specific probes, and isolates diverging from the expected H. influenzae genotype were characterized by phenotype and 16S rRNA gene sequencing. Two of 480 isolates were finally classified as vari...

journal_title：Journal of clinical microbiology

authors： Nørskov-Lauritsen N

更新日期：2009-08-01 00:00:00

abstract：:We retrospectively compared the utility of a fungal isolation device (Isolator) versus conventional techniques for recovering fungal organisms from blood cultures obtained from neutropenic cancer patients. Positive cultures were deemed true pathogens, possible pathogens, or contaminants according to laboratory and cli...

journal_title：Journal of clinical microbiology

authors： Creger RJ,Weeman KE,Jacobs MR,Morrissey A,Parker P,Fox RM,Lazarus HM

更新日期：1998-01-01 00:00:00

abstract：:There are several convenient and accurate molecular assays to detect respiratory bacterial infection. The NeoPlex RB-8 detection kit (NeoPlex RB-8) is a new multiplex real-time PCR assay that simultaneously detects Streptococcus pneumoniae, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, Haemo...

journal_title：Journal of clinical microbiology

authors： Kim JW,Hong SS,Lee IS,Chi HY,Kim SO,Kim HN,Hong SP

更新日期：2020-01-28 00:00:00

abstract：:mariPOC is a novel point-of-care test system for rapid detection of respiratory tract infections. We compared the performance of the mariPOC test to that of bacterial culture for detecting group A streptococcus (GAS) in 219 pharyngitis patients (ages 1-64 years) and 109 healthy asymptomatic controls (ages 19-69 years)...

journal_title：Journal of clinical microbiology

authors： Vakkila J,Koskinen JO,Brandt A,Muotiala A,Liukko V,Soittu S,Meriluoto S,Vesalainen M,Huovinen P,Irjala K

更新日期：2015-07-01 00:00:00

abstract：:The identification of pathogens in patients with bacterial keratitis remains problematic because standard diagnostic tests are negative for 40 to 60% of patients. A cross-sectional study was undertaken to determine if PCR and sequence analysis of 16S ribosomal DNA (rDNA) could be used to detect bacterial pathogens in ...

journal_title：Journal of clinical microbiology

authors： Knox CM,Cevellos V,Dean D

更新日期：1998-12-01 00:00:00

abstract：:Detection of human cytomegalovirus (CMV) DNA in clinical specimens is considered a cornerstone in the diagnosis of CMV disease. The aim of this study was to evaluate a newly designed LightCycler-based quantitative CMV PCR. Specimens of human origin (n = 200) were tested using the LightCycler PCR, the quantitative COBA...

journal_title：Journal of clinical microbiology

authors： Schaade L,Kockelkorn P,Ritter K,Kleines M

更新日期：2000-11-01 00:00:00

abstract：:Aspergillus terreus isolates collected from patients at The M. D. Anderson Cancer Center in Houston, TX, and at The University Hospital of Innsbruck, Austria, were analyzed using random amplification of polymorphic DNA-PCR with three different primers. No strain similarity in either institution was detected, indicatin...

journal_title：Journal of clinical microbiology

authors： Lass-Flörl C,Grif K,Kontoyiannis DP

更新日期：2007-08-01 00:00:00

abstract：:We compared the Difco ESP 384 blood culture system with the pediatric Septi-Chek system for the detection of bloodstream infections in pediatric patients. A total of 10,762 blood culture sets included an ESP aerobic bottle and a Septi-Chek bottle. From these cultures, a total of 278 organisms classified as probable pa...

journal_title：Journal of clinical microbiology

authors： Welby PL,Keller DS,Storch GA

更新日期：1995-05-01 00:00:00

abstract：:Data pertaining to 20 strains of Yensinia enterocolitica isolated from the cecal content of swine slaughtered at Toronto Abattoirs are presented. Fifteen of 20 cultures belonged to the two predominant human serotypes in Ontario (i.e., serotype O:3 and O:5,27.) Seven cultures of Y. enterocolitica O:3 belonged to the "C...

journal_title：Journal of clinical microbiology

authors： Toma S,Deidrick VR

更新日期：1975-12-01 00:00:00

abstract：:In a field trial conducted in cooperation with pediatricians in private practice, 198 nasopharyngeal swabs from children with suspected whooping cough were placed into charcoal-horse-blood transport medium with cephalexin (40 mg/liter). After preincubation at 36 degrees C for 1 to 2 days, the transport systems were ma...

journal_title：Journal of clinical microbiology

authors： Hoppe JE,Weiss A,Wörz S

更新日期：1988-06-01 00:00:00

abstract：:We report an unusual case of culture-proven pneumococcal and meningococcal mixed meningitis in an 18-month-old girl. The patient responded well to antimicrobial therapy and recovered completely without sequelae. No underlying condition could be demonstrated except a rhinitis of unknown etiology 2 days before the onset...

journal_title：Journal of clinical microbiology

authors： Marchandin H,Ventura V,Alonso JM,Van de Perre P

更新日期：2005-03-01 00:00:00

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) infection and colonization, involving MRSA strains which differ from common human health care-associated clones, have become serious emerging conditions in equine veterinary hospitals. In 2010, MRSA spa type t535 caused an outbreak involving both horses and personnel ...

journal_title：Journal of clinical microbiology

authors： Steinman A,Masarwa S,Tirosh-Levy S,Gleser D,Kelmer G,Adler A,Carmeli Y,Schwaber MJ

更新日期：2015-09-01 00:00:00

abstract：:In a prospective volume-controlled study, the sensitivity of the BACTEC MYCO/F LYTIC medium was similar to that of the Peds Plus/F and Plus Aerobic/F vials for detecting Brucella melitensis bacteremia, but the time to detection with the MYCO/F LYTIC medium (101.4 +/- 46.7 h) was significantly longer than that with the...

journal_title：Journal of clinical microbiology

authors： Yagupsky P

更新日期：2004-05-01 00:00:00

abstract：:We report on the performance of a recently introduced commercial chessboard method using 12 antisera, in comparison with that of the 55-antiserum panel used in determining the serogroups and types (SGTs) of Streptococcus pneumoniae, both of which were carried out by a coagglutination technique. Of a total of 150 strai...

journal_title：Journal of clinical microbiology

authors： Lalitha MK,Thomas K,Kumar RS,Steinhoff MC

更新日期：1999-01-01 00:00:00