Development of a molecular-beacon assay to detect the G1896A precore mutation in hepatitis B virus-infected individuals.

abstract：:We report a case of disseminated Scedosporium/Pseudallescheria infection due to Pseudallescheria boydii sensu stricto after lung transplantation in a patient with cystic fibrosis. Dissemination occurred under voriconazole. Despite surgery and combination therapy with voriconazole, caspofungin, and terbinafine, the pat...

journal_title：Journal of clinical microbiology

authors： Morio F,Horeau-Langlard D,Gay-Andrieu F,Talarmin JP,Haloun A,Treilhaud M,Despins P,Jossic F,Nourry L,Danner-Boucher I,Pattier S,Bouchara JP,Le Pape P,Miegeville M

更新日期：2010-05-01 00:00:00

abstract：:Actinobacillus pleuropneumoniae strains of serotypes 1 and 9 were studied for their serological properties by means of agglutination, coagglutination (CoA), indirect hemagglutination (IHA), Co-IHA, ring precipitation (RP), and immunodiffusion (ID) tests. Particulate and soluble antigens of unheated and heat-treated ba...

journal_title：Journal of clinical microbiology

authors： Mittal KR

更新日期：1990-03-01 00:00:00

abstract：:Tests commonly used for routine determination of anti-Toxoplasma gondii immunoglobulin G (IgG) antibodies show a high level of consistency. However, considerable variations between commercial screening tests are still observed in detecting antibodies present at low concentrations, leading to a number of discrepant and...

journal_title：Journal of clinical microbiology

authors： Franck J,Garin YJ,Dumon H

更新日期：2008-07-01 00:00:00

abstract：:mariPOC is a novel point-of-care test system for rapid detection of respiratory tract infections. We compared the performance of the mariPOC test to that of bacterial culture for detecting group A streptococcus (GAS) in 219 pharyngitis patients (ages 1-64 years) and 109 healthy asymptomatic controls (ages 19-69 years)...

journal_title：Journal of clinical microbiology

authors： Vakkila J,Koskinen JO,Brandt A,Muotiala A,Liukko V,Soittu S,Meriluoto S,Vesalainen M,Huovinen P,Irjala K

更新日期：2015-07-01 00:00:00

abstract：:We describe the serum immunoglobulin A (IgA) antibody response to Norwalk virus infection in human volunteers and compare it with previously described IgM and total antibody responses. Whereas specific IgA and IgM peak within 2 weeks after onset of symptoms, titers of total blocking antibody continue to rise, implying...

journal_title：Journal of clinical microbiology

authors： Erdman DD,Gary GW,Anderson LJ

更新日期：1989-06-01 00:00:00

abstract：:Clinically important species of Candida and related organisms were compared for extent of nucleotide divergence in the 5' end of the large-subunit (26S) ribosomal DNA (rDNA) gene. This rDNA region is sufficiently variable to allow reliable separation of all known clinically significant yeast species. Of the 204 descri...

journal_title：Journal of clinical microbiology

authors： Kurtzman CP,Robnett CJ

更新日期：1997-05-01 00:00:00

abstract：:The Kodak SureCell Herpes Test Kit (SC-HSV) was evaluated in a high-risk sexually transmitted disease clinic population. A total of 97 lesion specimens (from 94 patients) were tested for the presence of herpes simplex virus by SC-HSV and by spin-amplified tissue culture confirmed by an enzyme immunoassay. The overall ...

journal_title：Journal of clinical microbiology

authors： Dorian KJ,Beatty E,Atterbury KE

更新日期：1990-09-01 00:00:00

abstract：:Seventy-nine methicillin-resistant Staphylococcus aureus (MRSA) strains, isolated during 1980 to 1990, were classified as MRSA Aggl- (14 strains) and MRSA Aggl+ (65 strains) strains on the basis of test results in slide agglutination assays designed to detect fibrinogen-binding protein (clumping factor) and protein A ...

journal_title：Journal of clinical microbiology

authors： Kuusela P,Hildén P,Savolainen K,Vuento M,Lyytikäinen O,Vuopio-Varkila J

更新日期：1994-01-01 00:00:00

abstract：:The nucleotide sequences of the internal transcribed spacer (ITS) 1 and 2 regions in the rRNA gene were determined by directly sequencing PCR-amplified fragments for all of the species (17 species and five varieties) in the genus Trichosporon. Comparative sequence analysis suggests that six medically relevant species,...

journal_title：Journal of clinical microbiology

authors： Sugita T,Nishikawa A,Ikeda R,Shinoda T

更新日期：1999-06-01 00:00:00

abstract：:During a 1-year survey of Shiga toxin-producing Escherichia coli (STEC) prevalence in central France, 2,143 samples were investigated by PCR for Shiga toxin-encoding genes. A total of 330 (70%) of 471 fecal samples collected from healthy cattle at the Clermont-Ferrand slaughterhouse, 47 (11%) of 411 beef samples, 60 (...

journal_title：Journal of clinical microbiology

authors： Pradel N,Livrelli V,De Champs C,Palcoux JB,Reynaud A,Scheutz F,Sirot J,Joly B,Forestier C

更新日期：2000-03-01 00:00:00

abstract：:Rodent models have been developed to study the pathogenesis of diseases caused by Helicobacter pylori, as well as by other gastric and intestinal Helicobacter spp., but some murine enteric Helicobacter spp. cause hepatobiliary and intestinal tract diseases in specific inbred strains of laboratory mice. To identify the...

journal_title：Journal of clinical microbiology

authors： Nilsson HO,Ouis IS,Stenram U,Ljungh A,Moran AP,Wadström T,Al-Soud WA

更新日期：2004-08-01 00:00:00

abstract：:The increasing risk of Rift Valley fever virus (RVFV) infection as a global veterinary and public health threat demands the development of safe and accurate diagnostic tests. The aim of this study was to assess the suitability of a baculovirus expression system to produce recombinant RVFV nucleoprotein (N) for use as ...

journal_title：Journal of clinical microbiology

authors： Faburay B,Wilson WC,Secka A,Drolet B,McVey DS,Richt JA

更新日期：2019-09-24 00:00:00

abstract：:A clustered regularly interspaced short palindromic repeat (CRISPR) typing method has recently been developed and used for typing and subtyping of Salmonella spp., but it is complicated and labor intensive because it has to analyze all spacers in two CRISPR loci. Here, we developed a more convenient and efficient meth...

journal_title：Journal of clinical microbiology

authors： Li H,Li P,Xie J,Yi S,Yang C,Wang J,Sun J,Liu N,Wang X,Wu Z,Wang L,Hao R,Wang Y,Jia L,Li K,Qiu S,Song H

更新日期：2014-08-01 00:00:00

abstract：:Invasive aspergillosis (IA) is a life-threatening infection that mainly occurs in immunocompromised patients. Here, we compared the novel Aspergillus-specific galactomannoprotein (GP) enzyme-linked immunosorbent assay (ELISA) (Euroimmun Medizinische Labordiagnostika AG) to the established Platelia Aspergillus galactom...

journal_title：Journal of clinical microbiology

authors： Dichtl K,Seybold U,Ormanns S,Horns H,Wagener J

更新日期：2019-06-25 00:00:00

abstract：:Spirochetes of the genus Treponema were cultured from 7 of 10 cases of digital dermatitis in sheep. Two cultures comprised Treponema phagedenis-like and Treponema medium/Treponema vincentii-like spirochetes, respectively, while the remaining cultures comprised mixed populations of Treponema medium/Treponema vincentii-...

journal_title：Journal of clinical microbiology

authors： Sayers G,Marques PX,Evans NJ,O'Grady L,Doherty ML,Carter SD,Nally JE

更新日期：2009-04-01 00:00:00

abstract：:We evaluated the Phoenix automated microbiology system (BD Diagnostic Systems, Sparks, MD) for the identification (ID) and antimicrobial susceptibility testing (AST) of challenge and clinical staphylococci and enterococci recovered from patients in a tertiary-care medical center. In total, 424 isolates were tested: 90...

journal_title：Journal of clinical microbiology

authors： Carroll KC,Borek AP,Burger C,Glanz B,Bhally H,Henciak S,Flayhart DC

更新日期：2006-06-01 00:00:00

abstract：:Rotavirus is the most common cause of severe dehydrating gastroenteritis in infants. To date, 10 different serotypes of rotavirus have been identified in human stools. While four or five serotypes dominate, serotype circulation varies with season and geography. Since our laboratory has been involved in the development...

journal_title：Journal of clinical microbiology

authors： DiStefano DJ,Kraiouchkine N,Mallette L,Maliga M,Kulnis G,Keller PM,Clark HF,Shaw AR

更新日期：2005-12-01 00:00:00

abstract：:Performance in proficiency testing (PT) schemes is an objective measure of a laboratory's best performance. We examined the performance of participants in two parasitology PT schemes in South Africa from 2004 through 2010. The average rates of acceptable scores over the period were 58% and 66% for the stool and blood ...

journal_title：Journal of clinical microbiology

authors： Poonsamy B,Dini L,Frean J

更新日期：2012-10-01 00:00:00

abstract：:We investigated the presence of Chlamydia pneumoniae in 81 normal and pathological specimens obtained from postmortem brain tissues of patients with multiple sclerosis and with other neurological or nonneurological diseases. The assays used included PCR amplification of all DNA samples in the initial study. Culture an...

journal_title：Journal of clinical microbiology

authors： Hammerschlag MR,Ke Z,Lu F,Roblin P,Boman J,Kalman B

更新日期：2000-11-01 00:00:00

abstract：:Ureaplasma urealyticum is a commensal organism of the lower genital tract of females, but in a subpopulation of individuals, it can invade the upper genital tract. It is a significant cause of chorioamnionitis and neonatal morbidity and mortality. There are 14 recognized serovars of U. urealyticum; these can be divide...

journal_title：Journal of clinical microbiology

authors： Teng LJ,Zheng X,Glass JI,Watson HL,Tsai J,Cassell GH

更新日期：1994-06-01 00:00:00

abstract：:The prevalence of the Epstein-Barr virus (EBV) Taiwan variant was investigated in the throat washing (TW) samples from patients with head and neck tumors, persons with nonmalignant diseases, and healthy adults in Taiwan. By using the EBV (BNLF-1 gene)-specific primers and PCR, the EBV latent membrane protein gene BNLF...

journal_title：Journal of clinical microbiology

authors： Jeng KC,Hsu CY,Liu MT,Chung TT,Liu ST

更新日期：1994-01-01 00:00:00

abstract：:The usefulness of filter paper for preservation of bacterial cells was shown by mixed-linker DNA fingerprint analysis of Mycobacterium tuberculosis isolates from 77 Brazilian patients. DNA fingerprints of samples spotted onto filter paper and conventional culture material were identical. Thus, filter paper specimens a...

journal_title：Journal of clinical microbiology

authors： Burger M,Raskin S,Brockelt SR,Amthor B,Geiss HK,Haas WH

更新日期：1998-02-01 00:00:00

abstract：:The establishment of an optimal noninvasive method for diagnosing invasive aspergillosis (IA) is needed to improve the management of this life-threatening infection in patients with hematological disorders, and a number of noninvasive tests for IA that target different fungal components, including galactomannan, (1-->...

journal_title：Journal of clinical microbiology

authors： Kawazu M,Kanda Y,Nannya Y,Aoki K,Kurokawa M,Chiba S,Motokura T,Hirai H,Ogawa S

更新日期：2004-06-01 00:00:00

abstract：:A loop-mediated isothermal amplification (LAMP) assay for open reading frame 1 (ORF1) of the glutamine synthetase gene of Neisseria gonorrhoeae was able to tolerate urea concentrations of ≤ 1.8 M, compared with a PCR assay that was functional at concentrations of <100 mM. The LAMP assay was as sensitive as the PCR ass...

journal_title：Journal of clinical microbiology

authors： Edwards T,Burke PA,Smalley HB,Gillies L,Hobbs G

更新日期：2014-06-01 00:00:00

abstract：:A urease-based enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of reagin antibodies in serum. Visuwell Reagin (ADI Diagnostics Inc., Rexdale, Ontario, Canada) is a non-treponemal screening test for the serodiagnosis of syphilis which has the benefits of large batch testing, automatabilit...

journal_title：Journal of clinical microbiology

authors： White TJ,Fuller SA

更新日期：1989-10-01 00:00:00

abstract：:We conducted a Linear Array test/retest analysis using cytologic specimens from 198 women. A total of 67.2% of samples had the same human papillomavirus (HPV) types detected in both tests (type-specific positive agreement was 83.3% overall [Kappa = 0.9] and 86.8% for carcinogenic types [Kappa = 0.92]). Discordance was...

journal_title：Journal of clinical microbiology

authors： Koshiol J,Dunn ST,Walker JL,Zuna RE,Schiffman M,Sherman ME,Gold MA,Allen RA,Zhang R,Wang SS,Wentzensen N

更新日期：2013-02-01 00:00:00

abstract：:Genetic relationships within ureaplasma serovars were investigated by pulsed-field gel electrophoresis (PFGE). One hundred thirteen Ureaplasma parvum isolates and 78 Ureaplasma urealyticum isolates were different from their ATCC serovar type strains and different within the same serovars. The organisms were geographic...

journal_title：Journal of clinical microbiology

authors： Xiao L,Crabb DM,Moser SA,Duffy LB,Glass JI,Paralanov V,Waites KB

更新日期：2011-09-01 00:00:00

abstract：:Three hundred thirty-one yeast and yeast-like isolates were cultivated on eosin methylene blue agar. While the sensitivity rate for Candida kefyr isolates producing a metallic green sheen was 81.8%, the high positive predictive value (100%) for yeast isolates with this phenotype belonging to C. kefyr suggests that the...

journal_title：Journal of clinical microbiology

authors： Munson EL,Troy DR,Weber JK,Messer SA,Pfaller MA

更新日期：2002-11-01 00:00:00

abstract：:Twelve Salmonella typhimurium strains resistant to broad-spectrum cephalosporins were isolated from cases of gastroenteritis during 1996 to 1998 in Russia, Hungary, and Greece. Resistance was due to the production of CTX-M-type extended-spectrum beta-lactamases encoded by similar 12-kb plasmids. By pulsed-field gel el...

journal_title：Journal of clinical microbiology

authors： Tassios PT,Gazouli M,Tzelepi E,Milch H,Kozlova N,Sidorenko S,Legakis NJ,Tzouvelekis LS

更新日期：1999-11-01 00:00:00

abstract：:Enterobacter cloacae strain G6809 with reduced susceptibility to carbapenems was identified from a patient in a long-term acute care hospital in Kentucky. G6809 belonged to sequence type (ST) 88 and carried two carbapenemase genes, bla(KPC-18) and bla(VIM-1). Whole-genome sequencing localized bla(KPC-18) to the chromo...

journal_title：Journal of clinical microbiology

authors： Thomson GK,Snyder JW,McElheny CL,Thomson KS,Doi Y

更新日期：2016-03-01 00:00:00