Coproduction of KPC-18 and VIM-1 Carbapenemases by Enterobacter cloacae: Implications for Newer β-Lactam-β-Lactamase Inhibitor Combinations.

abstract：:The actinomycete Rhodococcus equi is an important pathogen of horses and an emerging opportunistic pathogen of humans. Identification of R. equi by classical bacteriological techniques is sometimes difficult, and misclassification of an isolate is not uncommon. We report here on a specific PCR assay for the rapid and ...

journal_title：Journal of clinical microbiology

authors： Ladrón N,Fernández M,Agüero J,González Zörn B,Vázquez-Boland JA,Navas J

更新日期：2003-07-01 00:00:00

abstract：:Shiga toxin (Stx)-producing Escherichia coli (STEC) is the main cause of postdiarrheal hemolytic-uremic syndrome (HUS), a life-threatening clinical complication characterized by hemolytic anemia, thrombocytopenia, and acute renal failure that mainly affects children. A relevant feature of STEC strains is the productio...

journal_title：Journal of clinical microbiology

authors： Melli LJ,Zylberman V,Hiriart Y,Lauche CE,Baschkier A,Pardo R,Miliwebsky E,Chinen I,Rivas M,Goldbaum FA,Ugalde JE,Comerci DJ,Ciocchini AE

更新日期：2020-02-24 00:00:00

abstract：:The Binax NOW assay (Binax, Inc., Portland, Maine) and the BD Directigen EZ assay (Becton Dickinson and Company, Sparks, Md.), two new rapid immunoassays for detection of respiratory syncytial virus (RSV), as well as the BD Directigen RSV assay (DRSV) (Becton Dickinson and Company) and direct immunofluorescence staini...

journal_title：Journal of clinical microbiology

authors： Ohm-Smith MJ,Nassos PS,Haller BL

更新日期：2004-07-01 00:00:00

abstract：:The clinical, histological, and bacteriological findings in five cases of acute appendicitis caused by Haemophilus segnis are reported. This is the first documentation of appendicitis associated with this organism. ...

journal_title：Journal of clinical microbiology

authors： Welch WD,Southern PM Jr,Schneider NR

更新日期：1986-11-01 00:00:00

abstract：:A gas-liquid chromatographic method which requires no chloroform extraction of the split products has been investigated for the detection of hippurate hydrolysis by Campylobacter spp. This technique gave better reproducibility than other tests also used in this study and allows the routine use of the gas-liquid chroma...

journal_title：Journal of clinical microbiology

authors： Bär W,Fricke G

更新日期：1987-09-01 00:00:00

abstract：:Viridans streptococci (VS) are common etiologic agents of subacute infective endocarditis and are capable of causing a variety of pyogenic infections. Many species of VS are difficult to differentiate by phenotypic traits. An oligonucleotide array based on 16S-23S rRNA gene intergenic spacer (ITS) sequences was develo...

journal_title：Journal of clinical microbiology

authors： Chen CC,Teng LJ,Kaiung S,Chang TC

更新日期：2005-04-01 00:00:00

abstract：:The serological properties of six new pneumococcal capsular types are described. A table listing all 90 pneumococcal types and their cross-reactions is included. ...

journal_title：Journal of clinical microbiology

authors： Henrichsen J

更新日期：1995-10-01 00:00:00

abstract：:Escherichia hermannii was isolated in pure culture from a patient with acute purulent conjunctivitis after a minor ocular injury. This is the first report of E. hermannii isolated as the sole pathogen from an infected site without prior antibiotic exposure, confirming the pathogenic potential of the microorganism. ...

journal_title：Journal of clinical microbiology

authors： Poulou A,Dimitroulia E,Markou F,Tsakris A

更新日期：2008-11-01 00:00:00

abstract：:A species-specific molecular beacon real-time PCR assay was developed for rapid diagnosis of Exserohilum rostratum infection. As low as 100 fg of E. rostratum DNA can be reliably detected in the presence of 50 ng of human DNA, with a dynamic linear quantification range from 20 ng to 200 fg. ...

journal_title：Journal of clinical microbiology

authors： Zhao Y,Petraitiene R,Walsh TJ,Perlin DS

更新日期：2013-03-01 00:00:00

abstract：:The Alere i Influenza A&B assay is a newly developed rapid molecular assay which has the potential to generate results within 15 min from sample collection. In this study, we evaluated the Alere i Influenza A&B assay by using salvaged frozen respiratory specimens that were collected in viral transport medium from chil...

journal_title：Journal of clinical microbiology

authors： Bell JJ,Selvarangan R

更新日期：2014-11-01 00:00:00

abstract：:An assay to detect and sequence DNA from human cytomegalovirus (HCMV) immediate-early gene region 1 has been developed; it involves in vitro amplification by the polymerase chain reaction and direct solid-phase sequencing of the amplified material. Urine samples from 16 patients tested positive for HCMV DNA in both a ...

journal_title：Journal of clinical microbiology

authors： Brytting M,Wahlberg J,Lundeberg J,Wahren B,Uhlén M,Sundqvist VA

更新日期：1992-04-01 00:00:00

abstract：:The 30-micrograms cefprozil disk and the cephalosporin class (30-micrograms cephalothin) disk were compared for their abilities to predict cefprozil susceptibility by agar disk diffusion testing. High error (5.02% major and 14.11% minor) rates were encountered with the cephalothin disk and were most frequently observe...

journal_title：Journal of clinical microbiology

authors： Fung-Tomc J,Stickle T,Doyle C,Huczko E,Kessler RE

更新日期：1991-11-01 00:00:00

abstract：:The Xpert MTB/RIF (Xpert) assay permits rapid near-patient detection of Mycobacterium tuberculosis in sputum; however, the test sensitivity remains suboptimal in paucibacillary specimens that are negative for acid-fast bacilli using smear microscopy. Xpert testing includes dilution with sample reagent, and when proces...

journal_title：Journal of clinical microbiology

authors： Dharan NJ,Amisano D,Mboowa G,Ssengooba W,Blakemore R,Kubiak RW,Armstrong DT,Jones M,Manabe YC,Joloba ML,Ellner JJ,Dorman SE,Alland D

更新日期：2015-04-01 00:00:00

abstract：:Vulvovaginal candidiasis (VVC) remains a common cause of morbidity, with three-quarters of women affected during their lifetimes. Use of antibiotics is an acknowledged trigger for VVC, which adversely affects women's physical and emotional health. Knowledge of patterns of genital Candida species-level identification i...

journal_title：Journal of clinical microbiology

authors： Pirotta MV,Garland SM

更新日期：2006-09-01 00:00:00

abstract：:We report the recovery of Histoplasma capsulatum from blood specimens cultured for Mycobacterium sp. in BACTEC 13A radiometric medium. H. capsulatum was recovered from six of eight blood specimens submitted for mycobacterial cultures from five human immunodeficiency virus-positive individuals. Initial positive metabol...

journal_title：Journal of clinical microbiology

authors： Merz WG,Kodsy S,Merz CS

更新日期：1992-01-01 00:00:00

abstract：:Strong positive CAMP reactions were demonstrated by 121 Vibrio cholerae O139 and 504 El Tor isolates, and weak positive CAMP reactions were shown by 235 non-O1, non O139 isolates when these isolates were tested by a modified CAMP technique. Thirty-five classical biotype V. cholerae O1 isolates included in the tests we...

journal_title：Journal of clinical microbiology

authors： Lesmana M,Albert MJ,Subekti D,Richie E,Tjaniadi P,Walz SE,Lebron CI

更新日期：1996-04-01 00:00:00

abstract：:During measles outbreaks, it is important to be able to rapidly distinguish between measles cases and vaccine reactions to avoid unnecessary outbreak response measures such as case isolation and contact investigations. We have developed a real-time reverse transcription-PCR (RT-PCR) method specific for genotype A meas...

journal_title：Journal of clinical microbiology

authors： Roy F,Mendoza L,Hiebert J,McNall RJ,Bankamp B,Connolly S,Lüdde A,Friedrich N,Mankertz A,Rota PA,Severini A

更新日期：2017-03-01 00:00:00

abstract：:Invasive aspergillosis (IA) is a life-threatening infection that mainly occurs in immunocompromised patients. Here, we compared the novel Aspergillus-specific galactomannoprotein (GP) enzyme-linked immunosorbent assay (ELISA) (Euroimmun Medizinische Labordiagnostika AG) to the established Platelia Aspergillus galactom...

journal_title：Journal of clinical microbiology

authors： Dichtl K,Seybold U,Ormanns S,Horns H,Wagener J

更新日期：2019-06-25 00:00:00

abstract：:Two types of commercially available microdilution trays were inoculated with log-phase cultures, stationary-phase cultures, and direct suspensions of 18- to 24-h colonies, with essentially comparable results. Direct pick-up of colonies was also tested with two Prompt inoculation systems (3M Co.); they were found to be...

journal_title：Journal of clinical microbiology

authors： Barry AL,Badal RE,Hawkinson RW

更新日期：1983-09-01 00:00:00

abstract：:The kinetics of delta antigen (HDAg) and anti-delta antibody (anti-HD) was analyzed in 22 acute delta hepatitis infections (11 coinfections and 11 superinfections), with an enzyme immunoassay developed by Organon Teknika and with two commercially available assays: Deltassay, a test for HDAg from Noctech and Abbott ant...

journal_title：Journal of clinical microbiology

authors： Dubois F,Goudeau A

更新日期：1988-07-01 00:00:00

abstract：:Commercially prepared fluorescein-labeled antihuman antibodies were labed with 125I and used to compre specific herpes simplex virus antibody titers as determined by indirect fluorescent antibody and radioimmunoassay techniques. Total virus-specific immunoglobulin and virus-specific immunoglobulin G titers did not var...

journal_title：Journal of clinical microbiology

authors： Parkinson AJ,Kalmakoff J

更新日期：1976-06-01 00:00:00

abstract：:We studied the evolution of Vibrio cholerae O1 during the 1991 to 2000 cholera epidemic in Mexico by biochemical, serological, and molecular characterization of strains collected during this period. Strains were divided into toxigenic and nontoxigenic groups according to the presence or absence of genes encoding chole...

journal_title：Journal of clinical microbiology

authors： Lizárraga-Partida ML,Quilici ML

更新日期：2009-05-01 00:00:00

abstract：:A continuous African green monkey kidney cell line, designated BGM, was compared with primary cynomolgus monkey kidney cells and human embryonic lung cells for efficiency of enterovirus isolation. A selective enhanced sensitivity of BGM cells both in terms of isolation rate and speed of isolation was found for group B...

journal_title：Journal of clinical microbiology

authors： Menegus MA,Hollick GE

更新日期：1982-05-01 00:00:00

abstract：:Blastocystis hominis is now gaining acceptance as an agent of human intestinal disease. A case-control study of the cause of gastroenteritis in children less than 6 years old was conducted. A total of 500 stool specimens were examined by wet mount preparation, formalin-ether concentration, Sheather's sugar flotation t...

journal_title：Journal of clinical microbiology

authors： Nimri LF

更新日期：1993-10-01 00:00:00

abstract：:Hafnia alvei strains which possess the attachment-effacement gene (eaeA) may have clinical importance as new diarrhea-causing pathogens and should therefore be differentiated from other H. alvei strains. We characterized diarrheal H. alvei strains, which were positive in the PCR test for the eaeA gene, using biochemic...

journal_title：Journal of clinical microbiology

authors： Ridell J,Siitonen A,Paulin L,Lindroos O,Korkeala H,Albert MJ

更新日期：1995-09-01 00:00:00

abstract：:Growth curves of the yeast form of Paracoccidioides brasiliensis B-339 based on total and viable cell counts were determined. Crude culture filtrate antigens were obtained after 7, 10, 15, 20, 25, and 30 days of incubation. Different patterns of proteins were obtained by affinity chromatography on Sepharose 4B-immunog...

journal_title：Journal of clinical microbiology

authors： De Camargo Z,Unterkircher C,Campoy SP,Travassos LR

更新日期：1988-10-01 00:00:00

abstract：:Multidrug-resistant opportunistic pathogens have become endemic to the veterinary hospital environment. Escherichia coli isolates resistant to 12 antibiotics were isolated from two dogs that were housed in the intensive care unit at The University of Georgia Veterinary Teaching Hospital within 48 h of each other. Revi...

journal_title：Journal of clinical microbiology

authors： Sanchez S,McCrackin Stevenson MA,Hudson CR,Maier M,Buffington T,Dam Q,Maurer JJ

更新日期：2002-10-01 00:00:00

abstract：:"Campylobacter pyloridis" has been recently described as a gastritis-associated bacterium. We studied 20 strains. The bacteria had most of the characteristics of Campylobacter spp. strains. They were hippurate negative and tolerant to triphenyl tetrazolium chloride (at 0.4 and 1 mg/ml). They grew on all the media comm...

journal_title：Journal of clinical microbiology

authors： Megraud F,Bonnet F,Garnier M,Lamouliatte H

更新日期：1985-12-01 00:00:00

abstract：:Herpes simplex virus type 1 (HSV-1) encodes 11 envelope glycoproteins, of which glycoprotein G-1 (gG-1) induces a type-specific antibody response. Variability of the gG-1 gene among wild-type strains may be a factor of importance for a reliable serodiagnosis and typing of HSV-1 isolates. Here, we used a gG-1 type-spec...

journal_title：Journal of clinical microbiology

authors： Rekabdar E,Tunbäck P,Liljeqvist JA,Lindh M,Bergström T

更新日期：2002-09-01 00:00:00

abstract：:Antibiogram typing of methicillin-resistant Staphylococcus aureus with selected antibiotics was evaluated as a primary epidemiological typing tool and compared with ribotyping. Antibiograms were derived with the Kirby-Bauer disk diffusion method by using erythromycin, clindamycin, cotrimoxazole, gentamicin, and ciprof...

journal_title：Journal of clinical microbiology

authors： Blanc DS,Lugeon C,Wenger A,Siegrist HH,Francioli P

更新日期：1994-10-01 00:00:00