Simple differentiation of Vibrio cholerae O139 from V. cholerae O1 and non-O1, non-O139 by modified CAMP test.

abstract：:Pneumocystis jirovecii pneumonia (PcP) is a major cause of respiratory illness in patients with AIDS. The identification of multiple single-nucleotide polymorphisms (SNPs) at three distinct P. jirovecii loci encoding dihydrofolate reductase (DHFR), mitochondrial large-subunit rRNA (mtLSU rRNA), and superoxide dismutas...

journal_title：Journal of clinical microbiology

authors： Esteves F,Gaspar J,De Sousa B,Antunes F,Mansinho K,Matos O

更新日期：2011-05-01 00:00:00

abstract：:Rapid and accurate identification of mycobacterial species is essential for patient management. We describe the use of the Invader assay in conjunction with the BACTEC MGIT 960 system that together provide an efficient procedure for clinical use. This assay discriminates single-base differences (e.g., genotyping singl...

journal_title：Journal of clinical microbiology

authors： Ichimura S,Nagano M,Ito N,Shimojima M,Egashira T,Miyamoto C,Ohkusu K,Ezaki T

更新日期：2007-10-01 00:00:00

abstract：:Global HIV treatment programs need sensitive and affordable tests to monitor HIV drug resistance. We compared mutant detection by the oligonucleotide ligation assay (OLA), an economical and simple test, to massively parallel sequencing. Nonnucleoside reverse transcriptase inhibitor (K103N, V106M, Y181C, and G190A) and...

journal_title：Journal of clinical microbiology

authors： Beck IA,Deng W,Payant R,Hall R,Bumgarner RE,Mullins JI,Frenkel LM

更新日期：2014-07-01 00:00:00

abstract：:We previously demonstrated that whole blood contains significantly more hepatitis C virus (HCV) RNA than plasma. To validate the whole-blood-based HCV RNA detection method, a prospective comparison of HCV RNA detection in whole blood and plasma from 50 patients with chronic liver disease was undertaken. Whole-blood an...

journal_title：Journal of clinical microbiology

authors： Stapleton JT,Klinzman D,Schmidt WN,Pfaller MA,Wu P,LaBrecque DR,Han Jq,Phillips MJ,Woolson R,Alden B

更新日期：1999-03-01 00:00:00

abstract：:The replication of human coronavirus strain 229E was observed by using indirect immunofluorescence in infected monolayers of MRC continuous cells. By 8 h after infection, bright cytoplasmic fluorescence was detected in cells infected with human coronavirus 229E. Discrete foci of infection were observed from 8 to 16 h ...

journal_title：Journal of clinical microbiology

authors： Macnaughton MR,Thomas BJ,Davies HA,Patterson S

更新日期：1980-09-01 00:00:00

abstract：:Ribotyping was compared with multilocus enzyme electrophoresis (MEE) for subtyping 305 Listeria monocytogenes isolates from clinical and nonclinical sources. For ribotyping, EcoRI-restricted genomic DNA fragments of L. monocytogenes strains were separated by agarose gel electrophoresis, and Southern blots were probed ...

journal_title：Journal of clinical microbiology

authors： Graves LM,Swaminathan B,Reeves MW,Hunter SB,Weaver RE,Plikaytis BD,Schuchat A

更新日期：1994-12-01 00:00:00

abstract：:One hundred ninety-three Streptococcus agalactiae isolates of neonatal origin and 146 isolates from adult women were analyzed for macrolide resistance and investigated for clonality. Among erythromycin-resistant isolates, serotype V turned out to be the most frequent. Comparative pulsed-field gel electrophoresis analy...

journal_title：Journal of clinical microbiology

authors： von Both U,Ruess M,Mueller U,Fluegge K,Sander A,Berner R

更新日期：2003-05-01 00:00:00

abstract：:Blastocystis hominis is now gaining acceptance as an agent of human intestinal disease. A case-control study of the cause of gastroenteritis in children less than 6 years old was conducted. A total of 500 stool specimens were examined by wet mount preparation, formalin-ether concentration, Sheather's sugar flotation t...

journal_title：Journal of clinical microbiology

authors： Nimri LF

更新日期：1993-10-01 00:00:00

abstract：:The aim of this study was to develop a PCR and reverse line blot hybridization (PCR-RLB) macroarray assay based on 16S-23S rRNA gene internal transcribed spacer sequences for the identification and differentiation of 34 mycobacterial species or subspecies. The performance of the PCR-RLB assay was assessed and validate...

journal_title：Journal of clinical microbiology

authors： Xiong L,Kong F,Yang Y,Cheng J,Gilbert GL

更新日期：2006-10-01 00:00:00

abstract：:The pathogenesis of gastroduodenal diseases is related to the diversity of Helicobacter pylori strains. CagA-positive strains are more likely to cause gastric cancer than CagA-negative strains. Based on EPIYA (Glu-Pro-Ile-Tyr-Ala) motifs at the carboxyl terminus corresponding to phosphorylation sites, H. pylori CagA i...

journal_title：Journal of clinical microbiology

authors： Truong BX,Mai VT,Tanaka H,Ly le T,Thong TM,Hai HH,Van Long D,Furumatsu K,Yoshida M,Kutsumi H,Azuma T

更新日期：2009-12-01 00:00:00

abstract：:A duplex PCR to detect Bordetella pertussis and Bordetella parapertussis was developed with the insertion sequences IS481 (B. pertussis) and IS1001 (B. parapertussis) and evaluated with specimens from 520 consecutive patients presenting with possible pertussis. No culture-positive-PCR-negative results occurred, giving...

journal_title：Journal of clinical microbiology

authors： Farrell DJ,Daggard G,Mukkur TK

更新日期：1999-03-01 00:00:00

abstract：:In 1998, an outbreak of systemic infections caused by Bacillus cereus occurred in the Neonatal Intensive Care Unit of the University Hospital Vrije Universiteit, Amsterdam, The Netherlands. Three neonates developed sepsis with positive blood cultures. One neonate died, and the other two neonates recovered. An environm...

journal_title：Journal of clinical microbiology

authors： Van Der Zwet WC,Parlevliet GA,Savelkoul PH,Stoof J,Kaiser AM,Van Furth AM,Vandenbroucke-Grauls CM

更新日期：2000-11-01 00:00:00

abstract：:We report a case of Coccidioides thyroiditis in an HIV-infected patient with a history of recent Coccidioides pneumonia but with negative Coccidioides serology determined by enzyme immunoassay at presentation. Diagnosis of Coccidioides thyroiditis was made based on histopathologic examination and culture of thyroid ab...

journal_title：Journal of clinical microbiology

authors： Jinno S,Chang S,Jacobs MR

更新日期：2012-07-01 00:00:00

abstract：:To study whether pathogenic clusters of Borrelia burgdorferi sensu lato strains occur, we typed 136 isolates, cultured from specimens from patients (n = 49) with various clinical entities and from ticks (n = 83) or dogs (n = 4) from different geographic regions, by randomly amplified polymorphic DNA (RAPD) fingerprint...

journal_title：Journal of clinical microbiology

authors： Wang G,van Dam AP,Spanjaard L,Dankert J

更新日期：1998-03-01 00:00:00

abstract：:A rapid, sensitive counterimmunoelectrophoresis assay was developed to detect adenovirus in stools of patients with gastroenteritis. The parameters of the assay were optimized, and its sensitivity and efficiency was examined. The assay promises to be a useful alternative to conventional techniques of cell culture isol...

journal_title：Journal of clinical microbiology

authors： Mankikar SD,Middleton PJ,Petric M

更新日期：1979-08-01 00:00:00

abstract：:Candida parapsilosis is an increasing cause of bloodstream infections (BSIs) in neonatal intensive care units (NICUs). It has been a persistent problem in the NICU of Hospital for Children and Adolescents, Helsinki University Central Hospital, Helsinki, Finland, since 1987. Fluconazole prophylaxis has been used to con...

journal_title：Journal of clinical microbiology

authors： Sarvikivi E,Lyytikäinen O,Soll DR,Pujol C,Pfaller MA,Richardson M,Koukila-Kähkölä P,Luukkainen P,Saxén H

更新日期：2005-06-01 00:00:00

abstract：:The peripheral blood lymphocytes from 48 heparinized blood specimens from human immunodeficiency virus (HIV) antibody-positive individuals were divided into two aliquots. One aliquot was reconstituted in one of the following five media. Medium 1 consisted of tryptose broth with 0.5% gelatin; medium 2 consisted of RPMI...

journal_title：Journal of clinical microbiology

authors： Gallo D,Kimpton JS,Johnson PJ

更新日期：1989-01-01 00:00:00

abstract：:Molecular techniques are playing an important role in the diagnosis of nontuberculous mycobacterial infections. This case report describes a chronic soft tissue infection in an immunocompetent patient caused by a previously undescribed pigmented, rapidly growing Mycobacterium species, emphasizing the importance of cli...

journal_title：Journal of clinical microbiology

authors： Turenne CY,Suchak AA,Wolfe JN,Kabani A,Nicolle LE

更新日期：2003-06-01 00:00:00

abstract：:Methicillin-resistant coagulase-negative staphylococci were isolated from the nares and skin of 1- to 8-week-old healthy chickens in three flocks from a farm. Isolation of methicillin-resistant coagulase-negative staphylococci was positive for 72 (25.7%) of the 280 chickens tested, with the frequency varying from 2.2 ...

journal_title：Journal of clinical microbiology

authors： Kawano J,Shimizu A,Saitoh Y,Yagi M,Saito T,Okamoto R

更新日期：1996-09-01 00:00:00

abstract：:We used the slide agglutination test to determine the serologic relationships of 22 Legionella spp. representing 33 serogroups. Antisera prepared against 14 of the Legionella spp. contained cross-reactive antibodies (1+ or greater) at their working dilutions. Numerous cross-reactions were observed for the blue-white f...

journal_title：Journal of clinical microbiology

authors： Thacker WL,Plikaytis BB,Wilkinson HW

更新日期：1985-05-01 00:00:00

abstract：:Members of the genus Exophiala are often difficult to identify to the species level because of their variable morphological appearances. This paper describes the methods used to identify Exophiala mesophila and provides salient differential features for distinguishing other mesophilic members of the genus. ...

journal_title：Journal of clinical microbiology

authors： Porteous NB,Grooters AM,Redding SW,Thompson EH,Rinaldi MG,De Hoog GS,Sutton DA

更新日期：2003-08-01 00:00:00

abstract：:Rotavirus is the most common cause of severe dehydrating gastroenteritis in infants. To date, 10 different serotypes of rotavirus have been identified in human stools. While four or five serotypes dominate, serotype circulation varies with season and geography. Since our laboratory has been involved in the development...

journal_title：Journal of clinical microbiology

authors： DiStefano DJ,Kraiouchkine N,Mallette L,Maliga M,Kulnis G,Keller PM,Clark HF,Shaw AR

更新日期：2005-12-01 00:00:00

abstract：:The QuantiFERON-TB Gold Plus (QFT-Plus; Qiagen, Germantown, MD) interferon gamma release assay (IGRA) received FDA clearance in 2017 and will replace the prior version of the assay, the QFT-Gold In-Tube (QFT-GIT). Here, we compared performances of the QFT-Plus assay and the QFT-GIT version in a diverse patient populat...

journal_title：Journal of clinical microbiology

authors： Theel ES,Hilgart H,Breen-Lyles M,McCoy K,Flury R,Breeher LE,Wilson J,Sia IG,Whitaker JA,Clain J,Aksamit TR,Escalante P

更新日期：2018-06-25 00:00:00

abstract：:Characteristics differentiating Escherichia coli strains that cause cystitis or pyelonephritis from fecal E. coli remain incompletely defined, particularly among adult women in the United States. Accordingly, phylogenetic group, O antigens, and virulence factors (VFs) were analyzed among 329 E. coli isolates from the ...

journal_title：Journal of clinical microbiology

authors： Johnson JR,Owens K,Gajewski A,Kuskowski MA

更新日期：2005-12-01 00:00:00

abstract：:Evaluations of screening tests for bacteriuria have traditionally compared the test results with those of quantitative urine cultures. However, many patients with symptomatic urinary tract infections can have less than 10(5) CFU/ml in their urine. Therefore, the results of urine culture and three screening tests (Bac-...

journal_title：Journal of clinical microbiology

authors： Murray PR,Smith TB,McKinney TC Jr

更新日期：1987-03-01 00:00:00

abstract：:This study was undertaken to develop an accurate, yet inexpensive, method for determining whether the bowel of a neutropenic patient is colonized with bacteria resistant to the antimicrobial agents used in empiric therapy. Selective agar media were prepared in which Mueller-Hinton agar or MacConkey agar were supplemen...

journal_title：Journal of clinical microbiology

authors： Smith JA,Sherlock CH,Burdge DR

更新日期：1984-12-01 00:00:00

abstract：:A species-specific molecular beacon real-time PCR assay was developed for rapid diagnosis of Exserohilum rostratum infection. As low as 100 fg of E. rostratum DNA can be reliably detected in the presence of 50 ng of human DNA, with a dynamic linear quantification range from 20 ng to 200 fg. ...

journal_title：Journal of clinical microbiology

authors： Zhao Y,Petraitiene R,Walsh TJ,Perlin DS

更新日期：2013-03-01 00:00:00

abstract：:Multidrug-resistant tuberculosis is an increasing health problem worldwide, especially in developing countries. The PCR-UHG-Rif assay, which detects mutations within the rpoB gene associated with rifampin resistance, was evaluated for its ability and reliability to detect and identify drug-resistant Mycobacterium tube...

journal_title：Journal of clinical microbiology

authors： Mayta H,Gilman RH,Arenas F,Valencia T,Caviedes L,Montenegro SH,Ticona E,Ortiz J,Chumpitaz R,Evans CA,Williams DL,Tuberculosis Working Group in Perú.

更新日期：2003-12-01 00:00:00

abstract：:Combination therapy can successfully suppress human immunodeficiency virus (HIV) replication in patients but selects for drug resistance, requiring subsequent resistance-guided therapeutic changes. This report describes the development and validation of a novel assay that offers a uniform method to measure susceptibil...

journal_title：Journal of clinical microbiology

authors： Covens K,Dekeersmaeker N,Schrooten Y,Weber J,Schols D,Quiñones-Mateu ME,Vandamme AM,Van Laethem K

更新日期：2009-07-01 00:00:00

abstract：:We systematically evaluated 5 methods for testing daptomycin versus 48 Enterococcus faecalis, 51 Enterococcus faecium, and 50 Staphylococcus aureus isolates using (i and ii) broth microdilution (BMD) with 50-mg/liter calcium medium supplementation (reference method) and 30-mg/liter calcium medium supplementation (BMD3...

journal_title：Journal of clinical microbiology

authors： Kirn TJ,Onyeaso E,Syed M,Weinstein MP

更新日期：2014-06-01 00:00:00