Outbreak of Bacillus cereus infections in a neonatal intensive care unit traced to balloons used in manual ventilation.

abstract：:Acrophialophora fusispora is an emerging opportunistic fungus capable of causing human infections. The taxonomy of the genus is not yet resolved and, in order to facilitate identification of clinical specimens, we have studied a set of clinical and environmental Acrophialophora isolates by morphological and molecular ...

journal_title：Journal of clinical microbiology

authors： Sandoval-Denis M,Gené J,Sutton DA,Wiederhold NP,Guarro J

更新日期：2015-05-01 00:00:00

abstract：:The purpose of the present study was to determine the extent to which bacteria not detected by culture contribute to the microbial flora of the bowel of preterm infants with and without neonatal necrotizing enterocolitis (NEC). Fecal samples from 32 preterm infants in special care baby units including samples from 10 ...

journal_title：Journal of clinical microbiology

authors： Millar MR,Linton CJ,Cade A,Glancy D,Hall M,Jalal H

更新日期：1996-10-01 00:00:00

abstract：:Streptococcus pneumoniae is a major cause of bacteremia, meningitis, pneumonia, sinusitis, and acute otitis media in children. Although optochin susceptibility, bile solubility, and Quellung testing are the standards for identifying and differentiating pneumococci, there are several reports of nontypeable pneumococci ...

journal_title：Journal of clinical microbiology

authors： Ing J,Mason EO,Kaplan SL,Lamberth LB,Revell PA,Luna RA,Hulten KG

更新日期：2012-04-01 00:00:00

abstract：:The value of immunoglobulin M (IgM) detection in the early diagnosis of influenza A was examined in a prospective study during an outbreak of influenza A/Philippines/2/82 (H3N2) virus infection in February and March 1986. The IgM response was investigated in sera from 64 adults with influenzalike symptoms; we found a ...

journal_title：Journal of clinical microbiology

authors： Vikerfors T,Lindegren G,Grandien M,van der Logt J

更新日期：1989-03-01 00:00:00

abstract：:Commercial chemiluminescent DNA probes (Accuprobe; Gen-Probe, San Diego, Calif.) for the identification of Mycobacterium tuberculosis (MTB) complex, M. avium complex (MAC), M. gordonae, and M. kansasii were evaluated with 134 clinical isolates. These included 36 MTB complex, 40 MAC, 27 M. gordonae, 9 M. kansasii, and ...

journal_title：Journal of clinical microbiology

authors： Lebrun L,Espinasse F,Poveda JD,Vincent-Levy-Frebault V

更新日期：1992-09-01 00:00:00

abstract：:A direct and an indirect enzyme immunoassay (EIA) were evaluated against a standard of electron microscopy after direct ultracentrifugation of the specimen for their performances in detecting rotaviruses. The indirect EIA had variable background activity which influenced test specificity. The indirect EIA control (tes...

journal_title：Journal of clinical microbiology

authors： Hammond GW,Ahluwalia GS,Barker FG,Horsman G,Hazelton PR

更新日期：1982-07-01 00:00:00

abstract：:The clinical significance of viridans streptococci isolated from the blood cultures of 86 patients was determined. Isolates that were significant or suggestive of infection represented only 21% of the cases. Among 54 isolates for which the species was known, Streptococcus sanguis II was the most common. However, a sig...

journal_title：Journal of clinical microbiology

authors： Swenson FJ,Rubin SJ

更新日期：1982-04-01 00:00:00

abstract：:RotaTeq (RV5) is a widely used live attenuated pentavalent rotavirus (RV) vaccine. Although fecal shedding of RV vaccine strains persists for long time periods, it is unclear how each vaccine strain replicates in intestinal tissue and is excreted in stool. To examine this issue, we established RV5 genotype-specific re...

journal_title：Journal of clinical microbiology

authors： Higashimoto Y,Ihira M,Miyazaki Y,Kuboshiki A,Yoshinaga S,Hiramatsu H,Suzuki R,Miyata M,Miura H,Komoto S,Yukitake J,Taniguchi K,Kawamura Y,Yoshikawa T

更新日期：2018-05-25 00:00:00

abstract：:We compared the results of Gram staining and culture of cerebrospinal fluid to results obtained with a rapid PCR assay for the diagnosis of meningococcal meningitis in 281 cases of suspected bacterial meningitis. PCR had a sensitivity of 97% compared to a sensitivity of 55% for culture, and the PCR specificity was 99....

journal_title：Journal of clinical microbiology

authors： Richardson DC,Louie L,Louie M,Simor AE

更新日期：2003-08-01 00:00:00

abstract：:We implemented hospital information system (HIS) alerts to deter unnecessary test orders for ovum and parasite (O&P) exams and Clostridium difficile PCR. The HIS alerts decreased noncompliant O&P orders (orders after >72 h of hospitalization) from 49.8% to 30.9%, an overall decrease of 19%, and reduced noncompliant C....

journal_title：Journal of clinical microbiology

authors： Otto CC,Shuptar SL,Milord P,Essick CJ,Nevrekar R,Granovsky SL,Seo SK,Babady NE,Martin SC,Tang YW,Pessin MS

更新日期：2015-08-01 00:00:00

abstract：:Astrovirus infections were detected by enzyme immunoassay in 12 (5%) of 251 stool samples from children with gastroenteritis from Bogota, Colombia. In addition, astroviruses were detected by reverse transcription-PCR in 3 (10%) of 29 stool samples negative for other enteric pathogens collected in Caracas, Venezuela, f...

journal_title：Journal of clinical microbiology

authors： Medina SM,Gutierrez MF,Liprandi F,Ludert JE

更新日期：2000-09-01 00:00:00

abstract：:A neutralizing monoclonal antibody, B37:1, against a human rotavirus (B37) with a "super-short" RNA pattern was derived. The antibody gave serotype-specific reactions with a panel of standard rotaviruses and was reactive in an enzyme immunoassay. Epidemiological studies of this new human serotype should now be possibl...

journal_title：Journal of clinical microbiology

authors： Tursi JM,Albert MJ,Bishop RF

更新日期：1987-12-01 00:00:00

abstract：:Of a total of 1043 macrolide-resistant Streptococcus pneumoniae isolates collected from 24 countries as part of PROTEKT 1999-2000, 71 isolates tested positive for both the mef(A) and erm(B) genes. Of 69 isolates subjected to further molecular investigations, all were resistant to tetracycline, 63 (91.3%) were resistan...

journal_title：Journal of clinical microbiology

authors： Farrell DJ,Morrissey I,Bakker S,Morris L,Buckridge S,Felmingham D

更新日期：2004-02-01 00:00:00

abstract：:Bacterial contamination frequently interferes with successful recovery of the Lyme spirochete from cultures of tissue from Borrelia burgdorferi-infected humans, rodents, or ticks. We used 0.20- and 0.45-microns-pore-size syringe-tip filters to recover spirochetes from cultures contaminated with other bacteria. Low con...

journal_title：Journal of clinical microbiology

authors： Jobe DA,Callister SM,Schell RF

更新日期：1993-07-01 00:00:00

abstract：:This study evaluated a PCR method for the rapid detection of clinically significant genotypes of vancomycin-resistant enterococci (VRE) in nosocomial surveillance specimens. Detection of the vanA and vanB genes by multiplex PCR using 657 specimens that showed presumptive growth of VRE on bile esculin azide agar contai...

journal_title：Journal of clinical microbiology

authors： Jayaratne P,Rutherford C

更新日期：1999-06-01 00:00:00

abstract：:Whether targeted or universal decolonization strategies for the control of methicillin-resistant Staphylococcus aureus (MRSA) select for resistance to decolonizing agents is unresolved. The REDUCE-MRSA trial (ClinicalTrials registration no. NCT00980980) provided an opportunity to investigate this question. REDUCE-MRSA...

journal_title：Journal of clinical microbiology

authors： Hayden MK,Lolans K,Haffenreffer K,Avery TR,Kleinman K,Li H,Kaganov RE,Lankiewicz J,Moody J,Septimus E,Weinstein RA,Hickok J,Jernigan J,Perlin JB,Platt R,Huang SS

更新日期：2016-11-01 00:00:00

abstract：:Blood was cultured in two vacuum bottles containing Columbia broth. Filtered air was admitted to one bottle (aerobic); the unvented bottle was considered anaerobic. Cultures were incubated at 35 C until growth occurred or for at least 7 days. Of 744 organisms isolated, 50% were isolated from both bottles, 30% from the...

journal_title：Journal of clinical microbiology

authors： Blazevic DJ,Stemper JE,Matsen JM

更新日期：1975-02-01 00:00:00

abstract：:We validated a commercial enzyme-linked immunosorbent assay for the detection of anti-CagA antibodies in Brazilian patients with Helicobacter pylori infection. The test presented high sensitivity (97.4%) and specificity (88.9%) when employed in patients without gastric carcinoma. However, in gastric carcinoma patients...

journal_title：Journal of clinical microbiology

authors： Rocha AM,Rocha GA,Santos A,de Oliveira CA,Queiroz DM

更新日期：2003-01-01 00:00:00

abstract：:Escherichia coli isolates that cause detachment of cell monolayers during in vitro adherence assays (cell-detaching E. coli [CDEC]) were recently reported as a potential new group of enteropathogenic bacteria. In the present study, 269 E. coli isolates from feces of children 1 to 5 years of age were identified as CDEC...

journal_title：Journal of clinical microbiology

authors： Marques LR,Abe CM,Griffin PM,Gomes TA

更新日期：1995-10-01 00:00:00

abstract：:The NucliSENS easyMAG automated system was compared to the column-based Qiagen method for Epstein-Barr virus (EBV) or cytomegalovirus (CMV) DNA extraction from whole blood before viral load determination using the corresponding R-gene amplification kits. Both extraction techniques exhibited a total agreement of 81.3% ...

journal_title：Journal of clinical microbiology

authors： Pillet S,Bourlet T,Pozzetto B

更新日期：2009-11-01 00:00:00

abstract：:Recent studies suggest that a group of Chlamydia strains known as TWAR, which are now proposed to be a new species called Chlamydia pneumoniae, may be a frequent cause of respiratory disease in the United States and many other countries. Current serotesting methods do not allow rapid screening of large numbers of samp...

journal_title：Journal of clinical microbiology

authors： Ladany S,Black CM,Farshy CE,Ossewaarde JM,Barnes RC

更新日期：1989-12-01 00:00:00

abstract：:We evaluated the performance of the DR. TBDR/NTM IVD kit, which was designed to detect Mycobacterium tuberculosis, rifampin-resistant M. tuberculosis, and nontuberculous mycobacteria, for detecting 110 positive and 50 negative cultures in Mycobacterium Growth Indicator Tubes. The accuracy rate of this kit for identifi...

journal_title：Journal of clinical microbiology

authors： Lee MR,Cheng A,Huang YT,Liu CY,Chung KP,Wang HC,Liang SK,Liao CH,Yu CJ,Hsueh PR

更新日期：2012-10-01 00:00:00

abstract：:Levels of hepatitis B virus (HBV) DNA in the blood serve as an important marker in monitoring the disease progression and treatment efficacy of chronic HBV infection. Several commercial assays are available for accurate measurement of HBV genomic DNA, but many of them are hampered by relatively low sensitivity and lim...

journal_title：Journal of clinical microbiology

authors： Sum SS,Wong DK,Yuen MF,Yuan HJ,Yu J,Lai CL,Ho D,Zhang L

更新日期：2004-08-01 00:00:00

abstract：:The usefulness of filter paper for preservation of bacterial cells was shown by mixed-linker DNA fingerprint analysis of Mycobacterium tuberculosis isolates from 77 Brazilian patients. DNA fingerprints of samples spotted onto filter paper and conventional culture material were identical. Thus, filter paper specimens a...

journal_title：Journal of clinical microbiology

authors： Burger M,Raskin S,Brockelt SR,Amthor B,Geiss HK,Haas WH

更新日期：1998-02-01 00:00:00

abstract：:Four sources of error associated with virus-specific immunoglobulin M (IgM) determination by indirect enzyme-linked immunosorbent assay were recognized and analyzed. First, competitive inhibition due to specific IgG was demonstrated by experiments involving addition and subtraction of rubella-specific IgG. Second, the...

journal_title：Journal of clinical microbiology

authors： Champsaur H,Fattal-German M,Arranhado R

更新日期：1988-02-01 00:00:00

abstract：:A fundamental role for the endosymbiotic bacteria Wolbachia pipientis in the pathogenesis of Dirofilaria immitis infections has emerged in recent years. Diagnostic opportunities arising from this breakthrough have not yet been fully exploited. This study was aimed at developing conventional and real-time PCR assays to...

journal_title：Journal of clinical microbiology

authors： Turba ME,Zambon E,Zannoni A,Russo S,Gentilini F

更新日期：2012-08-01 00:00:00

abstract：:We evaluated quantitative real-time PCR to establish the diagnosis of rotavirus gastroenteritis in a high-disease-burden population in Malawi using enzyme immunoassay as the gold standard diagnostic test. In 146 children with acute gastroenteritis and 65 asymptomatic children, we defined a cutoff point in the threshol...

journal_title：Journal of clinical microbiology

authors： Bennett A,Bar-Zeev N,Jere KC,Tate JE,Parashar UD,Nakagomi O,Heyderman RS,French N,Iturriza-Gomara M,Cunliffe NA

更新日期：2015-06-01 00:00:00

abstract：:A PCR method was developed to identify and fingerprint Candida krusei isolates simply and rapidly. The primer pair Arno1 and Arno2 was designed to amplify the polymorphic species-specific repetitive sequence CKRS-1 (C. krusei repeated sequence 1) that we identified in the nontranscribed intergenic regions (IGRs) of rR...

journal_title：Journal of clinical microbiology

authors： Carlotti A,Chaib F,Couble A,Bourgeois N,Blanchard V,Villard J

更新日期：1997-06-01 00:00:00

abstract：:In the present study, a novel broad-range real-time PCR was developed for the rapid detection of human pathogenic fungi. The assay targets a part of the 28S large-subunit ribosomal RNA (rDNA) gene. We investigated its application for the most important human pathogenic fungal genera, including Aspergillus, Candida, Cr...

journal_title：Journal of clinical microbiology

authors： Vollmer T,Störmer M,Kleesiek K,Dreier J

更新日期：2008-06-01 00:00:00

abstract：:Mitochondrial cytochrome b (cyt b) genes of 42 strains representing 23 species of the genus Trichosporon were partially sequenced to determine their molecular phylogenetic relationships. Almost half of the 22 strains investigated (from 11 different species) contained introns in their sequences. Analysis of a 396-bp co...

journal_title：Journal of clinical microbiology

authors： Biswas SK,Wang L,Yokoyama K,Nishimura K

更新日期：2005-10-01 00:00:00