Enzyme immunoassay to determine exposure to Chlamydia pneumoniae (strain TWAR).

abstract：:In order to differentiate species within the Borrelia burgdorferi sensu lato complex, LightCyler PCR and melting-curve analysis of the amplicons of two genes with intraspecies variability, the p66 gene and the recA gene, were performed. It was demonstrated that nested LightCycler PCR amplification of p66 is more sensi...

journal_title：Journal of clinical microbiology

authors： Mommert S,Gutzmer R,Kapp A,Werfel T

更新日期：2001-07-01 00:00:00

abstract：:A series of clinical NDM-5-producing E. coli isolates obtained from two surveillance networks of carbapenem-producing Enterobacterales from 2018-2019, namely Switzerland (NARA) and Germany (SurvCARE), were analyzed. The 33 NDM-5-producing E. coli isolates were highly resistant to β-lactams including the novel ß-lactam...

journal_title：Journal of clinical microbiology

authors： Chakraborty T,Sadek M,Yao Y,Imirzalioglu C,Stephan R,Poirel L,Nordmann P

更新日期：2020-12-23 00:00:00

abstract：:Screening for Chlamydia trachomatis infection in men has traditionally been limited to men who present with urethral symptoms, thereby limiting the detection of asymptomatic chlamydia infection in men. In order to effectively screen both symptomatic and asymptomatic men, we evaluated a newly developed polymerase chain...

journal_title：Journal of clinical microbiology

authors： Jaschek G,Gaydos CA,Welsh LE,Quinn TC

更新日期：1993-05-01 00:00:00

abstract：:The Xpert MTB/RIF (Xpert) assay permits rapid near-patient detection of Mycobacterium tuberculosis in sputum; however, the test sensitivity remains suboptimal in paucibacillary specimens that are negative for acid-fast bacilli using smear microscopy. Xpert testing includes dilution with sample reagent, and when proces...

journal_title：Journal of clinical microbiology

authors： Dharan NJ,Amisano D,Mboowa G,Ssengooba W,Blakemore R,Kubiak RW,Armstrong DT,Jones M,Manabe YC,Joloba ML,Ellner JJ,Dorman SE,Alland D

更新日期：2015-04-01 00:00:00

abstract：:Analysis of a polymorphic microsatellite locus was applied to 85 Candida albicans strains from healthy individuals. Comparison with strains from nonhealthy individuals previously analyzed in our laboratory showed an overall similarity, suggesting that all commensal strains have the ability to develop as pathogens. ...

journal_title：Journal of clinical microbiology

authors： Dalle F,Dumont L,Franco N,Mesmacque D,Caillot D,Bonnin P,Moiroux C,Vagner O,Cuisenier B,Lizard S,Bonnin A

更新日期：2003-05-01 00:00:00

abstract：:The appearance and persistence of immunoglobulin M (IgM) and IgG antibodies in postnatal rubella infections were studied by employing a solid-phase radioimmunoassay test. Altogether, 222 serial serum specimens from 51 patients with acute rubella infection were tested. Both IgG and IgM antibodies developed rapidly and ...

journal_title：Journal of clinical microbiology

authors： Meurman OH

更新日期：1978-01-01 00:00:00

abstract：:Arbitrarily primed polymerase chain reaction (AP-PCR) was used to characterize Legionella pneumophila serogroup 1. Cells from a single colony could be subtyped by AP-PCR within a few hours. The discrimination between strains of L. pneumophila serogroup 1 by AP-PCR was equivalent to that by monoclonal antibody analysis...

journal_title：Journal of clinical microbiology

authors： Gomez-Lus P,Fields BS,Benson RF,Martin WT,O'Connor SP,Black CM

更新日期：1993-07-01 00:00:00

abstract：:It has been shown that some, but not all, Escherichia coli strains isolated from urine adhere, in vitro, to the surface of uroepithelial or vaginal cells. In the present study, 212 strains, isolated from urine of 212 infected patients, were tested for adhesion by using an in vitro human cell line assay. A variable deg...

journal_title：Journal of clinical microbiology

authors： Chabanon G,Hartley CL,Richmond MH

更新日期：1979-10-01 00:00:00

abstract：:Enteroviruses have been reported in encephalitis cases. However, clinical and epidemiological characteristics of enteroviruses in encephalitis are not fully established. We prospectively investigated 204 children with encephalitis over a period of 2 years (2009 to 2010) for enterovirus. Enterovirus was detected in 45 ...

journal_title：Journal of clinical microbiology

authors： Kumar A,Shukla D,Kumar R,Idris MZ,Misra UK,Dhole TN

更新日期：2012-11-01 00:00:00

abstract：:This study used a nested multiplex PCR method to detect three periodontal pathogens in subgingival plaque collected before treatment and at 2 and 6 months posttreatment from 107 patients with severe, generalized periodontitis. The proportions of the patients who harbored these bacteria before periodontal treatment wer...

journal_title：Journal of clinical microbiology

authors： Ready D,D'Aiuto F,Spratt DA,Suvan J,Tonetti MS,Wilson M

更新日期：2008-10-01 00:00:00

abstract：:The virulence plasmid of Escherichia coli O157 strain EDL933 carries a 10-kb putative virulence gene designated toxB. Little is known about the distribution of this gene among E. coli O157 strains or its presence in other enterohemorrhagic E. coli (EHEC) and enteropathogenic E. coli (EPEC) strains. We developed PCR an...

journal_title：Journal of clinical microbiology

authors： Tozzoli R,Caprioli A,Morabito S

更新日期：2005-08-01 00:00:00

abstract：:Hepatitis C virus (HCV) genotype 4 (HCV-4) infection is considered to be difficult to treat and has become increasingly prevalent in European countries, including The Netherlands. Using a molecular epidemiological approach, the present study investigates the genetic diversity and evolutionary origin of HCV-4 in Amster...

journal_title：Journal of clinical microbiology

authors： de Bruijne J,Schinkel J,Prins M,Koekkoek SM,Aronson SJ,van Ballegooijen MW,Reesink HW,Molenkamp R,van de Laar TJ

更新日期：2009-12-01 00:00:00

abstract：:Chronic infection with hepatitis B virus (HBV) is an important cause of cirrhosis and cancer of the liver. HBV is currently classified into eight genotypes, A to H. Accumulated evidence shows that the genotype influences both the clinical course of infection and the response to treatment. We describe a new method for ...

journal_title：Journal of clinical microbiology

authors： Malmström S,Berglin-Enquist I,Lindh M

更新日期：2010-04-01 00:00:00

abstract：:In a multicenter study, the consistency of international units expressed by five commercially available rubella virus immunoglobulin G kits was evaluated. The linearity and within-run and between-run precision were determined for each kit. All kits demonstrated good linearity and had within-run and between-run precisi...

journal_title：Journal of clinical microbiology

authors： Dimech W,Bettoli A,Eckert D,Francis B,Hamblin J,Kerr T,Ryan C,Skurrie I

更新日期：1992-03-01 00:00:00

abstract：:Several problems remain before molecular biology-based techniques, such as PCR, are widely accepted for the detection of infectious agents. Among the most formidable of these problems are the inability of the tests to distinguish between viable and nonviable organisms. We approached this problem by using the fact that...

journal_title：Journal of clinical microbiology

authors： Jou NT,Yoshimori RB,Mason GR,Louie JS,Liebling MR

更新日期：1997-05-01 00:00:00

abstract：:Three forms of DNA-based typing procedures for Aspergillus fumigatus isolates have been developed over the last five years. The procedures are random amplification of polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) detection, and Southern hybridizations with various repetitive sequence-based pr...

journal_title：Journal of clinical microbiology

authors： Anderson MJ,Gull K,Denning DW

更新日期：1996-01-01 00:00:00

abstract：:We investigated the performance of a double-antigen sandwich recombinant enzyme immunoassay (EIA; Abbott Laboratories, North Chicago, Ill.) and compared it with that of a synthetic-peptide-based EIA (Biochem Immunosystems, Montreal, Quebec, Canada) for the detection of human immunodeficiency type 1 (HIV-1) and HIV-2 a...

journal_title：Journal of clinical microbiology

authors： Galli RA,Castriciano S,Fearon M,Major C,Choi KW,Mahony J,Chernesky M

更新日期：1996-04-01 00:00:00

abstract：:During the 2009 H1N1 influenza A outbreak, 773 children were tested for influenza by direct fluorescent-antibody testing with PCR confirmation. Direct fluorescent-antibody testing has a specificity of 99.6% but a sensitivity of only 65.0%. Physicians should recognize diagnostic limitations of direct fluorescent-antibo...

journal_title：Journal of clinical microbiology

authors： Bakerman P,Balasuriya L,Fried O,Tellez D,Garcia-Filion P,Dalton H

更新日期：2011-10-01 00:00:00

abstract：:In specific-pathogen-free dogs experimentally infected with Borrelia burgdorferi by tick exposure, treatment with high doses of amoxicillin or doxycycline for 30 days diminished but failed to eliminate persistent infection. Although joint disease was prevented or cured in five of five amoxicillin- and five of six doxy...

journal_title：Journal of clinical microbiology

authors： Straubinger RK,Summers BA,Chang YF,Appel MJ

更新日期：1997-01-01 00:00:00

abstract：:By the use of enzyme immunoassay and RNA electrophoresis, rotavirus serotyping was performed with stool specimens from 605 children. Serotypes 1, 2, 3, and 4 accounted for 63, 9, 9, and 19% of the cases, respectively, with considerable yearly variation. An average of three serotypes cocirculated each year. ...

journal_title：Journal of clinical microbiology

authors： Begue RE,Dennehy PH,Huang J,Martin P

更新日期：1992-06-01 00:00:00

abstract：:The effects of Tween 80 supplementation of liquid culture medium on the formation of the superficial L1 layer of the Mycobacterium avium-Mycobacterium intracellulare complex (MAC) were examined by serological and scanning electron microscopic experiments. Specific antiserum to the glycopeptidolipids on the L1 layer of...

journal_title：Journal of clinical microbiology

authors： Masaki S,Sugimori G,Okamoto A,Imose J,Hayashi Y

更新日期：1991-07-01 00:00:00

abstract：:Restriction fragment length polymorphism (RFLP) analysis with probes derived from the insertion element IS6110, the direct repeat sequence, and the polymorphic GC-rich sequence (PGRS) and a PCR-based typing method called spacer oligonucleotide typing (spoligotyping) were used to strain type Mycobacterium bovis isolate...

journal_title：Journal of clinical microbiology

authors： Costello E,O'Grady D,Flynn O,O'Brien R,Rogers M,Quigley F,Egan J,Griffin J

更新日期：1999-10-01 00:00:00

abstract：:We investigated the presence of Chlamydia pneumoniae in 81 normal and pathological specimens obtained from postmortem brain tissues of patients with multiple sclerosis and with other neurological or nonneurological diseases. The assays used included PCR amplification of all DNA samples in the initial study. Culture an...

journal_title：Journal of clinical microbiology

authors： Hammerschlag MR,Ke Z,Lu F,Roblin P,Boman J,Kalman B

更新日期：2000-11-01 00:00:00

abstract：:The microflora associated with three dentoalveolar abscesses was determined by cultural and molecular methods. 16S rRNA genes were randomly amplified by means of conserved eubacterial primers and cloned. Restriction fragment length polymorphism analysis of the clones and amplified genes encoding 16S rRNA from the cult...

journal_title：Journal of clinical microbiology

authors： Dymock D,Weightman AJ,Scully C,Wade WG

更新日期：1996-03-01 00:00:00

abstract：:The Leishmania species cause a variety of human disease syndromes. Methods for diagnosis and species differentiation are insensitive and many require invasive sampling. Although quantitative PCR (qPCR) methods are reported for leishmania detection, no systematic method to quantify parasites and determine the species i...

journal_title：Journal of clinical microbiology

authors： Weirather JL,Jeronimo SM,Gautam S,Sundar S,Kang M,Kurtz MA,Haque R,Schriefer A,Talhari S,Carvalho EM,Donelson JE,Wilson ME

更新日期：2011-11-01 00:00:00

abstract：:G3 rotaviruses have been reported rarely in cattle, and none have been characterized. We report the first genomic characterization of a bovine G3 rotavirus, CP-1, which had been biologically characterized in vivo and shown to cause age-independent diarrhea. CP-1 was a G3 rotavirus as its VP7 had 92 to 96% deduced amin...

journal_title：Journal of clinical microbiology

authors： El-Attar L,Dhaliwal W,Iturriza-Gómara M,Bridger JC

更新日期：2002-03-01 00:00:00

abstract：:We compared 14 molecular assays for their ability to detect the Mycobacterium tuberculosis complex in bronchoalveolar lavage fluid samples. Three approaches were followed. First, by using DNA from Mycobacterium bovis BCG, we determined the detection limits of the assays using routine molecular methods. Second, in orde...

journal_title：Journal of clinical microbiology

authors： Akkerman OW,van der Werf TS,de Boer M,de Beer JL,Rahim Z,Rossen JW,van Soolingen D,Kerstjens HA,van der Zanden AG

更新日期：2013-11-01 00:00:00

abstract：:A sensitive and reproducible enzyme-linked immunosorbent assay (ELISA) is described for the detection of immunoglobulin M and antibodies with specifity for human cytomegalovirus (CMV) early (CMV-EA) and late (CMV-LA) antigens. The emphasis is on the production of high-quality CMV antigens, CMV-EA and CMV-LA separately...

journal_title：Journal of clinical microbiology

authors： Middeldorp JM,Jongsma J,ter Haar A,Schirm J,The TH

更新日期：1984-10-01 00:00:00

abstract：:Immune pressure on lentiviruses exerted by cytotoxic T lymphocytes (CTL) selects for virus CTL epitope mutations. Currently employed methods for monitoring emerging CTL epitope mutations rely on the labor-intensive and time-consuming techniques of virus population or clonal sequencing. Here we describe the development...

journal_title：Journal of clinical microbiology

authors： Peyerl FW,Barouch DH,Bazick HS,Manuel E,Letvin NL

更新日期：2005-09-01 00:00:00

abstract：:Sequence analysis of 58 multidrug-resistant Mycobacterium tuberculosis complex strains from Germany and 55 susceptible strains from a reference collection comprising major phylogenetic lineages confirmed that variations in Rv2629, 191A/C and 965C/T, are specific for genotypes Beijing and Ghana, respectively, but not i...

journal_title：Journal of clinical microbiology

authors： Homolka S,Köser C,Archer J,Rüsch-Gerdes S,Niemann S

更新日期：2009-01-01 00:00:00