Increased Efficiency of Group B Coxsackievirus Isolation from Clinical Specimens by Use of BGM Cells.

abstract：:An aerobically incubated, agar-based medium was developed for amino acid decarboxylase testing of Enterobacteriaceae family members by replica-plating methods. Results with the new medium agreed 97 to 99% with the reference broth method of Moeller, and no false-positive reactions were encountered. ...

journal_title：Journal of clinical microbiology

authors： Maccani JE

更新日期：1979-12-01 00:00:00

abstract：:Yersinia enterocolitica biotype 1, serotype O:21 was isolated from feces or rectal washings of three members of one family in northwestern Saskatchewan. The three isolates gave positive pathogenicity tests in guinea pigs with cultures grown at 22 degrees C as inoculum. All three cases showed clinical symptoms consiste...

journal_title：Journal of clinical microbiology

authors： Martin T,Kasian GF,Stead S

更新日期：1982-10-01 00:00:00

abstract：:Serologic methods are an important part of a clinical laboratory's portfolio of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) tests and are essential to the broader response to coronavirus infectious disease 2019 (COVID-19), including epidemiological studies and vaccine development. There are currently ...

journal_title：Journal of clinical microbiology

authors： Loeffelholz MJ

更新日期：2020-10-21 00:00:00

abstract：:Tests commonly used for routine determination of anti-Toxoplasma gondii immunoglobulin G (IgG) antibodies show a high level of consistency. However, considerable variations between commercial screening tests are still observed in detecting antibodies present at low concentrations, leading to a number of discrepant and...

journal_title：Journal of clinical microbiology

authors： Franck J,Garin YJ,Dumon H

更新日期：2008-07-01 00:00:00

abstract：:A case of pyogenic liver abscess (PLA) due to Rhodococcus equi in an immunocompetent individual was successfully treated by combining surgery and antibiotics. The R. equi-targeted antimicrobial agents erythromycin and rifampin were used only after surgical resection of the lesion and identification of the infective or...

journal_title：Journal of clinical microbiology

authors： Napoleão F,Damasco PV,Camello TC,do Vale MD,de Andrade AF,Hirata R Jr,de Mattos-Guaraldi AL

更新日期：2005-02-01 00:00:00

abstract：:Forty-five percent of 108 nontypable strains of Staphylococcus aureus from clinical and environmental sources were phage typable after heat shock and acridine orange treatment. Although phage patterns were produced by different types of treatment, the same phage groups were present, showing specificity. The newly typa...

journal_title：Journal of clinical microbiology

authors： Perkins RE,Kundsin RB

更新日期：1976-10-01 00:00:00

abstract：:Alveolar echinococcosis (AE), caused by the Echinococcus multilocularis metacestode, represents one of the most frequently fatal zoonoses. Early diagnosis significantly reduces morbidity and mortality associated with AE. Diagnosis of AE largely depends on a combination of imaging and serological tests due to its minim...

journal_title：Journal of clinical microbiology

authors： Ahn CS,Cai H,Kim JG,Han X,Ma X,Bae YA,Yang HJ,Kang I,Wang H,Kong Y

更新日期：2015-10-01 00:00:00

abstract：:The binding of human immunoglobulin G by Fc receptors in herpes simplex virus (HSV)-infected cells can cause false-positive interpretations in the immunofluorescence test for HSV antibody. When the infected cell smears were treated with 10% glacial acetic acid for 5 min and rinsed in phosphate-buffered saline before t...

journal_title：Journal of clinical microbiology

authors： Gallo D

更新日期：1986-10-01 00:00:00

abstract：:Global HIV treatment programs need sensitive and affordable tests to monitor HIV drug resistance. We compared mutant detection by the oligonucleotide ligation assay (OLA), an economical and simple test, to massively parallel sequencing. Nonnucleoside reverse transcriptase inhibitor (K103N, V106M, Y181C, and G190A) and...

journal_title：Journal of clinical microbiology

authors： Beck IA,Deng W,Payant R,Hall R,Bumgarner RE,Mullins JI,Frenkel LM

更新日期：2014-07-01 00:00:00

abstract：:To determine sensitivity and specificity of immunofluorescence assay (IFA) for detection of Helicobacter pylori, we studied 151 patients. Biopsies of gastric mucosae were obtained for culture, histological testing, and IFA. H. pylori serum antibodies were tested by enzyme-linked immunosorbent assay. IFA was done on Fo...

journal_title：Journal of clinical microbiology

authors： Rivera E,López-Vidal Y,Luqueño V,Ruiz-Palacios GM

更新日期：1991-08-01 00:00:00

abstract：:Chronic hepatitis B virus (HBV) infection via perinatal transmission is common in the Asia-Pacific region, but related quasispecies (QS) characteristics are not yet defined. To investigate the homologous, full-length HBV QS after perinatal infection and their clinical implications, five pairs of mother-daughter patien...

journal_title：Journal of clinical microbiology

authors： Liu F,Yu DM,Huang SY,Yu JL,Zhang DH,Gong QM,Zhang XX

更新日期：2014-05-01 00:00:00

abstract：:A directory of test patterns and their interpretations has been prepared for identification of Enterobacteriaceae by using the 11-test API 10 S kit. The diagnostic accuracy of the directory and kit were evaluated by using records of test results for 37,476 isolates studied with the 21-test API 20 Enteric kit. Analysis...

journal_title：Journal of clinical microbiology

authors： Robertson EA,MacLowry JD

更新日期：1975-06-01 00:00:00

abstract：:Streptococcus pneumoniae is an important cause of community-acquired pneumonia. However, in this setting the diagnostic sensitivity of blood cultures is below 30%. Since during such infections changes in the amounts of S. pneumoniae may also occur in the upper respiratory tract, quantification of these bacteria in nas...

journal_title：Journal of clinical microbiology

authors： Greiner O,Day PJ,Bosshard PP,Imeri F,Altwegg M,Nadal D

更新日期：2001-09-01 00:00:00

abstract：:We compared the BD Phoenix automated microbiology system to the Bruker Biotyper (version 2.0) matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) system for identification of gram-negative bacilli, using biochemical testing and/or genetic sequencing to resolve discordant resul...

journal_title：Journal of clinical microbiology

authors： Saffert RT,Cunningham SA,Ihde SM,Jobe KE,Mandrekar J,Patel R

更新日期：2011-03-01 00:00:00

abstract：:We report the first two cases of invasive human mycoses caused by the phaeoid ascomycete, Chaetomium perlucidum, and review the English literature regarding invasive Chaetomium infections. Fatal disseminated disease involving the brain, heart, lungs, and spleen is described in an acute myelogenous leukemia patient. A ...

journal_title：Journal of clinical microbiology

authors： Barron MA,Sutton DA,Veve R,Guarro J,Rinaldi M,Thompson E,Cagnoni PJ,Moultney K,Madinger NE

更新日期：2003-11-01 00:00:00

abstract：:A comparison of three different commercial media was made to assess their recovery of anaerobic organisms from the blood stream. The three media used were the 50-ml brain heart infusion broth with added CO2 (Pfizer), the 50-ml Thiol broth with added CO2 (Difco), and the 50-ml prereduced, supplemented peptone broth in ...

journal_title：Journal of clinical microbiology

authors： Mangels JI,Lindberg LH,Vosti KL

更新日期：1977-05-01 00:00:00

abstract：:To identify Trichinella antigens suitable for high-specificity and high-sensitivity serodiagnosis of human trichinellosis, we evaluated assays using four antigens: (i) crude first-stage larval extract (CLE), (ii) O-deglycosylated CLE, (iii) tyvelose-bearing antigens (Trichinella spiralis larva group 1 [TSL-1] antigens...

journal_title：Journal of clinical microbiology

authors： Escalante M,Romarís F,Rodríguez M,Rodríguez E,Leiro J,Gárate MT,Ubeira FM

更新日期：2004-09-01 00:00:00

abstract：:We evaluated nasopharyngeal carriage of Streptococcus pneumoniae (pneumococci) in nine Alaskan communities and used an algorithm combining microbiologic, serologic, and sequential multiplex PCR (MP-PCR) techniques to serotype the isolates. After microbiological identification as pneumococci, isolates (n = 1,135) were ...

journal_title：Journal of clinical microbiology

authors： Miernyk K,Debyle C,Harker-Jones M,Hummel KB,Hennessy T,Wenger J,Rudolph K

更新日期：2011-09-01 00:00:00

abstract：:The ERA strain of rabies virus was adapted to growth on monolayers of a porcine kidney cell line (PK-2A). A reproducible plaque assay system was subsequently developed, which appears to be satisfactory for conducting plaque neutralization tests. ...

journal_title：Journal of clinical microbiology

authors： Strating A,Mitzel JR,Slater EA

更新日期：1976-08-01 00:00:00

abstract：:Resistant cells of Pseudomonas aeruginosa ATCC 9027 which were grown in the presence of 1 mg of benzalkonium chloride (BC) per ml caused only a mild conjunctivitis when they were dropped onto the scratched corneas of rabbits. In contrast, cells of the BC-sensitive parent strain induced a severe keratoconjunctivitis. I...

journal_title：Journal of clinical microbiology

authors： Adair FW,Liauw HL,Geftic SG,Gelzer J

更新日期：1975-02-01 00:00:00

abstract：:Since the discovery of the Norwalk virus (NV) by immune electron microscopy (IEM) in 1972, serologic studies with this virus have relied on particle-positive fecal material from infected volunteers as the source of antigen because it has not been possible to propagate this virus in cell culture. However, the recent cl...

journal_title：Journal of clinical microbiology

authors： Green KY,Lew JF,Jiang X,Kapikian AZ,Estes MK

更新日期：1993-08-01 00:00:00

abstract：:Influenza viruses related to the markedly antigenically divergent strains B/Yamagata/16/88 and B/Victoria/2/87 are circulating in human populations. Adults develop cross-reacting antibodies against recent and earlier influenza B virus strains after vaccination with B/Yamagata/16/88, probably because of previous influe...

journal_title：Journal of clinical microbiology

authors： Levandowski RA,Gross PA,Weksler M,Staton E,Williams MS,Bonelli J

更新日期：1991-07-01 00:00:00

abstract：:Antigens of Rhodococcus equi were analyzed by immunoblotting with naturally infected foal sera. Immunoblots of whole-cell antigen preparations of clinical isolates of R. equi revealed that major protein bands with molecular masses of 15 to 17 kDa were present in all clinical isolates tested and all isolates virulent f...

journal_title：Journal of clinical microbiology

authors： Takai S,Koike K,Ohbushi S,Izumi C,Tsubaki S

更新日期：1991-03-01 00:00:00

abstract：:We report a case where a mite egg found in urine caused diagnostic confusion. The possibility of gut or bladder mite infection should be entertained only after repeated identification of mites in urine or stool samples from a symptomatic patient with no other cause for the symptoms and where the possibilities of conta...

journal_title：Journal of clinical microbiology

authors： Dini LA,Frean JA

更新日期：2005-12-01 00:00:00

abstract：:A semisolid medium (designated Serratia differentiation medium) containing L-arabinose, ornithine, and selective inhibitor was used to differentiate three clinically encountered Serratia species. The inhibitor, Irgasan DP-300, was incorporated to eliminate false-positive reactions from most remaining Enterobacteriacea...

journal_title：Journal of clinical microbiology

authors： Gibson S,Friedman H

更新日期：1978-03-01 00:00:00

abstract：:Cytomegalovirus (CMV) DNA loads in paired leukocyte and plasma samples from 199 patient visits by 66 patients with CMV retinitis were determined. Leukocyte CMV load determinations had a greater range of values (mean, 24,587 copies/10(6) leukocytes; maximum, 539, 000) than did plasma CMV load determinations (mean, 10,3...

journal_title：Journal of clinical microbiology

authors： Jabs DA,Forman M,Enger C,Jackson JB

更新日期：1999-05-01 00:00:00

abstract：:Rotavirus is a leading cause of dehydrating diarrhea and death among infants and children globally, particularly in communities of the developing world. While numerous studies have described the complex relationships among infectious diarrhea, growth faltering, and poverty, the impact of nutritional status on suscepti...

journal_title：Journal of clinical microbiology

authors： Verkerke H,Sobuz S,Ma JZ,Petri SE,Reichman D,Qadri F,Rahman M,Haque R,Petri WA Jr

更新日期：2016-10-01 00:00:00

abstract：:The cellular fatty acid compositions and ubiquinone contents of 182 Legionella strains representing 23 species were determined by capillary gas-liquid chromatography and reverse-phase high-performance liquid chromatography, respectively. Except for the type strain of Legionella erythra (ATCC 35303T), all Legionella sp...

journal_title：Journal of clinical microbiology

authors： Lambert MA,Moss CW

更新日期：1989-03-01 00:00:00

abstract：:Indirect and double-antibody sandwich enzyme-linked immunosorbent assay techniques were compared for serotyping 42 herpes simplex virus isolates. Both procedures gave similar results, which were in complete agreement with those obtained by inhibition of the indirect hemagglutination test. The indirect technique proved...

journal_title：Journal of clinical microbiology

authors： Gerna G,Battaglia M,Revello MG,Gerna MT

更新日期：1983-05-01 00:00:00

abstract：:Early identification of tuberculosis in the clinical setting is of great importance in order for specific therapy to be swiftly initiated. MB REDOX (Heipha Diagnostika), a growth-based medium without radioactive materials, was evaluated and was compared to the BACTEC system for detection of mycobacteria, including the...

journal_title：Journal of clinical microbiology

authors： Liu YC,Huang TS,Huang WK

更新日期：1999-12-01 00:00:00