Reduced virulence of Pseudomonas aeruginosa grown in the presence of benzalkonium chloride.

abstract：:Tularemia is the zoonotic disease caused by the gram-negative coccobacillus Francisella tularensis. Its wide distribution in the environment poses a challenge for understanding the transmission, ecology, and epidemiology of the disease. F. tularensis is also considered a potential biological weapon due to its extreme ...

journal_title：Journal of clinical microbiology

authors： Versage JL,Severin DD,Chu MC,Petersen JM

更新日期：2003-12-01 00:00:00

abstract：:A selective medium (CFAT) was developed for the detection and enumeration of Actinomyces viscosus and Actinomyces naeslundii in dental plaque. Neutral acriflavin and potassium tellurite were used in combination with the known selective agents cadmium and fluoride to eliminate most of the competing plaque flora. Compos...

journal_title：Journal of clinical microbiology

authors： Zylber LJ,Jordan HV

更新日期：1982-02-01 00:00:00

abstract：:The detection of porphyrins produced from omega-aminolevulinic acid was evaluated for use in speciation of the Haemophilus. Two hundred and forty-eight clinical isolates of nonhemolytic Haemophilus were tested concurrently for porphyrin production and for X and V requirements. The porphyrin production test was more ra...

journal_title：Journal of clinical microbiology

authors： Lund ME,Blazevic DJ

更新日期：1977-02-01 00:00:00

abstract：:The diagnosis of congenital syphilis in apparently healthy infants continues to be problematic. Immunoglobulin M antibodies specific for a subset of Treponema pallidum antigens have been detected by Western blotting (immunoblotting). In the present study we investigated the sensitivity and specificity of this method. ...

journal_title：Journal of clinical microbiology

authors： Meyer MP,Eddy T,Baughn RE

更新日期：1994-03-01 00:00:00

abstract：:The coelomycete Colletotrichum gloeosporioides was isolated in pure culture from subcutaneous nodules of the left forearm and elbow of a farmer after traumatic injury. To our knowledge, we report the first case involving this fungus as an etiological agent of subcutaneous infection. The in vitro inhibitory activities ...

journal_title：Journal of clinical microbiology

authors： Guarro J,Svidzinski TE,Zaror L,Forjaz MH,Gené J,Fischman O

更新日期：1998-10-01 00:00:00

abstract：:The CO2, O2, and pH levels of commercially available blood culture bottles with tryptic soy broth medium from five different manufacturers were compared. Ranges of 1.3 to 6.9% for CO2, 1.1 to 6.0% for O2, and pH 6.94 to 7.26 were found. Different venting procedures revealed that blood culture bottles from which the ru...

journal_title：Journal of clinical microbiology

authors： Welch WD,Porschen RK,Zarifi SZ

更新日期：1984-11-01 00:00:00

abstract：:In a prospective study, 139 serial blood samples from 15 transplant recipients were assessed for the presence of cytomegalovirus (CMV) by virus isolation (CMV viremia) and by direct staining of CMV antigens (CMV Ag) in blood leukocytes (CMV antigenemia). CMV was isolated from 23 samples, whereas CMV Ag was detected in...

journal_title：Journal of clinical microbiology

authors： van der Bij W,Schirm J,Torensma R,van Son WJ,Tegzess AM,The TH

更新日期：1988-12-01 00:00:00

abstract：:Reagent-impregnated filter paper replica methods are described which can be used to detect all penicillinase (beta-lactamase)-producing Neisseria gonorrhoeae (PPNG) colonies in an entire population of Neisseria gonorrhoeae growing on primary isolation plates. ...

journal_title：Journal of clinical microbiology

authors： Bae BH,Ledesma G,Korzis J

更新日期：1983-03-01 00:00:00

abstract：:Twenty-three young adult rhesus monkeys from China were evaluated for the presence of Helicobacter pylori. Gastric body and antral biopsy samples were tested for H. pylori by PCR analysis, culture, rapid urease testing, and histologic evaluation. Serologic testing to detect H. pylori immunoglobulin G (IgG) antibodies ...

journal_title：Journal of clinical microbiology

authors： Handt LK,Fox JG,Yan LL,Shen Z,Pouch WJ,Ngai D,Motzel SL,Nolan TE,Klein HJ

更新日期：1997-01-01 00:00:00

abstract：:To evaluate the risk of a nosocomial spread of Pneumocystis carinii f. sp. hominis (P. carinii hominis), air filter samples from rooms of P. carinii pneumonia (PCP) patients, adjacent corridors, and other hospital environments have been investigated for the presence of P. carinii hominis. Amplified DNA from air filter...

journal_title：Journal of clinical microbiology

authors： Olsson M,Lidman C,Latouche S,Björkman A,Roux P,Linder E,Wahlgren M

更新日期：1998-06-01 00:00:00

abstract：:Colony phenotype and genetic similarity were assessed within and between groups of commensal and pathogenic strains of Candida albicans collected from the oral cavities of individuals in a single geographical locale. Thirty-eight percent of pathogenic isolates contained predominant or minor variant colony morphologies...

journal_title：Journal of clinical microbiology

authors： Hellstein J,Vawter-Hugart H,Fotos P,Schmid J,Soll DR

更新日期：1993-12-01 00:00:00

abstract：:A PCR for protein D (hpd#3) was used to differentiate nontypeable Haemophilus influenzae (NTHI) from Haemophilus haemolyticus. While 90% of nasopharyngeal specimens and 100% of lower-airway specimens from 84 Indigenous Australian children with bronchiectasis had phenotypic NTHI isolates confirmed as H. influenzae, onl...

journal_title：Journal of clinical microbiology

authors： Hare KM,Binks MJ,Grimwood K,Chang AB,Leach AJ,Smith-Vaughan H

更新日期：2012-07-01 00:00:00

abstract：:Three cloned segments of Mycobacterium tuberculosis DNA which are promising as clinical probes were identified. An MboI digest of DNA from a clinical isolate of M. tuberculosis was cloned into bacteriophage M13. To identify recombinants specific for the M. tuberculosis complex, plaque lifts were hybridized with M. bov...

journal_title：Journal of clinical microbiology

authors： Eisenach KD,Crawford JT,Bates JH

更新日期：1988-11-01 00:00:00

abstract：:A batch of fetal calf serum was encountered which suppressed the number of Chlamydia trachomatis inclusions seen when it was used in conjunction with cycloheximide-treated McCoy cells. The batch of serum did not affect the number of inclusions detected in irradiated or untreated McCoy cells. ...

journal_title：Journal of clinical microbiology

authors： Evans RT

更新日期：1980-04-01 00:00:00

abstract：:Respiratory diphtheria, characterized by a firmly adherent pseudomembrane, is caused by toxin-producing strains of Corynebacterium diphtheriae, with similar illness produced occasionally by toxigenic Corynebacterium ulcerans or, rarely, Corynebacterium pseudotuberculosis While diphtheria laboratory confirmation requir...

journal_title：Journal of clinical microbiology

authors： Williams MM,Waller JL,Aneke JS,Weigand MR,Diaz MH,Bowden KE,Simon AK,Peng Y,Xiaoli L,Cassiday PK,Winchell J,Tondella ML

更新日期：2020-09-22 00:00:00

abstract：:While the majority of avian influenza virus (AIV) subtypes are classified as low-pathogenicity avian influenza viruses (LPAIV), the H5 and H7 subtypes have the ability to mutate to highly pathogenic avian influenza viruses (HPAIV) in poultry and therefore are the etiological agents of notifiable AIV (NAIV). It is of g...

journal_title：Journal of clinical microbiology

authors： Leijon M,Ullman K,Thyselius S,Zohari S,Pedersen JC,Hanna A,Mahmood S,Banks J,Slomka MJ,Belák S

更新日期：2011-11-01 00:00:00

abstract：:The API Staph-Ident system was evaluated as a means for identifying the species of bovine strains of staphylococci routinely isolated from quarter-milk samples. The species identity of 314 of 581 (54%) isolates of staphylococci was correctly determined by this method. The API Staph-Ident system was more accurate in co...

journal_title：Journal of clinical microbiology

authors： Langlois BE,Harmon RJ,Akers K

更新日期：1983-11-01 00:00:00

abstract：:A ligation-mediated PCR (LMPCR) method for the amplification of sequences flanking the IS6110 of the Mycobacterium tuberculosis complex has been developed. The method uses one primer specific for IS6110 and a second specific for a linker ligated to SalI-restricted genomic DNA. LMPCR is a rapid screening method, valuab...

journal_title：Journal of clinical microbiology

authors： Prod'hom G,Guilhot C,Gutierrez MC,Varnerot A,Gicquel B,Vincent V

更新日期：1997-12-01 00:00:00

abstract：:Automated DNA sequencing was used to characterize mutations associated with rifampin resistance in a 69-bp region of the gene, rpoB, encoding the beta subunit of RNA polymerase in Mycobacterium tuberculosis. The data confirmed that greater than 90% of rifampin-resistant strains have sequence alterations in this region...

journal_title：Journal of clinical microbiology

authors： Kapur V,Li LL,Iordanescu S,Hamrick MR,Wanger A,Kreiswirth BN,Musser JM

更新日期：1994-04-01 00:00:00

abstract：:Oligonucleotide chips targeting the bacterial internal transcribed spacer region (ITS) of the 16S-23S rRNA gene, which contains genus- and species-specific regions, were developed and evaluated. Forty-three sequences were designed consisting of 1 universal, 3 Gram stain-specific, 9 genus-specific, and 30 species-speci...

journal_title：Journal of clinical microbiology

authors： Kim CM,Song ES,Jang HJ,Kim HJ,Lee S,Shin JH,Kim SJ,Jeong SH,Jeong J,Koh K,Choi GE,Lee EY,Chang CL

更新日期：2010-05-01 00:00:00

abstract：:This study compared four methods for serotyping isolates of Haemophilus influenzae. Slide agglutination with commercial antisera (Difco Laboratories and Wellcome Diagnostics), coagglutination (Phadebact Haemophilus Test [Pharmacia Fine Chemicals]), latex agglutination with affinity-purified anticapsular antibody, and ...

journal_title：Journal of clinical microbiology

authors： Himmelreich CA,Barenkamp SJ,Storch GA

更新日期：1985-02-01 00:00:00

abstract：:Rhodococcus equi infection is increasing in regions with high HIV prevalence worldwide. The microbiological features and clinical mimicry of tuberculosis infection pose diagnostic challenges in high-tuberculosis-incidence settings. We present two HIV-associated cases of R. equi infection from Vietnam and discuss the u...

journal_title：Journal of clinical microbiology

authors： Le T,Cash-Goldwasser S,Tho PV,Lan NP,Campbell JI,van Doorn HR,Lam NT,Trung NV,Trinh DT,Van Kinh N,Wertheim HF

更新日期：2015-04-01 00:00:00

abstract：:Clostridium difficile is the leading cause of antibiotic-associated diarrhea and pseudomembranous colitis, which have significant morbidity and mortality. Accurate and timely diagnosis is critical. Repeat enzyme immunoassay testing for C. difficile toxin has been recommended because of <100% sensitivity. All C. diffic...

journal_title：Journal of clinical microbiology

authors： Cardona DM,Rand KH

更新日期：2008-11-01 00:00:00

abstract：:Anthopsis deltoidea was found to be the cause of an olecranon bursitis in a 79-year-old golfer. Serial histological sections of the olecranon bursa showed faintly stained, brown-walled, septate, hyphal elements in the centers of the necrotic debris. The combination of bursectomy and flucytosine treatment cured the inf...

journal_title：Journal of clinical microbiology

authors： Kwon-Chung KJ,Droller DD

更新日期：1984-08-01 00:00:00

abstract：:Several G8P[6] and G8P[8] rotavirus strains were isolated from hospitalized patients in the Democratic Republic of Congo in 2003. To investigate their overall genomic relatedness and to determine to which genogroup they belonged, the complete genomes of strains DRC88 (G8P[8]) and DRC86 (G8P[6]) were determined. Genomi...

journal_title：Journal of clinical microbiology

authors： Matthijnssens J,Rahman M,Yang X,Delbeke T,Arijs I,Kabue JP,Muyembe JJ,Van Ranst M

更新日期：2006-05-01 00:00:00

abstract：:To evaluate the accuracy and utility of the Eiken Systek No. 1 (Eiken system; Eiken Chemical Co., Ltd., Tokyo, Japan), we conducted a clinical comparison, with 345 Enterobacteriaceae isolates, of the Eiken System with API 20E (Analytab Products, Inc., Plainview, N.Y.) and conventional methods. The Eiken system is a 21...

journal_title：Journal of clinical microbiology

authors： Castillo CB,Bruckner DA

更新日期：1984-10-01 00:00:00

abstract：:The nasopharyngeal Haemophilus influenzae flora of healthy children in a day care center was analyzed by repeated sampling during 4 winter months. The average carrier rate was 39%, but 74% of the children became colonized at some time during the study. The H. influenzae isolates were identified by capsular type, bioty...

journal_title：Journal of clinical microbiology

authors： Trottier S,Stenberg K,Svanborg-Edén C

更新日期：1989-10-01 00:00:00

abstract：:The frequency with which clue cells could be detected in Gram-stained vaginal smears and/or cervical Papanicolaou (Pap) smears was compared with the frequency of Corynebacterium vaginale (Haemophilus vaginalis) isolation in a group of 236 female patients, of whom 221 had vaginitis. Vaginal clue cells were found most o...

journal_title：Journal of clinical microbiology

authors： Smith RF,Rodgers HA,Hines PA,Ray RM

更新日期：1977-03-01 00:00:00

abstract：:About 2.5 years after the first isolation of the VanA phenotype of vancomycin-resistant Enterococcus faecium (VREM) in Poland, the first VREM strains with the VanB phenotype have emerged independently in two different Warsaw hospitals. In one of these the VREM strain was selected during the long-term antimicrobial tre...

journal_title：Journal of clinical microbiology

authors： Kawalec M,Gniadkowski M,Zielińska U,Kłos W,Hryniewicz W

更新日期：2001-02-01 00:00:00

abstract：:A slightly modified Moeller medium for amino acid decarboxylase and dihydrolase testing by the replicator method correlated 96 to 99% with the reference Moeller broth method. False positive results were not encountered in this study. ...

journal_title：Journal of clinical microbiology

authors： Jones RN,Fuchs PC,Sniderman S

更新日期：1976-01-01 00:00:00