Abstract:
:A urease-based enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of reagin antibodies in serum. Visuwell Reagin (ADI Diagnostics Inc., Rexdale, Ontario, Canada) is a non-treponemal screening test for the serodiagnosis of syphilis which has the benefits of large batch testing, automatability, and objective interpretation of results. Unheated, undiluted sera are incubated in 96-well microtiter plates coated with a modified cardiolipin-lecithin-cholesterol antigen. Antibody bound to the plate is detected by an anti-human immunoglobulin G-urease conjugate. The procedure consists of three steps, with a total test time of 60 min. Visuwell Reagin ELISA was compared with the Venereal Disease Research Laboratory (VDRL) test and the reagin screening test (RST) with the following results. For ELISA versus the VDRL test, the sensitivities for untreated syphilis (n = 37) were 97.3% for both ELISA and the VDRL test, the confirmatory positive values (n = 79) were 84.8% for ELISA and 72.2% for the VDRL test, and the specificities for normal samples (n = 1,327) were 98.8% for ELISA and 99.5% for the VDRL test. For ELISA versus RST, the sensitivities for untreated syphilis (n = 57) were 94.7% for ELISA and 87.7% for RST, the confirmatory positive values (n = 26) were 96.2% for ELISA and 92.3% for RST, and the specificities for normal samples (n = 1,891) were 99.6% for ELISA and 99.3% for RST. The overall concordance values of ELISA with VDRL test and RST were 96.7 and 97.9%, respectively. The specificity of ELISA compared with that of RST may be underestimated, since confirmatory data were not available for all apparent false-positive samples. Visuwell Reagin had increased sensitivity and similar specificity compared with flocculation tests.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
White TJ,Fuller SAdoi
10.1128/JCM.27.10.2300-2304.1989subject
Has Abstractpub_date
1989-10-01 00:00:00pages
2300-4issue
10eissn
0095-1137issn
1098-660Xjournal_volume
27pub_type
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.27.11.2414-2419.1989
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.18.4.770-774.1983
更新日期:1983-10-01 00:00:00
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pub_type: 杂志文章,多中心研究
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.20.4.775-779.1984
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.31.4.887-894.1993
更新日期:1993-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-07-01 00:00:00
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.23.5.970-972.1986
更新日期:1986-05-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.26.9.1626-1629.1988
更新日期:1988-09-01 00:00:00
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journal_title:Journal of clinical microbiology
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doi:
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abstract::Branched DNA (bDNA) assays to quantify human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV) consist of three distinct steps, including sample processing, hybridization, and detection, and utilize the System 340 platform for plate incubation and washing. Sample processing differs: HIV-1 from 1 ml of ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.42.7.3120-3127.2004
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.32.5.1364-1365.1994
更新日期:1994-05-01 00:00:00
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.36.10.2893-2899.1998
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.37.3.484-489.1999
更新日期:1999-03-01 00:00:00
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.25.5.840-844.1987
更新日期:1987-05-01 00:00:00
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.18.5.1141-1145.1983
更新日期:1983-11-01 00:00:00
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更新日期:2012-04-01 00:00:00
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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