Reproducibility of linear array for human papillomavirus genotyping.

abstract：:A solid-phase antibody capture hemadsorption (SPACH) assay was developed to detect hepatitis A virus (HAV)-specific immunoglobulin M (IgM) antibodies in sera from humans recently infected with hepatitis. The assay is performed with microtiter plates coated with anti-human IgM antibodies to capture IgM antibodies from ...

journal_title：Journal of clinical microbiology

authors： Summers PL,Dubois DR,Cohen WH,Macarthy PO,Binn LN,Sjogren MH,Snitbhan R,Innis BL,Eckels KH

更新日期：1993-05-01 00:00:00

abstract：:Baculovirus-expressed foot-and-mouth disease virus (FMDV) nonstructural protein 3AB was used as the antigen in an enzyme-linked immunosorbent assay. This assay allowed the differentiation of vaccinated from infected pigs. Serial studies were performed using sera collected from pigs in the field. Positive reactions wer...

journal_title：Journal of clinical microbiology

authors： Chung WB,Sorensen KJ,Liao PC,Yang PC,Jong MH

更新日期：2002-08-01 00:00:00

abstract：:The reliability of the novel BACTEC MGIT 960 pyrazinamide (PZA) kit (Becton Dickinson Microbiology Systems, Sparks, Md.) was assessed for testing of susceptibility of Mycobacterium tuberculosis to PZA. Results generated by the BACTEC MGIT 960 system (Becton Dickinson) were compared with those obtained with the BACTEC ...

journal_title：Journal of clinical microbiology

authors： Pfyffer GE,Palicova F,Rüsch-Gerdes S

更新日期：2002-05-01 00:00:00

abstract：:We present a duplex, real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) genes. Accuracy was assessed with 158 Gram-negative bacillary isolates, including 134 carbapenemase producers. The assay had 100% sensitivity and specificity compared with r...

journal_title：Journal of clinical microbiology

authors： Cunningham SA,Noorie T,Meunier D,Woodford N,Patel R

更新日期：2013-04-01 00:00:00

abstract：:Ten clinical yeast isolates submitted to the Centers for Disease Control from diverse geographic areas were identified as Candida ciferrii and Candida chiropterorum. The association of C. ciferrii with clinical specimens, particularly its repeated isolation from a case of onychomycosis, suggests that this species may ...

journal_title：Journal of clinical microbiology

authors： Furman RM,Ahearn DG

更新日期：1983-11-01 00:00:00

abstract：:Twelve bacterial species including Streptococcus uberis, S. parauberis, S. agalactiae, S. dysgalactiae, S. bovis, S. mitis, S. salivarius, S. saccharolyticus, Enterococcus faecium, E. faecalis, E. avium, and Aerococcus viridans were examined for their 16S ribosomal DNA fingerprint patterns. Oligonucleotide primers com...

journal_title：Journal of clinical microbiology

authors： Jayarao BM,Doré JJ Jr,Oliver SP

更新日期：1992-09-01 00:00:00

abstract：:This study compared the abilities of API 20E and BBL Crystal E/NF identification systems to correctly identify human and animal source gram-negative bacilli of known identifications, as provided by the American Type Culture Collection, Rockville, Md., and the Research Diagnostic and Investigative Laboratory, Columbia,...

journal_title：Journal of clinical microbiology

authors： Peele D,Bradfield J,Pryor W,Vore S

更新日期：1997-01-01 00:00:00

abstract：:A PCR test based on insertion sequence IS1081 was developed to detect Mycobacterium tuberculosis complex organisms in the peripheral blood. The method was applied to blood samples from immunocompetent individuals with localized pulmonary tuberculosis. Seven of 16 (43.75%) blood samples were found to be positive for th...

journal_title：Journal of clinical microbiology

authors： Ahmed N,Mohanty AK,Mukhopadhyay U,Batish VK,Grover S

更新日期：1998-10-01 00:00:00

abstract：:We evaluated a newly commercial enzyme immunoassay (EIA) (Biotest Legionella Urin Antigen EIA; Biotest AG, Dreieich, Germany) for detection of antigens of all Legionella pneumophila serogroups with a relatively wide spectrum of cross-reactivity as well as antigens of other Legionella spp. by comparing its sensitivity ...

journal_title：Journal of clinical microbiology

authors： Domínguez JA,Galí N,Pedroso P,Fargas A,Padilla E,Manterola JM,Matas L

更新日期：1998-09-01 00:00:00

abstract：:The AutoMicrobic system (AMS; Vitek Systems, Inc., Hazelwood, Mo.) was studied for its ability to produce accurate and precise MIC interpretations for Pseudomonas aeruginosa susceptibility to gentamicin, tobramycin, and amikacin. MICs were determined in parallel on 200 selected P. aeruginosa isolates by using the AMS ...

journal_title：Journal of clinical microbiology

authors： Woolfrey BF,Lally RT,Ederer MN,Quall CO

更新日期：1984-04-01 00:00:00

abstract：:The CLSI (formerly NCCLS) M38-A document for antifungal susceptibility testing of filamentous fungi does not describe guidelines for echinocandins. A multicenter study (eight centers) evaluated inter- and intralaboratory reproducibilities of two reading times (24 and 48 h or 48 and 72 h) and two end points (MICs and m...

journal_title：Journal of clinical microbiology

authors： Espinel-Ingroff A,Fothergill A,Ghannoum M,Manavathu E,Ostrosky-Zeichner L,Pfaller MA,Rinaldi MG,Schell W,Walsh TJ

更新日期：2007-07-01 00:00:00

abstract：:Antibody to equid herpesvirus 1, which mediates single radial hemolysis, is that responsible for neutralization. Hemagglutination inhibition antibody is not necessarily involved in neutralization or hemolysis. ...

journal_title：Journal of clinical microbiology

authors： Klingeborn B,Dinter Z

更新日期：1978-05-01 00:00:00

abstract：:The biochemical characteristics and the presence of human Fc(gamma) receptors of 52 throat isolates of group C beta-hemolytic streptococci were examined. Among these isolates, 38 were identified as "Streptococcus milleri" and 14 were identified as "Streptococcus equisimilis." The differentiation of group C "S. equisim...

journal_title：Journal of clinical microbiology

authors： Lebrun L,Guibert M,Wallet P,de Maneville MM,Pillot J

更新日期：1986-11-01 00:00:00

abstract：:Pneumocystis jirovecii pneumonia (PCP) is a life-threatening infection in immunocompromised patients. Quantitative real-time PCR (qPCR) is more sensitive than microscopic examination for the detection of P. jirovecii but also detects colonized patients. Hence, its positive predictive value (PPV) needs evaluation. In t...

journal_title：Journal of clinical microbiology

authors： Robert-Gangneux F,Belaz S,Revest M,Tattevin P,Jouneau S,Decaux O,Chevrier S,Le Tulzo Y,Gangneux JP

更新日期：2014-09-01 00:00:00

abstract：:We describe a Klebsiella oxytoca infection outbreak in a renal transplant unit that involved seven patients. All strains belonged to a single pulsed-field gel electrophoresis pattern and were resistant to amoxicillin-clavulanate, cefuroxime, piperacillin-tazobactam, and aztreonam but susceptible to ceftriaxone, ceftaz...

journal_title：Journal of clinical microbiology

authors： Zárate MS,Gales AC,Picão RC,Pujol GS,Lanza A,Smayevsky J

更新日期：2008-06-01 00:00:00

abstract：:Burkholderia pseudomallei is the causative agent of melioidosis. In northeast Thailand, this gram-negative bacterium is a major cause of mortality from septicemia. The definitive diagnosis of this disease is made by bacterial culture. In this study, we produced a monoclonal antibody (MAb) specific to the 30-kDa protei...

journal_title：Journal of clinical microbiology

authors： Pongsunk S,Thirawattanasuk N,Piyasangthong N,Ekpo P

更新日期：1999-11-01 00:00:00

abstract：:Resistant cells of Pseudomonas aeruginosa ATCC 9027 which were grown in the presence of 1 mg of benzalkonium chloride (BC) per ml caused only a mild conjunctivitis when they were dropped onto the scratched corneas of rabbits. In contrast, cells of the BC-sensitive parent strain induced a severe keratoconjunctivitis. I...

journal_title：Journal of clinical microbiology

authors： Adair FW,Liauw HL,Geftic SG,Gelzer J

更新日期：1975-02-01 00:00:00

abstract：:A total of 513 blood specimens, predominantly from organ transplant recipients, human immunodeficiency virus-positive patients, and bone marrow transplant recipients, were tested for cytomegalovirus (CMV) by culture and pp65 antigenemia across four test sites. Peripheral blood leukocytes were examined by using both th...

journal_title：Journal of clinical microbiology

authors： St George K,Boyd MJ,Lipson SM,Ferguson D,Cartmell GF,Falk LH,Rinaldo CR,Landry ML

更新日期：2000-04-01 00:00:00

abstract：:Quantitative real-time PCR for the detection of respiratory syncytial virus (RSV) RNA is increasingly used to study the causal role of RSV in lower airway disease. The objective of our study was to evaluate variations in RSV RNA loads at different steps in the RNA quantification process: (i) variation in RSV RNA load ...

journal_title：Journal of clinical microbiology

authors： van de Pol AC,Wolfs TF,van Loon AM,Tacke CE,Viveen MC,Jansen NJ,Kimpen JL,Rossen JW,Coenjaerts FE

更新日期：2010-10-01 00:00:00

abstract：:A duplex PCR to detect Bordetella pertussis and Bordetella parapertussis was developed with the insertion sequences IS481 (B. pertussis) and IS1001 (B. parapertussis) and evaluated with specimens from 520 consecutive patients presenting with possible pertussis. No culture-positive-PCR-negative results occurred, giving...

journal_title：Journal of clinical microbiology

authors： Farrell DJ,Daggard G,Mukkur TK

更新日期：1999-03-01 00:00:00

abstract：:Direct inoculation onto Granada medium (GM) in plates and tubes was compared to inoculation into a selective Todd-Hewitt broth (with 8 microg of gentamicin per ml and 15 microg of nalidixic acid per ml) for detection of group B streptococci (GBS) in pregnant women with 800 vaginal and 450 vaginoanorectal samples. Comp...

journal_title：Journal of clinical microbiology

authors： Rosa-Fraile M,Rodriguez-Granger J,Cueto-Lopez M,Sampedro A,Gaye EB,Haro JM,Andreu A

更新日期：1999-08-01 00:00:00

abstract：:Mycobacterium microti is a member of the Mycobacterium tuberculosis complex of bacteria. This species was originally identified as a pathogen of small rodents and shrews and was associated with limited diversity and a much reduced spoligotype pattern. More recently, specific deletions of chromosomal DNA have been show...

journal_title：Journal of clinical microbiology

authors： Smith NH,Crawshaw T,Parry J,Birtles RJ

更新日期：2009-08-01 00:00:00

abstract：:Two rapid (1-h) assays for the detection of Staphylococcus aureus staphylocoagulase were developed by using the fluorogenic thrombin substrates N-t-boc-Val-Pro-Arg-7-amido-4-methylcoumarin (VPA) and N-t-boc-beta-benzyl-Asp-Pro-Arg-7-amido-4-methylocoumarin (BB). The assays were compared to the tube coagulase test and ...

journal_title：Journal of clinical microbiology

authors： Holliday MG,Ford M,Perry JD,Gould FK

更新日期：1999-04-01 00:00:00

abstract：:Traveler's diarrhea (TD) is the most common infectious illness acquired by visitors to developing nations. The purpose of this study was to utilize molecular diagnostic techniques to determine the prevalence of norovirus (NoV) in TD occurring among visitors from the United States to Guatemala and Mexico. Stool samples...

journal_title：Journal of clinical microbiology

authors： Chapin AR,Carpenter CM,Dudley WC,Gibson LC,Pratdesaba R,Torres O,Sanchez D,Belkind-Gerson J,Nyquist I,Kärnell A,Gustafsson B,Halpern JL,Bourgeois AL,Schwab KJ

更新日期：2005-03-01 00:00:00

abstract：:The applicability of whole-cell hybridization for the identification of pathogenic bacteria in blood from septic patients was examined. Oligonucleotide probes, fluorescently labeled with fluorescein isothiocyanate, directed against the variable regions of the 16S rRNAs of the following bacterial species and/or genera ...

journal_title：Journal of clinical microbiology

authors： Jansen GJ,Mooibroek M,Idema J,Harmsen HJ,Welling GW,Degener JE

更新日期：2000-02-01 00:00:00

abstract：:Antibiogram typing of methicillin-resistant Staphylococcus aureus with selected antibiotics was evaluated as a primary epidemiological typing tool and compared with ribotyping. Antibiograms were derived with the Kirby-Bauer disk diffusion method by using erythromycin, clindamycin, cotrimoxazole, gentamicin, and ciprof...

journal_title：Journal of clinical microbiology

authors： Blanc DS,Lugeon C,Wenger A,Siegrist HH,Francioli P

更新日期：1994-10-01 00:00:00

abstract：:Vaginal swab specimens may be preferable to cervical swab or urine specimens for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae because of the ease of specimen collection and transport. The purpose of this study was to evaluate whether vaginal swab specimens are equivalent to cervical swab specimens ...

journal_title：Journal of clinical microbiology

authors： Cosentino LA,Landers DV,Hillier SL

更新日期：2003-08-01 00:00:00

abstract：:We compared the Mycoplasma Duo kit (Sanofi Diagnostics Pasteur) with PCR for detection of Ureaplasma spp. in endotracheal aspirates from 60 premature neonates. The overall agreement between the two tests was 96%. The Mycoplasma Duo assay is a useful alternative to culture and PCR for detection of neonatal Ureaplasma i...

journal_title：Journal of clinical microbiology

authors： Cheah FC,Anderson TP,Darlow BA,Murdoch DR

更新日期：2005-01-01 00:00:00

abstract：:The COVID-19 pandemic has necessitated a multifaceted rapid response by the scientific community, bringing researchers, health officials, and industry together to address the ongoing public health emergency. To meet this challenge, participants need an informed approach for working safely with the etiological agent, t...

journal_title：Journal of clinical microbiology

authors： Welch SR,Davies KA,Buczkowski H,Hettiarachchi N,Green N,Arnold U,Jones M,Hannah MJ,Evans R,Burton C,Burton JE,Guiver M,Cane PA,Woodford N,Bruce CB,Roberts ADG,Killip MJ

更新日期：2020-10-21 00:00:00

abstract：:Immunoblots were used to study the immunoglobulin G response to Borrelia burgdorferi in experimentally and naturally exposed dogs. Adsorption studies confirmed that the antibodies were specific for B. burgdorferi. Experimentally exposed dogs were asymptomatic. Naturally exposed dogs included both asymptomatic animals ...

journal_title：Journal of clinical microbiology

authors： Greene RT,Walker RL,Nicholson WL,Heidner HW,Levine JF,Burgess EC,Wyand M,Breitschwerdt EB,Berkhoff HA

更新日期：1988-04-01 00:00:00