Abstract:
:Traveler's diarrhea (TD) is the most common infectious illness acquired by visitors to developing nations. The purpose of this study was to utilize molecular diagnostic techniques to determine the prevalence of norovirus (NoV) in TD occurring among visitors from the United States to Guatemala and Mexico. Stool samples (n = 54) were collected from 34 TD cases and analyzed for NoV by reverse transcription-PCR and oligoprobe confirmation. The overall prevalence of NoV was 65%. Interestingly, all NoV-positive stool samples were identified as genogroup I NoVs, and time spent at travel destinations was found to be an important factor in determining the frequency of infection (P = 0.003). Eleven NoV-positive stool samples also tested positive for enterotoxigenic Escherichia coli, indicating that dual infections with this leading bacterial cause of TD were very common. Results of this study suggest that NoV infection is a frequent occurrence among travelers to Mexico and Guatemala who experience episodes of TD. In addition, the simple molecular detection method utilized here will serve to facilitate more in-depth epidemiological studies of this emergent viral pathogen in travelers and other at-risk populations.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Chapin AR,Carpenter CM,Dudley WC,Gibson LC,Pratdesaba R,Torres O,Sanchez D,Belkind-Gerson J,Nyquist I,Kärnell A,Gustafsson B,Halpern JL,Bourgeois AL,Schwab KJdoi
10.1128/JCM.43.3.1112-1117.2005subject
Has Abstractpub_date
2005-03-01 00:00:00pages
1112-7issue
3eissn
0095-1137issn
1098-660Xpii
43/3/1112journal_volume
43pub_type
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