Cellulosimicrobium funkei: first report of infection in a nonimmunocompromised patient and useful phenotypic tests for differentiation from Cellulosimicrobium cellulans and Cellulosimicrobium terreum.

abstract：:An unusual Enterocytozoon bieneusi genotype was found in seven guinea pigs and a 2-year-old child in the same household. The genetic uniqueness of the parasite, its wide occurrence in other guinea pigs in the community, and its absence in other children in the community suggest the possibility of zoonotic transmission...

journal_title：Journal of clinical microbiology

authors： Cama VA,Pearson J,Cabrera L,Pacheco L,Gilman R,Meyer S,Ortega Y,Xiao L

更新日期：2007-08-01 00:00:00

abstract：:A three-step method for the purification of Enterocytozoon bieneusi spores from stool specimens was developed. The primary process of purification of the spores from bacterial contaminants involved Percoll gradient centrifugation followed by additional separation using cesium chloride density gradient centrifugation. ...

journal_title：Journal of clinical microbiology

authors： Kucerova Z,Moura H,Leitch GJ,Sriram R,Bern C,Kawai V,Vargas D,Gilman RH,Ticona E,Vivar A,Visvesvara GS

更新日期：2004-07-01 00:00:00

abstract：:A Swedish bovine and a Dutch human phage set for coagulase-negative staphylococci were used to phage type coagulase-negative staphylococci isolated from bovine milk from Minnesota dairy herds. A comparison was also made of the deoxyribonuclease activity of coagulase-negative staphylococci isolated from bovine milk in ...

journal_title：Journal of clinical microbiology

authors： Holmberg O,Lyon RH,Price D,Farnsworth RJ

更新日期：1979-09-01 00:00:00

abstract：:Dried blood spot (DBS) specimens were assessed as an alternative to plasma for human immunodeficiency virus type 1 (HIV-1) serotyping by V3 loop peptide enzyme immunoassay. Nested PCR capable of distinguishing HIV-1 subtypes B and E was used as the reference standard. Ninety-two percent of DBS samples were typeable as...

journal_title：Journal of clinical microbiology

authors： Chanbancherd P,Brown AE,Trichavaroj R,Tienamporn P,Puthakird P,Limpairojn N,VanCott TC,de Souza MS

更新日期：1999-03-01 00:00:00

abstract：:The development of diagnostic susceptibility tests for CEM-101, a new fluoroketolide, was addressed by structured studies to determine the optimal disk diffusion test concentration and effects of various testing conditions or supplements and to establish the quality control (QC) ranges for reference broth microdilutio...

journal_title：Journal of clinical microbiology

authors： Jones RN,Ross JE,Rhomberg PR,Quality Control Working Group.

更新日期：2010-04-01 00:00:00

abstract：:The incidence of enterotoxigenic Escherichia coli (ETEC) was investigated in 95 E. coli strains isolated from 48 infants with diarrhea in Santiago, Chile. By using standard biological assays and DNA-DNA hybridization procedures, ETEC was found in 31.2% of the cases: 14 strains produced heat-stable enterotoxin (ST) onl...

journal_title：Journal of clinical microbiology

authors： Agüero ME,Reyes L,Prado V,Orskov I,Orskov F,Cabello FC

更新日期：1985-10-01 00:00:00

abstract：:A search has been made for corynebacterial phospholipase D, "ovis toxin," a sphingomyelinase (phosphatidylcholine phosphohydrolase, EC 3.1.4.4), among a wide variety of corynebacteria. Phospholipase D activity has been found in strains exhibiting the biochemical properties characteristic of Corynebacterium pseudotuber...

journal_title：Journal of clinical microbiology

authors： Barksdale L,Linder R,Sulea IT,Pollice M

更新日期：1981-02-01 00:00:00

abstract：:Carbapenem-resistant Acinetobacter baumannii isolates were obtained from 24 patients between March and May 2004 at the Centre Hospitalier de Polynésie Française, Tahiti, French Polynesia. The isolates were multidrug resistant, produced the carbapenemase OXA-23, and belonged to a single clone presenting several subtype...

journal_title：Journal of clinical microbiology

authors： Naas T,Levy M,Hirschauer C,Marchandin H,Nordmann P

更新日期：2005-09-01 00:00:00

abstract：:We report five new IS711 chromosomal locations that are specific for marine mammal Brucella groups of strains and useful for their identification and classification. Our data support their current classification into two species, Brucella ceti and B. pinnipedialis, with subgroups in each, but also the possibility of a...

journal_title：Journal of clinical microbiology

authors： Zygmunt MS,Maquart M,Bernardet N,Doublet B,Cloeckaert A

更新日期：2010-10-01 00:00:00

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) of sequence type 398 (ST398) has frequently been detected in pigs and pig handlers. However, in Malaysia, sampling 360 pigs and 90 pig handlers from 30 farms identified novel ST9-spa type t4358-staphylococcal cassette chromosome mec type V MRSA strains that were found...

journal_title：Journal of clinical microbiology

authors： Neela V,Mohd Zafrul A,Mariana NS,van Belkum A,Liew YK,Rad EG

更新日期：2009-12-01 00:00:00

abstract：:Hepatitis E virus (HEV) is a major cause of acute viral hepatitis in people in many developing countries and is also endemic in many industrialized countries. Mammalian HEV (mHEV) isolates can be divided into at least four recognized major genotypes. Several nucleic acid amplification techniques have been developed fo...

journal_title：Journal of clinical microbiology

authors： Gerber PF,Xiao CT,Cao D,Meng XJ,Opriessnig T

更新日期：2014-04-01 00:00:00

abstract：:Nineteen Rhizomucor miehei and Rhizomucor pusillus isolates were assayed for their ability to utilize 87 various substrates as a single carbon source. Besides a difference in sucrose utilization, distinctive differences were found in the utilization of glycine, phenylalanine, and beta-alanine. Five isoenzyme systems a...

journal_title：Journal of clinical microbiology

authors： Vastag M,Papp T,Kasza Z,Vágvölgyi C

更新日期：1998-07-01 00:00:00

abstract：:Methicillin-resistant coagulase-negative staphylococci were isolated from the nares and skin of 1- to 8-week-old healthy chickens in three flocks from a farm. Isolation of methicillin-resistant coagulase-negative staphylococci was positive for 72 (25.7%) of the 280 chickens tested, with the frequency varying from 2.2 ...

journal_title：Journal of clinical microbiology

authors： Kawano J,Shimizu A,Saitoh Y,Yagi M,Saito T,Okamoto R

更新日期：1996-09-01 00:00:00

abstract：:The objective of the present study was the development of a diagnostic reverse transcription (RT)-PCR for the specific detection of enterovirus (EV) RNA in clinical specimens controlled by an internal control (IC) RNA. The IC RNA contains the same primer binding sites as EV RNA but has a different probe region. The IC...

journal_title：Journal of clinical microbiology

authors： Beld M,Minnaar R,Weel J,Sol C,Damen M,van der Avoort H,Wertheim-van Dillen P,van Breda A,Boom R

更新日期：2004-07-01 00:00:00

abstract：:A simple enzyme immunoassay has been developed to allow the quantitation of the human response to immunization with pneumococcal polysaccharide. The assay uses the 14-valent vaccine (Pneumovax) as a convenient antigen to adsorb to the solid-phase microdilution plate wells and commercially available isotype-specific an...

journal_title：Journal of clinical microbiology

authors： Shyamala GN,Roberton DM,Hosking CS

更新日期：1988-08-01 00:00:00

abstract：:The recently described genus Pandoraea contains five named species (Pandoraea apista, Pandoraea pulmonicola, Pandoraea pnomenusa, Pandoraea sputorum, and Pandoraea norimbergensis) and four unnamed genomospecies. Pandoraea spp. have mainly been recovered from the respiratory tracts of cystic fibrosis (CF) patients. Acc...

journal_title：Journal of clinical microbiology

authors： Coenye T,Liu L,Vandamme P,LiPuma JJ

更新日期：2001-12-01 00:00:00

abstract：:Campylobacter jejuni was recovered from four dogs (11%) and four cats (33%) living with Danish human patients infected with C. jejuni. Pulsed-field gel electrophoresis (PFGE) analysis revealed the occurrence of the same quinolone-resistant strain in a girl and her dog. C. jejuni isolates with closely related (>95% sim...

journal_title：Journal of clinical microbiology

authors： Damborg P,Olsen KE,Møller Nielsen E,Guardabassi L

更新日期：2004-03-01 00:00:00

abstract：:Two new T-cell-based reporter cell lines were established to measure human immunodeficiency virus type 1 (HIV-1) infectivity. One cell line naturally expresses CD4 and CXCR4, making it susceptible to X4-tropic viruses, and the other cell line, in which a CCR5 expression vector was introduced, is susceptible to both X4...

journal_title：Journal of clinical microbiology

authors： Chiba-Mizutani T,Miura H,Matsuda M,Matsuda Z,Yokomaku Y,Miyauchi K,Nishizawa M,Yamamoto N,Sugiura W

更新日期：2007-02-01 00:00:00

abstract：:Between September 1995 and August 1998, the incidence and diversity of the main human rotavirus genotypes (G1, G2, G3, and G4 and P[8], P[4], P[6], and P[9]) among Irish children were determined by using established and adapted reverse transcriptase PCR-based genotyping methods. From a total of 193 rotavirus-positive ...

journal_title：Journal of clinical microbiology

authors： O'Mahony J,Foley B,Morgan S,Morgan JG,Hill C

更新日期：1999-06-01 00:00:00

abstract：:A new urease-based enzyme-linked immunosorbent assay utilizing novel monoclonal antibodies was evaluated for the culture confirmation of Neisseria gonorrhoeae, with 270 isolates of N. gonorrhoeae, 56 isolates of diverse Neisseria spp., and 29 Moraxella isolates. The test was highly specific (100.00%) and sensitive (97...

journal_title：Journal of clinical microbiology

authors： Carballo M,Dillon JR,Lussier M,Milthorp P,Winston S,Brodeur B

更新日期：1992-08-01 00:00:00

abstract：:Tracking the spread of noroviruses during outbreaks of gastroenteritis is hampered by the lack of sequence diversity in those regions of the genome chosen for virus detection and characterization. Sequence analysis of regions of the genes encoding the RNA-dependent RNA polymerase and the S domain of the capsid does no...

journal_title：Journal of clinical microbiology

authors： Xerry J,Gallimore CI,Iturriza-Gómara M,Allen DJ,Gray JJ

更新日期：2008-03-01 00:00:00

abstract：:The mainstay of laboratory diagnosis for Lyme disease is two-tiered serological testing, in which a reactive first-tier enzyme-linked immunosorbent assay (ELISA) or an immunofluorescence assay is supplemented by separate IgM and IgG immunoblots. Recent data suggest that the C6 ELISA can be substituted for immunoblots ...

journal_title：Journal of clinical microbiology

authors： Wormser GP,Levin A,Soman S,Adenikinju O,Longo MV,Branda JA

更新日期：2013-12-01 00:00:00

abstract：:Recent studies have shown the public health importance of identifying individuals with acute human immunodeficiency virus infection (AHI); however, the cost of nucleic acid amplification testing (NAAT) makes individual testing of at-risk individuals prohibitively expensive in many settings. Pooled NAAT (or group testi...

journal_title：Journal of clinical microbiology

authors： Westreich DJ,Hudgens MG,Fiscus SA,Pilcher CD

更新日期：2008-05-01 00:00:00

abstract：:High-risk human papillomavirus (hrHPV) testing is now being introduced as a potential primary screening test for improved detection of cervical precancer and cancer. Current U.S. Food and Drug Administration-approved tests are batch tests that take several hours to complete. A rapid, non-batch test might permit point-...

journal_title：Journal of clinical microbiology

authors： Einstein MH,Smith KM,Davis TE,Schmeler KM,Ferris DG,Savage AH,Gray JE,Stoler MH,Wright TC Jr,Ferenczy A,Castle PE

更新日期：2014-06-01 00:00:00

abstract：:Vulvovaginal candidiasis (VVC) remains a common cause of morbidity, with three-quarters of women affected during their lifetimes. Use of antibiotics is an acknowledged trigger for VVC, which adversely affects women's physical and emotional health. Knowledge of patterns of genital Candida species-level identification i...

journal_title：Journal of clinical microbiology

authors： Pirotta MV,Garland SM

更新日期：2006-09-01 00:00:00

abstract：:Group B Streptococcus detection directly from Copan ESwab collected samples, using the BD Max GBS assay, was evaluated on receipt in the laboratory and after 24 h at room temperature. Results were compared to those using Lim broth enrichment PCR and culture. No significant difference was observed between 24 h ESwab an...

journal_title：Journal of clinical microbiology

authors： Silbert S,Rocchetti TT,Gostnell A,Kubasek C,Widen R

更新日期：2016-06-01 00:00:00

abstract：:Laboratory and clinical diagnostic classification of seropositive individuals, followed by treatment and supportive therapy, is an established component of Chagas' disease control in areas where this disease is endemic. However, most Chagas' disease patients live in remote areas where neither equipped laboratories nor...

journal_title：Journal of clinical microbiology

authors： Roddy P,Goiri J,Flevaud L,Palma PP,Morote S,Lima N,Villa L,Torrico F,Albajar-Viñas P

更新日期：2008-06-01 00:00:00

abstract：:Bacterial contamination frequently interferes with successful recovery of the Lyme spirochete from cultures of tissue from Borrelia burgdorferi-infected humans, rodents, or ticks. We used 0.20- and 0.45-microns-pore-size syringe-tip filters to recover spirochetes from cultures contaminated with other bacteria. Low con...

journal_title：Journal of clinical microbiology

authors： Jobe DA,Callister SM,Schell RF

更新日期：1993-07-01 00:00:00

abstract：:Since the introduction of antiviral compounds such as lamivudine and famciclovir in the treatment schedules of patients with chronic hepatitis B virus (HBV) infection, the accumulation of a variety of mutations in the HBV polymerase gene has been observed. The selection of these mutations is generally considered the c...

journal_title：Journal of clinical microbiology

authors： Stuyver L,Van Geyt C,De Gendt S,Van Reybroeck G,Zoulim F,Leroux-Roels G,Rossau R

更新日期：2000-02-01 00:00:00

abstract：:We compared a new colony hybridization assay with an established enzyme-linked immunosorbent assay for detection of enterotoxigenic Escherichia coli (ETEC) expressing colonization factor antigen I (CFA/I). The tests were applied to 135 human ETEC strains. Of these isolates, 30 had previously been characterized for CFA...

journal_title：Journal of clinical microbiology

authors： Grewal HM,Sommerfelt H,Gaastra W,Svennerholm AM,Bhan MK,Hamers AM,Kumar R,Wiklund G,Bjorvatn B

更新日期：1990-10-01 00:00:00