Comparative cost-effectiveness of two-tiered testing strategies for serodiagnosis of lyme disease with noncutaneous manifestations.

Abstract:

:The mainstay of laboratory diagnosis for Lyme disease is two-tiered serological testing, in which a reactive first-tier enzyme-linked immunosorbent assay (ELISA) or an immunofluorescence assay is supplemented by separate IgM and IgG immunoblots. Recent data suggest that the C6 ELISA can be substituted for immunoblots without a reduction in either sensitivity or specificity. In this study, the costs of 4 different two-tiered testing strategies for Lyme disease were compared using the median charges for these tests at 6 commercial diagnostic laboratories in 2012. The study found that a whole-cell sonicate ELISA followed by the C6 ELISA was the most cost-effective two-tiered testing strategy for Lyme disease with acute-phase serum samples. We conclude that the C6 ELISA can substitute for immunoblots in the two-tiered testing protocol for Lyme disease without a loss of sensitivity or specificity and is less expensive.

journal_name

J Clin Microbiol

authors

Wormser GP,Levin A,Soman S,Adenikinju O,Longo MV,Branda JA

doi

10.1128/JCM.01853-13

subject

Has Abstract

pub_date

2013-12-01 00:00:00

pages

4045-9

issue

12

eissn

0095-1137

issn

1098-660X

pii

JCM.01853-13

journal_volume

51

pub_type

杂志文章
  • Rapid and improved gas-liquid chromatography technique for detection of hippurate hydrolysis by Campylobacter jejuni and Campylobacter coli.

    abstract::A gas-liquid chromatographic method which requires no chloroform extraction of the split products has been investigated for the detection of hippurate hydrolysis by Campylobacter spp. This technique gave better reproducibility than other tests also used in this study and allows the routine use of the gas-liquid chroma...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.25.9.1776-1778.1987

    authors: Bär W,Fricke G

    更新日期:1987-09-01 00:00:00

  • Mycobacterium bovis Persistence in Two Different Captive Wild Animal Populations in Germany: a Longitudinal Molecular Epidemiological Study Revealing Pathogen Transmission by Whole-Genome Sequencing.

    abstract::Bovine tuberculosis (bTB) caused by Mycobacterium bovis is a transmissible disease notifiable to the World Organization for Animal Health and to the European Union, with ongoing efforts of surveillance and eradication in every EU member state. In Germany, a country which has been declared officially free from bovine t...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00302-18

    authors: Kohl TA,Utpatel C,Niemann S,Moser I

    更新日期:2018-08-27 00:00:00

  • Isolation and cultivation of Haemophilus ducreyi.

    abstract::A useful method for isolating and recognizing Haemophilus ducreyi from chancres and buboes of male patients is presented. A total of 41 clinical isolates of H. ducreyi were recovered from 33 patients over an 8-year period, and the experience with the 15 most recent isolates is presented in detail. Chocolate agar suppl...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.15.4.625-629.1982

    authors: Oberhofer TR,Back AE

    更新日期:1982-04-01 00:00:00

  • Validity of cultures of fluid collected through drainage catheters versus those obtained by direct aspiration.

    abstract::To examine the validity of cultures of fluid collected through drainage catheters, we reviewed retrospectively fluid specimens that had been collected through catheters in place for at least 2 days. These specimens were taken from patients at a large tertiary-care hospital. A total of 974 specimens representing 620 pa...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.39.1.66-68.2001

    authors: Everts RJ,Heneghan JP,Adholla PO,Reller LB

    更新日期:2001-01-01 00:00:00

  • The Panther Fusion System with Open Access Functionality for Laboratory-Developed Tests for Influenza A Virus Subtyping.

    abstract::Nucleic acid amplification tests, such as PCR, are the method of choice for respiratory virus testing, due to their superior diagnostic accuracy and fast turnaround time. The Panther Fusion (Fusion; Hologic) system has an array of highly sensitive in vitro diagnostic (IVD) real-time PCR assays for respiratory viruses,...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00188-20

    authors: Stellrecht KA,Cimino JL,Maceira VP

    更新日期:2020-05-26 00:00:00

  • Human papillomavirus genotyping using archival vulval dysplastic or neoplastic biopsy tissues: comparison between the INNO-LiPA and linear array assays.

    abstract::The Roche Linear Array (LA) and Innogenetics INNO-LiPA human papillomavirus (HPV) genotyping assays were compared for paraffin-embedded vulval tissues. The LA detected amplifiable DNA in 28 (57%) out of 49 biopsy specimens, 20 (40%) being HPV genotyped, compared to 49 (100%) and 41 (83%), respectively, detected by the...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02311-09

    authors: Tan SE,Garland SM,Rumbold AR,Tabrizi SN

    更新日期:2010-04-01 00:00:00

  • Influence of carbon dioxide on growth and antibiotic susceptibility of coagulase-negative staphylococci cultured in human peritoneal dialysate.

    abstract::Used peritoneal dialysis fluid was collected from patients undergoing continuous ambulatory peritoneal dialysis, and its pH and composition were assessed after incubation in either air or air with 5% CO2. Precipitation of calcium, magnesium, phosphate, and proteins occurred in the dialysis fluid incubated in air at 37...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.10.2183-2186.1990

    authors: Wilcox MH,Smith DG,Evans JA,Denyer SP,Finch RG,Williams P

    更新日期:1990-10-01 00:00:00

  • Successful Combination of Nucleic Acid Amplification Test Diagnostics and Targeted Deferred Neisseria gonorrhoeae Culture.

    abstract::Nucleic acid amplification tests (NAATs) are recommended for the diagnosis of N. gonorrhoeae infections because of their superior sensitivity. Increasing NAAT use causes a decline in crucial antimicrobial resistance (AMR) surveillance data, which rely on culture. We analyzed the suitability of the ESwab system for NAA...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00369-15

    authors: Wind CM,de Vries HJ,Schim van der Loeff MF,Unemo M,van Dam AP

    更新日期:2015-06-01 00:00:00

  • New Nocardia taxon among isolates of Nocardia brasiliensis associated with invasive disease.

    abstract::Nocardia brasiliensis, the second most frequently isolated aerobic actinomycete in the clinical laboratory, is usually associated with localized cutaneous infections. However, 22% of 238 N. brasiliensis isolates from the United States and 12% of 66 isolates from Queensland, Australia, which had been collected over a 1...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.6.1528-1533.1995

    authors: Wallace RJ Jr,Brown BA,Blacklock Z,Ulrich R,Jost K,Brown JM,McNeil MM,Onyi G,Steingrube VA,Gibson J

    更新日期:1995-06-01 00:00:00

  • Colorimetric method for rapid determination of bacteriuria.

    abstract::An inexpensive, rapid, and simple colorimetric test for detection of bacteriuria is described. This test does not require bacterial growth and has the marked advantage of being able to quantify bacteria, even when the organisms are present in the urine of bacteriuric patients who are being treated with antibiotics. Th...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.14.3.342-346.1981

    authors: Wallis C,Melnick JL,Longoria CJ

    更新日期:1981-09-01 00:00:00

  • Evaluation of the KOH test and the antibiotic disk test in routine clinical anaerobic bacteriology.

    abstract::We have evaluated the KOH test, the antibiotic disk identification test, and the Gram stain reaction for the preliminary grouping of gram-positive and gram-negative anaerobes and have assessed the value of erythromycin 60-micrograms-disk resistance as a predictive index of clindamycin resistance among anaerobes. By te...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.26.10.2144-2146.1988

    authors: Bourgault AM,Lamothe F

    更新日期:1988-10-01 00:00:00

  • Atypical toxin variant of Clostridium botulinum type B associated with infant botulism.

    abstract::An atypical toxin variant of Clostridium botulinum (strain 657) was isolated from the feces of a 6-week-old female infant whose symptoms and clinical history were consistent with infant botulism. Toxin detected in the feces and the toxin produced by isolates from the feces and from two rectal swabs could be neutralize...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.14.6.607-611.1981

    authors: Hatheway CL,McCroskey LM,Lombard GL,Dowell VR Jr

    更新日期:1981-12-01 00:00:00

  • Association of Bacteroides forsythus and a novel Bacteroides phylotype with periodontitis.

    abstract::Chronic periodontitis is a common infectious disease in the adult population. The etiology is clearly bacterial, and a small number of bacterial species have been consistently associated with periodontitis, including Bacteroides forsythus and Porphyromonas gingivalis. Comparatively little attention has been paid to th...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.40.3.821-825.2002

    authors: Leys EJ,Lyons SR,Moeschberger ML,Rumpf RW,Griffen AL

    更新日期:2002-03-01 00:00:00

  • Prospective cohort study of enterotoxigenic Escherichia coli infections in Argentinean children.

    abstract::In a follow-up study, enterotoxigenic Escherichia coli (ETEC) infections in 145 children from two communities located in northeastern Argentina were monitored for 2 years. The occurrence of diarrhea was monitored by weekly household visits. Of 730 fecal specimens collected, 137 (19%) corresponded to diarrheal episodes...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.9.2829-2833.1999

    authors: Viboud GI,Jouve MJ,Binsztein N,Vergara M,Rivas M,Quiroga M,Svennerholm AM

    更新日期:1999-09-01 00:00:00

  • Definition of a divergent epitope that allows differential detection of early protein p41 from human herpesvirus 6 variants A and B.

    abstract::The human herpesvirus 6 (HHV-6) early protein, p41, encoded by the U27 gene has been detected in oligodendrocytes of multiple sclerosis (MS) patients by using a monoclonal antibody (MAb to p41/38). We here report the antigenic epitope of HHV-6 p41 recognized by this MAb. First, we established that the MAb to p41/38 re...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.39.4.1449-1455.2001

    authors: Xu Y,Linde A,Dahl H,Winberg G

    更新日期:2001-04-01 00:00:00

  • Diagnostic assessment of an enzyme-linked immunosorbent assay for human and canine blastomycosis.

    abstract::An enzyme-linked immunosorbent assay (ELISA) for blastomycosis was evaluated with 65 human sera from culturally or histologically proven cases of blastomycosis, 53 sera from humans with heterologous infections, and 115 sera from apparently normal people. The diagnostic value of the ELISA was compared with that of the ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.23.2.294-297.1986

    authors: Turner S,Kaufman L,Jalbert M

    更新日期:1986-02-01 00:00:00

  • Evaluation of a point-of-care test, BVBlue, and clinical and laboratory criteria for diagnosis of bacterial vaginosis.

    abstract::Bacterial vaginosis (BV) remains the most common cause of abnormal vaginal discharge in women of reproductive age and is associated with increased susceptibility to human immunodeficiency virus and sexually transmitted infections and preterm delivery. Present diagnostic methods require access to microscopy and laborat...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.3.1304-1308.2005

    authors: Bradshaw CS,Morton AN,Garland SM,Horvath LB,Kuzevska I,Fairley CK

    更新日期:2005-03-01 00:00:00

  • Systematic review and meta-analysis of a urine-based pneumococcal antigen test for diagnosis of community-acquired pneumonia caused by Streptococcus pneumoniae.

    abstract::Standard culture methods for diagnosis of Streptococcus pneumoniae pneumonia take at least 24 h. The BinaxNOW urine-based test for S. pneumoniae (BinaxNOW-SP) takes only 15 min to conduct, potentially enabling earlier diagnosis and targeted treatment. This study was conducted to assess whether the use of BinaxNOW-SP a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,meta分析,评审

    doi:10.1128/JCM.00137-13

    authors: Sinclair A,Xie X,Teltscher M,Dendukuri N

    更新日期:2013-07-01 00:00:00

  • Irreproducible and uninterpretable Polymyxin B MICs for Enterobacter cloacae and Enterobacter aerogenes.

    abstract::Carbapenem-resistant Enterobacter species are emerging nosocomial pathogens. As with most multidrug-resistant Gram-negative pathogens, the polymyxins are often the only therapeutic option. In this study involving clinical isolates of E. cloacae and E. aerogenes, susceptibility testing methods with polymyxin B were ana...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02129-13

    authors: Landman D,Salamera J,Quale J

    更新日期:2013-12-01 00:00:00

  • U.S. case report of cerebral phaeohyphomycosis caused by Ramichloridium obovoideum (R. mackenziei): criteria for identification, therapy, and review of other known dematiaceous neurotropic taxa.

    abstract::We report a case of cerebral phaeohyphomycosis in a 36-year-old male caused by the neurotropic fungus Ramichloridium obovoideum (Matushima) de Hoog 1977 (Ramichloridium mackenziei Campbell et Al-Hedaithy 1993). This man resided in the Middle East, where the fungus appears to be endemic and, possibly, geographically re...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,评审

    doi:10.1128/JCM.36.3.708-715.1998

    authors: Sutton DA,Slifkin M,Yakulis R,Rinaldi MG

    更新日期:1998-03-01 00:00:00

  • Inactivation of mycoplasma in serum with binary ethyleneimine.

    abstract::A method of inactivating mycoplasma contaminants in sera by using binary ethyleneimine was tried. When used at the concentration recommended for inactivating viruses, 0.001 M, binary ethyleneimine inactivated only two of five mycoplasma species. Even at a concentration of 0.01 M, the compound did not consistently inac...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.11.4.377-379.1980

    authors: Christianson GG,Pemberton JR,Koski TA

    更新日期:1980-04-01 00:00:00

  • Veillonella parvula discitis and secondary bacteremia: a rare infection complicating endoscopy and colonoscopy?

    abstract::We report a case of Veillonella parvula lumbar discitis and secondary bacteremia confirmed by molecular characterization of the 16S rRNA genes. Identification of the organism was essential for an appropriate choice of antimicrobial therapy following the failure of empirical flucloxacillin. Veillonella spp. are normal ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,评审

    doi:10.1128/JCM.01633-06

    authors: Marriott D,Stark D,Harkness J

    更新日期:2007-02-01 00:00:00

  • Application of lipovitellin-salt-mannitol agar for screening, isolation, and presumptive identification of Staphylococcus aureus in a teaching hospital.

    abstract::Lipovitellin-salt-mannitol (LSM) plate medium was examined for its ability to directly isolate, recover, and presumptively identify Staphylococcus aureus from 418 clinical specimens. The criteria for medium evaluation included colony morphology reactions, selectivity, and ease of isolation. For 298 specimens used for ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.34.12.3012-3015.1996

    authors: Merlino J,Gill R,Robertson GJ

    更新日期:1996-12-01 00:00:00

  • Development of loop-mediated isothermal amplification assay for detection of Entamoeba histolytica.

    abstract::A novel one-step, closed-tube, loop-mediated isothermal amplification (LAMP) assay for detecting Entamoeba histolytica, one of the leading causes of morbidity in developing countries, was developed. The sensitivity of the LAMP assay is 1 parasite per reaction. A total of 130 clinical samples were analyzed, and the res...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00105-09

    authors: Liang SY,Chan YH,Hsia KT,Lee JL,Kuo MC,Hwa KY,Chan CW,Chiang TY,Chen JS,Wu FT,Ji DD

    更新日期:2009-06-01 00:00:00

  • Diagnosis of pneumococcal pneumonia by psaA PCR analysis of lung aspirates from adult patients in Kenya.

    abstract::psaA is the gene encoding pneumococcal surface adhesin A (PsaA), a 37-kDa protein expressed on the surface of Streptococcus pneumoniae. PCR primers for psaA have been shown to amplify the target DNA sequence in all 90 serotypes of S. pneumoniae and in none of 67 heterologous pathogens and colonizing bacteria of the up...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.6.2554-2559.2003

    authors: Scott JA,Marston EL,Hall AJ,Marsh K

    更新日期:2003-06-01 00:00:00

  • Characteristics of Bacteroides asaccharolyticus from dental plaques of beagle dogs.

    abstract::Gram-negative, non-saccharolytic, brown- or black-pigment-forming, nonmotile anaerboic coccobacilli, capable of decomposing hydrogen peroxide and identified as Bacteroides asaccharolyticus (B. melaninogenicus subsp. asaccharolyticus), were isolated from the supra- and subgingival plaques of beagle dogs with gingivitis...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.11.5.522-526.1980

    authors: Syed SA

    更新日期:1980-05-01 00:00:00

  • Neutralization of antimicrobial substances in new BacT/Alert FA and FN Plus blood culture bottles.

    abstract::Time to detection (TTD) in automated blood culture systems is delayed for sensitive microorganisms in the presence of antimicrobial substances and has been associated with worse outcomes for sepsis patients on inadequate empirical therapy. While resin addition removes antimicrobial substances to various degrees from b...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00103-13

    authors: Mitteregger D,Barousch W,Nehr M,Kundi M,Zeitlinger M,Makristathis A,Hirschl AM

    更新日期:2013-05-01 00:00:00

  • Escherichia fergusonii bacteremia in a diabetic patient with pancreatic cancer.

    abstract::Although Escherichia fergusonii has been identified for decades, it has rarely been recovered from clinical specimens and its clinical significance remains unclear. We describe a case of E. fergusonii bacteremia in a diabetic patient with pancreatic cancer. The isolate was confirmed by three commercial identification ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.05355-11

    authors: Lai CC,Cheng A,Huang YT,Chung KP,Lee MR,Liao CH,Hsueh PR

    更新日期:2011-11-01 00:00:00

  • Relationship between frequency of infectious human immunodeficiency virus type 1-harboring cells and kinetics of viral replication: a simple procedure for quantitation of infectious virus-carrying cells in blood samples.

    abstract::Statistical analysis of a limiting dilution assay (LDA) showed that the occurrence of infectious human immunodeficiency virus type 1 (HIV-1)-harboring cells in serially diluted samples of peripheral blood mononuclear cells (PBMCs) of HIV-1-seropositive patients fits the model describing a single-hit Poisson distributi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.10.2535-2538.1992

    authors: Lu W,Manolikakis G,Andrieu JM

    更新日期:1992-10-01 00:00:00

  • Validation of the SPF10 LiPA human papillomavirus typing assay using formalin-fixed paraffin-embedded cervical biopsy samples.

    abstract::Lower levels of performance of human papillomavirus (HPV) typing assays in studies using formalin-fixed paraffin-embedded (FFPE) tissue compared to those using exfoliated cervical cells have been reported. The interpretation of current studies is limited by bias in inclusion criteria, sample matching, and methods of c...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00286-09

    authors: Dal Bello B,Spinillo A,Alberizzi P,Cesari S,Gardella B,Silini EM

    更新日期:2009-07-01 00:00:00