Isolation and cultivation of Haemophilus ducreyi.

abstract：:Isolation of Burkholderia pseudomallei currently relies on the use of Ashdown's selective agar (ASA). We designed a new selective agar (Burkholderia pseudomallei selective agar [BPSA]) to improve recovery of the more easily inhibited strains of B. pseudomallei. B. pseudomallei, Burkholderia cepacia, and Pseudomonas ae...

journal_title：Journal of clinical microbiology

authors： Howard K,Inglis TJ

更新日期：2003-07-01 00:00:00

abstract：:We have used a polymerase chain reaction (PCR) to examine cervical cancer biopsy specimens and pelvic lymph nodes for the presence of human papillomavirus type 16 (HPV 16) DNA. Of the 75 cervical specimens tested, 36 (48%) were positive for HPV 16 in the PCR. A total of 65 pelvic lymph nodes removed during radical sur...

journal_title：Journal of clinical microbiology

authors： Czeglédy J,Póka R,Veress G,Gergely L

更新日期：1992-01-01 00:00:00

abstract：:The commercially available diagnostic tests for syphilis are mostly based on the use of extracted antigens of Treponema pallidum. Pronounced cross-reactivities with other spirochete antigens are often reported. The aim of this study was to validate a novel multiparametric assay (the assay performed with the kit) INNO-...

journal_title：Journal of clinical microbiology

authors： Ebel A,Vanneste L,Cardinaels M,Sablon E,Samson I,De Bosschere K,Hulstaert F,Zrein M

更新日期：2000-01-01 00:00:00

abstract：:In the era after the introduction of the meningococcal serogroup C conjugate vaccine, from 1 January 2003 to 31 December 2010, serogroup B meningococci were the major cause of invasive meningococcal disease in the province of Québec, Canada, being responsible for 72% of all meningococcal disease cases. Of the 334 inva...

journal_title：Journal of clinical microbiology

authors： Zhou J,Lefebvre B,Deng S,Gilca R,Deceuninck G,Law DK,De Wals P,Tsang RS

更新日期：2012-05-01 00:00:00

abstract：:Acrophialophora fusispora is an emerging opportunistic fungus capable of causing human infections. The taxonomy of the genus is not yet resolved and, in order to facilitate identification of clinical specimens, we have studied a set of clinical and environmental Acrophialophora isolates by morphological and molecular ...

journal_title：Journal of clinical microbiology

authors： Sandoval-Denis M,Gené J,Sutton DA,Wiederhold NP,Guarro J

更新日期：2015-05-01 00:00:00

abstract：:A total of 309 Helicobacter pylori isolates from 18 different countries were analyzed with a previously developed lectin typing system. The system was developed by using a proteolytic pretreatment to enhance the carbohydrate fraction of the sample. Four lectins from Ulex europaeus, Lotus tetragonolobus, Erythrina cris...

journal_title：Journal of clinical microbiology

authors： Hynes SO,Broutet N,Wadström T,Mikelsaar M,O'Toole PW,Telford J,Engstrand L,Kamiya S,Mentis AF,Moran AP,Eurohepygast Study Group.

更新日期：2002-01-01 00:00:00

abstract：:The usefulness of randomly amplified polymorphic DNA method (RAPD) to identify each species of genus Erysipelothrix and for epidemiological analysis of this genus was studied. Eighty-one strains and 18 random primers were tested. Among the tested primers, the primers NK51 (GGTGGTGGTATC) and NK6 (CCCGCGCCCC) produced n...

journal_title：Journal of clinical microbiology

authors： Okatani AT,Hayashidani H,Takahashi T,Taniguchi T,Ogawa M,Kaneko K

更新日期：2000-12-01 00:00:00

abstract：:We validated a commercial enzyme-linked immunosorbent assay for the detection of anti-CagA antibodies in Brazilian patients with Helicobacter pylori infection. The test presented high sensitivity (97.4%) and specificity (88.9%) when employed in patients without gastric carcinoma. However, in gastric carcinoma patients...

journal_title：Journal of clinical microbiology

authors： Rocha AM,Rocha GA,Santos A,de Oliveira CA,Queiroz DM

更新日期：2003-01-01 00:00:00

abstract：:Four hundred thirty-two lactose-negative colonies isolated from human feces on stool differential agar media were flooded with one drop of MUCAP Test reagent (Biolife Italiana S.r.I., Milan, Italy) and observed under a Wood lamp for the development of a blue fluorescence over or around the colony. On the basis of manu...

journal_title：Journal of clinical microbiology

authors： Aguirre PM,Cacho JB,Folgueira L,López M,García J,Velasco AC

更新日期：1990-01-01 00:00:00

abstract：:In a collaborative study at three university hospitals, the recovery of microorganisms and the speed of detection of microbial growth by the BacT/Alert (Organon Teknika Corporation, Durham, N.C.) and BACTEC 660/730 (Becton-Dickinson Diagnostic Instrument Systems, Sparks, Md.) nonradiometric blood culture systems were ...

journal_title：Journal of clinical microbiology

authors： Wilson ML,Weinstein MP,Reimer LG,Mirrett S,Reller LB

更新日期：1992-02-01 00:00:00

abstract：:Salmonella enterica serovar Senftenberg is a common nontyphoidal Salmonella serotype which causes human Salmonella infections worldwide. In this study, 182 S. Senftenberg isolates, including 17 atypical non-hydrogen sulfide (H2S)-producing isolates, were detected in China from 2005 to 2011. The microbiological and gen...

journal_title：Journal of clinical microbiology

authors： Yi S,Xie J,Liu N,Li P,Xu X,Li H,Sun J,Wang J,Liang B,Yang C,Wang X,Hao R,Wang L,Wu Z,Zhang J,Wang Y,Huang L,Sun Y,Klena JD,Meng J,Qiu S,Song H

更新日期：2014-07-01 00:00:00

abstract：:Flagellar (H) antigens are mostly encoded by genes at the fliC locus in E. coli. We have sequenced 11 H7 fliC genes from Escherichia coli strains that belong to seven O serotypes. These sequences, together with those of nine other H7 fliC genes (from strains of three different O serotypes) sequenced recently (S. D. Re...

journal_title：Journal of clinical microbiology

authors： Wang L,Rothemund D,Curd H,Reeves PR

更新日期：2000-05-01 00:00:00

abstract：:Recent studies have speculated on the possible role of the mother in transmitting Helicobacter pylori infection to their children. In an attempt to either prove or disprove this supposition, we investigated the rates of infection of children born to H. pylori-positive mothers from birth to 5 years of age using serolog...

journal_title：Journal of clinical microbiology

authors： Konno M,Fujii N,Yokota S,Sato K,Takahashi M,Sato K,Mino E,Sugiyama T

更新日期：2005-05-01 00:00:00

abstract：:In sera from 4 of 25,000 individuals tested for antibodies against streptococci, extremely high antideoxyribonuclease B titers ( > 10(6) U/ml) were found. Two of the cases were diagnosed as monoclonal gammopathies. The M-components were shown to possess the anti-deoxyribonuclease B activity. The other two cases were d...

journal_title：Journal of clinical microbiology

authors： Hederstedt B,Holm SE,Norberg R

更新日期：1980-06-01 00:00:00

abstract：:A case of pyogenic liver abscess (PLA) due to Rhodococcus equi in an immunocompetent individual was successfully treated by combining surgery and antibiotics. The R. equi-targeted antimicrobial agents erythromycin and rifampin were used only after surgical resection of the lesion and identification of the infective or...

journal_title：Journal of clinical microbiology

authors： Napoleão F,Damasco PV,Camello TC,do Vale MD,de Andrade AF,Hirata R Jr,de Mattos-Guaraldi AL

更新日期：2005-02-01 00:00:00

abstract：:One of the major unanswered questions regarding the presence of Listeria monocytogenes in foods is how many cells must be ingested in order to cause illness. To answer this question, studies were undertaken by using Macaca fascicularis (cynomolgus monkey) as an animal model. Healthy nonhuman primates were dosed with v...

journal_title：Journal of clinical microbiology

authors： Farber JM,Daley E,Coates F,Beausoleil N,Fournier J

更新日期：1991-11-01 00:00:00

abstract：:Between 1999 and 2002, 496 invasive group A streptococcal (GAS) isolates from clinical microbiological departments in Denmark and subsequently 487 (98%) questionnaires from the clinicians treating the patients were received as part of a national surveillance. emm types and streptococcal superantigen (SAg) genes were d...

journal_title：Journal of clinical microbiology

authors： Ekelund K,Skinhøj P,Madsen J,Konradsen HB

更新日期：2005-04-01 00:00:00

abstract：:A large pool of refined endotoxin was prepared from Escherichia coli O113 by extraction with hot acqueous phenol. It was characterized chemically and biologically and will be available for a reference standard designated as reference endotoxin EC. ...

journal_title：Journal of clinical microbiology

authors： Rudbach JA,Akiya FI,Elin RJ,Hochstein HD,Luoma MK,Milner EC,Milner KC,Thomas KR

更新日期：1976-01-01 00:00:00

abstract：:A solid-phase enzyme immunoassay (EIA) was developed for the detection of poliovirus antigen. Rabbit and guinea pig antisera for the assay were raised against purified poliovirus type 3/Fin (strain 3/Fin/K) isolated from a fecal specimen from a meningitis patient during an outbreak of poliomyelitis in Finland in 1984....

journal_title：Journal of clinical microbiology

authors： Ukkonen P,Huovilainen A,Hovi T

更新日期：1986-12-01 00:00:00

abstract：:The detection of porphyrins produced from omega-aminolevulinic acid was evaluated for use in speciation of the Haemophilus. Two hundred and forty-eight clinical isolates of nonhemolytic Haemophilus were tested concurrently for porphyrin production and for X and V requirements. The porphyrin production test was more ra...

journal_title：Journal of clinical microbiology

authors： Lund ME,Blazevic DJ

更新日期：1977-02-01 00:00:00

abstract：:The purpose of this study was to determine whether individual milk samples can replace serum samples for the detection of bovine herpesvirus 1 (BHV1) glycoprotein E (gE)-specific antibodies. Serum and milk samples were collected at the same time from cattle in BHV1-free herds, cattle in unvaccinated herds, and cattle ...

journal_title：Journal of clinical microbiology

authors： Wellenberg GJ,Verstraten ER,Mars MH,Van Oirschot JT

更新日期：1998-02-01 00:00:00

abstract：:The emergence of ciprofloxacin-resistant Salmonella enterica serovar Choleraesuis in recent years has become an important public health issue in Taiwan. The resistant strains that cause human infections are considered to be from pigs. In this study, we characterized 157 swine and 42 human Salmonella serovar Choleraesu...

journal_title：Journal of clinical microbiology

authors： Chang CC,Lin YH,Chang CF,Yeh KS,Chiu CH,Chu C,Chien MS,Hsu YM,Tsai LS,Chiou CS

更新日期：2005-06-01 00:00:00

abstract：:Monoclonal antibodies to Staphylococcus aureus capsular polysaccharide types 5 and 8 were used in an enzyme-linked immunosorbent assay to serotype 74 and 42 coagulase-negative isolates from cow and goat milk, respectively. Eighteen (15.5%) isolates were typable: 13 Staphylococcus haemolyticus, 1 S. hyicus, 1 S. simula...

journal_title：Journal of clinical microbiology

authors： Poutrel B,Mendolia C,Sutra L,Fournier JM

更新日期：1990-02-01 00:00:00

abstract：:Histoplasmosis is an important systemic fungal infection, particularly among immunocompromised individuals, who may develop a progressive disseminated form which is often fatal if it is untreated. In such patients, the detection of antibody responses for both diagnosis and follow-up may be of limited use, whereas the ...

journal_title：Journal of clinical microbiology

authors： Gómez BL,Figueroa JI,Hamilton AJ,Diez S,Rojas M,Tobón A,Restrepo A,Hay RJ

更新日期：1999-03-01 00:00:00

abstract：:Potomac horse fever, a recently recognized disease of equines, characterized by high fever, leukopenia, and a profuse diarrhea, was studied for its etiology. An Ehrlichia organism was isolated in equine macrophage-fibroblast cell cultures and mouse macrophage cell cultures from the mononuclear cells of blood of infect...

journal_title：Journal of clinical microbiology

authors： Dutta SK,Myrup AC,Rice RM,Robl MG,Hammond RC

更新日期：1985-08-01 00:00:00

abstract：:A total of 101 food-borne and waterborne outbreaks that were caused by norovirus and that resulted in more than 4,100 cases of illness were reported to the Swedish Institute for Infectious Disease Control from January 2002 to December 2006. Sequence and epidemiological data for isolates from 73 outbreaks were analyzed...

journal_title：Journal of clinical microbiology

authors： Lysén M,Thorhagen M,Brytting M,Hjertqvist M,Andersson Y,Hedlund KO

更新日期：2009-08-01 00:00:00

abstract：:Sequence-based typing (SBT), analogous to multilocus sequence typing (MLST), is the current "gold standard" typing method for investigation of legionellosis outbreaks caused by Legionella pneumophila However, as common sequence types (STs) cause many infections, some investigations remain unresolved. In this study, va...

journal_title：Journal of clinical microbiology

authors： David S,Mentasti M,Tewolde R,Aslett M,Harris SR,Afshar B,Underwood A,Fry NK,Parkhill J,Harrison TG

更新日期：2016-08-01 00:00:00

abstract：:The disease spectrum associated with human bocavirus-1 infection remains to be fully defined. We report a case of bocavirus-1-associated bronchiolitis, leading to severe respiratory failure and extracorporeal membrane oxygenation in a 4-year-old child, and suggest blood testing for human bocavirus-1 in children with s...

journal_title：Journal of clinical microbiology

authors： Edner N,Castillo-Rodas P,Falk L,Hedman K,Söderlund-Venermo M,Allander T

更新日期：2012-02-01 00:00:00

abstract：:Serogrouping of Bacteroides nodosus is based on antigenic differences in fimbriae of the different New Zealand prototype strains. Because of the time needed to isolate and grow pure cultures of B. nodosus and the difficulty in distinguishing between different serogroups because of cross-agglutination, a new DNA-based ...

journal_title：Journal of clinical microbiology

authors： John GH,Carlson JO,Kimberling CV,Ellis RP

更新日期：1990-11-01 00:00:00

abstract：:Vaginal discharge specimens from 105 women were examined by wet mount, culture, and a new direct immunofluorescence assay to detect Trichomonas vaginalis. The organism was detected by culture in 31 patients, by direct immunofluorescence in 26 patients, and by wet mount in 21 patients. ...

journal_title：Journal of clinical microbiology

authors： Smith RF

更新日期：1986-12-01 00:00:00