Abstract:
:Flagellar (H) antigens are mostly encoded by genes at the fliC locus in E. coli. We have sequenced 11 H7 fliC genes from Escherichia coli strains that belong to seven O serotypes. These sequences, together with those of nine other H7 fliC genes (from strains of three different O serotypes) sequenced recently (S. D. Reid, R. K. Selander, and T. S. Whittam, J. Bacteriol. 181:153-160, 1999), include 10 different sequences. The differences between these 10 sequences range from 0.06 to 3.12%. By comparison with other E. coli flagellin genes, we have identified primer length sequences specific for H7 genes in general and others specific for H7 genes of O157 and O55 strains: the specificity was confirmed by PCR testing the type strains for all 53 E. coli H types. We have previously identified genes specific for the E. coli O157 antigen, and use of the combination of O157- and H7-specific primers allows the sensitive and rapid detection of O157:H7 E. coli strains, which cause the majority of hemorrhagic colitis cases.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Wang L,Rothemund D,Curd H,Reeves PRdoi
10.1128/JCM.38.5.1786-1790.2000subject
Has Abstractpub_date
2000-05-01 00:00:00pages
1786-90issue
5eissn
0095-1137issn
1098-660Xjournal_volume
38pub_type
杂志文章abstract::We have developed a hexaplex PCR assay for rapid detection of the virulence and regulatory genes for cholera toxin enzymatic subunit A (ctxA), zonula occludens toxin (zot), accessory cholera enterotoxin (ace), toxin-coregulated pilus (tcpA), outer membrane protein (ompU), and central regulatory protein ToxR (toxR) in ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.40.11.4321-4324.2002
更新日期:2002-11-01 00:00:00
abstract::This is the first reported case of endocarditis due to the Lactobacillus-like vancomycin-resistant gram-positive bacillus Weissella confusa. Full identification and susceptibility testing of Lactobacillus-like organisms recovered in blood culture should be performed for patients with clinical presentations that sugges...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.41.5.2237-2239.2003
更新日期:2003-05-01 00:00:00
abstract::Shortly after adopting a 6-week-old cat, a veterinarian was bitten on the left index finger. Within 3 weeks, he developed headache, fever, and left axillary lymphadenopathy. Initial blood cultures from the cat and veterinarian were sterile. Repeat cultures from the cat grew Bartonella-like organisms with lophotrichous...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.35.7.1813-1818.1997
更新日期:1997-07-01 00:00:00
abstract::Antibodies to Japanese encephalitis virus (JEV) nonstructural 1 (NS1) protein constitute a marker of natural JEV infection among populations vaccinated with inactivated JE vaccine. In Japan, with few recent human JE cases, the natural infection rate is critical to evaluate the necessity of continuing JE vaccination. A...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.42.11.5087-5093.2004
更新日期:2004-11-01 00:00:00
abstract::A search has been made for corynebacterial phospholipase D, "ovis toxin," a sphingomyelinase (phosphatidylcholine phosphohydrolase, EC 3.1.4.4), among a wide variety of corynebacteria. Phospholipase D activity has been found in strains exhibiting the biochemical properties characteristic of Corynebacterium pseudotuber...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.13.2.335-343.1981
更新日期:1981-02-01 00:00:00
abstract::A new case of Campylobacter hyointestinalis-associated diarrhea in a human is reported. The medical history of the patient was significant for immunodeficiency because of an evolutive chronic myeloid leukemia. The diarrhea rapidly stopped after administration of oral erythromycin. No other enteropathogenic agent was f...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.26.12.2659-2660.1988
更新日期:1988-12-01 00:00:00
abstract::A panel (ENVA-1) of well-defined blinded samples containing wild-type and mutant human immunodeficiency virus type 1 (HIV-1) reverse transcriptase was analyzed by automated DNA sequencing in 23 laboratories worldwide. Drug resistance mutations at codons 41, 215, and 184 were present in the panel samples at different r...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,多中心研究
doi:10.1128/JCM.37.7.2291-2296.1999
更新日期:1999-07-01 00:00:00
abstract::Enterobacter cloacae strain G6809 with reduced susceptibility to carbapenems was identified from a patient in a long-term acute care hospital in Kentucky. G6809 belonged to sequence type (ST) 88 and carried two carbapenemase genes, bla(KPC-18) and bla(VIM-1). Whole-genome sequencing localized bla(KPC-18) to the chromo...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02739-15
更新日期:2016-03-01 00:00:00
abstract::The standard complement fixation (CF) test, a commercial agarose gel immunodiffusion (AGID) test (ImmuCell Corporation, Portland, Maine), and two commercial enzyme-linked immunosorbent assays (ELISAs; Allied Laboratories, Glenwood Springs, Colo. [Allied ELISA], and the CSL, Limited, [Parkville, Victoria, Australia] en...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.30.5.1134-1139.1992
更新日期:1992-05-01 00:00:00
abstract::A neutralizing monoclonal antibody, B37:1, against a human rotavirus (B37) with a "super-short" RNA pattern was derived. The antibody gave serotype-specific reactions with a panel of standard rotaviruses and was reactive in an enzyme immunoassay. Epidemiological studies of this new human serotype should now be possibl...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.25.12.2426-2427.1987
更新日期:1987-12-01 00:00:00
abstract::Twenty-nine Mycobacterium reference strains representing 10 species and 60 mycobacterial cultures isolated from sputum specimens were studied. These cultures were grown in Bactec 7H12B medium (Becton Dickinson and Co., Paramus, N.J.) supplemented with oleic acid-albumin-dextrose-catalase enrichment broth (Becton Dicki...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.34.5.1317-1320.1996
更新日期:1996-05-01 00:00:00
abstract::This report describes the results of an 11-laboratory study to determine if a cefoxitin broth microdilution MIC test could predict the presence of mecA in staphylococci. Using breakpoints of < or = 4 microg/ml for mecA-negative and > or = 6 or 8 microg/ml for mecA-positive isolates, sensitivity and specificity based o...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,多中心研究
doi:10.1128/JCM.02304-08
更新日期:2009-06-01 00:00:00
abstract::In this report we present clinical descriptions of 12 Hawaiian patients from whom Escherichia vulneris or E. hermannii strains were isolated. All but two patients had soft-tissue infections with multiple bacteria, particularly Staphylococcus aureus. The other two had purulent conjunctivitis associated with S. aureus a...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.22.2.283-285.1985
更新日期:1985-08-01 00:00:00
abstract::From August 1996 to May 1997, six verotoxin-producing Escherichia coli (VTEC) strains were isolated from stool specimens of adults suffering from hemolytic-uremic syndrome (HUS). All the isolates were stx2 positive and belonged to different serotypes: O6:H4, O91:H10, O91:H21, O rough:H16, OX3:H-, and O nontypeable: H-...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.36.6.1777-1780.1998
更新日期:1998-06-01 00:00:00
abstract::A new DNA probe assay (INNO LiPA Mycobacteria; Innogenetics, Ghent, Belgium) for the simultaneous identification, by means of reverse hybridization and line-probe technology, of Mycobacterium tuberculosis complex, Mycobacterium kansasii, Mycobacterium xenopi, Mycobacterium gordonae, the species of the Mycobacterium av...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.39.3.1079-1084.2001
更新日期:2001-03-01 00:00:00
abstract::We tested 606 specimens for cytomegalovirus infection. Fifty-two specimens were positive by conventional culture versus forty-three by pre-cytopathic effect detection. Twenty-nine specimens were positive by both methods. Because of toxicity, specimens should be set up on both conventional and shell vial culture system...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.4.806-807.1990
更新日期:1990-04-01 00:00:00
abstract::For recovery of brucellae from clinical specimens, a lysis-concentration procedure was superior to the procedure of Castañeda (M. R. Castañeda, Proc. Soc. Exp. Biol. Med. 64:114-115, 1947). ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.20.3.586-.1984
更新日期:1984-09-01 00:00:00
abstract::Escherichia coli is the most common cause of human and canine urinary tract infection (UTI). Clonal groups, often with high levels of antimicrobial resistance, are a major component of the E. coli population that causes human UTI. While little is known about the population structure of E. coli that causes UTI in dogs,...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,多中心研究
doi:10.1128/JCM.00788-18
更新日期:2018-08-27 00:00:00
abstract::Hepatitis G virus (HGV) was recently identified as a new member of the Flaviviridae, but its clinical significance is still unclear. Since no immunoassay for the diagnosis of HGV is available, we developed a sensitive reverse transcription-PCR (RT-PCR) assay to facilitate the detection of the viral genome by mass scre...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.34.11.2660-2664.1996
更新日期:1996-11-01 00:00:00
abstract::PCR was performed for the detection of Helicobacter pylori in feces from 24 patients with proven infections. Several precautions were taken to overcome possible inhibition of PCR with feces. In the first 12 patients, feces were examined shortly after endoscopy. In another group of 12 patients, who were treated during ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.32.5.1346-1348.1994
更新日期:1994-05-01 00:00:00
abstract::The Scottish Meningococcus and Pneumococcus Reference Laboratory (SMPRL) provides a national service for the laboratory confirmation of meningococcal and pneumococcal disease in Scotland. Part of this service includes the serogrouping of meningococcal isolates followed by typing and subtyping. The procedures for this ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.39.9.3066-3071.2001
更新日期:2001-09-01 00:00:00
abstract::The objective of the present study was the development of a diagnostic reverse transcription (RT)-PCR for the specific detection of enterovirus (EV) RNA in clinical specimens controlled by an internal control (IC) RNA. The IC RNA contains the same primer binding sites as EV RNA but has a different probe region. The IC...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.42.7.3059-3064.2004
更新日期:2004-07-01 00:00:00
abstract::Clostridium hathewayi is a newly described Clostridium species isolated from the feces of healthy human individuals, but its clinical significance is not known. We describe a case of human infection associated with C. hathewayi. The bacterium (strain HKU18) was isolated from the blood culture of a 39-year-old patient ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.42.12.5947-5949.2004
更新日期:2004-12-01 00:00:00
abstract::Because strains of Staphylococcus aureus that are resistant to penicillinase-resistant penicillins may be difficult to detect in the clinical laboratory, a variety of changes in methodology have been suggested to increase their detection. In 1984, the West Los Angeles Veterans Administration Medical Center experienced...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.25.5.840-844.1987
更新日期:1987-05-01 00:00:00
abstract::A total of 189 Candida albicans isolates have been typed by multilocus enzyme electrophoresis. The results obtained confirm the clonal mode of reproduction of C. albicans. The C. albicans populations found in the oropharynx of human immunodeficiency virus (HIV)-infected patients, in the oropharynx of healthy carriers,...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.34.5.1235-1248.1996
更新日期:1996-05-01 00:00:00
abstract::Broad-spectrum analysis for pathogens in patients with respiratory tract infections is becoming more relevant as the number of potential infectious agents is still increasing. Here we describe the new multiparameter RespiFinder assay, which is based on the multiplex ligation-dependent probe amplification (MLPA) techno...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02294-07
更新日期:2008-04-01 00:00:00
abstract::In specific-pathogen-free dogs experimentally infected with Borrelia burgdorferi by tick exposure, treatment with high doses of amoxicillin or doxycycline for 30 days diminished but failed to eliminate persistent infection. Although joint disease was prevented or cured in five of five amoxicillin- and five of six doxy...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.35.1.111-116.1997
更新日期:1997-01-01 00:00:00
abstract::A human astrovirus (HAstV) strain from Kenya was characterized by nucleotide sequence analysis. Sequences from open reading frame 1a (ORF1a) clustered with genotype 6/7, those from ORF1b clustered with genotype 3, and those from ORF2 clustered with genotype 2. A recombination point in the ORF1b-ORF2 junction was ident...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01093-10
更新日期:2011-02-01 00:00:00
abstract::The aims of this study were to compare a novel PCR-based Chlamydia trachomatis detection and genotyping (Ct-DT) assay with the FDA-approved, commercially available C. trachomatis detection Hybrid Capture 2 (HC2) assay and to investigate the C. trachomatis serovar distribution among young women in a rural Costa Rican s...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01155-07
更新日期:2007-12-01 00:00:00
abstract::The factors that regulate cytomegalovirus (CMV) excretion from the genitourinary tract are poorly understood. To assess the role of cell-mediated immunity in such excretion, a CMV-specific mononuclear blastogenesis assay was used to study a predominantly lower-socioeconomic-status population of 92 healthy nonpregnant ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.17.4.582-587.1983
更新日期:1983-04-01 00:00:00