Abstract:
:The p44 gene of the agent of human granulocytic ehrlichiosis (aoHGE) encodes a 44-kDa major outer surface protein. A technique was developed for the typing of the aoHGE based on the PCR amplification of the p44 gene followed by a multiple restriction digest with HindIII, EcoRV, and AspI to generate restriction fragment length polymorphism patterns. Twenty-four samples of the aoHGE were collected from geographically dispersed sites in the United States and included isolates from humans, equines, canines, small mammals, and ticks. Six granulocytic ehrlichiosis (GE) types were identified. The GE typing method is relatively simple to perform, is reproducible, and is able to differentiate among the various isolates of granulocytic ehrlichiae in the United States. These characteristics suggest that this GE typing method may be an important epizootiological and epidemiological tool.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Carter SE,Ravyn MD,Xu Y,Johnson RCdoi
10.1128/jcm.39.9.3398-3401.2001subject
Has Abstractpub_date
2001-09-01 00:00:00pages
3398-401issue
9eissn
0095-1137issn
1098-660Xjournal_volume
39pub_type
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