Sequencing-based genotyping of mixed human papillomavirus infections by use of RipSeq software.

abstract：:The Binax legionella urinary antigen (LUA) enzyme immunoassay (Binax, Portland, Maine) was evaluated in 159 patients with suspected or proven legionellosis and 209 controls. A positive LUA test was found in 37% of patients with suspected legionellosis overall and in 83% of those with proven Legionella pneumophila sero...

journal_title：Journal of clinical microbiology

authors： Kazandjian D,Chiew R,Gilbert GL

更新日期：1997-04-01 00:00:00

abstract：:A total of 49 adenovirus type 3 (Ad3) strains from patients with epidemic keratoconjunctivitis at an eye clinic in Sapporo, Japan, from 1983 to 1986 were classified into either genome type Ad3f (26 strains) or Ad3g (23 strains). By the use of Hinf1 and TaqI restriction endonucleases, they were classified into 11 and 3...

journal_title：Journal of clinical microbiology

authors： Itakura S,Aoki K,Sawada H,Shinagawa M

更新日期：1990-10-01 00:00:00

abstract：:Point source norovirus outbreaks can be difficult to track due to high background levels of the virus in the environment and the limited strain variation in some genotyping regions. However, rapid and accurate source identification can limit the spread of a foodborne outbreak and reduce the number of cases. Harmonizat...

journal_title：Journal of clinical microbiology

authors： Mattison K,Grudeski E,Auk B,Charest H,Drews SJ,Fritzinger A,Gregoricus N,Hayward S,Houde A,Lee BE,Pang XL,Wong J,Booth TF,Vinjé J

更新日期：2009-12-01 00:00:00

abstract：:Standardization of sequence chromatogram analysis is required for consistent genotypic tropism determination across laboratories. A freely available, fast, and automated chromatogram analysis tool (RECall) provided tropism interpretations equivalent to those of manual sequence editing of 521 V3 loop HIV-1 population s...

journal_title：Journal of clinical microbiology

authors： Pou C,Bellido R,Casadellà M,Puig T,Clotet B,Harrigan R,Paredes R

更新日期：2013-08-01 00:00:00

abstract：:Fluorescence of sensitized bacterial suspensions, reactive with either fluorescein-labeled or rhodamine-labeled antiglobulins, could be quantitatively distinguished in dual-labeled preparations by fluorescence immunoassay. ...

journal_title：Journal of clinical microbiology

authors： Gillis TP,Thompson JJ

更新日期：1978-09-01 00:00:00

abstract：:Two colorimetric methods that use Alamar Blue or 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) for assaying the in vitro activities of antifungal agents have been described. We report that both tests performed similarly when the antifungal activity of amphotericin B against Candida albicans wa...

journal_title：Journal of clinical microbiology

authors： Jahn B,Stüben A,Bhakdi S

更新日期：1996-08-01 00:00:00

abstract：:A multiplex PCR (mPCR) protocol was developed for simultaneous detection of the gyrB gene in Streptococcus pneumoniae, Streptococcus mitis, and Streptococcus oralis, and the specificity was evaluated using 141 coccus strains. Genomic DNAs purified from S. pneumoniae, S. mitis, and S. oralis strains were efficiently de...

journal_title：Journal of clinical microbiology

authors： Kim W,Park HK,Hwang WJ,Shin HS

更新日期：2013-03-01 00:00:00

abstract：:In October 2001, the first disseminated biological warfare attack was perpetrated on American soil. Initially, a few clinical microbiology laboratories were testing specimens from acutely ill patients and also being asked to test nasal swabs from the potentially exposed. Soon after, a significant number of clinical mi...

journal_title：Journal of clinical microbiology

authors： Craft DW,Lee PA,Rowlinson MC

更新日期：2014-07-01 00:00:00

abstract：:The DNA hybridization assay for genes encoding for Escherichia coli enterotoxins was used to examine water specimens in Thailand. In a reconstruction experiment, the DNA hybridization assay was 10(4) times more sensitive than testing random E. coli in the Y-1 adrenal and suckling mouse assays in identifying enterotoxi...

journal_title：Journal of clinical microbiology

authors： Echeverria P,Seriwatana J,Chityothin O,Chaicumpa W,Tirapat C

更新日期：1982-12-01 00:00:00

abstract：:A fastidious, gram-negative bacterium was isolated from the blood of a 51-year-old man who had acute infectious endocarditis (IE). Characterization of the organism through phenotypic and genotypic analyses revealed the causative role of Cardiobacterium valvarum. This is the third reported case of IE caused by C. valva...

journal_title：Journal of clinical microbiology

authors： Bothelo E,Gouriet F,Fournier PE,Roux V,Habib G,Thuny F,Metras D,Raoult D,Casalta JP

更新日期：2006-02-01 00:00:00

abstract：:A double-antibody (sandwich) enzyme-linked immunosorbent assay (ELISA) was adapted to detect Rift Valley fever virus antigen. Antibodies were purified from hyperimmune mouse and rabbit sera by affinity chromatography, using CNBr-activated Sepharose 4B coupled to a beta-propiolactone-inactivated sucrose-acetone-extract...

journal_title：Journal of clinical microbiology

authors： Niklasson B,Grandien M,Peters CJ,Gargan TP 2nd

更新日期：1983-06-01 00:00:00

abstract：:Diagnosis of invasive aspergillosis (IA) requires increasingly rapid molecular methods that enable sensitive detection and discrimination between species. We designed and evaluated a real-time PCR-based method that combined melting temperature (T(m)) calling analysis of a specific probe with high-resolution melting an...

journal_title：Journal of clinical microbiology

authors： Alonso M,Escribano P,Guinea J,Recio S,Simon A,Peláez T,Bouza E,García de Viedma D

更新日期：2012-10-01 00:00:00

abstract：:Tissue cultures established from brain tissues of six patients with various chronic, degenerative diseases did not show evidence of viral agents. Sucrose gradient analysis of radioactively labeled culture fluid from two patients failed to reveal the presence of any incomplete or defective virus. Extracellular fluids f...

journal_title：Journal of clinical microbiology

authors： Lennette ET

更新日期：1975-05-01 00:00:00

abstract：:Two brands of thiosulfate citrate bile salts sucrose agar and Monsur taurocholate tellurite gelatin (TTG) agar were compared with two newly developed media, sucrose tellurite teepol agar and Vibrio parahaemolyticus agar for isolation and identification of Vibrio cholerae. The thiosulfate citrate bile salts sucrose and...

journal_title：Journal of clinical microbiology

authors： Morris GK,Merson MH,Huq I,Kibrya AK,Black R

更新日期：1979-01-01 00:00:00

abstract：:Several studies have identified thymidine excess in susceptibility test media as the cause of spurious resistance of various bacteria to sulfamethoxazole-trimethoprim. We document the phenomenon in Salmonella typhi and Salmonella paratyphi-A and demonstrate its occurrence in 3 of 17 (18%) lots of Mueller-Hinton agars ...

journal_title：Journal of clinical microbiology

authors： Escamilla J,Kilpatrick ME,Ugarte HF

更新日期：1986-01-01 00:00:00

abstract：:An in situ DNA hybridization kit for cytomegalovirus (CMV) was evaluated for the detection of CMV in centrifugation culture. Of 61 clinical specimens, 17 (27.8%) were positive for CMV by monoclonal antibody staining following centrifugation. Of the 17 positive specimens, 15 were detected by DNA hybridization (24.5%). ...

journal_title：Journal of clinical microbiology

authors： Gleaves CA,Hursh DA,Rice DH,Meyers JD

更新日期：1989-01-01 00:00:00

abstract：:Nosocomial oxacillin-resistant Staphylococcus aureus (ORSA) bloodstream isolates were tested to determine the prevalence of vancomycin heteroresistance. We screened 619 ORSA nosocomial bloodstream isolates from 36 hospitals between 1997 and 2000. Only one isolate exhibiting heterotypic resistance was detected. Thus, v...

journal_title：Journal of clinical microbiology

authors： Tallent SM,Bischoff T,Climo M,Ostrowsky B,Wenzel RP,Edmond MB

更新日期：2002-06-01 00:00:00

abstract：:Acute viral gastroenteritis is an intestinal infection that can be caused by several different viruses. Here we describe the evaluation and verification of Seeplex Diarrhea-V ACE (Seeplex DV), a novel commercial multiplex reverse transcription-PCR (RT-PCR) assay that detects 5 diarrheal pathogens, including adenovirus...

journal_title：Journal of clinical microbiology

authors： Higgins RR,Beniprashad M,Cardona M,Masney S,Low DE,Gubbay JB

更新日期：2011-09-01 00:00:00

abstract：:Hepatitis C virus (HCV) infection typically leads to antibody response within weeks after primary infection. Here, we describe the case of a child infected with HCV by mother-to-child transmission who remained persistently seronegative despite the presence of high levels of circulating HCV RNA. ...

journal_title：Journal of clinical microbiology

authors： Larouche A,Gaëtan G,El-Bilali N,Quesnel-Vallières M,Martin SR,Alvarez F,Shoukry NH,Soudeyns H

更新日期：2012-07-01 00:00:00

abstract：:Automated DNA sequencing was used to characterize mutations associated with rifampin resistance in a 69-bp region of the gene, rpoB, encoding the beta subunit of RNA polymerase in Mycobacterium tuberculosis. The data confirmed that greater than 90% of rifampin-resistant strains have sequence alterations in this region...

journal_title：Journal of clinical microbiology

authors： Kapur V,Li LL,Iordanescu S,Hamrick MR,Wanger A,Kreiswirth BN,Musser JM

更新日期：1994-04-01 00:00:00

abstract：:PCR-based molecular assays have a central role in polyomavirus diagnostics. To assure optimal performance, target sequences should be regularly updated according to newly available sequences. The aim of this study was to review our in-house polyomavirus BK (BKV) and JC (JCV) real-time PCR assays. Database analysis rev...

journal_title：Journal of clinical microbiology

authors： Dumoulin A,Hirsch HH

更新日期：2011-04-01 00:00:00

abstract：:To define the optimal conditions for human immunodeficiency virus (HIV) detection in microcultures, experiments were conducted with different ratios of patient and donor peripheral blood mononuclear cells (PBMCs). Donor/patient PBMC ratios ranged from 1:1 to 1:125. Optimal results were obtained when 1,500,000 donor ce...

journal_title：Journal of clinical microbiology

authors： Dimitrov DH,Melnick JL,Hollinger FB

更新日期：1990-04-01 00:00:00

abstract：:We describe the adoption of nucleic acid amplification tests (NAAT) for Clostridium difficile diagnosis and their impact on stool rejection policies and C. difficile positivity rates. Of the laboratories with complete surveys, 51 (43%) reported using NAAT in 2011. Laboratories using NAAT had stricter rejection policie...

journal_title：Journal of clinical microbiology

authors： Cohen J,Limbago B,Dumyati G,Holzbauer S,Johnston H,Perlmutter R,Dunn J,Nadle J,Lyons C,Phipps E,Beldavs Z,Clark LA,Lessa FC,CDC's Clostridium difficile Infection Surveillance Investigators.

更新日期：2014-02-01 00:00:00

abstract：:The development of diagnostic susceptibility tests for CEM-101, a new fluoroketolide, was addressed by structured studies to determine the optimal disk diffusion test concentration and effects of various testing conditions or supplements and to establish the quality control (QC) ranges for reference broth microdilutio...

journal_title：Journal of clinical microbiology

authors： Jones RN,Ross JE,Rhomberg PR,Quality Control Working Group.

更新日期：2010-04-01 00:00:00

abstract：:For a total of 29 non-human immunodeficiency virus 1 cryptococcal meningitis cases, titer changes in the latex agglutination test before and after therapy were reviewed along with clinical manifestations, laboratory findings, and therapy regimens. The cryptococcal antigen titer decreased for every case after therapy a...

journal_title：Journal of clinical microbiology

authors： Lu H,Zhou Y,Yin Y,Pan X,Weng X

更新日期：2005-06-01 00:00:00

abstract：:The BD Phoenix system was evaluated for species-level identification of yeasts (250 clinical isolates) and compared with the Vitek 2 system, using ribosomal internal transcribed spacer (ITS) sequence analysis as the gold standard. Considering only the species included in each system's database, 96.3% (236/245) and 91....

journal_title：Journal of clinical microbiology

authors： Posteraro B,Ruggeri A,De Carolis E,Torelli R,Vella A,De Maio F,Ricciardi W,Posteraro P,Sanguinetti M

更新日期：2013-11-01 00:00:00

abstract：:Clinically important species of Candida and related organisms were compared for extent of nucleotide divergence in the 5' end of the large-subunit (26S) ribosomal DNA (rDNA) gene. This rDNA region is sufficiently variable to allow reliable separation of all known clinically significant yeast species. Of the 204 descri...

journal_title：Journal of clinical microbiology

authors： Kurtzman CP,Robnett CJ

更新日期：1997-05-01 00:00:00

abstract：:The newly formulated Mycobacterium kansasii AccuProbe was evaluated, and the results obtained with the new version were compared to the results obtained with the old version of this test by using 116 M. kansasii strains, 1 Mycobacterium gastri strain, and 19 strains of several mycobacterial species. The sensitivity of...

journal_title：Journal of clinical microbiology

authors： Richter E,Niemann S,Rüsch-Gerdes S,Hoffner S

更新日期：1999-04-01 00:00:00

abstract：:Candida lipolytica was recovered from the blood and the central venous catheter in a patient receiving allogeneic bone marrow transplantation. Two C. lipolytica strains from different geographical areas and the ATCC 9773 strain of C. lipolytica were used as controls. C. lipolytica was identified by standard methods. M...

journal_title：Journal of clinical microbiology

authors： D'Antonio D,Romano F,Pontieri E,Fioritoni G,Caracciolo C,Bianchini S,Olioso P,Staniscia T,Sferra R,Boccia S,Vetuschi A,Federico G,Gaudio E,Carruba G

更新日期：2002-04-01 00:00:00

abstract：:Testing efforts for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been burdened by the scarcity of testing materials and personal protective equipment for health care workers. The simple and painless process of saliva collection allows for widespread testing, but enthusiasm is hampered by variable ...

journal_title：Journal of clinical microbiology

authors： Yee R,Truong TT,Pannaraj PS,Eubanks N,Gai E,Jumarang J,Turner L,Peralta A,Lee Y,Dien Bard J

更新日期：2021-01-21 00:00:00