Double-label fluorescence immunoassay of bacteria.

abstract：:The development of porcine epidemic diarrhea virus (PEDV) antibody-based assays is important for detecting infected animals, confirming previous virus exposure, and monitoring sow herd immunity. However, the potential cross-reactivity among porcine coronaviruses is a major concern for the development of pathogen-speci...

journal_title：Journal of clinical microbiology

authors： Gimenez-Lirola LG,Zhang J,Carrillo-Avila JA,Chen Q,Magtoto R,Poonsuk K,Baum DH,Piñeyro P,Zimmerman J

更新日期：2017-05-01 00:00:00

abstract：:An in-house enzyme-linked immunosorbent assay (ELISA) for measurement of Helicobacter pylori-specific immunoglobulin G (IgG) and IgA in saliva was evaluated by comparison with histopathologic (Giemsa staining) and biochemical (urease quick test) examination of gastric biopsy specimens obtained from 112 children referr...

journal_title：Journal of clinical microbiology

authors： Luzza F,Oderda G,Maletta M,Imeneo M,Mesuraca L,Chioboli E,Lerro P,Guandalini S,Pallone F

更新日期：1997-12-01 00:00:00

abstract：:Five serological assays were evaluated for the diagnosis of brucellosis in goats: the rose bengal test (RBT), complement fixation test (CFT), radial immunodiffusion (RID) with Brucella and Yersinia enterocolitica O:9 polysaccharides, counterimmunoelectrophoresis (CIEP) with cytosol, and enzyme-linked immunosorbent ass...

journal_title：Journal of clinical microbiology

authors： Díaz-Aparicio E,Marín C,Alonso-Urmeneta B,Aragón V,Pérez-Ortiz S,Pardo M,Blasco JM,Díaz R,Moriyón I

更新日期：1994-05-01 00:00:00

abstract：:Mycobacterium tuberculosis infection and nontuberculous mycobacteria (NTM) infections exhibit similar clinical symptoms; however, the therapies for these two types of infections are different. Therefore, the rapid and accurate identification of M. tuberculosis and NTM species is very important for the control of tuber...

journal_title：Journal of clinical microbiology

authors： Xiao G,He X,Zhang S,Liu Y,Liang Z,Liu H,Zhang J,Ou M,Cai S,Lai W,Zhang T,Ren L,Zhang G

更新日期：2020-01-28 00:00:00

abstract：:Production of staphylococcal alpha- or alpha-beta-toxins correlated well with production of coagulase or thermonuclease (or both) in 203 Staphylococcus aureus isolates from milk and should be reliable indicators of S. aureus in the absence of Staphylococcus intermedius. Failures to produce toxin, tube coagulase, or th...

journal_title：Journal of clinical microbiology

authors： Jasper DE,Infante F,Dellinger JD

更新日期：1985-04-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) for blastomycosis was evaluated with 65 human sera from culturally or histologically proven cases of blastomycosis, 53 sera from humans with heterologous infections, and 115 sera from apparently normal people. The diagnostic value of the ELISA was compared with that of the ...

journal_title：Journal of clinical microbiology

authors： Turner S,Kaufman L,Jalbert M

更新日期：1986-02-01 00:00:00

abstract：:The rate of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis (TB) has been steadily increasing in countries of the former USSR. The availability of rapid and reliable methods for the detection of drug resistance to second-line drugs is vital for adequate patient management. We evaluated the ...

journal_title：Journal of clinical microbiology

authors： Ignatyeva O,Kontsevaya I,Kovalyov A,Balabanova Y,Nikolayevskyy V,Toit K,Dragan A,Maxim D,Mironova S,Kummik T,Muntean I,Koshkarova E,Drobniewski F

更新日期：2012-05-01 00:00:00

abstract：:Rabies virus found worldwide and prevalent throughout the United States continues to be a public health concern. Direct-fluorescent antibody (DFA) detection remains the gold standard for rabies virus diagnostics. Assessing the utility of a high-throughput molecular platform such as the QIAsymphony SP/AS, in conjunctio...

journal_title：Journal of clinical microbiology

authors： Dupuis M,Brunt S,Appler K,Davis A,Rudd R

更新日期：2015-09-01 00:00:00

abstract：:Early diagnosis of Neisseria gonorrhoeae infections is important with regard to patients' health and infectivity. We report the development of a specific and sensitive TaqMan assay for the detection of N. gonorrhoeae in clinical samples. The target sequence is a 76-bp fragment of the 5' untranslated region of the opa ...

journal_title：Journal of clinical microbiology

authors： Geraats-Peters CW,Brouwers M,Schneeberger PM,van der Zanden AG,Bruisten SM,Weers-Pothoff G,Boel CH,van den Brule AJ,Harmsen HG,Hermans MH

更新日期：2005-11-01 00:00:00

abstract：:We studied whether the Etest can be used as an alternative to agar dilution to determine antimicrobial susceptibilities of ceftriaxone, cefixime, and cefpodoxime in Neisseria gonorrhoeae surveillance. One hundred fifteen clinical and laboratory isolates of N. gonorrhoeae were tested following the Clinical Laboratory I...

journal_title：Journal of clinical microbiology

authors： Liu H,Taylor TH Jr,Pettus K,Trees D

更新日期：2014-05-01 00:00:00

abstract：:This study assessed the diagnostic performance of the new COVID19SEROSpeed IgM/IgG rapid test (BioSpeedia, a spinoff of the Pasteur Institute of Paris) for the detection of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in comparison to other commercial antibody assays through a large ...

journal_title：Journal of clinical microbiology

authors： Plebani M,Parčina M,Bechri I,Zehender G,Terkeš V,Abdel Hafith B,Antinori S,Pillet S,Gonzalo S,Hoerauf A,Lai A,Morović M,Bourlet T,Torre A,Pozzetto B,Galli M

更新日期：2021-01-21 00:00:00

abstract：:Quantitative real-time PCR for the detection of respiratory syncytial virus (RSV) RNA is increasingly used to study the causal role of RSV in lower airway disease. The objective of our study was to evaluate variations in RSV RNA loads at different steps in the RNA quantification process: (i) variation in RSV RNA load ...

journal_title：Journal of clinical microbiology

authors： van de Pol AC,Wolfs TF,van Loon AM,Tacke CE,Viveen MC,Jansen NJ,Kimpen JL,Rossen JW,Coenjaerts FE

更新日期：2010-10-01 00:00:00

abstract：:Yarrowia lipolytica is a weakly pathogenic yeast that is rarely isolated from the blood. We observed transient recurrent catheter-related fungemia attributable to this organism in a leukemic patient. The fungemia and accompanying fever subsided spontaneously. The data suggest that it might be possible to withhold spec...

journal_title：Journal of clinical microbiology

authors： Chang CL,Park TH,Lee EY,Lim YT,Son HC

更新日期：2001-03-01 00:00:00

abstract：:A total of 28 unrelated isolates of the Salmonella enterica subsp. enterica serovar dublin (S. dublin) collected during a 6-year period, as well as four samples of the S. dublin live vaccine strain Bovisaloral and its prototype strain S. dublin 442/039, were investigated by different molecular typing methods for the f...

journal_title：Journal of clinical microbiology

authors： Liebisch B,Schwarz S

更新日期：1996-03-01 00:00:00

abstract：:From August 1996 to May 1997, six verotoxin-producing Escherichia coli (VTEC) strains were isolated from stool specimens of adults suffering from hemolytic-uremic syndrome (HUS). All the isolates were stx2 positive and belonged to different serotypes: O6:H4, O91:H10, O91:H21, O rough:H16, OX3:H-, and O nontypeable: H-...

journal_title：Journal of clinical microbiology

authors： Bonnet R,Souweine B,Gauthier G,Rich C,Livrelli V,Sirot J,Joly B,Forestier C

更新日期：1998-06-01 00:00:00

abstract：:A direct comparison of two automated blood culture systems was conducted to compare their ability to detect Candida growth. The systems evaluated were the BACTEC 9240 (Bactec) and BacT/ALERT 3D (BacT). The aerobic, anaerobic, and mycology media for each system were evaluated: Bactec Plus Aerobic/F, Plus Anaerobic/F, a...

journal_title：Journal of clinical microbiology

authors： Horvath LL,George BJ,Murray CK,Harrison LS,Hospenthal DR

更新日期：2004-01-01 00:00:00

abstract：:When the standard procedure for determining antibiotic susceptibility of bacteria is used, the results are delayed, especially for bacteria that grow slowly, such as Helicobacter pylori. Treatment for this bacterium may involve clarithromycin, a compound for which resistance has been associated with point mutations on...

journal_title：Journal of clinical microbiology

authors： Pina M,Occhialini A,Monteiro L,Doermann HP,Mégraud F

更新日期：1998-11-01 00:00:00

abstract：:In a survey of bloodstream infection (BSI) isolates across the continental United States, 162 Candida albicans isolates were fingerprinted with the species-specific probe Ca3 and the patterns were analyzed for relatedness with a computer-assisted system. The results demonstrate that particular BSI strains are more hig...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Lockhart SR,Pujol C,Swails-Wenger JA,Messer SA,Edmond MB,Jones RN,Wenzel RP,Soll DR

更新日期：1998-06-01 00:00:00

abstract：:A multiplexed biomarker bundle consisting of nine different inflammation markers was evaluated regarding their diagnostic and prognostic performances in 159 adult systemic inflammatory response syndrome (SIRS) patients enrolled at the emergency department. Fibronectin, interleukin-8 (IL-8), biotin, and neutrophil gela...

journal_title：Journal of clinical microbiology

authors： Reichsoellner M,Raggam RB,Wagner J,Krause R,Hoenigl M

更新日期：2014-11-01 00:00:00

abstract：:Serratia rubidaea biotype 1 was isolated from the bile and blood of a patient with a bile tract carcinoma obstructing the common bile duct and who underwent invasive procedures. The infection was cleared after adequate treatment with antibiotics. ...

journal_title：Journal of clinical microbiology

authors： Ursua PR,Unzaga MJ,Melero P,Iturburu I,Ezpeleta C,Cisterna R

更新日期：1996-01-01 00:00:00

abstract：:Thirteen representative pathogenic bacterial species were used to create septicemia in rabbits, by injecting 10(6) colony-forming units into the marginal ear vein. At a selected time, usually 30 to 60 min after injection, heart blood was drawn into heparin and dispensed in 5.0-,0.5-, and 0.1-ml volumes into duplicate ...

journal_title：Journal of clinical microbiology

authors： Zierdt CH,Peterson DL,Swan JC,MacLowry JD

更新日期：1982-01-01 00:00:00

abstract：:Minimum essential medium supplemented with either 7% fetal bovine serum or 2% fetal bovine serum and 1.5% low protein serum replacement (Sigma Chemical Co., St. Louis, Mo.) factor was used to evaluate the growth of Vero 76 cells (E-6 clone) in a cell culture bioreactor. Three different microcarriers, collagen-coated p...

journal_title：Journal of clinical microbiology

authors： White LA,Ades EW

更新日期：1990-02-01 00:00:00

abstract：:The comparative susceptibilities for rhinovirus isolation were determined for strain MRC-5 human embryonic lung fibroblast and human fetal tonsil cells. Samples from nasopharyngeal and throat swab clinical specimens were inoculated onto duplicate monolayers of each cell type. Of 105 rhinovirus-positive specimens, 78 (...

journal_title：Journal of clinical microbiology

authors： Geist FC,Hayden FG

更新日期：1985-09-01 00:00:00

abstract：:A double-antibody (sandwich) enzyme-linked immunosorbent assay (ELISA) was adapted to detect Rift Valley fever virus antigen. Antibodies were purified from hyperimmune mouse and rabbit sera by affinity chromatography, using CNBr-activated Sepharose 4B coupled to a beta-propiolactone-inactivated sucrose-acetone-extract...

journal_title：Journal of clinical microbiology

authors： Niklasson B,Grandien M,Peters CJ,Gargan TP 2nd

更新日期：1983-06-01 00:00:00

abstract：:The usefulness of pneumococcal urinary antigen tests (UATs) in severe pneumococcal infection relies heavily on the performance in bacteremic patients. Fluorescence technology and automatic reading of test results may improve UAT performance. We evaluated the automatically read Sofia S. pneumoniae FIA for diagnosing pn...

journal_title：Journal of clinical microbiology

authors： Olofsson E,Özenci V,Athlin S

更新日期：2019-07-26 00:00:00

abstract：:Members of the genus Malassezia, lipophilic yeasts, are considered to be one of the exacerbating factors in atopic dermatitis (AD). We examined variation in cutaneous colonization by Malassezia species in AD patients and compared it with variation in healthy subjects. Samples were collected by applying transparent dre...

journal_title：Journal of clinical microbiology

authors： Sugita T,Suto H,Unno T,Tsuboi R,Ogawa H,Shinoda T,Nishikawa A

更新日期：2001-10-01 00:00:00

abstract：:Influenza viruses related to the markedly antigenically divergent strains B/Yamagata/16/88 and B/Victoria/2/87 are circulating in human populations. Adults develop cross-reacting antibodies against recent and earlier influenza B virus strains after vaccination with B/Yamagata/16/88, probably because of previous influe...

journal_title：Journal of clinical microbiology

authors： Levandowski RA,Gross PA,Weksler M,Staton E,Williams MS,Bonelli J

更新日期：1991-07-01 00:00:00

abstract：:A PCR method for the unequivocal assignment of Haemophilus influenzae capsular type (types a to f) was developed. PCR primers were designed from capsule type-specific DNA sequences cloned from the capsular gene cluster of each of the six capsular types. PCR product was amplified only from the capsular type for which t...

journal_title：Journal of clinical microbiology

authors： Falla TJ,Crook DW,Brophy LN,Maskell D,Kroll JS,Moxon ER

更新日期：1994-10-01 00:00:00

abstract：:Antibiotic-associated hemorrhagic colitis (AAHC) is associated with Klebsiella oxytoca. This study analyzed whether cytotoxic properties are linked to specific subtypes of K. oxytoca. Klebsiella isolates from stools of AAHC patients, healthy carriers, and diarrhea patients as well as from infections of other organs we...

journal_title：Journal of clinical microbiology

authors： Joainig MM,Gorkiewicz G,Leitner E,Weberhofer P,Zollner-Schwetz I,Lippe I,Feierl G,Krause R,Hinterleitner T,Zechner EL,Högenauer C

更新日期：2010-03-01 00:00:00

abstract：:We attempted to find a relationship between the microbiological properties of bloodstream isolates of methicillin-resistant Staphylococcus aureus (MRSA) and the efficacy of vancomycin in the treatment of bacteremia. Vancomycin susceptibility testing was performed, and bactericidal activity was determined for 30 isolat...

journal_title：Journal of clinical microbiology

authors： Sakoulas G,Moise-Broder PA,Schentag J,Forrest A,Moellering RC Jr,Eliopoulos GM

更新日期：2004-06-01 00:00:00