Characterization by automated DNA sequencing of mutations in the gene (rpoB) encoding the RNA polymerase beta subunit in rifampin-resistant Mycobacterium tuberculosis strains from New York City and Texas.

abstract：:Pyrazinamide is important in tuberculosis treatment, as it is bactericidal to semidormant mycobacteria not killed by other antituberculosis drugs. Pyrazinamide is also one of the cornerstone drugs retained in the treatment of multidrug-resistant tuberculosis (MDR-TB). However, due to technical difficulties, routine dr...

journal_title：Journal of clinical microbiology

authors： Mphahlele M,Syre H,Valvatne H,Stavrum R,Mannsåker T,Muthivhi T,Weyer K,Fourie PB,Grewal HM

更新日期：2008-10-01 00:00:00

abstract：:We recently tested serum from a human immunodeficiency virus-infected patient for the presence of cryptococcal antigen using the Meridian latex agglutination (LA) test (Cryptococcal Antigen Latex Agglutination System). Two pronase-treated serum specimens from the patient had LA titers of 80 and 160, but the patient ha...

journal_title：Journal of clinical microbiology

authors： Whittier S,Hopfer RL,Gilligan P

更新日期：1994-09-01 00:00:00

abstract：:A ligation-mediated PCR (LMPCR) method for the amplification of sequences flanking the IS6110 of the Mycobacterium tuberculosis complex has been developed. The method uses one primer specific for IS6110 and a second specific for a linker ligated to SalI-restricted genomic DNA. LMPCR is a rapid screening method, valuab...

journal_title：Journal of clinical microbiology

authors： Prod'hom G,Guilhot C,Gutierrez MC,Varnerot A,Gicquel B,Vincent V

更新日期：1997-12-01 00:00:00

abstract：:Three hundred sixty Enterobacteriaceae and nonfermenting gram-negative bacilli, isolated during one week in May 2004 at five hospitals in Amsterdam, The Netherlands, were evaluated for the presence of extended-spectrum beta-lactamases (ESBLs). A prevalence of 7.8% was found, in contrast to the 1% observed in 1997. CTX...

journal_title：Journal of clinical microbiology

authors： al Naiemi N,Bart A,de Jong MD,Vandenbroucke-Grauls CM,Rietra PJ,Debets-Ossenkopp YJ,Wever PC,Spanjaard L,Bos AJ,Duim B

更新日期：2006-08-01 00:00:00

abstract：:A microagglutination test for Francisella tularensis, with 0.025-ml amounts of diluted sera and 0.025-ml amounts of safranin-O-stained antigen in U-bottom microtitration plates, was compared with a tube agglutination test by using 137 sera. There was 86.3% agreement (+/- 1 dilution variation) between the microagglutin...

journal_title：Journal of clinical microbiology

authors： Brown SL,McKinney FT,Klein GC,Jones WL

更新日期：1980-02-01 00:00:00

abstract：:Total DNA from five Campylobacter species was digested with a mixture of XhoI and BglII restriction endonucleases and analyzed by Southern hybridization by using a probe complementary to the DNA coding for the 16S rRNA. Each of the Campylobacter species, including C. jejuni, C. coli, C. laridis, C. fetus, and C. upsal...

journal_title：Journal of clinical microbiology

authors： Moureau P,Derclaye I,Gregoire D,Janssen M,Cornelis GR

更新日期：1989-07-01 00:00:00

abstract：:In this study, we determined the sequences of four intimin variant genes detected in attaching and effacing Escherichia coli isolates of human origin. Three of them were novel and were designated eae-eta (eta), eae-iota (iota), and eae-kappa (kappa). The fourth was identical to the recently described eae-zeta (zeta), ...

journal_title：Journal of clinical microbiology

authors： Zhang WL,Köhler B,Oswald E,Beutin L,Karch H,Morabito S,Caprioli A,Suerbaum S,Schmidt H

更新日期：2002-12-01 00:00:00

abstract：:Serogrouping of Bacteroides nodosus is based on antigenic differences in fimbriae of the different New Zealand prototype strains. Because of the time needed to isolate and grow pure cultures of B. nodosus and the difficulty in distinguishing between different serogroups because of cross-agglutination, a new DNA-based ...

journal_title：Journal of clinical microbiology

authors： John GH,Carlson JO,Kimberling CV,Ellis RP

更新日期：1990-11-01 00:00:00

abstract：:Simple enzymatic assays to detect heat-labile enterotoxins whose modes of action are similar to that of cholera toxin were evaluated. The assays are performed by using an artificial substrate, diethylamino benzylidine-aminoguanidine, which is an ADP-ribose acceptor. The product, formed in the presence of NAD+, can be ...

journal_title：Journal of clinical microbiology

authors： Narayanan J,Hartman PA,Graves DJ

更新日期：1989-11-01 00:00:00

abstract：:Campylobacter jejuni (20 strains) and Campylobacter coli (12 strains) were assigned to four biovars for each species based on phenotypic tests that were easy to perform and interpret. The resulting biotyping schemes offer a greater degree of distinction among C. jejuni and C. coli strains than any of the other biotypi...

journal_title：Journal of clinical microbiology

authors： Roop RM 2nd,Smibert RM,Krieg NR

更新日期：1984-11-01 00:00:00

abstract：:A study was conducted to compare the Bac-T-Screen Bacterial Detection Device for Urines (BDD; Marion Laboratories, Kansas City, Mo.) with urine Gram stain as a screen for bacteriuria. We analyzed 631 urine samples with the BDD and compared the results to urine Gram stains and quantitative cultures. A total of 90 (14%)...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Baum CA,Niles AC,Murray PR

更新日期：1983-09-01 00:00:00

abstract：:Many laboratory workers prefer the rhodamine-auramine method of staining acid-fast bacilli (AFB) in primary specimen smears rather than carbol fuchsin stains because the stain is more readily interpreted and yields greater sensitivity. The increasing incidence of AFB infections serves as an impetus to optimize the rho...

journal_title：Journal of clinical microbiology

authors： McCarter YS,Robinson A

更新日期：1994-10-01 00:00:00

abstract：:Although screening for maternal toxoplasmic seroconversion during pregnancy is based on immunodiagnostic assays, the diagnosis of clinically relevant toxoplasmosis greatly relies upon molecular methods. A problem is that this molecular diagnosis is subject to variation of performances, mainly due to a large diversity ...

journal_title：Journal of clinical microbiology

authors： Sterkers Y,Varlet-Marie E,Cassaing S,Brenier-Pinchart MP,Brun S,Dalle F,Delhaes L,Filisetti D,Pelloux H,Yera H,Bastien P

更新日期：2010-09-01 00:00:00

abstract：:The main goal of this study was to determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA), particularly livestock-associated MRSA (LA-MRSA) in pigs and pork. The genotypic relatedness of isolates on the farm, at slaughter, and at the retail level was assessed. Paired nasal and perianal swab sa...

journal_title：Journal of clinical microbiology

authors： Molla B,Byrne M,Abley M,Mathews J,Jackson CR,Fedorka-Cray P,Sreevatsan S,Wang P,Gebreyes WA

更新日期：2012-11-01 00:00:00

abstract：:High-dose cefepime-tazobactam (1:1; WCK 4282), a novel antibacterial combination consisting of the β-lactamase inhibitor tazobactam and a fourth-generation cephalosporin, is under clinical development for the treatment of serious Gram-negative infections. A quality control (QC) study was performed to establish disk di...

journal_title：Journal of clinical microbiology

authors： Riedel S,Huband MD,Sader HS,Flamm RK,Jones RN

更新日期：2017-10-01 00:00:00

abstract：:In 32 patients for whom highly active antiretroviral therapy was failing, a good agreement between drug resistance-associated mutations in plasma and peripheral blood mononuclear cells (PBMCs) was found (k = 0.85). The mutations with the lowest agreement were 20R, 63P, and 84V in the protease gene and 184V in the reve...

journal_title：Journal of clinical microbiology

authors： Sarmati L,Nicastri E,Uccella I,D'Ettorre G,Parisi SG,Palmisano L,Galluzzo C,Concia E,Vullo V,Vella S,Andreoni M

更新日期：2003-04-01 00:00:00

abstract：:A clinical evaluation was conducted in six North American centers to determine the ability of APTIMA CT (ACT) and APTIMA GC (AGC) nucleic acid amplification assays to detect Chlamydia trachomatis and Neisseria gonorrhoeae infections in 1,322 men by testing their urethral swabs and first-catch urine (FCU). The results ...

journal_title：Journal of clinical microbiology

authors： Chernesky MA,Martin DH,Hook EW,Willis D,Jordan J,Wang S,Lane JR,Fuller D,Schachter J

更新日期：2005-01-01 00:00:00

abstract：:The API Staph-Ident system was evaluated as a means for identifying the species of bovine strains of staphylococci routinely isolated from quarter-milk samples. The species identity of 314 of 581 (54%) isolates of staphylococci was correctly determined by this method. The API Staph-Ident system was more accurate in co...

journal_title：Journal of clinical microbiology

authors： Langlois BE,Harmon RJ,Akers K

更新日期：1983-11-01 00:00:00

abstract：:A reverse transcriptase PCR (RT-PCR) was used for rapid determination of the hemagglutinin (HA) cleavage site sequence, a marker for the virulence potential of avian influenza viruses. When applied to specimens from chickens experimentally infected with either a virulent or an avirulent virus, RT-PCR uniformly detecte...

journal_title：Journal of clinical microbiology

authors： Horimoto T,Kawaoka Y

更新日期：1995-03-01 00:00:00

abstract：:A lymph node excision was performed on a 45-year-old woman with left cervical swelling. The disorder which developed after the patient had undergone oral surgery for a severe periodontal disease failed to respond to antimicrobial chemotherapy. A mycobacterial strain subsequently identified by high-performance liquid c...

journal_title：Journal of clinical microbiology

authors： Piersimoni C,Goteri G,Nista D,Mariottini A,Mazzarelli G,Bornigia S

更新日期：2004-08-01 00:00:00

abstract：:We studied 315 coagulase-negative Staphylococcus strains recovered prospectively during 240 surgical procedures (206 subjects) from proven or suspected device-associated bone and joint infections. Sixteen strains (5.1%) had decreased susceptibility to glycopeptides: 15 (12 S. epidermidis strains, 2 S. capitis strains,...

journal_title：Journal of clinical microbiology

authors： Cremniter J,Slassi A,Quincampoix JC,Sivadon-Tardy V,Bauer T,Porcher R,Lortat-Jacob A,Piriou P,Judet T,Herrmann JL,Gaillard JL,Rottman M

更新日期：2010-04-01 00:00:00

abstract：:Cryptococcus gattii emerged in 1999 in a distinct ecologic environment on Vancouver Island, Canada. Estimates of incubation period were derived from an analysis of travelers with discreet exposures to this region. Individual case incubation periods ranged from 2 to 11 months, with a median of 6 to 7 months. ...

journal_title：Journal of clinical microbiology

authors： MacDougall L,Fyfe M

更新日期：2006-05-01 00:00:00

abstract：:Eleven isolates representing five distinct outbreaks of pneumococcal conjunctivitis were examined for phenotypic and genetic characteristics. None of the strains possessed capsules, and all strains were susceptible to optochin, bile soluble, and Gen-Probe AccuProbe test positive. All 11 isolates were confirmed as Stre...

journal_title：Journal of clinical microbiology

authors： Carvalho MG,Steigerwalt AG,Thompson T,Jackson D,Facklam RR

更新日期：2003-09-01 00:00:00

abstract：:The low incidence of group B streptococcal (GBS) invasive neonatal disease in Mexico has been attributed to the low prevalence of serotype III strains, a major serotype in developed countries. In addition, nontypeable strains account for 12% of the isolates in Mexico and < 1% of the isolates in the United States. In t...

journal_title：Journal of clinical microbiology

authors： Palacios GC,Eskew EK,Solorzano F,Mattingly SJ

更新日期：1997-11-01 00:00:00

abstract：:Bordetella pertussis was transmitted from an immunized boy to his father and possibly to other family members. This case report demonstrates that although unusual, acute adult B. pertussis infection can occur. B. pertussis immunization may not prevent infection but can reduce the severity. B. pertussis was detected in...

journal_title：Journal of clinical microbiology

authors： Smith S,Tilton RC

更新日期：1996-02-01 00:00:00

abstract：:Salmonella enterica serovar Heidelberg is the second most frequently occurring serovar in Quebec and the third-most prevalent in Canada. Given that conventional pulsed-field gel electrophoresis (PFGE) subtyping for common Salmonella serovars, such as S. Heidelberg, yields identical subtypes for the majority of isolate...

journal_title：Journal of clinical microbiology

authors： Bekal S,Berry C,Reimer AR,Van Domselaar G,Beaudry G,Fournier E,Doualla-Bell F,Levac E,Gaulin C,Ramsay D,Huot C,Walker M,Sieffert C,Tremblay C

更新日期：2016-02-01 00:00:00

abstract：:Evolution of the direct repeat region in Mycobacterium tuberculosis has created unique spoligotype signatures specifically associated with IS6110-defined strain families. Spoligotyping signatures may enable the analysis of the strain population structure in different settings and will enable the rapid identification o...

journal_title：Journal of clinical microbiology

authors： Streicher EM,Victor TC,van der Spuy G,Sola C,Rastogi N,van Helden PD,Warren RM

更新日期：2007-01-01 00:00:00

abstract：:The hemagglutination inhibition test (HAI) and the enzyme-linked immunosorbent assay (ELISA) for detecting antibody to rubella virus were compared by testing 25 sets of paired sera taken before and after infection and 10 sets of sera taken during acute and convalescent stages of the disease and by screening 700 serum ...

journal_title：Journal of clinical microbiology

authors： Shekarchi IC,Sever JL,Tzan N,Ley A,Ward LC,Madden D

更新日期：1981-05-01 00:00:00

abstract：:Blood culture records from 1994 to 1995 from five U.S. medical centers all using the Difco ESP continuous monitoring blood culture system were reviewed retrospectively. Among a total of 7,362 isolates of bacteria and yeasts, only 0.1% of possibly significant isolates would have been missed had blood cultures been rout...

journal_title：Journal of clinical microbiology

authors： Doern GV,Brueggemann AB,Dunne WM,Jenkins SG,Halstead DC,McLaughlin JC

更新日期：1997-05-01 00:00:00

abstract：:Porphyromonas gingivalis is one of the major causative agents of adult periodontitis. One of the features of this periodontal pathogen is its ability to attach to a variety of oral bacterial surfaces and to colonize subgingival dental plaque. We have shown that Streptococcus cristatus CC5A inhibits expression of fimA,...

journal_title：Journal of clinical microbiology

authors： Wang BY,Wu J,Lamont RJ,Lin X,Xie H

更新日期：2009-12-01 00:00:00