Identification of Mycobacterium kansasii by using a DNA probe (AccuProbe) and molecular techniques.

abstract：:A new direct immunofluorescence monoclonal antibody (DFA) method (Genetic Systems, Inc., Seattle, Wash.) for identification of Pneumocystis carinii in induced sputum and bronchoalveolar lavage specimens was compared in a blinded study with an established Giemsa stain method. We evaluated 148 consecutive clinical speci...

journal_title：Journal of clinical microbiology

authors： Wolfson JS,Waldron MA,Sierra LS

更新日期：1990-09-01 00:00:00

abstract：:From November 2000 to October 2001, a reverse transcription-PCR using primers directed to the norovirus RNA polymerase coding region was included in a viral and bacterial routine screening to diagnose sporadic cases of acute gastroenteritis among children in Asturias, Spain. The role of noroviruses (8.6% of the positi...

journal_title：Journal of clinical microbiology

authors： Boga JA,Melón S,Nicieza I,De Diego I,Villar M,Parra F,De Oña M

更新日期：2004-06-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay for the measurement of antibodies directed against cell surface antigens of Vibrio cholerae (CSA ELISA) was developed. NaN3-killed whole cells of V. cholerae, adsorbed to polystyrene tubes, were used as immobilized antigens. The assay was capable of detecting antibodies directed ag...

journal_title：Journal of clinical microbiology

authors： Cryz SJ Jr,Fürer E,Germanier R

更新日期：1982-07-01 00:00:00

abstract：:A colony blot enzyme-linked immunosorbent assay was developed for the rapid identification of smooth Brucella species, i.e., Brucella abortus, B. melitensis, and B. suis. Bacterial colonies from plates were blotted onto nitrocellulose disks, lysed by immersion in chloroform, and reacted with BRU 38, a rat monoclonal a...

journal_title：Journal of clinical microbiology

authors： Roop RM 2nd,Preston-Moore D,Bagchi T,Schurig GG

更新日期：1987-11-01 00:00:00

abstract：:The performance of four media for the isolation of Aeromonas strains from stool specimens, the importance of ampicillin-susceptible Aeromonas strains in the selection of culture media, and the usefulness of beta-hemolysis in screening blood-containing media for Aeromonas strains were evaluated in two phases. In the fi...

journal_title：Journal of clinical microbiology

authors： Kelly MT,Stroh EM,Jessop J

更新日期：1988-09-01 00:00:00

abstract：:Burkholderia pseudomallei is the causative agent of melioidosis. This condition most often presents as pneumonia and bacteremia, with mortality rates of 9% to 70%. Therefore, early identification of this organism may aid in directing appropriate management. This study aimed to use the Vitek matrix-assisted laser desor...

journal_title：Journal of clinical microbiology

authors： Gassiep I,Armstrong M,Norton RE

更新日期：2019-04-26 00:00:00

abstract：:Chronic hepatitis B virus (HBV) infection via perinatal transmission is common in the Asia-Pacific region, but related quasispecies (QS) characteristics are not yet defined. To investigate the homologous, full-length HBV QS after perinatal infection and their clinical implications, five pairs of mother-daughter patien...

journal_title：Journal of clinical microbiology

authors： Liu F,Yu DM,Huang SY,Yu JL,Zhang DH,Gong QM,Zhang XX

更新日期：2014-05-01 00:00:00

abstract：:Pathozyme-Myco G (Myco G), M, A, and TB complex plus (Omega Diagnostics Ltd., Alloa, Scotland) were evaluated for the serological diagnosis of pulmonary tuberculosis (TB) in an Argentinean population. Sera from 58 patients with pulmonary TB, 24 subjects with pulmonary mycobacteriosis or mycoses (pulmonary MM group), a...

journal_title：Journal of clinical microbiology

authors： Imaz MS,Comini MA,Zerbini E,Sequeira MD,Latini O,Claus JD,Singh M

更新日期：2004-02-01 00:00:00

abstract：:Streptococcus pyogenes, a major human pathogen, is still considered susceptible to beta-lactams, but for other relevant antibiotics, highly variable resistance rates have been reported. Since no data were available from Iran, we tested 1,335 throat isolates from two different regions of the country for their antibioti...

journal_title：Journal of clinical microbiology

authors： Jasir A,Tanna A,Noorani A,Mirsalehian A,Efstratiou A,Schalen C

更新日期：2000-06-01 00:00:00

abstract：:In the United Kingdom there has been a marked increase in the number of human infections caused by toxigenic Corynebacterium ulcerans. During 2002 and 2003 the organism was also isolated from several domestic cats with bilateral nasal discharge. As C. ulcerans has never previously been isolated from cats, the 16S rRNA...

journal_title：Journal of clinical microbiology

authors： De Zoysa A,Hawkey PM,Engler K,George R,Mann G,Reilly W,Taylor D,Efstratiou A

更新日期：2005-09-01 00:00:00

abstract：:Hepatitis B surface antigen was concentrated and purified from plasma by two simple steps of purification. In the first step the antigen was purified 24-fold by polyethylene glycol precipitation. An additional 10-fold purification was achieved by batchwise adsorption to hydroxylapatite and subsequent elution with 0.02...

journal_title：Journal of clinical microbiology

authors： Vnek J,Prince AM

更新日期：1976-06-01 00:00:00

abstract：:Examination of a number of previously published aligned Borrelia 16S rRNA sequences revealed the presence of regions which could serve as oligonucleotide probe targets for both species-specific identification of Borrelia burgdorferi and distinction between genomic groups. Total cellular RNA isolated from Borrelia cult...

journal_title：Journal of clinical microbiology

authors： Marconi RT,Lubke L,Hauglum W,Garon CF

更新日期：1992-03-01 00:00:00

abstract：:A procedure was developed for characterization of Chlamydia trachomatis strains by using restriction endonuclease analysis of amplified genes of the major outer membrane protein (MOMP). Reference strains of the 15 serovars (A through K and L1 through L3) and clinical isolates were tested. The nucleotide sequences of t...

journal_title：Journal of clinical microbiology

authors： Rodriguez P,Vekris A,de Barbeyrac B,Dutilh B,Bonnet J,Bebear C

更新日期：1991-06-01 00:00:00

abstract：:Counterimmunoelectrophoresis was compared with electron microscopy for detection of human reovirus-like agent in fecal specimens. Both tests gave very similar results. ...

journal_title：Journal of clinical microbiology

authors： Spence L,Fauvel M,Petro R,Bloch S

更新日期：1977-02-01 00:00:00

abstract：:Prototheca spp. are environmental algae that may cause serious infection in the immunocompromised patient. Clinical manifestations may mimic other diseases. We present a case of fatal infection in a 78-year-old cardiac transplant recipient and discuss pitfalls in the clinical and laboratory diagnoses. ...

journal_title：Journal of clinical microbiology

authors： McMullan B,Muthiah K,Stark D,Lee L,Marriott D

更新日期：2011-08-01 00:00:00

abstract：:A modified oxidase reagent, 1% tetramethyl-p-phenylenediamine in dimethyl sulfoxide, proved superior to the routinely used 1% aqueous tetramethyl-p-phenylenediamine dihydrochloride in detecting weakly oxidase-positive gram-negative bacteria after 24 h of growth on agar media (40 of 40 positive versus 22 of 40 positive...

journal_title：Journal of clinical microbiology

authors： Tarrand JJ,Gröschel DH

更新日期：1982-10-01 00:00:00

abstract：:PCR amplification technology for the detection of epizootic hemorrhagic disease virus (EHDV) ribonucleic acid in cell culture and clinical specimens was developed. With oligoribonucleotide primers selected from genome segment 10 of EHDV serotype 1 (EHDV-1), which codes for two nonstructural proteins (NS3 and NS3a), th...

journal_title：Journal of clinical microbiology

authors： Aradaib IE,Wilson WC,Schore CE,Mohammed ME,Yilma TD,Cullor JS,Osburn BI

更新日期：1998-09-01 00:00:00

abstract：:Since influenza viruses can cause severe illness, timely diagnosis is important for an adequate intervention. The available rapid detection methods either lack sensitivity or require complex laboratory manipulation. This study describes a rapid, sensitive detection method that can be easily applied to routine diagnosi...

journal_title：Journal of clinical microbiology

authors： van Elden LJ,Nijhuis M,Schipper P,Schuurman R,van Loon AM

更新日期：2001-01-01 00:00:00

abstract：:Recent reports of natural paratuberculosis (or Johne's disease) in rabbits, foxes, and stoats has focused debate on the presence and importance of wildlife reservoirs in the epidemiology of this disease. This paper describes an extensive study investigating 18 nonruminant wildlife species for evidence of paratuberculo...

journal_title：Journal of clinical microbiology

authors： Beard PM,Daniels MJ,Henderson D,Pirie A,Rudge K,Buxton D,Rhind S,Greig A,Hutchings MR,McKendrick I,Stevenson K,Sharp JM

更新日期：2001-04-01 00:00:00

abstract：:Data are limited for the performance of enzyme immunoassays for the detection of Chlamydia trachomatis in conjunctival and nasopharyngeal specimens from infants. The only available data are for one assay, Chlamydiazyme (Abbott Diagnostics). The purpose of this study was to compare a new enzyme immunoassay, Pathfinder ...

journal_title：Journal of clinical microbiology

authors： Hammerschlag MR,Roblin PM,Gelling M,Worku M

更新日期：1990-08-01 00:00:00

abstract：:Although vaginitis caused by Saccharomyces cerevisiae is extremely rare, in recent years we have experienced an increasing frequency of S. cerevisiae isolation from the vaginas of fertile-age women. In order to investigate the epidemiology of these vaginal infections, a total of 40 isolates of S. cerevisiae derived fr...

journal_title：Journal of clinical microbiology

authors： Posteraro B,Sanguinetti M,D'Amore G,Masucci L,Morace G,Fadda G

更新日期：1999-07-01 00:00:00

abstract：:The mainstay of laboratory diagnosis for Lyme disease is two-tiered serological testing, in which a reactive first-tier enzyme-linked immunosorbent assay (ELISA) or an immunofluorescence assay is supplemented by separate IgM and IgG immunoblots. Recent data suggest that the C6 ELISA can be substituted for immunoblots ...

journal_title：Journal of clinical microbiology

authors： Wormser GP,Levin A,Soman S,Adenikinju O,Longo MV,Branda JA

更新日期：2013-12-01 00:00:00

abstract：:We compared a new colony hybridization assay with an established enzyme-linked immunosorbent assay for detection of enterotoxigenic Escherichia coli (ETEC) expressing colonization factor antigen I (CFA/I). The tests were applied to 135 human ETEC strains. Of these isolates, 30 had previously been characterized for CFA...

journal_title：Journal of clinical microbiology

authors： Grewal HM,Sommerfelt H,Gaastra W,Svennerholm AM,Bhan MK,Hamers AM,Kumar R,Wiklund G,Bjorvatn B

更新日期：1990-10-01 00:00:00

abstract：:A microagglutination test for Francisella tularensis, with 0.025-ml amounts of diluted sera and 0.025-ml amounts of safranin-O-stained antigen in U-bottom microtitration plates, was compared with a tube agglutination test by using 137 sera. There was 86.3% agreement (+/- 1 dilution variation) between the microagglutin...

journal_title：Journal of clinical microbiology

authors： Brown SL,McKinney FT,Klein GC,Jones WL

更新日期：1980-02-01 00:00:00

abstract：:Genetic determinants of the invasive phenotype of Shigella spp. and enteroinvasive Escherichia coli (EIEC), two common agents of bacillary dysentery, are encoded on large (180- to 210 kilobase), nonconjugative plasmids. Several plasmid-encoded antigens have been implicated as important bacterial ligands that mediate t...

journal_title：Journal of clinical microbiology

authors： Venkatesan M,Buysse JM,Vandendries E,Kopecko DJ

更新日期：1988-02-01 00:00:00

abstract：:Monoclonal antibodies (MAb) were produced against the specific Bordetella pertussis antigen pertussis toxin (PT). In preliminary studies, one MAb (IB12) was selected and used in an enzyme-linked dot blot immunoassay to evaluate the ability of the method to detect known amounts of PT in control experiments and to test ...

journal_title：Journal of clinical microbiology

authors： Friedman RL,Paulaitis S,McMillan JW

更新日期：1989-11-01 00:00:00

abstract：:In this study, we determined the sequences of four intimin variant genes detected in attaching and effacing Escherichia coli isolates of human origin. Three of them were novel and were designated eae-eta (eta), eae-iota (iota), and eae-kappa (kappa). The fourth was identical to the recently described eae-zeta (zeta), ...

journal_title：Journal of clinical microbiology

authors： Zhang WL,Köhler B,Oswald E,Beutin L,Karch H,Morabito S,Caprioli A,Suerbaum S,Schmidt H

更新日期：2002-12-01 00:00:00

abstract：:A PCR method was developed to identify and fingerprint Candida krusei isolates simply and rapidly. The primer pair Arno1 and Arno2 was designed to amplify the polymorphic species-specific repetitive sequence CKRS-1 (C. krusei repeated sequence 1) that we identified in the nontranscribed intergenic regions (IGRs) of rR...

journal_title：Journal of clinical microbiology

authors： Carlotti A,Chaib F,Couble A,Bourgeois N,Blanchard V,Villard J

更新日期：1997-06-01 00:00:00

abstract：:A rapid dimethyl sulfoxide modification of an acid-fast technique was applied to direct fecal smears to monitor cryptosporidiosis in nonhuman primates. Brilliantly stained pink oocysts against a pale green background demonstrated well-preserved internal morphology and facilitated rapid, simple, noninvasive diagnosis w...

journal_title：Journal of clinical microbiology

authors： Bronsdon MA

更新日期：1984-06-01 00:00:00

abstract：:Serratia marcescens is a well-known cause of nosocomial infections and outbreaks, particularly in critically ill neonates and immunocompromised patients. Numerous methods have been proposed for typing. We used pulsed-field gel electrophoresis (PFGE) typing to analyze an outbreak in a neonatal intensive care unit (NICU...

journal_title：Journal of clinical microbiology

authors： Miranda G,Kelly C,Solorzano F,Leanos B,Coria R,Patterson JE

更新日期：1996-12-01 00:00:00