Characterization of rough and smooth morphotypes of Mycobacterium abscessus isolates from clinical specimens.

abstract：:The effects of standard fixatives (10% neutral buffered formalin, ethanol and mercury based) on the detection of Mycobacterium leprae DNA by the polymerase chain reaction (PCR) were studied. Mercury-based fixatives (Zenker's and Carnoy-Lebrun's fluids) strongly inhibited PCR amplification of M. leprae DNA. Ten percent...

journal_title：Journal of clinical microbiology

authors： Fiallo P,Williams DL,Chan GP,Gillis TP

更新日期：1992-12-01 00:00:00

abstract：:Beagle dogs inoculated intravenously with 1.75 x 10(9) viable Encephalitozoon cuniculi spores at 12.5 months of age were monitored for 18 months to assess infection in the adult dog. Parameters monitored included packed cell volume, total and differential leukocyte counts, and humoral and cellular responses to infecti...

journal_title：Journal of clinical microbiology

authors： Szabo JR,Shadduck JA

更新日期：1988-03-01 00:00:00

abstract：:Taeniasis due to Taenia solium is a disease with important public health consequences, since the larval stage is not exclusive to the animal intermediate, the pig, but also infects humans, causing neurocysticercosis. Early diagnosis and treatment of T. solium tapeworm carriers is important to prevent human cysticercos...

journal_title：Journal of clinical microbiology

authors： Mayta H,Gilman RH,Prendergast E,Castillo JP,Tinoco YO,Garcia HH,Gonzalez AE,Sterling CR,Cysticercosis Working Group in Peru.

更新日期：2008-01-01 00:00:00

abstract：:Reactivation of Epstein-Barr virus (EBV) in early human immunodeficiency virus (HIV) infection was investigated in 49 homosexual men who seroconverted to HIV (cases) as compared with 49 matched controls who remained seronegative to HIV during a longitudinal study. EBV infection was reactivated in cases 6 months, but n...

journal_title：Journal of clinical microbiology

authors： Rahman MA,Kingsley LA,Atchison RW,Belle S,Breinig MC,Ho M,Rinaldo CR Jr

更新日期：1991-06-01 00:00:00

abstract：:Fourth-generation human immunodeficiency virus (HIV) screening immunoassays reduce the diagnostic window between infection and diagnosis by the inclusion of HIV p24 antigen detection together with HIV antibody detection in the same test. We compared third- and fourth-generation HIV immunoassays and a dedicated HIV p24...

journal_title：Journal of clinical microbiology

authors： Speers D,Phillips P,Dyer J

更新日期：2005-10-01 00:00:00

abstract：:spa typing is a method used in molecular typing of methicillin-resistant Staphylococcus aureus (MRSA). Due to the lack of an amplification product in one of 55 MRSA cases, a new PCR setup, which allows an indicative description of a previously unknown type, was performed. ...

journal_title：Journal of clinical microbiology

authors： Reischauer M,Bernkopf M,Webersinke C

更新日期：2010-02-01 00:00:00

abstract：:A genomics-based PCR method was developed and used to test specimens from patients involved in a large outbreak of Mycoplasma pneumoniae in a closed religious community in New York State. New P1 adhesin gene primers were designed to bind to 9 of 10 target sequences in the repetitive-element sequences obtained from the...

journal_title：Journal of clinical microbiology

authors： Waring AL,Halse TA,Csiza CK,Carlyn CJ,Arruda Musser K,Limberger RJ

更新日期：2001-04-01 00:00:00

abstract：:A double-antibody (sandwich) enzyme-linked immunosorbent assay (ELISA) was adapted to detect Rift Valley fever virus antigen. Antibodies were purified from hyperimmune mouse and rabbit sera by affinity chromatography, using CNBr-activated Sepharose 4B coupled to a beta-propiolactone-inactivated sucrose-acetone-extract...

journal_title：Journal of clinical microbiology

authors： Niklasson B,Grandien M,Peters CJ,Gargan TP 2nd

更新日期：1983-06-01 00:00:00

abstract：:Bacteroides fragilis is an obligatory anaerobic, gram-negative bacterium found among the normal intestinal flora of humans. Enterotoxigenic strains of B. fragilis (ETBF) have been associated with diarrheal diseases in humans and animals. The enterotoxin of ETBF induces fluid changes in ligated intestinal segments and ...

journal_title：Journal of clinical microbiology

authors： Zhang G,Weintraub A

更新日期：1998-12-01 00:00:00

abstract：:We compared putative molecular markers of virulence (vacA, cagA, and iceA) of Helicobacter pylori strains isolated from 52 adult duodenal ulcer patients from West Bengal, India, with those of H. pylori strains isolated from 48 adult healthy volunteers from the same region. On the basis of genotyping by PCR, we conclud...

journal_title：Journal of clinical microbiology

authors： Chattopadhyay S,Datta S,Chowdhury A,Chowdhury S,Mukhopadhyay AK,Rajendran K,Bhattacharya SK,Berg DE,Nair GB

更新日期：2002-07-01 00:00:00

abstract：:A method of phage subtyping group A type 49 streptococci is described. The method is similar to that used for phage typing staphylococci, except that lysates obtained by induction with mitomycin C rather than propagated stock phages were used. Five type 49 strains were used as phage donors. Seventy-two strains of type...

journal_title：Journal of clinical microbiology

authors： Skjold SA,Wannamaker LW

更新日期：1976-09-01 00:00:00

abstract：:Sensitivity of the enzyme-linked immunosorbent assay for detecting serum antibodies to rotavirus was greatly enhanced when rotavirus particles were fragmented by chaotropic agents (NaSCN or guanidine hydrochloride) before adsorption of the antigens to the solid phase. For detecting fecal antibodies, the addition of fe...

journal_title：Journal of clinical microbiology

authors： Inouye S,Matsuno S,Yamaguchi H

更新日期：1984-02-01 00:00:00

abstract：:The aim of this study was to find a reliable method for the detection and identification of fungi in fungus balls of the maxillary sinus and to evaluate the spectrum of fungi in these samples. One hundred twelve samples were obtained from patients with histologically proven fungal infections; 81 samples were paraffin-...

journal_title：Journal of clinical microbiology

authors： Willinger B,Obradovic A,Selitsch B,Beck-Mannagetta J,Buzina W,Braun H,Apfalter P,Hirschl AM,Makristathis A,Rotter M

更新日期：2003-02-01 00:00:00

abstract：:This study used a nested multiplex PCR method to detect three periodontal pathogens in subgingival plaque collected before treatment and at 2 and 6 months posttreatment from 107 patients with severe, generalized periodontitis. The proportions of the patients who harbored these bacteria before periodontal treatment wer...

journal_title：Journal of clinical microbiology

authors： Ready D,D'Aiuto F,Spratt DA,Suvan J,Tonetti MS,Wilson M

更新日期：2008-10-01 00:00:00

abstract：:It has been difficult to obtain pure Pneumocystis carinii antigen either from cultures or from infected lungs for use in producing a specific antibody against P. carinii. This report describes an approach toward producing a monoclonal antibody that bypasses the antigen purification steps. P. carinii infection was deve...

journal_title：Journal of clinical microbiology

authors： Lee CH,Bolinger CD,Bartlett MS,Kohler RB,Wilde CE 3rd,Smith JW

更新日期：1986-03-01 00:00:00

abstract：:In order to compare methods for subtyping Neisseria meningitidis serogroup B isolates, 96 isolates obtained from various locations in the United States and northwestern Europe were subtyped by five methods: monoclonal antibody (MAb)-based serotyping and serosubtyping, DNA macrorestriction analysis by pulsed-field gel ...

journal_title：Journal of clinical microbiology

authors： Swaminathan B,Matar GM,Reeves MW,Graves LM,Ajello G,Bibb WF,Helsel LO,Morales M,Dronavalli H,el-Swify M,DeWitt W,Hunter SB

更新日期：1996-06-01 00:00:00

abstract：:Transmissible spongiform encephalopathy strains can be differentiated by their behavior in bioassays and by molecular analyses of the disease-associated prion protein (PrP) in a posttranslationally transformed conformation (PrPSc). Until recently, isolates from cases of bovine spongiform encephalopathy (BSE) appeared ...

journal_title：Journal of clinical microbiology

authors： Jacobs JG,Langeveld JP,Biacabe AG,Acutis PL,Polak MP,Gavier-Widen D,Buschmann A,Caramelli M,Casalone C,Mazza M,Groschup M,Erkens JH,Davidse A,van Zijderveld FG,Baron T

更新日期：2007-06-01 00:00:00

abstract：:Reference isolates of Mycobacterium neoaurum, Mycobacterium aurum, and the nonvalidated species "Mycobacterium lacticola" were the focus of two recent molecular taxonomic studies. On the basis of this grouping, we identified 46 clinical pigmented, rapidly growing mycobacterial isolates. By 16S rRNA gene sequencing, on...

journal_title：Journal of clinical microbiology

authors： Brown-Elliott BA,Wallace RJ Jr,Petti CA,Mann LB,McGlasson M,Chihara S,Smith GL,Painter P,Hail D,Wilson R,Simmon KE

更新日期：2010-12-01 00:00:00

abstract：:Multidrug-resistant (MDR) Klebsiella pneumoniae is one of the most important causes of nosocomial infections worldwide. After the spread of strains resistant to beta-lactams at the end of the previous century, the diffusion of isolates resistant to carbapenems and colistin is now reducing treatment options and the con...

journal_title：Journal of clinical microbiology

authors： Onori R,Gaiarsa S,Comandatore F,Pongolini S,Brisse S,Colombo A,Cassani G,Marone P,Grossi P,Minoja G,Bandi C,Sassera D,Toniolo A

更新日期：2015-09-01 00:00:00

abstract：:Culture of autologous CD4 lymphocytes from peripheral blood mononuclear cells compared favorably with two other methods for the measurement of cell-associated human immunodeficiency virus type 1 (HIV-1). For subjects with undetectable HIV-1 RNA levels in plasma, there was a 10,000-fold range of cell-associated virus d...

journal_title：Journal of clinical microbiology

authors： Tremblay CL,Giguel F,Merrill DP,Wong JT,Rosenberg E,Kalams S,Walker BD,D'Aquila RT,Hirsch MS

更新日期：2000-11-01 00:00:00

abstract：:A sensitive detection system specific for Legionella pneumophila in water was developed. This system is based on amplification of a chromosomal DNA sequence from L. pneumophila by the polymerase chain reaction, followed by detection of the amplified product by hybridization of a radiolabeled oligodeoxynucleotide. Afte...

journal_title：Journal of clinical microbiology

authors： Starnbach MN,Falkow S,Tompkins LS

更新日期：1989-06-01 00:00:00

abstract：:Staphylococcus epidermidis, a human commensal, is a common cause of bacteremia in immunocompromised patients with indwelling medical devices. We report a case of isolated cervical adenitis caused by S. epidermidis in an immunocompetent patient and comment on the presumed pathogenesis. ...

journal_title：Journal of clinical microbiology

authors： Ryan-Poirier K,Patrick CC

更新日期：1993-02-01 00:00:00

abstract：:Forty-eight clinical Acinetobacter isolates with different epidemic behavior were investigated for the presence of integrons and plasmids and for antibiotic susceptibility. Integrons were demonstrated in 50% of the strains by an integrase gene PCR. Epidemic strains of Acinetobacter baumannii were found to contain sign...

journal_title：Journal of clinical microbiology

authors： Koeleman JG,Stoof J,Van Der Bijl MW,Vandenbroucke-Grauls CM,Savelkoul PH

更新日期：2001-01-01 00:00:00

abstract：:The comparison of Gram-stained urethral smears with Gen-Probe for the detection of Neisseria Gonorrhoeae in the urethras of males with symptomatic urethritis revealed a 99.6% correlation between the two methods. A simple Gram stain would appear to be the method of choice for the detection of gonorrhea in symptomatic m...

journal_title：Journal of clinical microbiology

authors： Juchau SV,Nackman R,Ruppart D

更新日期：1995-11-01 00:00:00

abstract：:Approximately 15 to 35% of patients with a first episode of Clostridium difficile-associated diarrhea relapse within 2 months. Between 1994 and 1997, strains from 93 hospitalized patients with C. difficile recurrences were fingerprinted by using both serotyping and PCR-ribotyping. The results showed that 48.4% of clin...

journal_title：Journal of clinical microbiology

authors： Barbut F,Richard A,Hamadi K,Chomette V,Burghoffer B,Petit JC

更新日期：2000-06-01 00:00:00

abstract：:Three hundred sixty Enterobacteriaceae and nonfermenting gram-negative bacilli, isolated during one week in May 2004 at five hospitals in Amsterdam, The Netherlands, were evaluated for the presence of extended-spectrum beta-lactamases (ESBLs). A prevalence of 7.8% was found, in contrast to the 1% observed in 1997. CTX...

journal_title：Journal of clinical microbiology

authors： al Naiemi N,Bart A,de Jong MD,Vandenbroucke-Grauls CM,Rietra PJ,Debets-Ossenkopp YJ,Wever PC,Spanjaard L,Bos AJ,Duim B

更新日期：2006-08-01 00:00:00

abstract：:Candida samples were taken over a period of 2 years from 54 human immunodeficiency virus (HIV)-positive asymptomatic subjects to evaluate changes in yeast carriage, intensity of carriage, and genotype over time. Overall, we found that HIV-positive patients with CD4(+)-cell counts of between 200 and 400/microl had sign...

journal_title：Journal of clinical microbiology

authors： Vargas KG,Joly S

更新日期：2002-02-01 00:00:00

abstract：:The type strains of six Campylobacter species or subspecies were examined in eight tests used for the identification of such organisms. False-positive results were obtained in certain tests (growth on 1% bile, brilliant green, selenite, trimethylamine-N-oxide, 2,3,5-triphenyl tetrazolium chloride, and minimal media) w...

journal_title：Journal of clinical microbiology

authors： On SL,Holmes B

更新日期：1991-05-01 00:00:00

abstract：:The etiology of Crohn's disease in humans is largely unknown. Clinical signs of Crohn's disease partly resemble the clinical picture of Johne's disease in ruminants caused by Mycobacterium avium subsp. paratuberculosis. Because of the high prevalence of these bacteria in (products of) ruminants and their remarkable th...

journal_title：Journal of clinical microbiology

authors： Overduin P,Schouls L,Roholl P,van der Zanden A,Mahmmod N,Herrewegh A,van Soolingen D

更新日期：2004-11-01 00:00:00

abstract：:We developed a simple, rapid, high-throughput detection and genotyping method for noroviruses using real-time reverse transcription-PCR (RT-PCR) and high-resolution melting (HRM) analysis to create a difference plot. The capsid gene was amplified by real-time RT-PCR in the presence of ResoLight HRM dye, a saturating D...

journal_title：Journal of clinical microbiology

authors： Tajiri-Utagawa E,Hara M,Takahashi K,Watanabe M,Wakita T

更新日期：2009-02-01 00:00:00