Comparison of Gram stain with DNA probe for detection of Neisseria gonorrhoeae in urethras of symptomatic males.

abstract：:Fluorophore-assisted carbohydrate electrophoresis (FACE) is a straightforward, sensitive method for determining the presence and relative abundance of individual oligomannosyl residues in Candida mannoprotein, the major antigenic determinant located on the outer surface of the yeast cell wall. The single terminal alde...

journal_title：Journal of clinical microbiology

authors： Goins TL,Cutler JE

更新日期：2000-08-01 00:00:00

abstract：:INNO-LiPA and PapType human papillomavirus (HPV) genotyping assays were compared for detection of HPV genotypes on archival vulvar tissue. The INNO-LiPA assay detected 49 HPV-16 infections, compared with 47 detected by the PapType assay. The INNO-LiPA assay detected amplifiable DNA in 59 (91%) biopsy specimens, compar...

journal_title：Journal of clinical microbiology

authors： Tan SE,Garland SM,Rumbold AR,Tabrizi SN

更新日期：2011-11-01 00:00:00

abstract：:A 128-member specimen pooling scheme for acute HIV infection (AHI) detection was evaluated using 21 AHI specimens (range, 1,520 to 500,000 copies/ml) previously identified by RNA testing of 16-member plasma pools. HIV-1 RNA was detectable in 128-member pools for all 21 specimens; however, one pool created from a speci...

journal_title：Journal of clinical microbiology

authors： Sullivan TJ,Patel P,Hutchinson A,Ethridge SF,Parker MM

更新日期：2011-10-01 00:00:00

abstract：:Infectious gastroenteritis is one of the most common diseases in young children. To clarify the infectious etiology of diarrhea in Danish children less than 5 years of age, we conducted a 2-year prospective case-control study. Stools from 424 children with diarrhea and 870 asymptomatic age-matched controls were examin...

journal_title：Journal of clinical microbiology

authors： Olesen B,Neimann J,Böttiger B,Ethelberg S,Schiellerup P,Jensen C,Helms M,Scheutz F,Olsen KE,Krogfelt K,Petersen E,Mølbak K,Gerner-Smidt P

更新日期：2005-08-01 00:00:00

abstract：:Cryptococcus neoformans var. neoformans (serotype D) and C. neoformans var. grubii (serotype A) differ in geographic prevalence and dermatotropism, with C. neoformans var. neoformans strains being more prevalent among isolates from temperate countries as well as from skin infections. Analysis of 19 strains from each s...

journal_title：Journal of clinical microbiology

authors： Martinez LR,Garcia-Rivera J,Casadevall A

更新日期：2001-09-01 00:00:00

abstract：:We compared serial measurements of antibodies to mannan and to a cytoplasmic antigen (enzyme-linked immunosorbent assays), detection of mannan and an unidentified candidal antigen (latex agglutination), and assays of mannose and arabinitol (gas chromatographic assay of per-O-acetylated aldonitrile derivatives). In a h...

journal_title：Journal of clinical microbiology

authors： Greenfield RA,Troutt DL,Rickard RC,Altmiller DH

更新日期：1988-03-01 00:00:00

abstract：:Early and accurate diagnosis is essential for optimal therapeutic outcomes in patients infected with HIV. Currently, none of the commercially available fourth-generation assays differentiate HIV-1 and HIV-2 antibodies (Ab) or the HIV-1 p24 antigen (Ag). The aim of this study was to evaluate the performance of a novel ...

journal_title：Journal of clinical microbiology

authors： Salmona M,Delarue S,Delaugerre C,Simon F,Maylin S

更新日期：2014-01-01 00:00:00

abstract：:The Kodak SureCell Herpes Test Kit (SC-HSV) was evaluated in a high-risk sexually transmitted disease clinic population. A total of 97 lesion specimens (from 94 patients) were tested for the presence of herpes simplex virus by SC-HSV and by spin-amplified tissue culture confirmed by an enzyme immunoassay. The overall ...

journal_title：Journal of clinical microbiology

authors： Dorian KJ,Beatty E,Atterbury KE

更新日期：1990-09-01 00:00:00

abstract：:A DNA microarray platform, based on the nucleotide sequences of the internal transcribed spacer regions (ITS1 and ITS2) of the rRNA gene, was developed to identify 32 fungal pathogens at the species level. The probe sequences were spotted onto polycarbonate slides with a mini-microarray printer, and after the hybridiz...

journal_title：Journal of clinical microbiology

authors： Sturaro LL,Gonoi T,Busso-Lopes AF,Tararam CA,Levy CE,Lyra L,Trabasso P,Schreiber AZ,Kamei K,Moretti ML

更新日期：2018-04-25 00:00:00

abstract：:Fifteen monoclonal antibodies (MAbs) made to coxsackievirus B-3 were tested against a panel of enteroviruses by indirect immunofluorescence. The MAbs included seven which reacted with coxsackievirus B-3 only, five which reacted with more than one enterovirus included in the panel, one which reacted with broad enterovi...

journal_title：Journal of clinical microbiology

authors： Yagi S,Schnurr D,Lin J

更新日期：1992-09-01 00:00:00

abstract：:Recent reports of natural paratuberculosis (or Johne's disease) in rabbits, foxes, and stoats has focused debate on the presence and importance of wildlife reservoirs in the epidemiology of this disease. This paper describes an extensive study investigating 18 nonruminant wildlife species for evidence of paratuberculo...

journal_title：Journal of clinical microbiology

authors： Beard PM,Daniels MJ,Henderson D,Pirie A,Rudge K,Buxton D,Rhind S,Greig A,Hutchings MR,McKendrick I,Stevenson K,Sharp JM

更新日期：2001-04-01 00:00:00

abstract：:The medium described is a simple yeast extract broth capable of growing large number of Legionella neumophila, the causative organism of Legionnaires disease. Filtration was chosen as a means of sterilization, since medium that was autoclaved did not support growth without the presence of Norite A. The filtered medium...

journal_title：Journal of clinical microbiology

authors： Ristroph JD,Hedlund KW,Allen RG

更新日期：1980-01-01 00:00:00

abstract：:Comparison of nucleotide sequences of the partial 1D region of foot-and-mouth disease type C viruses of Indian origin with those of European, South American, and Southeast Asian viruses revealed that the Indian viruses form a distinct genotype. The vaccine strain C IND/51/79 belongs to this genotype and may be a proto...

journal_title：Journal of clinical microbiology

authors： Nagendrakumar SB,Reddy GS,Chandran D,Thiagarajan D,Rangarajan PN,Srinivasan VA

更新日期：2005-02-01 00:00:00

abstract：:We compared the coreceptor tropism-predicting performance of a specific genotypic algorithm for HIV-1 subtype D and that of the geno2pheno algorithm with different cutoffs. The D-specific algorithm and geno2pheno with a false-positivity rate cutoff of 2.5% had the same concordance with the phenotypic determination. Th...

journal_title：Journal of clinical microbiology

authors： Dina J,Raymond S,Maillard A,Le Guillou-Guillemette H,Rodalec A,Beby-Defaux A,Giraudeau G,Vallet S,Mourez T,Payan C,Vabret A,Ruffault A,Ferre V,Izopet J,Plantier JC

更新日期：2013-09-01 00:00:00

abstract：:The virulence plasmid of Escherichia coli O157 strain EDL933 carries a 10-kb putative virulence gene designated toxB. Little is known about the distribution of this gene among E. coli O157 strains or its presence in other enterohemorrhagic E. coli (EHEC) and enteropathogenic E. coli (EPEC) strains. We developed PCR an...

journal_title：Journal of clinical microbiology

authors： Tozzoli R,Caprioli A,Morabito S

更新日期：2005-08-01 00:00:00

abstract：:We compared examination of unstained and Gram-stained smears as methods of evaluating sputum quality. Of 100 slides examined, 96 (96%) were graded identically. Each discrepancy between methods was near the cutoff for specimen acceptability. We concluded that examination of unstained smears can provide an accurate, cos...

journal_title：Journal of clinical microbiology

authors： Wetterau LM,Zeimis RT,Hollick GE

更新日期：1986-07-01 00:00:00

abstract：:While high-copy-number IS6110-based restriction fragment length polymorphism (HCN-RFLP) is the gold standard for typing most Mycobacterium tuberculosis strains, the time taken for culturing and low throughput make it impractical for large-scale prospective typing of large numbers of isolates. The development of a new ...

journal_title：Journal of clinical microbiology

authors： Gopaul KK,Brown TJ,Gibson AL,Yates MD,Drobniewski FA

更新日期：2006-07-01 00:00:00

abstract：:The API 20S system (Analytab Products, Plainview, N.Y.) was evaluated for the rapid identification of multiply antibiotic-resistant aerobic diphtheroids. Sixty-eight clinical isolates of multiply resistant Centers for Disease Control group JK and group D2 corynebacteria had API 20S profiles which were clearly differen...

journal_title：Journal of clinical microbiology

authors： Kelly MC,Smith ID,Anstey RJ,Thornley JH,Rennie RP

更新日期：1984-02-01 00:00:00

abstract：:Two new T-cell-based reporter cell lines were established to measure human immunodeficiency virus type 1 (HIV-1) infectivity. One cell line naturally expresses CD4 and CXCR4, making it susceptible to X4-tropic viruses, and the other cell line, in which a CCR5 expression vector was introduced, is susceptible to both X4...

journal_title：Journal of clinical microbiology

authors： Chiba-Mizutani T,Miura H,Matsuda M,Matsuda Z,Yokomaku Y,Miyauchi K,Nishizawa M,Yamamoto N,Sugiura W

更新日期：2007-02-01 00:00:00

abstract：:Serological interrelations among 46 strains of Haemophilus somnus of various geographical and pathological origins were studied by agglutination and agglutinin adsorption procedures. Sera prepared against nine representative American and Swiss isolates agglutinated all other strains to various titers. Adsorption of an...

journal_title：Journal of clinical microbiology

authors： Canto GJ,Biberstein EL

更新日期：1982-06-01 00:00:00

abstract：:The ability to rapidly diagnose influenza virus infections is of the utmost importance in the evaluation of patients with upper respiratory tract infections. It is also important for the influenza surveillance activities performed by national influenza centers. In the present study we modified a multiplex real-time re...

journal_title：Journal of clinical microbiology

authors： Hindiyeh M,Levy V,Azar R,Varsano N,Regev L,Shalev Y,Grossman Z,Mendelson E

更新日期：2005-02-01 00:00:00

abstract：:Pneumocystis jirovecii pneumonia (PcP) is a major cause of respiratory illness in patients with AIDS. The identification of multiple single-nucleotide polymorphisms (SNPs) at three distinct P. jirovecii loci encoding dihydrofolate reductase (DHFR), mitochondrial large-subunit rRNA (mtLSU rRNA), and superoxide dismutas...

journal_title：Journal of clinical microbiology

authors： Esteves F,Gaspar J,De Sousa B,Antunes F,Mansinho K,Matos O

更新日期：2011-05-01 00:00:00

abstract：:Although screening for maternal toxoplasmic seroconversion during pregnancy is based on immunodiagnostic assays, the diagnosis of clinically relevant toxoplasmosis greatly relies upon molecular methods. A problem is that this molecular diagnosis is subject to variation of performances, mainly due to a large diversity ...

journal_title：Journal of clinical microbiology

authors： Sterkers Y,Varlet-Marie E,Cassaing S,Brenier-Pinchart MP,Brun S,Dalle F,Delhaes L,Filisetti D,Pelloux H,Yera H,Bastien P

更新日期：2010-09-01 00:00:00

abstract：:This collaborative three-center study evaluated NCCLS M38-A document testing conditions and other testing conditions for the antifungal susceptibility testing of 35 isolates of Aspergillus nidulans, A. terreus, Bipolaris hawaiiensis, B. spicifera, Cladophialophora bantiana, Dactylaria constricta, Fusarium solani, Paec...

journal_title：Journal of clinical microbiology

authors： Espinel-Ingroff A,Chaturvedi V,Fothergill A,Rinaldi MG

更新日期：2002-10-01 00:00:00

abstract：:We studied the evolution of Vibrio cholerae O1 during the 1991 to 2000 cholera epidemic in Mexico by biochemical, serological, and molecular characterization of strains collected during this period. Strains were divided into toxigenic and nontoxigenic groups according to the presence or absence of genes encoding chole...

journal_title：Journal of clinical microbiology

authors： Lizárraga-Partida ML,Quilici ML

更新日期：2009-05-01 00:00:00

abstract：:The QuantiFERON-TB Gold Plus (QFT-Plus; Qiagen, Germantown, MD) interferon gamma release assay (IGRA) received FDA clearance in 2017 and will replace the prior version of the assay, the QFT-Gold In-Tube (QFT-GIT). Here, we compared performances of the QFT-Plus assay and the QFT-GIT version in a diverse patient populat...

journal_title：Journal of clinical microbiology

authors： Theel ES,Hilgart H,Breen-Lyles M,McCoy K,Flury R,Breeher LE,Wilson J,Sia IG,Whitaker JA,Clain J,Aksamit TR,Escalante P

更新日期：2018-06-25 00:00:00

abstract：:We studied the use of dried spots of bodily fluids (plasma, whole blood, and mother's milk) on filter paper as a means of sample collection and storage for human immunodeficiency virus type 1 (HIV-1) viral load testing under stringent field conditions. Plasma placed directly in lysis buffer, which is customarily used ...

journal_title：Journal of clinical microbiology

authors： Ayele W,Schuurman R,Messele T,Dorigo-Zetsma W,Mengistu Y,Goudsmit J,Paxton WA,de Baar MP,Pollakis G

更新日期：2007-03-01 00:00:00

abstract：:The epidemiology of new acquisition of antibiotic-resistant organisms (AROs) in community-based skilled nursing facilities (SNFs) is not well studied. To define the incidence, persistence of, and time to new colonization with methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococci (VRE), a...

journal_title：Journal of clinical microbiology

authors： Fisch J,Lansing B,Wang L,Symons K,Cherian K,McNamara S,Mody L

更新日期：2012-05-01 00:00:00

abstract：:Tropheryma whipplei is the causative agent of classic Whipple's disease (WD) and other clinical entities, such as localized infection. Asymptomatic carriers have also been reported, mainly based on the testing of fecal samples. Our objective was to undertake a retrospective analysis of molecular biology usage for the ...

journal_title：Journal of clinical microbiology

authors： Edouard S,Fenollar F,Raoult D

更新日期：2012-12-01 00:00:00

abstract：:A simple enzyme-linked immunosorbent assay was developed for the identification of cultured mycobacteria belonging to the Mycobacterium tuberculosis complex, the Mycobacterium avium complex, and Mycobacterium kansasii. Six monoclonal antibodies were used: two (F23-49 and F24-2) were specific for the M. tuberculosis co...

journal_title：Journal of clinical microbiology

authors： Schöningh R,Verstijnen CP,Kuijper S,Kolk AH

更新日期：1990-04-01 00:00:00