Liquid medium for growth of Legionella pneumophila.

abstract：:The use of molecular identification techniques has revealed an increasing number of new species within Aspergillus section Terrei. We phenotyped a set of 26 clinical isolates that showed genetic differences from Aspergillus terreus sensu stricto by analyzing sequences from PCR-amplified β-tubulin and calmodulin genes ...

journal_title：Journal of clinical microbiology

authors： Guinea J,Sandoval-Denis M,Escribano P,Peláez T,Guarro J,Bouza E

更新日期：2015-02-01 00:00:00

abstract：:Pasteurella multocida is a small nonmotile gram-negative coccobacillus that is found in the nasopharynx and gastrointestinal tract of many wild and domesticated animals. In humans it most commonly causes cellulitis and localized superficial skin abscesses following an animal bite or scratch. The respiratory tract is t...

journal_title：Journal of clinical microbiology

authors： Chen HI,Hulten K,Clarridge JE 3rd

更新日期：2002-09-01 00:00:00

abstract：:Klebsiella pneumoniae has been emerging as the leading cause of liver abscess in diabetic patients. Results of molecular typing of K. pneumoniae isolates from two siblings with liver abscess, their family members, and the environment suggest a possibility of cross infection of liver abscess by the fecal-oral route wit...

journal_title：Journal of clinical microbiology

authors： Chiu CH,Su LH,Wu TL,Hung IJ

更新日期：2001-06-01 00:00:00

abstract：:Diagnosis of invasive aspergillosis (IA) requires increasingly rapid molecular methods that enable sensitive detection and discrimination between species. We designed and evaluated a real-time PCR-based method that combined melting temperature (T(m)) calling analysis of a specific probe with high-resolution melting an...

journal_title：Journal of clinical microbiology

authors： Alonso M,Escribano P,Guinea J,Recio S,Simon A,Peláez T,Bouza E,García de Viedma D

更新日期：2012-10-01 00:00:00

abstract：:Pyrazinamide is important in tuberculosis treatment, as it is bactericidal to semidormant mycobacteria not killed by other antituberculosis drugs. Pyrazinamide is also one of the cornerstone drugs retained in the treatment of multidrug-resistant tuberculosis (MDR-TB). However, due to technical difficulties, routine dr...

journal_title：Journal of clinical microbiology

authors： Mphahlele M,Syre H,Valvatne H,Stavrum R,Mannsåker T,Muthivhi T,Weyer K,Fourie PB,Grewal HM

更新日期：2008-10-01 00:00:00

abstract：:Mucosal and systemic immunologic recognition of cagA by Helicobacter pylori-infected individuals is associated with peptic ulcer disease; however, in the laboratory, expression of cagA is subject to artificial conditions which may not accurately reflect the conditions in host tissues. Gastric antral and body biopsy sp...

journal_title：Journal of clinical microbiology

authors： Peek RM Jr,Miller GG,Tham KT,Pérez-Pérez GI,Cover TL,Atherton JC,Dunn GD,Blaser MJ

更新日期：1995-01-01 00:00:00

abstract：:For recovery of brucellae from clinical specimens, a lysis-concentration procedure was superior to the procedure of Castañeda (M. R. Castañeda, Proc. Soc. Exp. Biol. Med. 64:114-115, 1947). ...

journal_title：Journal of clinical microbiology

authors： Etemadi H,Raissadat A,Pickett MJ,Zafari Y,Vahedifar P

更新日期：1984-09-01 00:00:00

abstract：:We determined the E-Test and National Committee for Clinical Laboratory Standards standardized agar dilution MICs of ceftazidime, ciprofloxacin, piperacillin, and tobramycin for Pseudomonas aeruginosa during tests of 100 rough and mucoid P. aeruginosa isolates from cystic fibrosis patients. The levels of agreement (+/...

journal_title：Journal of clinical microbiology

authors： Marley EF,Mohla C,Campos JM

更新日期：1995-12-01 00:00:00

abstract：:We reviewed laboratory-acquired infections occurring in Utah from 1978 through 1982. Written and telephone interviews of supervisors of 1,191 laboratorians revealed an estimated annual incidence of 3 laboratory-acquired infections per 1,000 employees. Infections, in order of frequency, included hepatitis B (clinical c...

journal_title：Journal of clinical microbiology

authors： Jacobson JT,Orlob RB,Clayton JL

更新日期：1985-04-01 00:00:00

abstract：:Triplicate preserved fecal samples from 265 patients were pooled into single samples, and the recovery rate of intestinal parasites from the pooled samples was compared with that from the individual samples. Parasites were detected in 109 patients when results from the individual samples were used compared with 108 pa...

journal_title：Journal of clinical microbiology

authors： Aldeen WE,Shisenant J,Hale D,Matsen J,Carroll K

更新日期：1993-01-01 00:00:00

abstract：:Two group A, subgroup I, rotavirus strains possessing "super short" RNA patterns were adapted to growth in MA-104 cells. Both produced marked cytopathic effect in primary culture. Reciprocal cross-neutralization titers, polypeptide analysis, and the serum neutralizing antibody response of an infected child suggested t...

journal_title：Journal of clinical microbiology

authors： Albert MJ,Unicomb LE,Bishop RF

更新日期：1987-01-01 00:00:00

abstract：:Forested areas adjacent to Milwaukee, Wis., and Chicago, Ill., were investigated for rodents and ticks infected with Borrelia burgdorferi, the causative agent of Lyme disease. White-footed mice (Peromyscus leucopus or Peromyscus maniculatus), meadow voles (Microtus pennsylvanicus), and eastern chipmunks (Tamias striat...

journal_title：Journal of clinical microbiology

authors： Callister SM,Nelson JA,Schell RF,Jobe DA,Bautz R,Agger WA,Coggins J

更新日期：1991-02-01 00:00:00

abstract：:The development of a rhinovirus (RV)-RNA-specific reverse transcription (RT)-PCR assay is complicated by the close homology between the RV and enterovirus (EV) genomes in the highly conserved 5'-noncoding region, which is chosen for primer design in most RT-PCR assays. We have developed a sensitive, rapid, and RV-spec...

journal_title：Journal of clinical microbiology

authors： Steininger C,Aberle SW,Popow-Kraupp T

更新日期：2001-01-01 00:00:00

abstract：:Herpes infections are among the most common sexually transmitted diseases and are the most common cause of genital ulcer disease in the United States. This study addresses the changing distribution of herpes simplex virus type 1 (HSV-1) and HSV-2 in patients presenting for evaluation of herpetic infections. Viral cult...

journal_title：Journal of clinical microbiology

authors： Ribes JA,Steele AD,Seabolt JP,Baker DJ

更新日期：2001-09-01 00:00:00

abstract：:This case-control study detected and characterized Shigella and diarrheagenic Escherichia coli (DEC) types among Vietnamese children less than 5 years old. In 249 children with diarrhea and 124 controls, Shigella spp. was an important cause of diarrhea (P < 0.05). We used multiplex PCR and DNA probes to detect enteroi...

journal_title：Journal of clinical microbiology

authors： Hien BT,Scheutz F,Cam PD,Serichantalergs O,Huong TT,Thu TM,Dalsgaard A

更新日期：2008-03-01 00:00:00

abstract：:Inactivation of penicillin and gentamicin in cultures was achieved by using monoclonal antibodies against these antibiotics. A viridans group streptococcus (penicillin MIC, less than or equal to 0.06 micrograms/ml) and Escherichia coli ATCC 35218 (gentamicin MIC, less than or equal to 1 microgram/ml) were able to grow...

journal_title：Journal of clinical microbiology

authors： Sierra-Madero JG,Caulfield MJ,Hall GS,Washington JA

更新日期：1988-09-01 00:00:00

abstract：:Campylobacter jejuni (20 strains) and Campylobacter coli (12 strains) were assigned to four biovars for each species based on phenotypic tests that were easy to perform and interpret. The resulting biotyping schemes offer a greater degree of distinction among C. jejuni and C. coli strains than any of the other biotypi...

journal_title：Journal of clinical microbiology

authors： Roop RM 2nd,Smibert RM,Krieg NR

更新日期：1984-11-01 00:00:00

abstract：:In a prospective, multicenter study of 342 blood samples from 187 patients with systemic inflammatory response syndrome, sepsis, or neutropenic fever, a new commercial PCR test (SepsiTest; Molzym) was evaluated for rapid diagnosis of bacteremia. The test comprises a universal PCR from the 16S rRNA gene, with subsequen...

journal_title：Journal of clinical microbiology

authors： Wellinghausen N,Kochem AJ,Disqué C,Mühl H,Gebert S,Winter J,Matten J,Sakka SG

更新日期：2009-09-01 00:00:00

abstract：:By the use of enzyme immunoassay and RNA electrophoresis, rotavirus serotyping was performed with stool specimens from 605 children. Serotypes 1, 2, 3, and 4 accounted for 63, 9, 9, and 19% of the cases, respectively, with considerable yearly variation. An average of three serotypes cocirculated each year. ...

journal_title：Journal of clinical microbiology

authors： Begue RE,Dennehy PH,Huang J,Martin P

更新日期：1992-06-01 00:00:00

abstract：:Ureaplasma urealyticum has been associated with urethritis in men, obstetric problems in women, and respiratory distress syndrome in preterm infants. U. urealyticum can be divided into two biovars comprising 14 serovars. Partial sequences of genes encoding the multiple-banded antigens of the cell surface are known. Us...

journal_title：Journal of clinical microbiology

authors： Pitcher D,Sillis M,Robertson JA

更新日期：2001-05-01 00:00:00

abstract：:Leptospira is a highly diverse genus comprising many species and serogroups in Brazil as well as all over the world. However, a study by arbitrarily primed PCR of 44 leptospiral strains isolated from humans during three different outbreaks in Brazilian urban centers reveals that 43 of 44 isolates exhibit very similar ...

journal_title：Journal of clinical microbiology

authors： Pereira MM,Matsuo MG,Bauab AR,Vasconcelos SA,Moraes ZM,Baranton G,Saint Girons I

更新日期：2000-01-01 00:00:00

abstract：:Identification of chromosomal markers for rapid detection of Bacillus anthracis is difficult because significant chromosomal homology exists among B. anthracis, Bacillus cereus, and Bacillus thuringiensis. We evaluated the bacterial gyrA gene as a potential chromosomal marker for B. anthracis. A real-time PCR assay wa...

journal_title：Journal of clinical microbiology

authors： Hurtle W,Bode E,Kulesh DA,Kaplan RS,Garrison J,Bridge D,House M,Frye MS,Loveless B,Norwood D

更新日期：2004-01-01 00:00:00

abstract：:A cell-free growth medium for the opportunistic pathogenic ameba Balamuthia mandrillaris is presented. This represents an advance over the use of monkey kidney cells for growth of the amebas and can be helpful in isolation of these amebas from brain tissue from cases in which amebic meningoencephalitis is a diagnostic...

journal_title：Journal of clinical microbiology

authors： Schuster FL,Visvesvara GS

更新日期：1996-02-01 00:00:00

abstract：:Phialemonium obovatum was found to be the cause of osteomyelitis in a German shepherd dog. Histologic examination of the biopsied material from the left tibia revealed septate, irregularly branched hyphae, swollen cells, and ovate-to-spherical cells divided by a transverse septum. The majority of the fungal elements w...

journal_title：Journal of clinical microbiology

authors： Lomax LG,Cole JR,Padhye AA,Ajello L,Chandler FW,Smith BR

更新日期：1986-05-01 00:00:00

abstract：:A simple method of storage and retrieval of serum samples for adenovirus serology is described. Complement-fixing antibodies remained stable for at least 5 years when stored at -10 degrees C or below. ...

journal_title：Journal of clinical microbiology

authors： Edwards EA

更新日期：1977-02-01 00:00:00

abstract：:We evaluated a commercially available second-generation anti-H. pylori immunoglobulin G enzyme immunoassay (EIA) (Cobas Core Anti-Helicobacter pylori EIA; Roche S. A., Basel, Switzerland) for serodiagnosis of H. pylori infection. The results of the assay were assessed in relation to the results of bacterial culture, u...

journal_title：Journal of clinical microbiology

authors： Goossens H,Glupczynski Y,Burette A,Van den Borre C,Butzler JP

更新日期：1992-01-01 00:00:00

abstract：:Adolescent children were surveyed for colonization with Mycoplasma hominis and Ureaplasma urealyticum by culturing urine specimens. Rates were compared between three study groups: (i) 397 children attending parochial schools, (ii) 293 children attending an adolescent clinic specializing in adjustment problems, and (ii...

journal_title：Journal of clinical microbiology

authors： Foy H,Kenny G,Bor E,Hammar S,Hickman R

更新日期：1975-09-01 00:00:00

abstract：:GB virus C (GBV-C) markers were analyzed in two to three generations in three families with documented vertical transmission of GBV-C. None of the maternal grandparents had GBV-C markers, whereas the male spouses had GBV-C envelope 2 antibodies. Evidence was found for intrafamilial transmission but not for GBV-C trans...

journal_title：Journal of clinical microbiology

authors： Chen M,Fischler B,Hultgren C,Halasz R,Nemeth A,Sällberg M

更新日期：1999-12-01 00:00:00

abstract：:The Triage parasite panel (BIOSITE Diagnostics, San Diego, Calif.) is a new qualitative enzyme immunoassay (EIA) panel for the detection of Giardia lamblia, Entamoeba histolytica/E. dispar, and Cryptosporidium parvum in fresh or fresh, frozen, unfixed human fecal specimens. By using specific antibodies, antigens speci...

journal_title：Journal of clinical microbiology

authors： Garcia LS,Shimizu RY,Bernard CN

更新日期：2000-09-01 00:00:00

abstract：:A rapid colorimetric method for the identification of pathogenic Neisseria (Identicult-Neisseria; Scott Laboratories, Inc.) based on beta-galactosidase, gamma-glutamylaminopeptidase, and gamma-prolylaminopeptidase is described. All 82 clinical isolates of Neisseria gonorrhoeae, 9 clinical isolates of N. meningitidis, ...

journal_title：Journal of clinical microbiology

authors： Hosmer ME,Cohenford MA,Ellner PD

更新日期：1986-07-01 00:00:00