Progression toward an improved DNA amplification-based typing technique in the study of Mycobacterium tuberculosis epidemiology.

abstract：:The diagnosis of brucellosis in cattle and small ruminants requires the use of more than one serological test. The complement fixation test (CFT), the rose bengal test (RBT), and the serum agglutination test (SAT) are among the most useful tests for routine diagnosis. The microagglutination test (MAT) was developed as...

journal_title：Journal of clinical microbiology

authors： Baum M,Zamir O,Bergman-Rios R,Katz E,Beider Z,Cohen A,Banai M

更新日期：1995-08-01 00:00:00

abstract：:A TaqMan real-time PCR assay, the COBAS TaqMan human immunodeficiency virus (HIV) (HPS-CTMHIV) PCR assay, recently developed for the quantification of HIV type 1 RNA in plasma, was evaluated in comparison with the licensed COBAS AMPLICOR HIV-1 MONITOR (CAHIM) assay. In this study, we have analyzed the tests' sensitivi...

journal_title：Journal of clinical microbiology

authors： Perrin L,Pawlotsky JM,Bouvier-Alias M,Sarrazin C,Zeuzem S,Colucci G

更新日期：2006-12-01 00:00:00

abstract：:The clinical, histological, and bacteriological findings in five cases of acute appendicitis caused by Haemophilus segnis are reported. This is the first documentation of appendicitis associated with this organism. ...

journal_title：Journal of clinical microbiology

authors： Welch WD,Southern PM Jr,Schneider NR

更新日期：1986-11-01 00:00:00

abstract：:Although the sensitivity and specificity of nucleic acid amplification assays are high with smear-positive samples, the sensitivity with smear-negative and extrapulmonary samples for the diagnosis of tuberculosis in suspicious tuberculosis cases still remains to be investigated. This study evaluates the performance of...

journal_title：Journal of clinical microbiology

authors： Bicmen C,Gunduz AT,Coskun M,Senol G,Cirak AK,Ozsoz A

更新日期：2011-08-01 00:00:00

abstract：:We conducted a molecular epidemiology study on 120 Mycobacterium tuberculosis isolates from patients presenting pulmonary tuberculosis (TB) in Burkina Faso. Classical antibiogram studies and genetic characterization, using mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing and ...

journal_title：Journal of clinical microbiology

authors： Godreuil S,Torrea G,Terru D,Chevenet F,Diagbouga S,Supply P,Van de Perre P,Carriere C,Bañuls AL

更新日期：2007-03-01 00:00:00

abstract：:Analysis of the electrophoretic profiles of the outer membrane proteins could be used to differentiate Campylobacter jejuni (16 strains) from Campylobacter coli (10 strains). This observation was confirmed by the study of DNA homology obtained by a quantitative filter hybridization method. The hippurate hydrolysis tes...

journal_title：Journal of clinical microbiology

authors： Derclaye I,Delor I,Van Bouchaute M,Moureau P,Wauters G,Cornelis GR

更新日期：1989-05-01 00:00:00

abstract：:The epidemiological implications of the recent separation of "Entamoeba histolytica" into two separate species, pathogenic E. histolytica sensu stricto and commensal E. dispar, will not become apparent without methods of distinguishing between them which are applicable to large numbers of specimens. We have modified a...

journal_title：Journal of clinical microbiology

authors： Britten D,Wilson SM,McNerney R,Moody AH,Chiodini PL,Ackers JP

更新日期：1997-05-01 00:00:00

abstract：:Acinetobacter isolates associated with casualties from the Iraq conflict from the United States were compared with those from the United Kingdom by pulsed-field gel electrophoresis and integron analysis. Representatives of the main outbreak strain associated with casualties from both countries were indistinguishable i...

journal_title：Journal of clinical microbiology

authors： Turton JF,Kaufmann ME,Gill MJ,Pike R,Scott PT,Fishbain J,Craft D,Deye G,Riddell S,Lindler LE,Pitt TL

更新日期：2006-07-01 00:00:00

abstract：:Serological tests to detect Trypanosoma cruzi antibodies have been used for screening blood donors, for epidemic studies, and for diagnosis of probably infected persons. Among different tests, the enzyme-linked immunosorbent assay (ELISA) with total, semipurified, or synthetic antigens has been widely used, mainly due...

journal_title：Journal of clinical microbiology

authors： Ferreira AW,Belem ZR,Lemos EA,Reed SG,Campos-Neto A

更新日期：2001-12-01 00:00:00

abstract：:We report the development of a real-time PCR assay for the quantitative detection of Neospora caninum in infected host tissues. The assay uses the double-stranded DNA-binding dye SYBR Green I to continuously monitor product formation. Oligonucleotide primers were designed to amplify a 76-bp DNA fragment corresponding ...

journal_title：Journal of clinical microbiology

authors： Collantes-Fernández E,Zaballos A,Alvarez-García G,Ortega-Mora LM

更新日期：2002-04-01 00:00:00

abstract：:Helicobacter pylori cagA and vacA genotypes have been used for almost a decade as stable entities to link the severity of gastritis and ulcer disease. We describe here microevolution of the two genomic islands, cag pathogenicity island (cagPAI; 40 kb) and tfs3 (16 kb) from isolates obtained at inclusion (one subclone)...

journal_title：Journal of clinical microbiology

authors： Alvi A,Devi SM,Ahmed I,Hussain MA,Rizwan M,Lamouliatte H,Mégraud F,Ahmed N

更新日期：2007-12-01 00:00:00

abstract：:The number of reports concerning Escherichia coli clinical isolates that produce glutathione S-transferases responsible for fosfomycin resistance (FR-GSTs) has been increasing. We have developed a disk-based potentiation test in which FR-GST producers expand the growth inhibition zone around a Kirby-Bauer disk contain...

journal_title：Journal of clinical microbiology

authors： Nakamura G,Wachino J,Sato N,Kimura K,Yamada K,Jin W,Shibayama K,Yagi T,Kawamura K,Arakawa Y

更新日期：2014-09-01 00:00:00

abstract：:Feces and serum specimens were collected from three farms in Michigan on which approximately 50-lb (8- to 9-week-old) pigs experienced diarrhea just after placement into all-in-all-out finishing barns. The clinical signs (profuse watery diarrhea lasting about 2 weeks and no vomiting) were similar on all farms, and the...

journal_title：Journal of clinical microbiology

authors： Kim Y,Chang KO,Straw B,Saif LJ

更新日期：1999-05-01 00:00:00

abstract：:The extent of genetic and antigenic variation found in a population of human parainfluenza virus type 1 (HPIV-1) during a single local epidemic was investigated. Fifteen HPIV-1 strains isolated from children in 1991 were analyzed. Nucleotide sequence variation in the hemagglutinin-neuraminidase protein (HN) gene demon...

journal_title：Journal of clinical microbiology

authors： Henrickson KJ,Savatski LL

更新日期：1996-03-01 00:00:00

abstract：:The modification of an auxanographic method used in yeast species identification to determine potassium nitrate (KNO3) assimilation resulted in a simple and inexpensive KNO3 assimilation test medium. The medium provided accurate and reliable results in less than 24 h. ...

journal_title：Journal of clinical microbiology

authors： Pincus DH,Salkin IF,Hurd NJ,Levy IL,Kemna MA

更新日期：1988-02-01 00:00:00

abstract：:Several studies have identified thymidine excess in susceptibility test media as the cause of spurious resistance of various bacteria to sulfamethoxazole-trimethoprim. We document the phenomenon in Salmonella typhi and Salmonella paratyphi-A and demonstrate its occurrence in 3 of 17 (18%) lots of Mueller-Hinton agars ...

journal_title：Journal of clinical microbiology

authors： Escamilla J,Kilpatrick ME,Ugarte HF

更新日期：1986-01-01 00:00:00

abstract：:Hepatitis C virus (HCV) genotype 4 (HCV-4) infection is considered to be difficult to treat and has become increasingly prevalent in European countries, including The Netherlands. Using a molecular epidemiological approach, the present study investigates the genetic diversity and evolutionary origin of HCV-4 in Amster...

journal_title：Journal of clinical microbiology

authors： de Bruijne J,Schinkel J,Prins M,Koekkoek SM,Aronson SJ,van Ballegooijen MW,Reesink HW,Molenkamp R,van de Laar TJ

更新日期：2009-12-01 00:00:00

abstract：:Pluronic polyols markedly improved the growth of two human lymphocyte cell lines when added to the growth medium in concentrations of 0.05 to 0.1%. The results of the current studies suggest that, in addition to the protective effect of polyols against mechanical damage of mammalian cells in submerged cultures, the pl...

journal_title：Journal of clinical microbiology

authors： Mizrahi A

更新日期：1975-07-01 00:00:00

abstract：:Three hundred ninety single sera from adults in Japan, Indonesia, Singapore, and Papua New Guinea were tested for antibodies to human calicivirus (HCV) by a radioimmunoassay blocking test. A high prevalence of antibodies was observed in samples collected in Japan and Southeast Asia. Of 240 serum specimens collected fr...

journal_title：Journal of clinical microbiology

authors： Nakata S,Chiba S,Terashima H,Nakao T

更新日期：1985-10-01 00:00:00

abstract：:Helicobacter pylori strains display remarkable genetic diversity, and the presence of strains bearing the toxigenic vacA s1 allele, a complete cag pathogenicity island (PAI), cagA alleles containing multiple EPIYA phosphorylation sites, and expressing the BabA adhesin correlates with development of gastroduodenal dise...

journal_title：Journal of clinical microbiology

authors： Talarico S,Gold BD,Fero J,Thompson DT,Guarner J,Czinn S,Salama NR

更新日期：2009-06-01 00:00:00

abstract：:To evaluate the risk of a nosocomial spread of Pneumocystis carinii f. sp. hominis (P. carinii hominis), air filter samples from rooms of P. carinii pneumonia (PCP) patients, adjacent corridors, and other hospital environments have been investigated for the presence of P. carinii hominis. Amplified DNA from air filter...

journal_title：Journal of clinical microbiology

authors： Olsson M,Lidman C,Latouche S,Björkman A,Roux P,Linder E,Wahlgren M

更新日期：1998-06-01 00:00:00

abstract：:A rapid phenotypic screening method for herpes simplex virus (HSV) and varicella-zoster virus (VZV) thymidine kinase (TK) genes was developed for monitoring acyclovir-resistant viruses. This method determines the biochemical phenotype of the TK polypeptide, which is synthesized in vitro from viral DNA using a procedur...

journal_title：Journal of clinical microbiology

authors： Suzutani T,Saijo M,Nagamine M,Ogasawara M,Azuma M

更新日期：2000-05-01 00:00:00

abstract：:We examined clinical (pulmonary cryptococcosis and cryptococcal meningitis) and environmental (pigeon excreta) isolates of Cryptococcus neoformans var. neoformans (serotype A) in the southern Japanese prefecture of Nagasaki. The random amplified polymorphic DNA profiles obtained by using three primers revealed six pat...

journal_title：Journal of clinical microbiology

authors： Yamamoto Y,Kohno S,Koga H,Kakeya H,Tomono K,Kaku M,Yamazaki T,Arisawa M,Hara K

更新日期：1995-12-01 00:00:00

abstract：:A biotype analysis was performed on serologically nontypable Haemophilus influenzae isolates from the middle ear effusions and nasopharynges of 33 children with chronic otitis media with effusion (serous otitis media). Over 50% of the H. influenzae isolates from the middle ears belonged to biotype II. The incidence of...

journal_title：Journal of clinical microbiology

authors： DeMaria TF,Lim DJ,Barnishan J,Ayers LW,Birck HG

更新日期：1984-12-01 00:00:00

abstract：:Recent evidence supports a causal relationship between Bartonella (Rochalimaea) henselae, cat-scratch disease (CSD), and bacillary angiomatosis. Cats appear to be the primary reservoir. Blood from 19 cats owned by 14 patients diagnosed with CSD was cultured. Blood samples from cats owned by veterinary students (n = 25...

journal_title：Journal of clinical microbiology

authors： Kordick DL,Wilson KH,Sexton DJ,Hadfield TL,Berkhoff HA,Breitschwerdt EB

更新日期：1995-12-01 00:00:00

abstract：:Pneumocystis jirovecii pneumonia (PcP) is a major cause of respiratory illness in patients with AIDS. The identification of multiple single-nucleotide polymorphisms (SNPs) at three distinct P. jirovecii loci encoding dihydrofolate reductase (DHFR), mitochondrial large-subunit rRNA (mtLSU rRNA), and superoxide dismutas...

journal_title：Journal of clinical microbiology

authors： Esteves F,Gaspar J,De Sousa B,Antunes F,Mansinho K,Matos O

更新日期：2011-05-01 00:00:00

abstract：:We first noted the appearance of thymidine-requiring, gram-negative bacilli in clinical specimens 2 years ago. Since then we have seen 10 patients colonized or infected with these organisms. These strains do not grow on Mueller-Hinton media, growth on MacConkey agar is variable, and growth in API 20E (Analytab Product...

journal_title：Journal of clinical microbiology

authors： King CH,Shlaes DM,Dul MJ

更新日期：1983-07-01 00:00:00

abstract：:In a clinical trial, Strep-A-Chek (a 10-min chromogenic test) was compared with the bacitracin disk susceptibility test for accuracy and turnaround time in the presumptive identification of Streptococcus pyogenes. Among 461 isolates of beta-hemolytic streptococci (344 throat isolates and 117 isolates from other sites)...

journal_title：Journal of clinical microbiology

authors： Yajko DM,Lawrence J,Nassos P,Young J,Hadley WK

更新日期：1986-09-01 00:00:00

abstract：:Bacillus cereus is a well-known cause of food-borne illness, but infection with this organism is not commonly reported because of its usually mild symptoms. A fatal case due to liver failure after the consumption of pasta salad is described and demonstrates the possible severity of the emetic syndrome. ...

journal_title：Journal of clinical microbiology

authors： Dierick K,Van Coillie E,Swiecicka I,Meyfroidt G,Devlieger H,Meulemans A,Hoedemaekers G,Fourie L,Heyndrickx M,Mahillon J

更新日期：2005-08-01 00:00:00

abstract：:Rapid and reliable detection of methicillin-resistant Staphylococcus aureus (MRSA) carriers is crucial for the effective control of MRSA transmission in healthcare facilities. The aim of this study was to verify the performance of the IDI-MRSA real-time PCR assay for direct MRSA detection in diverse mucocutaneous swab...

journal_title：Journal of clinical microbiology

authors： de San N,Denis O,Gasasira MF,De Mendonça R,Nonhoff C,Struelens MJ

更新日期：2007-04-01 00:00:00