Identification of Pneumocystis carinii f. sp. hominis gene sequences in filtered air in hospital environments.

Abstract:

:To evaluate the risk of a nosocomial spread of Pneumocystis carinii f. sp. hominis (P. carinii hominis), air filter samples from rooms of P. carinii pneumonia (PCP) patients, adjacent corridors, and other hospital environments have been investigated for the presence of P. carinii hominis. Amplified DNA from air filters and sputum or bronchoalveolar lavage samples from the PCP patients have been genotyped with the P. carinii hominis genes of the mitochondrial large-subunit (mtLSU) rRNA and the internal transcribed spacers (ITS1 and ITS2) of the rRNA. Genotypes of the two loci were identified by direct sequencing, and for site 85 of the mtLSU locus, three allele-specific PCR assays were used. P. carinii hominis DNA was identified in the air of five of seven PCP patient rooms and in the air of two of four air filtrations from the ward corridors. The P. carinii hominis genotypes were the same in four of the five room air samples as those in the corresponding patients, suggesting a risk of person-to-person transmission of P. carinii hominis from PCP patients. Three of 16 air samples collected in infectious disease wards without the presence of PCP patients and one sample from a cardiology unit in a separate hospital building were also positive, which further strengthens the possibility of acquisition of P. carinii hominis from the environment.

journal_name

J Clin Microbiol

authors

Olsson M,Lidman C,Latouche S,Björkman A,Roux P,Linder E,Wahlgren M

doi

10.1128/JCM.36.6.1737-1740.1998

subject

Has Abstract

pub_date

1998-06-01 00:00:00

pages

1737-40

issue

6

eissn

0095-1137

issn

1098-660X

journal_volume

36

pub_type

杂志文章
  • Prevalence of Campylobacter fetus subsp. jejuni in healthy populations in southern India.

    abstract::Campylobacter fetus subsp. jejuni was isolated from the feces of 14.8% of a random sample of the healthy population of rural southern India. The rate of isolation was highest in preschool children. This finding emphasizes the need to identify markers of pathogenicity in strains of C. fetus subsp. jejuni, which so far ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.15.5.749-751.1982

    authors: Rajan DP,Mathan VI

    更新日期:1982-05-01 00:00:00

  • Simplified bioassay method for measurement of flucytosine or ketoconazole.

    abstract::A simple agar-well diffusion bioassay suitable for measurement of flucytosine or ketoconazole was developed by using Candida pseudotropicalis ATCC 46764 as the assay organism. A test medium composed of (per liter) 7 g of Trypticase peptone, 7 g of YNB (yeast-nitrogen base), 15 g of glucose, and 15 g of agar was seeded...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.22.2.157-160.1985

    authors: Bodet CA 3rd,Jorgensen JH,Drutz DJ

    更新日期:1985-08-01 00:00:00

  • Measurement of active constitutive beta-D-glucosidase (esculinase) in the presence of sodium desoxycholate.

    abstract::The hydrolysis of esculin in the presence of bile has been utilized for many years for the identification of bacteria. It is especially useful in differentiating species of the genus Streptococcus. The procedure is a two-step one. First, the bacterium must grow in a particular concentration of bile, and second, it mus...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.21.3.363-365.1985

    authors: Edberg SC,Trepeta RW,Kontnick CM,Torres AR

    更新日期:1985-03-01 00:00:00

  • Detection of replication-competent human immunodeficiency virus type 1 (HIV-1) in cultures from patients with levels of HIV-1 RNA in plasma suppressed to less than 500 or 50 copies per milliliter.

    abstract::We determined the frequency with which human immunodeficiency virus (HIV) peripheral blood mononuclear cell cultures convert from positive to negative in subjects enrolled in a substudy of AIDS Clinical Trials Group (ACTG) 320, which compared the efficacy of treatment with a combination of indinavir, zidovudine, and l...

    journal_title:Journal of clinical microbiology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1128/jcm.40.6.2089-2094.2002

    authors: Demeter LM,Bosch RJ,Coombs RW,Fiscus S,Bremer J,Johnson VA,Erice A,Jackson JB,Spector SA,Squires KM,Fischl MA,Hughes MD,Hammer SM

    更新日期:2002-06-01 00:00:00

  • Evaluation of Ebola virus inactivation procedures for Plasmodium falciparum malaria diagnostics.

    abstract::Plasmodium falciparum malaria is highly endemic in the three most affected countries in the current epidemic of Ebola virus disease (EVD) in West Africa. As EVD and malaria are clinically indistinguishable, both remain part of the differential diagnosis of ill travelers from returning from areas of EVD transmission. W...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00165-15

    authors: Lau R,Wang A,Chong-Kit A,Ralevski F,Boggild AK

    更新日期:2015-04-01 00:00:00

  • Tyrosine phosphorylation of CagA from Chinese Helicobacter pylori isolates in AGS gastric epithelial cells.

    abstract::Helicobacter pylori strains possessing the cag pathogenicity island (PaI) are associated with the development of gastroduodenal diseases, including gastric cancer. cag PaI products induce the secretion of interleukin-8 (IL-8) from epithelial cells and facilitate the translocation of CagA into the cell cytosol. In East...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.2.786-790.2005

    authors: Zhang Y,Argent RH,Letley DP,Thomas RJ,Atherton JC

    更新日期:2005-02-01 00:00:00

  • Performance assessment of new multiplex probe assay for identification of mycobacteria.

    abstract::A new DNA probe assay (INNO LiPA Mycobacteria; Innogenetics, Ghent, Belgium) for the simultaneous identification, by means of reverse hybridization and line-probe technology, of Mycobacterium tuberculosis complex, Mycobacterium kansasii, Mycobacterium xenopi, Mycobacterium gordonae, the species of the Mycobacterium av...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.39.3.1079-1084.2001

    authors: Tortoli E,Nanetti A,Piersimoni C,Cichero P,Farina C,Mucignat G,Scarparo C,Bartolini L,Valentini R,Nista D,Gesu G,Tosi CP,Crovatto M,Brusarosco G

    更新日期:2001-03-01 00:00:00

  • Bartholin's gland abscess caused by Brucella melitensis.

    abstract::We report herein a case of Bartholin's gland abscess caused by Brucella melitensis. Clinical microbiology laboratory workers in areas where this disease is endemic should be familiar with the bacteriological features of this organism and consider the possibility of a brucellar etiology in a broad range of clinical set...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.42.2.917-918.2004

    authors: Peled N,David Y,Yagupsky P

    更新日期:2004-02-01 00:00:00

  • 30 years of campylobacters: biochemical characteristics and a biotyping proposal for Campylobacter jejuni.

    abstract::Several biochemical test systems were studied for their potential usefulness for the examination of strains of Campylobacter species. Most (81%) of the C. jejuni strains hydrolyzed sodium hippurate, but strains of C. fetus, C. sputorum, and C. fecalis did not. Some (46%) of the C. jejuni strains and all of the C. sput...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.15.6.1065-1073.1982

    authors: Hébert GA,Hollis DG,Weaver RE,Lambert MA,Blaser MJ,Moss CW

    更新日期:1982-06-01 00:00:00

  • Genetic diversity of intimin genes of attaching and effacing Escherichia coli strains.

    abstract::In this study, we determined the sequences of four intimin variant genes detected in attaching and effacing Escherichia coli isolates of human origin. Three of them were novel and were designated eae-eta (eta), eae-iota (iota), and eae-kappa (kappa). The fourth was identical to the recently described eae-zeta (zeta), ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.40.12.4486-4492.2002

    authors: Zhang WL,Köhler B,Oswald E,Beutin L,Karch H,Morabito S,Caprioli A,Suerbaum S,Schmidt H

    更新日期:2002-12-01 00:00:00

  • Encephalitis caused by Chikungunya virus in a traveler from the Kingdom of Tonga.

    abstract::Febrile travelers from countries with unique endemic pathogens pose a significant diagnostic challenge. In this report, we describe the case of a Tongan man presenting with fever, rash, and altered mental status. The diagnosis of Chikungunya encephalitis was made using a laboratory-developed real-time RT-PCR and serol...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01288-14

    authors: Nelson J,Waggoner JJ,Sahoo MK,Grant PM,Pinsky BA

    更新日期:2014-09-01 00:00:00

  • Cloning and sequence of a region of Vibrio cholerae O139 Bengal and its use in PCR-based detection.

    abstract::We isolated and characterized a Vibrio cholerae O139 Bengal-specific DNA region by arbitrary PCR. The fragment contains open reading frames encoding two potential glycosyltransferases possibly involved in capsular polysaccharide or lipopolysaccharide biosynthesis. In order to evaluate the possibility that this region ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.34.12.2904-2908.1996

    authors: Falklind S,Stark M,Albert MJ,Uhlén M,Lundeberg J,Weintraub A

    更新日期:1996-12-01 00:00:00

  • Characterization of Metarhizium viride Mycosis in Veiled Chameleons (Chamaeleo calyptratus), Panther Chameleons (Furcifer pardalis), and Inland Bearded Dragons (Pogona vitticeps).

    abstract::Metarhizium viride has been associated with fatal systemic mycoses in chameleons, but subsequent data on mycoses caused by this fungus in reptiles are lacking. The aim of this investigation was therefore to obtain information on the presence of M. viride in reptiles kept as pets in captivity and its association with c...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02206-16

    authors: Schmidt V,Klasen L,Schneider J,Hübel J,Pees M

    更新日期:2017-03-01 00:00:00

  • Vancomycin-resistant Enterococcus faecium colonization in children.

    abstract::Nosocomial vancomycin-resistant Enterococcus (VRE) infections have been described in only small numbers of pediatric patients. In none of these studies were multivariate analyses performed to assess which factors were independent risk factors in these patients. In the present cohort study of patients admitted to our h...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.2.413-416.1999

    authors: Singh-Naz N,Sleemi A,Pikis A,Patel KM,Campos JM

    更新日期:1999-02-01 00:00:00

  • Early identification of seronegative human immunodeficiency virus type 1 infection with severe presentation.

    abstract::Specific antibodies against human immunodeficiency virus (HIV), usually used for diagnosis, almost invariably become detectable within 3 months of exposure. We report on a patient whose HIV infection was identified early by a combined antigen/antibody test, but seroconversion did not occur for 7 months, until the impl...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00166-07

    authors: Chin BS,Lee SH,Kim GJ,Kee MK,Suh SD,Kim SS

    更新日期:2007-05-01 00:00:00

  • Comparative evaluation of the Iatron serological Candida check kit and the API 20C kit for identification of medically important Candida species.

    abstract::A newly developed commercial serological test (Iatron Laboratories, Inc., Tokyo, Japan) for the rapid identification of medically important species of Candida was evaluated against the API 20C (Analytab Products, Plainview, N.Y.) and the standard Wickerham assimilation and fermentation procedures. Our results indicate...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.13.3.513-518.1981

    authors: Shinoda T,Kaufman L,Padhye AA

    更新日期:1981-03-01 00:00:00

  • Double-label fluorescence immunoassay of bacteria.

    abstract::Fluorescence of sensitized bacterial suspensions, reactive with either fluorescein-labeled or rhodamine-labeled antiglobulins, could be quantitatively distinguished in dual-labeled preparations by fluorescence immunoassay. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: Gillis TP,Thompson JJ

    更新日期:1978-09-01 00:00:00

  • New blood culture medium.

    abstract::A growth medium with a specific oxidation-reduction potential containing peptone, dextrose, sodium succinate, sodium lactate, gelatin, sodium bicarbonate and blue tetrazolium, an indicator dye, in a tris(hydroxymethyl)aminomethane buffer was used to detect the presence of microorganisms in blood. The procedure involve...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: Shih CN,Balish E

    更新日期:1977-09-01 00:00:00

  • Identification of some charcoal-black-pigmented CDC fermentative coryneform group 4 isolates as Rothia dentocariosa and some as Corynebacterium aurimucosum: proposal of Rothia dentocariosa emend. Georg and Brown 1967, Corynebacterium aurimucosum emend. Ya

    abstract::Sixty-three clinical isolates of charcoal-black-pigmented, gram-positive coryneform rods were received for identification by the Centers for Disease Control and Prevention (CDC) and were provisionally designated CDC fermentative coryneform group 4 (FCG4). Forty-five of these were characterized by morphological, physio...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.42.9.4189-4198.2004

    authors: Daneshvar MI,Hollis DG,Weyant RS,Jordan JG,MacGregor JP,Morey RE,Whitney AM,Brenner DJ,Steigerwalt AG,Helsel LO,Raney PM,Patel JB,Levett PN,Brown JM

    更新日期:2004-09-01 00:00:00

  • PCR detection of North American and Central African isolates of epizootic hemorrhagic disease virus (EHDV) based on genome segment 10 of EHDV serotype 1.

    abstract::PCR amplification technology for the detection of epizootic hemorrhagic disease virus (EHDV) ribonucleic acid in cell culture and clinical specimens was developed. With oligoribonucleotide primers selected from genome segment 10 of EHDV serotype 1 (EHDV-1), which codes for two nonstructural proteins (NS3 and NS3a), th...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.36.9.2604-2608.1998

    authors: Aradaib IE,Wilson WC,Schore CE,Mohammed ME,Yilma TD,Cullor JS,Osburn BI

    更新日期:1998-09-01 00:00:00

  • Comparison of DNA fingerprints and somatic serotypes of serogroup B and E Pasteurella multocida isolates.

    abstract::The DNA fingerprint profiles and somatic serotypes of 71 Pasteurella multocida capsule serogroup B isolates, 13 capsule serogroup E isolates, and 16 somatic reference serotype strains were compared. Each of the 16 reference somatic serotypes had a unique DNA fingerprint profile with the HhaI restriction endonuclease. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.6.1518-1524.1992

    authors: Wilson MA,Rimler RB,Hoffman LJ

    更新日期:1992-06-01 00:00:00

  • Direct reverse transcriptase PCR to determine virulence potential of influenza A viruses in birds.

    abstract::A reverse transcriptase PCR (RT-PCR) was used for rapid determination of the hemagglutinin (HA) cleavage site sequence, a marker for the virulence potential of avian influenza viruses. When applied to specimens from chickens experimentally infected with either a virulent or an avirulent virus, RT-PCR uniformly detecte...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.3.748-751.1995

    authors: Horimoto T,Kawaoka Y

    更新日期:1995-03-01 00:00:00

  • Whole-Genome Multilocus Sequence Typing of Extended-Spectrum-Beta-Lactamase-Producing Enterobacteriaceae.

    abstract::Molecular typing has become indispensable in the detection of nosocomial transmission of bacterial pathogens and the identification of sources and routes of transmission in outbreak settings, but current methods are labor-intensive, are difficult to standardize, or have limited resolution. Whole-genome multilocus sequ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01648-16

    authors: Kluytmans-van den Bergh MF,Rossen JW,Bruijning-Verhagen PC,Bonten MJ,Friedrich AW,Vandenbroucke-Grauls CM,Willems RJ,Kluytmans JA

    更新日期:2016-12-01 00:00:00

  • Comparison of a latex agglutination assay and an enzyme-linked immunosorbent assay for detecting cholera toxin.

    abstract::To determine the pandemic potential of Vibrio cholerae, one must demonstrate both the presence of O1 antigen and the production of enterotoxin (CT). Tissue culture or enzyme-linked immunosorbent assays (ELISAs) for CT have been limited to research and reference laboratories. A kit for detecting CT by reversed passive ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.1.128-130.1990

    authors: Almeida RJ,Hickman-Brenner FW,Sowers EG,Puhr ND,Farmer JJ 3rd,Wachsmuth IK

    更新日期:1990-01-01 00:00:00

  • Efficacy of various cell culture procedures for detection of Chlamydia trachomatis and applicability to diagnosis of pediatric infections.

    abstract::In this study, 5-iodo-2'-deoxyuridine-, cytochalasin B-, and cycloheximide-treated and untreated McCoy cells were inoculated with representative strains of Chlamydia trachomatis serotypes, as well as with clinical specimens from a pediatric population, and the quality and quantity of the cytoplasmic inclusions were ex...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.13.4.705-708.1981

    authors: La Scolea LJ Jr,Keddell JE

    更新日期:1981-04-01 00:00:00

  • Analysis of culture-dependent versus culture-independent techniques for identification of bacteria in clinically obtained bronchoalveolar lavage fluid.

    abstract::The diagnosis and management of pneumonia are limited by the use of culture-based techniques of microbial identification, which may fail to identify unculturable, fastidious, and metabolically active viable but unculturable bacteria. Novel high-throughput culture-independent techniques hold promise but have not been s...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01028-14

    authors: Dickson RP,Erb-Downward JR,Prescott HC,Martinez FJ,Curtis JL,Lama VN,Huffnagle GB

    更新日期:2014-10-01 00:00:00

  • Evaluation of recombinant antigens for serodiagnosis of Chagas' disease in South and Central America.

    abstract::The commercially available diagnostic tests for Chagas' disease employ whole extracts or semipurified fractions of Trypanosoma cruzi epimastigotes. Considerable variation in the reproducibility and reliability of these tests has been reported by different research laboratories, mainly due to cross-reactivity with othe...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.5.1554-1560.1999

    authors: Umezawa ES,Bastos SF,Camargo ME,Yamauchi LM,Santos MR,Gonzalez A,Zingales B,Levin MJ,Sousa O,Rangel-Aldao R,da Silveira JF

    更新日期:1999-05-01 00:00:00

  • Use of polymerase chain reaction for detection of toxigenic Vibrio cholerae O1 strains from the Latin American cholera epidemic.

    abstract::In January 1991, an outbreak of cholera started in Peru and spread throughout most of Latin America within 8 months. As of March 1992, over 450,000 cases and approximately 4,000 deaths have been reported to the Pan American Health Organization. The causative organism is toxigenic Vibrio cholerae O1 of the El Tor bioty...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.8.2118-2121.1992

    authors: Fields PI,Popovic T,Wachsmuth K,Olsvik O

    更新日期:1992-08-01 00:00:00

  • Immunoglobulin M capture immunoassay in investigation of coxsackievirus B5 and B6 outbreaks in South Australia.

    abstract::An immunoglobulin M (IgM) capture enzyme immunoassay was used to detect major overlapping outbreaks of disease in South Australia caused by coxsackieviruses B5 (CBV-5) and B6 (CBV-6). CBV-5-specific IgM was detected in patients presenting in spring 1992 with acute febrile illnesses, rash, severe acute respiratory dise...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.6.1628-1631.1995

    authors: Goldwater PN

    更新日期:1995-06-01 00:00:00

  • Evaluation of Vitek MS for Differentiation of Cryptococcus neoformans and Cryptococcus gattii Genotypes.

    abstract::Cryptococcus neoformans and Cryptococcus gattii are the main pathogenic species of invasive cryptococcosis among the Cryptococcus species. Taxonomic studies have shown that these two taxa have different genotypes or molecular types with biological and ecoepidemiological peculiarities. Matrix-assisted laser desorption ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01282-18

    authors: Siqueira LPM,Gimenes VMF,de Freitas RS,Melhem MSC,Bonfietti LX,da Silva AR Jr,Souza Santos LB,Motta AL,Rossi F,Benard G,de Almeida JN Jr

    更新日期:2019-01-02 00:00:00