Cloning and sequence of a region of Vibrio cholerae O139 Bengal and its use in PCR-based detection.

abstract：:Morganella ("Proteus") morganii is the only species in the recently proposed genus Morganella, but we suspect there are yet undescribed species in the genus. Two candidates for new species were recently investigated. Nineteen strains isolated from clinical specimens resembled M. morganii but were lysine positive and n...

journal_title：Journal of clinical microbiology

authors： Hickman FW,Framer JJ 3rd,Steigerwalt AG,Brenner DJ

更新日期：1980-07-01 00:00:00

abstract：:Serotyping of Chlamydia trachomatis strains usually requires three to six serial passages in shell vials to attain sufficient antigen for typing procedures. To circumvent this problem, we developed a rapid low-passage method for serotyping of C. trachomatis clinical isolates. Isolates with an inclusion count of greate...

journal_title：Journal of clinical microbiology

authors： Suchland RJ,Stamm WE

更新日期：1991-07-01 00:00:00

abstract：:During lyssavirus surveillance, 1,221 bats of at least 30 species were collected from 25 locations in Kenya. One isolate of Lagos bat virus (LBV) was obtained from a dead Eidolon helvum fruit bat. The virus was most similar phylogenetically to LBV isolates from Senegal (1985) and from France (imported from Togo or Egy...

journal_title：Journal of clinical microbiology

authors： Kuzmin IV,Niezgoda M,Franka R,Agwanda B,Markotter W,Beagley JC,Urazova OY,Breiman RF,Rupprecht CE

更新日期：2008-04-01 00:00:00

abstract：:A Salmonella enterica serotype Typhi strain was cultured from blood and fecal samples from a 54-year-old man with fever and diarrhea. He had returned from travel to the Philippines a few days earlier. Phenotypic and genotypic analysis confirmed the production of the SHV-12 extended-spectrum beta-lactamase. ...

journal_title：Journal of clinical microbiology

authors： Al Naiemi N,Zwart B,Rijnsburger MC,Roosendaal R,Debets-Ossenkopp YJ,Mulder JA,Fijen CA,Maten W,Vandenbroucke-Grauls CM,Savelkoul PH

更新日期：2008-08-01 00:00:00

abstract：:The frequency of and risk factors for methicillin-resistant Staphylococcus aureus (MRSA) transmission from a MRSA index person to household contacts were assessed in this prospective study. Between January 2005 and December 2007, 62 newly diagnosed MRSA index persons (46 patients and 16 health care workers) and their ...

journal_title：Journal of clinical microbiology

authors： Mollema FP,Richardus JH,Behrendt M,Vaessen N,Lodder W,Hendriks W,Verbrugh HA,Vos MC

更新日期：2010-01-01 00:00:00

abstract：:Nocardia brasiliensis, the second most frequently isolated aerobic actinomycete in the clinical laboratory, is usually associated with localized cutaneous infections. However, 22% of 238 N. brasiliensis isolates from the United States and 12% of 66 isolates from Queensland, Australia, which had been collected over a 1...

journal_title：Journal of clinical microbiology

authors： Wallace RJ Jr,Brown BA,Blacklock Z,Ulrich R,Jost K,Brown JM,McNeil MM,Onyi G,Steingrube VA,Gibson J

更新日期：1995-06-01 00:00:00

abstract：:Skin biopsies of 36 patients with erythema migrans and acrodermatitis chronica atrophicans (ACA) before therapy and those of 8 patients after therapy were examined for Borrelia burgdorferi DNA by PCR. Skin biopsies of 27 patients with dermatological diseases other than Lyme borreliosis and those of 10 healthy persons ...

journal_title：Journal of clinical microbiology

authors： Brettschneider S,Bruckbauer H,Klugbauer N,Hofmann H

更新日期：1998-09-01 00:00:00

abstract：:An immunofluorescence assay (IFA) for the detection of immunoglobulin G antibodies directed against Helicobacter pylori was evaluated by comparing 20 serum specimens from patients with a positive urease test on biopsy material and 20 serum specimens from patients with a negative test and with defined clinical symptoms...

journal_title：Journal of clinical microbiology

authors： Faulde M,Putzker M,Mertes T,Sobe D

更新日期：1991-02-01 00:00:00

abstract：:Two ambient-temperature, dry plasma transport systems, ViveST tubes and RNASound RNA sampling cards, and two extraction methods were compared to frozen plasma for HIV-1 RNA recovery. Significant RNA loss occurred: ViveST+MiniMag > ViveST+QIAamp > RNASound+QIAamp. RNA loss and low specimen volumes may affect the sensit...

journal_title：Journal of clinical microbiology

authors： Levine M,Beck I,Styrchak S,Pepper G,Frenkel L

更新日期：2016-07-01 00:00:00

abstract：:Tropheryma whipplei, the agent of Whipple's disease, is a gram-positive rod-shaped bacterium that belongs to the group of actinobacteria. In order to produce monoclonal antibodies (MAbs) against this bacterium, we inoculated mice with two different strains, Slow2 and Endo5. We produced 13 and 10 MAbs against Slow2 and...

journal_title：Journal of clinical microbiology

authors： Yu Y,Kowalczewska M,Decloquement P,Nappez C,La Scola B

更新日期：2006-11-01 00:00:00

abstract：:To examine the validity of cultures of fluid collected through drainage catheters, we reviewed retrospectively fluid specimens that had been collected through catheters in place for at least 2 days. These specimens were taken from patients at a large tertiary-care hospital. A total of 974 specimens representing 620 pa...

journal_title：Journal of clinical microbiology

authors： Everts RJ,Heneghan JP,Adholla PO,Reller LB

更新日期：2001-01-01 00:00:00

abstract：:Chlamydia trachomatis was recoved from 20% (36/180) of women attending a venereal disease clinic. All infected women had chlamydial antibodies in their serum and cervical secretions. However, the background rates of chlamydial antibody in chlamydia-negative women were very high. Measurement of antibodies in serum (com...

journal_title：Journal of clinical microbiology

authors： Schachter J,Cles L,Ray R,Hines PA

更新日期：1979-11-01 00:00:00

abstract：:Molecular techniques are playing an important role in the diagnosis of nontuberculous mycobacterial infections. This case report describes a chronic soft tissue infection in an immunocompetent patient caused by a previously undescribed pigmented, rapidly growing Mycobacterium species, emphasizing the importance of cli...

journal_title：Journal of clinical microbiology

authors： Turenne CY,Suchak AA,Wolfe JN,Kabani A,Nicolle LE

更新日期：2003-06-01 00:00:00

abstract：:During a 1-year survey of Shiga toxin-producing Escherichia coli (STEC) prevalence in central France, 2,143 samples were investigated by PCR for Shiga toxin-encoding genes. A total of 330 (70%) of 471 fecal samples collected from healthy cattle at the Clermont-Ferrand slaughterhouse, 47 (11%) of 411 beef samples, 60 (...

journal_title：Journal of clinical microbiology

authors： Pradel N,Livrelli V,De Champs C,Palcoux JB,Reynaud A,Scheutz F,Sirot J,Joly B,Forestier C

更新日期：2000-03-01 00:00:00

abstract：:Human infections with Salmonella enterica subspecies enterica serovar Senftenberg are often associated with exposure to poultry flocks, farm environments, or contaminated food. The recent emergence of multidrug-resistant isolates has raised public health concerns. In this study, comparative genomics and phenotypic ana...

journal_title：Journal of clinical microbiology

authors： Abd El Ghany M,Shi X,Li Y,Ansari HR,Hill-Cawthorne GA,Ho YS,Naeem R,Pickard D,Klena JD,Xu X,Pain A,Hu Q

更新日期：2016-08-01 00:00:00

abstract：:An enzyme immunoassay (enzyme-linked immunosorbent assay [ELISA]) to detect hamster antibody to toxins A and B of Clostridium difficile was developed in which toxin preparations are used to coat the solid phase. The specificity of the assay was supported by blocking tests with the toxin preparations and proteins from ...

journal_title：Journal of clinical microbiology

authors： Viscidi RP,Yolken RH,Laughon BE,Bartlett JG

更新日期：1983-08-01 00:00:00

abstract：:A study of 180 healthy cats found that 15.6% were feline leukemia virus (FeLV) positive, 8.3% were feline immunodeficiency virus (FIV) positive, and 1.1% were FIV and FeLV positive, which corresponded to 30.4, 13.8, and 2.6, of 115 cats with FIV- and FeLV-related symptoms, respectively. Differences were seen in the se...

journal_title：Journal of clinical microbiology

authors： Arjona A,Escolar E,Soto I,Barquero N,Martin D,Gomez-Lucia E

更新日期：2000-09-01 00:00:00

abstract：:We have developed a novel and practical DNA-RNA hybridization assay for the detection and identification of Campylobacter pylori in the gastric mucosa. This technique utilizes a [32P]ddATP-labeled synthetic oligonucleotide probe complementary to a nucleotide sequence present in C. pylori 16S rRNA. This probe is very s...

journal_title：Journal of clinical microbiology

authors： Morotomi M,Hoshina S,Green P,Neu HC,LoGerfo P,Watanabe I,Mutai M,Weinstein IB

更新日期：1989-12-01 00:00:00

abstract：:Until recently, the laboratory diagnosis of central nervous system (CNS) infections with herpes simplex virus (HSV) has been limited by poor sensitivity and/or specificity. We assessed the diagnostic utility of PCR for detection of HSV in over 2,100 specimens referred to the Mayo Clinic from August 1993 to May 1996. D...

journal_title：Journal of clinical microbiology

authors： Mitchell PS,Espy MJ,Smith TF,Toal DR,Rys PN,Berbari EF,Osmon DR,Persing DH

更新日期：1997-11-01 00:00:00

abstract：:The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of Coronavirus Disease 2019 (COVID-19). While molecular-based testing is used to diagnose COVID-19, serologic testing of antibodies specific to SARS-CoV-2 is used to detect past infection. While most serologic assays are qualitativ...

journal_title：Journal of clinical microbiology

authors： Higgins V,Fabros A,Kulasingam V

更新日期：2021-01-22 00:00:00

abstract：:Stable dwarf forms of Staphylococcus aureus have been identified in clinical specimens as the sole or predominant isolate in eight cases. These organisms have been shown to be menadione or thiamine dependent, i.e., cultivation in the presence of one of these agents has permitted growth of colonies which appear typical...

journal_title：Journal of clinical microbiology

authors： Acar JF,Goldstein FW,Lagrange P

更新日期：1978-08-01 00:00:00

abstract：:High-risk human papillomavirus (hrHPV) testing is now being introduced as a potential primary screening test for improved detection of cervical precancer and cancer. Current U.S. Food and Drug Administration-approved tests are batch tests that take several hours to complete. A rapid, non-batch test might permit point-...

journal_title：Journal of clinical microbiology

authors： Einstein MH,Smith KM,Davis TE,Schmeler KM,Ferris DG,Savage AH,Gray JE,Stoler MH,Wright TC Jr,Ferenczy A,Castle PE

更新日期：2014-06-01 00:00:00

abstract：:In vitro studies established that neither cloth nor disposable diapers demonstrably contributed to the growth of Escherichia coli, Proteus vulgaris, Staphylococcus aureus, or Candida albicans when urine was present as a growth medium. In a clinical study of 166 children, the microbial skin flora of children with atopi...

journal_title：Journal of clinical microbiology

authors： Keswick BH,Seymour JL,Milligan MC

更新日期：1987-02-01 00:00:00

abstract：:We report the recovery of Histoplasma capsulatum from blood specimens cultured for Mycobacterium sp. in BACTEC 13A radiometric medium. H. capsulatum was recovered from six of eight blood specimens submitted for mycobacterial cultures from five human immunodeficiency virus-positive individuals. Initial positive metabol...

journal_title：Journal of clinical microbiology

authors： Merz WG,Kodsy S,Merz CS

更新日期：1992-01-01 00:00:00

abstract：:An outbreak of coronavirus disease 2019 (COVID-19) caused by a novel coronavirus (severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2]) began in Wuhan, Hubei, China, in December 2019 and spread rapidly worldwide. The response by the Alberta Precision Laboratories, Public Health Laboratory (ProvLab), AB, Canada...

journal_title：Journal of clinical microbiology

authors： Pabbaraju K,Wong AA,Douesnard M,Ma R,Gill K,Dieu P,Fonseca K,Zelyas N,Tipples GA

更新日期：2020-07-23 00:00:00

abstract：:We compared trends of annual resistance rates calculated from results for all isolates and for the first isolate of Staphylococcus aureus, Klebsiella pneumoniae, and Acinetobacter baumannii per patient over a 3-year period from 2001 through 2003. Antimicrobial susceptibility results of inpatients were extracted from a...

journal_title：Journal of clinical microbiology

authors： Lee SO,Cho YK,Kim SY,Lee ES,Park SY,Seo YH

更新日期：2004-10-01 00:00:00

abstract：:We report a case of disseminated Scedosporium/Pseudallescheria infection due to Pseudallescheria boydii sensu stricto after lung transplantation in a patient with cystic fibrosis. Dissemination occurred under voriconazole. Despite surgery and combination therapy with voriconazole, caspofungin, and terbinafine, the pat...

journal_title：Journal of clinical microbiology

authors： Morio F,Horeau-Langlard D,Gay-Andrieu F,Talarmin JP,Haloun A,Treilhaud M,Despins P,Jossic F,Nourry L,Danner-Boucher I,Pattier S,Bouchara JP,Le Pape P,Miegeville M

更新日期：2010-05-01 00:00:00

abstract：:In the last 2 decades, a variety of different molecular typing methods have been developed to differentiate strains of Mycobacterium avium subsp. paratuberculosis. The most successful techniques are based on insertion sequences, repetitive loci, comparative genomics, or single nucleotide polymorphisms. In the present ...

journal_title：Journal of clinical microbiology

authors： Griffiths TA,Rioux K,De Buck J

更新日期：2008-04-01 00:00:00

abstract：:Actinobacillus actinomycetemcomitans is an important pathogen in the etiology of severe periodontitis. For epidemiological studies on the prevalence of certain pathogenic clones and transmission of this bacterium, adequate typing methods are necessary. The purpose of this study was to compare six different typing meth...

journal_title：Journal of clinical microbiology

authors： van Steenbergen TJ,Bosch-Tijhof CJ,van Winkelhoff AJ,Gmür R,de Graaff J

更新日期：1994-11-01 00:00:00

abstract：:The complete nucleotide sequence of one of the immediate-early genes of human herpesvirus 6 variant B was determined and compared with that of variant A reported by Martin et al. (M.D. Martin, J. Nicholas, B. J. Thomson, C. Newman, and R. W. Honess, J. Virol. 65:5381-5390, 1991). While it was reported that two open re...

journal_title：Journal of clinical microbiology

authors： Yamamoto T,Mukai T,Kondo K,Yamanishi K

更新日期：1994-02-01 00:00:00