Abstract:
:To determine the pandemic potential of Vibrio cholerae, one must demonstrate both the presence of O1 antigen and the production of enterotoxin (CT). Tissue culture or enzyme-linked immunosorbent assays (ELISAs) for CT have been limited to research and reference laboratories. A kit for detecting CT by reversed passive latex agglutination is now commercially available and was used to test 168 strains of V. cholerae O1 and non-O1. When compared with the routine ELISA, the latex test was 98% accurate (86 of 88) for serogroup O1 strains and 100% accurate (80 of 80) for non-O1 strains. For both O1 and non-O1 study strains, the sensitivity of the latex agglutination test was 0.97 and the specificity was 1.00 when results were compared with ELISA results. The latex test is commercially available and has the advantages of being less complicated and less time-consuming than the ELISA.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Almeida RJ,Hickman-Brenner FW,Sowers EG,Puhr ND,Farmer JJ 3rd,Wachsmuth IKdoi
10.1128/JCM.28.1.128-130.1990subject
Has Abstractpub_date
1990-01-01 00:00:00pages
128-30issue
1eissn
0095-1137issn
1098-660Xjournal_volume
28pub_type
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.31.3.758-759.1993
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.17.1.16-21.1983
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journal_title:Journal of clinical microbiology
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更新日期:1996-03-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.18.2.242-247.1983
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更新日期:1995-01-01 00:00:00
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:1989-06-01 00:00:00
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