Evaluation of recombinant antigens for serodiagnosis of Chagas' disease in South and Central America.

abstract：:Cryptococcus neoformans var. gattii is a causative agent of cryptococcosis and is thought to have a specific ecological association with a number of Eucalyptus species in Australia. However, the role that the tree plays in the life cycle of the fungus and the nature of the infectious propagule are not well understood....

journal_title：Journal of clinical microbiology

authors： Halliday CL,Carter DA

更新日期：2003-02-01 00:00:00

abstract：:Hepatitis C virus (HCV) infection typically leads to antibody response within weeks after primary infection. Here, we describe the case of a child infected with HCV by mother-to-child transmission who remained persistently seronegative despite the presence of high levels of circulating HCV RNA. ...

journal_title：Journal of clinical microbiology

authors： Larouche A,Gaëtan G,El-Bilali N,Quesnel-Vallières M,Martin SR,Alvarez F,Shoukry NH,Soudeyns H

更新日期：2012-07-01 00:00:00

abstract：:Survival of anaerobic and aerobic bacteria in a commercially available, non-supportive, gassed (oxygen-free) transport container (Anaport) was evaluated quantitatively. Saline-suspended obligate anaerobes survived significantly better in the gassed container in aerobic control tubes (P less than 0.025, t test), and co...

journal_title：Journal of clinical microbiology

authors： Chow AW,Cunningham PJ,Guze LB

更新日期：1976-02-01 00:00:00

abstract：:Acinetobacter spp. are important nosocomial pathogens reported with increasing frequency in outbreaks of cross-infection during the past 2 decades. The majority of such outbreaks are caused by Acinetobacter baumannii. To investigate whether desiccation tolerance may be involved in the ability of certain strains of A. ...

journal_title：Journal of clinical microbiology

authors： Jawad A,Seifert H,Snelling AM,Heritage J,Hawkey PM

更新日期：1998-07-01 00:00:00

abstract：:Shortly after adopting a 6-week-old cat, a veterinarian was bitten on the left index finger. Within 3 weeks, he developed headache, fever, and left axillary lymphadenopathy. Initial blood cultures from the cat and veterinarian were sterile. Repeat cultures from the cat grew Bartonella-like organisms with lophotrichous...

journal_title：Journal of clinical microbiology

authors： Kordick DL,Hilyard EJ,Hadfield TL,Wilson KH,Steigerwalt AG,Brenner DJ,Breitschwerdt EB

更新日期：1997-07-01 00:00:00

abstract：:In a cohort of subjects from Italy, anti-hepatitis C virus (HCV) and HCV RNA [HCV(+) subgroup] prevalences were 24.6 and 79.6%, respectively. HCV types 1b and 2a/c accounted for 95% of infections. Adjusted alanine aminotransferase levels were higher in males than in females and in RNA-positive subjects than in RNA-neg...

journal_title：Journal of clinical microbiology

authors： Osella AR,Sonzogni L,Cavallini A,Foti L,Guerra V,Di Leo A,Mondelli MU,Misciagna G,Silini EM

更新日期：1999-07-01 00:00:00

abstract：:To facilitate the identification and serotyping of Salmonella species, we established a wide variety of murine monoclonal antibodies (MAbs) that were reactive with the lipopolysaccharides (LPSs) of Salmonella serogroups B to E. An effective approach for generating LPS-reactive hybridomas was used; this required immuni...

journal_title：Journal of clinical microbiology

authors： Luk JM,Lindberg AA

更新日期：1991-11-01 00:00:00

abstract：:INTRODUCTIONSyndromic panels were first FDA cleared for detection of respiratory pathogens in 2008. Since then, other panels have been approved by the FDA, and most recently, the FilmArray meningitis/encephalitis panel (BioFire, Salt Lake City, UT) has become available. This assay detects 14 targets within 1 h and inc...

journal_title：Journal of clinical microbiology

authors： Dien Bard J,Alby K

更新日期：2018-03-26 00:00:00

abstract：:Between 1991 and 1995, an apparent high rate of Staphylococcus warneri bacteremias at the Royal Children's Hospital, Melbourne, Victoria, Australia, raised the possibility of a virulent nosocomial strain. In a retrospective review of 30 S. warneri bacteremias in children, organisms were viable and verified in 22 episo...

journal_title：Journal of clinical microbiology

authors： Buttery JP,Easton M,Pearson SR,Hogg GG

更新日期：1997-08-01 00:00:00

abstract：:The disease spectrum associated with human bocavirus-1 infection remains to be fully defined. We report a case of bocavirus-1-associated bronchiolitis, leading to severe respiratory failure and extracorporeal membrane oxygenation in a 4-year-old child, and suggest blood testing for human bocavirus-1 in children with s...

journal_title：Journal of clinical microbiology

authors： Edner N,Castillo-Rodas P,Falk L,Hedman K,Söderlund-Venermo M,Allander T

更新日期：2012-02-01 00:00:00

abstract：:The prevalence of class D extended-spectrum oxacillinases (ES-OXAs) in ceftazidime-resistant strains of Pseudomonas aeruginosa is often underestimated by double-disk synergy tests (DDST) using clavulanate. A DDST with a customized distance between a disk of ceftazidime or cefepime and inhibitors (clavulanate and imipe...

journal_title：Journal of clinical microbiology

authors： Hocquet D,Dehecq B,Bertrand X,Plésiat P

更新日期：2011-06-01 00:00:00

abstract：:An optimized reverse transcription (RT)-PCR protocol for the intravitam detection of rabies virus genomic RNA was tested with clinical samples obtained from 28 patients suspected of having rabies, 9 of whom were confirmed to have had rabies by postmortem examination. RT-PCR using saliva combined with an immunofluoresc...

journal_title：Journal of clinical microbiology

authors： Crepin P,Audry L,Rotivel Y,Gacoin A,Caroff C,Bourhy H

更新日期：1998-04-01 00:00:00

abstract：:The serological properties of six new pneumococcal capsular types are described. A table listing all 90 pneumococcal types and their cross-reactions is included. ...

journal_title：Journal of clinical microbiology

authors： Henrichsen J

更新日期：1995-10-01 00:00:00

abstract：:Infected aneurysms caused by Yersinia are very uncommon and are principally due to Yersinia enterocolitica. We describe the first case of an infected aneurysm caused by Yersinia pseudotuberculosis in an elderly patient with a history of atherosclerotic cardiovascular disease. ...

journal_title：Journal of clinical microbiology

authors： Hadou T,Elfarra M,Alauzet C,Guinet F,Lozniewski A,Lion C

更新日期：2006-09-01 00:00:00

abstract：:An evaluation was undertaken to determine the optimal method for the in vitro susceptibility testing of 26 Nocardia asteroides complex isolates to the following antimicrobial agents: amikacin, ampicillin, amoxicillin-clavulanate, ceftriaxone, ciprofloxacin, erythromycin, imipenem, minocycline, and trimethoprim-sulfame...

journal_title：Journal of clinical microbiology

authors： Ambaye A,Kohner PC,Wollan PC,Roberts KL,Roberts GD,Cockerill FR 3rd

更新日期：1997-04-01 00:00:00

abstract：:We examined several factors for their effects on the serological reactivity of Legionella antigens used for direct or indirect fluorescent-antibody tests. These factors included media, methods of killing, strain differences, and the nature of the reactivity with diverse human sera. The maximum serological reactivities...

journal_title：Journal of clinical microbiology

authors： Benson RF,Malcolm GB,Pine L,Harrell WK

更新日期：1983-05-01 00:00:00

abstract：:We report in this work a highly sensitive and nonradioactive PCR method for the detection of the four species of parasite causing human malaria. Plasmodium-specific primers corresponding to the small-subunit rRNA genes of the malaria parasite were used, and a 291-bp fragment was amplified. Our results showed a high sp...

journal_title：Journal of clinical microbiology

authors： Ciceron L,Jaureguiberry G,Gay F,Danis M

更新日期：1999-01-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry with a Bruker Daltonics microflex LT system was applied to 90 well-characterized catalase-negative, Gram-positive cocci not belonging to the streptococci or enterococci. Biotyper version 2.0.43.1 software was used singly or in com...

journal_title：Journal of clinical microbiology

authors： Christensen JJ,Dargis R,Hammer M,Justesen US,Nielsen XC,Kemp M,Danish MALDI-TOF MS Study Group.

更新日期：2012-05-01 00:00:00

abstract：:Molecular techniques are playing an important role in the diagnosis of nontuberculous mycobacterial infections. This case report describes a chronic soft tissue infection in an immunocompetent patient caused by a previously undescribed pigmented, rapidly growing Mycobacterium species, emphasizing the importance of cli...

journal_title：Journal of clinical microbiology

authors： Turenne CY,Suchak AA,Wolfe JN,Kabani A,Nicolle LE

更新日期：2003-06-01 00:00:00

abstract：:The NCCLS proposed standard M38-P describes standard parameters for testing the fungistatic antifungal activities (MICs) of established agents against filamentous fungi (molds); however, standard conditions are not available for testing their fungicidal activities (minimum fungicidal or lethal concentrations [MFCs]). ...

journal_title：Journal of clinical microbiology

authors： Espinel-Ingroff A

更新日期：2001-03-01 00:00:00

abstract：:Herpes simplex virus (HSV) infections are very common in the general population and among immunocompromised patients. Acyclovir (ACV) is an effective treatment which is widely used. We deemed it essential to conduct a wide and coordinated survey of the emergence of ACV-resistant HSV strains. We have formed a network o...

journal_title：Journal of clinical microbiology

authors： Danve-Szatanek C,Aymard M,Thouvenot D,Morfin F,Agius G,Bertin I,Billaudel S,Chanzy B,Coste-Burel M,Finkielsztejn L,Fleury H,Hadou T,Henquell C,Lafeuille H,Lafon ME,Le Faou A,Legrand MC,Maille L,Mengelle C,Morand P,

更新日期：2004-01-01 00:00:00

abstract：:To determine sensitivity and specificity of immunofluorescence assay (IFA) for detection of Helicobacter pylori, we studied 151 patients. Biopsies of gastric mucosae were obtained for culture, histological testing, and IFA. H. pylori serum antibodies were tested by enzyme-linked immunosorbent assay. IFA was done on Fo...

journal_title：Journal of clinical microbiology

authors： Rivera E,López-Vidal Y,Luqueño V,Ruiz-Palacios GM

更新日期：1991-08-01 00:00:00

abstract：:Statistical analysis of a limiting dilution assay (LDA) showed that the occurrence of infectious human immunodeficiency virus type 1 (HIV-1)-harboring cells in serially diluted samples of peripheral blood mononuclear cells (PBMCs) of HIV-1-seropositive patients fits the model describing a single-hit Poisson distributi...

journal_title：Journal of clinical microbiology

authors： Lu W,Manolikakis G,Andrieu JM

更新日期：1992-10-01 00:00:00

abstract：:The accuracy of disk susceptibility tests with ampicillin and ampicillin-sulbactam was not improved when the amount of ampicillin was increased from 10 to 20 or 30 micrograms per disk. For testing members of the family Enterobacteriaceae, ampicillin disk tests correlated better with broth microdilution tests when the ...

journal_title：Journal of clinical microbiology

authors： Barry AL,Jones RN

更新日期：1988-04-01 00:00:00

abstract：:Bordetella pertussis was diagnosed in a human immunodeficiency virus-infected patient by a newly developed method in which bacterial DNA is amplified directly from sputum Gram-stained slides. The validation of the method is described along with an additional new PCR-based assay that can distinguish between B. pertussi...

journal_title：Journal of clinical microbiology

authors： Vielemeyer O,Crouch JY,Edberg SC,Howe JG

更新日期：2004-02-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) for detection of feline leukemia virus (FeLV) p27 in saliva was tested for its accuracy and sensitivity in diagnosing FeLV infections. Saliva and serum samples from 564 clinical cases were tested with a 99.2% specificity. The overall accuracy of the saliva ELISA reactive to...

journal_title：Journal of clinical microbiology

authors： Lewis MG,Wright KA,Lafrado LJ,Shanker PJ,Palumbo NE,Lemoine ED,Olsen RG

更新日期：1987-07-01 00:00:00

abstract：:A procedure for discrimination between scrapie and bovine spongiform encephalopathy (BSE) in sheep is of importance for establishing whether BSE has entered the sheep population. Since BSE has not yet been found in sheep at the farm level, such discrimination procedures can be developed only with experimental sheep BS...

journal_title：Journal of clinical microbiology

authors： Thuring CM,Erkens JH,Jacobs JG,Bossers A,Van Keulen LJ,Garssen GJ,Van Zijderveld FG,Ryder SJ,Groschup MH,Sweeney T,Langeveld JP

更新日期：2004-03-01 00:00:00

abstract：:There are several convenient and accurate molecular assays to detect respiratory bacterial infection. The NeoPlex RB-8 detection kit (NeoPlex RB-8) is a new multiplex real-time PCR assay that simultaneously detects Streptococcus pneumoniae, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, Haemo...

journal_title：Journal of clinical microbiology

authors： Kim JW,Hong SS,Lee IS,Chi HY,Kim SO,Kim HN,Hong SP

更新日期：2020-01-28 00:00:00

abstract：:Public health microbiology laboratories (PHLs) are on the cusp of unprecedented improvements in pathogen identification, antibiotic resistance detection, and outbreak investigation by using whole-genome sequencing (WGS). However, considerable challenges remain due to the lack of common standards. Here, we describe the...

journal_title：Journal of clinical microbiology

authors： Kozyreva VK,Truong CL,Greninger AL,Crandall J,Mukhopadhyay R,Chaturvedi V

更新日期：2017-08-01 00:00:00

abstract：:The human herpesvirus 6 (HHV-6) early protein, p41, encoded by the U27 gene has been detected in oligodendrocytes of multiple sclerosis (MS) patients by using a monoclonal antibody (MAb to p41/38). We here report the antigenic epitope of HHV-6 p41 recognized by this MAb. First, we established that the MAb to p41/38 re...

journal_title：Journal of clinical microbiology

authors： Xu Y,Linde A,Dahl H,Winberg G

更新日期：2001-04-01 00:00:00