Comparison of DNA fingerprints and somatic serotypes of serogroup B and E Pasteurella multocida isolates.

abstract：:The use of a new commercial separation column on whole test serum improved the method for detecting immunoglobulin M antibody to Epstein-Barr virus. The efficiency of this product in absorbing interfering immunoglobulin G was similar to that of another absorption product, staphylococcal protein A, but it has the advan...

journal_title：Journal of clinical microbiology

authors： Sumaya CV,Ench Y,Carrillo MA,Pope RM

更新日期：1984-08-01 00:00:00

abstract：:We attempted to find a relationship between the microbiological properties of bloodstream isolates of methicillin-resistant Staphylococcus aureus (MRSA) and the efficacy of vancomycin in the treatment of bacteremia. Vancomycin susceptibility testing was performed, and bactericidal activity was determined for 30 isolat...

journal_title：Journal of clinical microbiology

authors： Sakoulas G,Moise-Broder PA,Schentag J,Forrest A,Moellering RC Jr,Eliopoulos GM

更新日期：2004-06-01 00:00:00

abstract：:Pulsed-field gel electrophoresis (PFGE) of XbaI-digested chromosomal DNA was performed on 133 strains of Salmonella enterica serovar Typhi obtained from Papua New Guinea, with the objective of assessing the temporal variation of these strains. Fifty-two strains that were isolated in 1992 and 1994 were of one phage typ...

journal_title：Journal of clinical microbiology

authors： Thong KL,Goh YL,Yasin RM,Lau MG,Passey M,Winston G,Yoannes M,Pang T,Reeder JC

更新日期：2002-11-01 00:00:00

abstract：:A female patient suffered a typical attack of typhoid fever due to a lactose-negative, fully susceptible Salmonella typhi strain. During convalescence she became febrile, and a lactose-fermenting S. typhi strain resistant to ampicillin, chloramphenicol, tetracycline, and trimethoprim was isolated from blood culture. T...

journal_title：Journal of clinical microbiology

authors： Cohen SL,Wylie BA,Sooka A,Koornhof HJ

更新日期：1987-08-01 00:00:00

abstract：:Spiroplasma species are organisms that normally colonize plants and insects. We describe the first case of human systemic infection caused by Spiroplasma bacteria in a patient with hypogammaglobulinemia undergoing treatment with biological disease-modifying antirheumatic agents. Spiroplasma turonicum was identified th...

journal_title：Journal of clinical microbiology

authors： Aquilino A,Masiá M,López P,Galiana AJ,Tovar J,Andrés M,Gutiérrez F

更新日期：2015-02-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay for the detection and quantitation of human immunoglobulin M (IgM), IgG, and IgA antibodies against Yersinia enterocolitica is described. Formalinized or heat-treated bacteria were adsorbed onto specially designed microcuvettes, and antibodies were allowed to attach to the antigen-...

journal_title：Journal of clinical microbiology

authors： Granfors K

更新日期：1979-03-01 00:00:00

abstract：:Hybridomas secreting specific monoclonal antibodies (MAb) to all members of the genus Leptospira (clone LF9) and those that are specific only to the pathogenic species (clones LD5 and LE1) were produced. MAb LF9, which was immunoglobulin G1 (IgG1), reacted to a 38-kDa component of the sodium dodecyl sulfate-polyacryla...

journal_title：Journal of clinical microbiology

authors： Saengjaruk P,Chaicumpa W,Watt G,Bunyaraksyotin G,Wuthiekanun V,Tapchaisri P,Sittinont C,Panaphut T,Tomanakan K,Sakolvaree Y,Chongsa-Nguan M,Mahakunkijcharoen Y,Kalambaheti T,Naigowit P,Wambangco MA,Kurazono H,Hayashi H

更新日期：2002-02-01 00:00:00

abstract：:Respiratory diphtheria, characterized by a firmly adherent pseudomembrane, is caused by toxin-producing strains of Corynebacterium diphtheriae, with similar illness produced occasionally by toxigenic Corynebacterium ulcerans or, rarely, Corynebacterium pseudotuberculosis While diphtheria laboratory confirmation requir...

journal_title：Journal of clinical microbiology

authors： Williams MM,Waller JL,Aneke JS,Weigand MR,Diaz MH,Bowden KE,Simon AK,Peng Y,Xiaoli L,Cassiday PK,Winchell J,Tondella ML

更新日期：2020-09-22 00:00:00

abstract：:The coagulation of Limulus amebocyte lysate (LAL) can be activated through two pathways, one initiated by endotoxin and the other by beta-glucans. The two pathways join at the step of activation of the proclotting enzyme. We report here that the endotoxin-activated pathway can be differentially inhibited by two method...

journal_title：Journal of clinical microbiology

authors： Zhang GH,Baek L,Buchardt O,Koch C

更新日期：1994-06-01 00:00:00

abstract：:Because of its low yield in unselected specimens, stool culture is often cost ineffective. We tested 55 fecal samples from Fairfax Hospital (46 patients with diarrhea and 9 from controls without diarrhea) for lactoferrin by latex agglutination (LFLA) with the Leukotest (Techlab, Blacksburg, Va.) as a marker for inflam...

journal_title：Journal of clinical microbiology

authors： Choi SW,Park CH,Silva TM,Zaenker EI,Guerrant RL

更新日期：1996-04-01 00:00:00

abstract：:Enteroviral meningoencephalitis was diagnosed in a patient with an immunodeficiency syndrome acquired after treatment with rituximab for a relapsed primary B-cell lymphoma. A second meningoencephalitic episode was diagnosed 6 months later and was successfully treated with a combination of immunoglobulins and pleconari...

journal_title：Journal of clinical microbiology

authors： Archimbaud C,Bailly JL,Chambon M,Tournilhac O,Travade P,Peigue-Lafeuille H

更新日期：2003-10-01 00:00:00

abstract：:Entamoeba histolytica is an intestinal parasite causing significant morbidity and mortality worldwide. More tools are needed to understand the epidemiology and molecular pathogenesis of amebiasis. A cDNA library was constructed by using poly(A)+ RNA isolated from an axenic strain of E. histolytica, HM1:IMSS, which exp...

journal_title：Journal of clinical microbiology

authors： Burch DJ,Li E,Reed S,Jackson TF,Stanley SL Jr

更新日期：1991-04-01 00:00:00

abstract：:Misidentification of coagulase-negative staphylococci (CoNS) may delay appropriate treatment. We investigated 20 clinical isolates identified as Kocuria spp. by the Vitek 2 system. All were identified as CoNS by 16S rRNA gene sequencing (18 Staphylococcus epidermidis, 1 Staphylococcus haemolyticus). Four Kocuria isola...

journal_title：Journal of clinical microbiology

authors： Ben-Ami R,Navon-Venezia S,Schwartz D,Schlezinger Y,Mekuzas Y,Carmeli Y

更新日期：2005-03-01 00:00:00

abstract：:The potential clinical value of colony counts determined by the lysis-centrifugation blood culture method was studied by reviewing the records of eight patients with persistent septicemia in whom colony counts were available on at least 3 days. Colony counts of the five patients who survived decreased steadily as the ...

journal_title：Journal of clinical microbiology

authors： Whimbey E,Wong B,Kiehn TE,Armstrong D

更新日期：1984-06-01 00:00:00

abstract：:A transrectal prostate biopsy is the most common procedure used to establish the diagnosis of prostate cancer. Prior to biopsy, patients are commonly given ciprofloxacin for prophylaxis. However, a complication of the procedure is infection with ciprofloxacin-resistant organisms, in particular resistant Escherichia co...

journal_title：Journal of clinical microbiology

authors： Liss MA,Nakamura KK,Peterson EM

更新日期：2013-01-01 00:00:00

abstract：:Clostridium difficile is the bacterium most commonly surmised to cause antimicrobial- and hospital-associated diarrhea in developed countries worldwide, and such infections are thought to be increasing in frequency and severity. A laboratory-based study was carried out to characterize C. difficile strains isolated fro...

journal_title：Journal of clinical microbiology

authors： Martin H,Willey B,Low DE,Staempfli HR,McGeer A,Boerlin P,Mulvey M,Weese JS

更新日期：2008-09-01 00:00:00

abstract：:A TaqMan real-time PCR assay, the COBAS TaqMan human immunodeficiency virus (HIV) (HPS-CTMHIV) PCR assay, recently developed for the quantification of HIV type 1 RNA in plasma, was evaluated in comparison with the licensed COBAS AMPLICOR HIV-1 MONITOR (CAHIM) assay. In this study, we have analyzed the tests' sensitivi...

journal_title：Journal of clinical microbiology

authors： Perrin L,Pawlotsky JM,Bouvier-Alias M,Sarrazin C,Zeuzem S,Colucci G

更新日期：2006-12-01 00:00:00

abstract：:In a multicenter study, 113 blood samples from 19 organ transplant patients were analyzed for cytomegalovirus by the pp65 antigenemia assay and a quantitative DNA hybridization assay. Overall, there was 84% agreement among the results obtained by the two tests. Fifteen of 16 episodes of active infection were detected ...

journal_title：Journal of clinical microbiology

authors： Bossart W,Bienz K,Wunderli W

更新日期：1997-12-01 00:00:00

abstract：:We describe a multiplex allele-specific (MAS)-PCR assay to detect simultaneously mutations in the first and third bases of the embB gene codon 306ATG. These mutations are known to confer ethambutol (EMB) resistance in the majority of clinical Mycobacterium tuberculosis isolates worldwide. The mutated bases are reveale...

journal_title：Journal of clinical microbiology

authors： Mokrousov I,Narvskaya O,Limeschenko E,Otten T,Vyshnevskiy B

更新日期：2002-05-01 00:00:00

abstract：:Disk diffusion tests, inoculated directly from positive blood cultures, were evaluated for accuracy of reading zone diameters after 4- and 6-h and overnight incubation. In comparisons with results from standard disk diffusion tests, the 4-h results were in agreement for 83% of tests with gram-positive organisms and 64...

journal_title：Journal of clinical microbiology

authors： Coyle MB,McGonagle LA,Plorde JJ,Clausen CR,Schoenknecht FD

更新日期：1984-09-01 00:00:00

abstract：:Acinetobacter spp. are important nosocomial pathogens reported with increasing frequency in outbreaks of cross-infection during the past 2 decades. The majority of such outbreaks are caused by Acinetobacter baumannii. To investigate whether desiccation tolerance may be involved in the ability of certain strains of A. ...

journal_title：Journal of clinical microbiology

authors： Jawad A,Seifert H,Snelling AM,Heritage J,Hawkey PM

更新日期：1998-07-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay was developed to study the subclass distribution of immunoglobulin G (IgG) specific to the core glycolipid (CGL) of the Re mutant of Salmonella minnesota R595 in serum samples from individuals with an IgG response toward these antigens. In a group of healthy blood donors, we detect...

journal_title：Journal of clinical microbiology

authors： Nys M,Joassin L,Somzee A,Demonty J

更新日期：1988-05-01 00:00:00

abstract：:The staphylococcal methicillin resistance determinant, mecA, resides on a mobile genetic element, staphylococcus chromosomal cassette mec (SCCmec). The distribution of SCCmec in nature is limited to relatively few clonal complexes of related methicillin-resistant Staphylococcus aureus (MRSA). We have previously report...

journal_title：Journal of clinical microbiology

authors： Katayama Y,Robinson DA,Enright MC,Chambers HF

更新日期：2005-05-01 00:00:00

abstract：:The replication of human coronavirus strain 229E was observed by using indirect immunofluorescence in infected monolayers of MRC continuous cells. By 8 h after infection, bright cytoplasmic fluorescence was detected in cells infected with human coronavirus 229E. Discrete foci of infection were observed from 8 to 16 h ...

journal_title：Journal of clinical microbiology

authors： Macnaughton MR,Thomas BJ,Davies HA,Patterson S

更新日期：1980-09-01 00:00:00

abstract：:Clinical isolates of group B streptococci from body fluids and mucosal surfaces were examined for production of a trypsin-resistant antigen known as R protein. R protein was extracted with 1% trypsin from cells grown in a semidefined medium. The extracts were tested by immunodiffusion in agarose with a panel of antise...

journal_title：Journal of clinical microbiology

authors： Flores AE,Ferrieri P

更新日期：1989-05-01 00:00:00

abstract：:Human bocavirus 1 (HBoV1) is associated with respiratory infections worldwide, mainly in children. Similar to other parvoviruses, it is believed that HBoV1 can persist for long periods of time in humans, probably through maintaining concatemers of the virus single-stranded DNA genome in the nuclei of infected cells. R...

journal_title：Journal of clinical microbiology

authors： Proenca-Modena JL,Paula FE,Buzatto GP,Carenzi LR,Saturno TH,Prates MC,Silva ML,Delcaro LS,Valera FC,Tamashiro E,Anselmo-Lima WT,Arruda E

更新日期：2014-08-01 00:00:00

abstract：:We evaluated a newly commercial enzyme immunoassay (EIA) (Biotest Legionella Urin Antigen EIA; Biotest AG, Dreieich, Germany) for detection of antigens of all Legionella pneumophila serogroups with a relatively wide spectrum of cross-reactivity as well as antigens of other Legionella spp. by comparing its sensitivity ...

journal_title：Journal of clinical microbiology

authors： Domínguez JA,Galí N,Pedroso P,Fargas A,Padilla E,Manterola JM,Matas L

更新日期：1998-09-01 00:00:00

abstract：:We report the evaluation of recombinant severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) nucleocapsid protein enzyme-linked immunosorbent assay (ELISA)-based antibody tests for serodiagnosis of SARS-CoV pneumonia and compare the sensitivities and specificities of this ELISA for detection of immunoglobul...

journal_title：Journal of clinical microbiology

authors： Woo PC,Lau SK,Wong BH,Tsoi HW,Fung AM,Chan KH,Tam VK,Peiris JS,Yuen KY

更新日期：2004-05-01 00:00:00

abstract：:Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human corona...

journal_title：Journal of clinical microbiology

authors： Chan JF,Choi GK,Tsang AK,Tee KM,Lam HY,Yip CC,To KK,Cheng VC,Yeung ML,Lau SK,Woo PC,Chan KH,Tang BS,Yuen KY

更新日期：2015-08-01 00:00:00

abstract：:Fluorophore-assisted carbohydrate electrophoresis (FACE) is a straightforward, sensitive method for determining the presence and relative abundance of individual oligomannosyl residues in Candida mannoprotein, the major antigenic determinant located on the outer surface of the yeast cell wall. The single terminal alde...

journal_title：Journal of clinical microbiology

authors： Goins TL,Cutler JE

更新日期：2000-08-01 00:00:00