Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnosis of SARS coronavirus pneumonia.

Abstract:

:We report the evaluation of recombinant severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) nucleocapsid protein enzyme-linked immunosorbent assay (ELISA)-based antibody tests for serodiagnosis of SARS-CoV pneumonia and compare the sensitivities and specificities of this ELISA for detection of immunoglobulin G (IgG), IgM, IgA, and their combinations with serum samples from 149 healthy blood donors who donated blood 3 years ago as controls and 106 SARS-CoV pneumonia patients in Hong Kong. The specificities of the ELISA for IgG, IgM, and IgA detection were 95.3, 96.6, and 96.6%, respectively, with corresponding sensitivities of 94.3, 59.4, and 60.4%, respectively. The present ELISA appears to be a sensitive test for serodiagnosis of SARS-CoV pneumonia, is much more economical and less labor-intensive than the indirect immunofluorescence assay, and does not require cultivation of SARS-CoV.

journal_name

J Clin Microbiol

authors

Woo PC,Lau SK,Wong BH,Tsoi HW,Fung AM,Chan KH,Tam VK,Peiris JS,Yuen KY

doi

10.1128/jcm.42.5.2306-2309.2004

subject

Has Abstract

pub_date

2004-05-01 00:00:00

pages

2306-9

issue

5

eissn

0095-1137

issn

1098-660X

journal_volume

42

pub_type

杂志文章
  • Carriage frequency, intensity of carriage, and strains of oral yeast species vary in the progression to oral candidiasis in human immunodeficiency virus-positive individuals.

    abstract::Candida samples were taken over a period of 2 years from 54 human immunodeficiency virus (HIV)-positive asymptomatic subjects to evaluate changes in yeast carriage, intensity of carriage, and genotype over time. Overall, we found that HIV-positive patients with CD4(+)-cell counts of between 200 and 400/microl had sign...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.40.2.341-350.2002

    authors: Vargas KG,Joly S

    更新日期:2002-02-01 00:00:00

  • Detection and quantification of latently infected B lymphocytes in Epstein-Barr virus-seropositive, healthy individuals by polymerase chain reaction.

    abstract::We designed a highly sensitive and specific polymerase chain reaction assay for the detection of Epstein-Barr virus (EBV)-related sequences in B-cell DNA of EBV-seropositive healthy individuals. By using this assay, we were able to amplify at least 10 copies of a plasmid containing the BamHI-W region, which is repeate...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.11.2826-2829.1992

    authors: Wagner HJ,Bein G,Bitsch A,Kirchner H

    更新日期:1992-11-01 00:00:00

  • Identification of spirochetes associated with contagious ovine digital dermatitis.

    abstract::Spirochetes of the genus Treponema were cultured from 7 of 10 cases of digital dermatitis in sheep. Two cultures comprised Treponema phagedenis-like and Treponema medium/Treponema vincentii-like spirochetes, respectively, while the remaining cultures comprised mixed populations of Treponema medium/Treponema vincentii-...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01934-08

    authors: Sayers G,Marques PX,Evans NJ,O'Grady L,Doherty ML,Carter SD,Nally JE

    更新日期:2009-04-01 00:00:00

  • Multicenter evaluation of the Quidel Lyra Direct C. difficile nucleic acid amplification assay.

    abstract::Clostridium difficile is a Gram-positive bacterium commonly found in health care and long-term-care facilities and is the most common cause of antibiotic-associated diarrhea. Rapid detection of this bacterium can assist physicians in implementing contact precautions and appropriate antibiotic therapy in a timely manne...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究

    doi:10.1128/JCM.03089-13

    authors: Beck ET,Buchan BW,Riebe KM,Alkins BR,Pancholi P,Granato PA,Ledeboer NA

    更新日期:2014-06-01 00:00:00

  • Dot blot assay for the serotyping of pneumococci.

    abstract::To simplify the serotyping of Streptococcus pneumoniae, a dot bot assay has been developed and compared with the standard quellung reaction in 1,082 isolates. The technique has been demonstrated to be sensitive, specific, easy to perform, and inexpensive. The dot blot assay could be useful when large numbers of pneumo...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.35.3.764-766.1997

    authors: Fenoll A,Jado I,Vicioso D,Casal J

    更新日期:1997-03-01 00:00:00

  • Catheter-related sepsis due to Rhodotorula glutinis.

    abstract::We describe a central venous catheter-related (Port-A-Cath; Smiths Industries Medical Systems [SIMS] Deltec, Inc., St. Paul, Minn.) infection caused by Rhodotorula glutinis in a 51-year-old man with nasopharyngeal carcinoma. He was treated with fluconazole for 8 weeks and had the catheter removed. Two isolates of R. g...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.2.857-859.2003

    authors: Hsueh PR,Teng LJ,Ho SW,Luh KT

    更新日期:2003-02-01 00:00:00

  • Comparison of self-collected and physician-collected vaginal swabs for microbiome analysis.

    abstract::To our knowledge, no data are available on whether the microbial species composition and abundance sampled with self-collected vaginal swabs are comparable to those of swabs collected by clinicians. Twenty healthy women were recruited to the study during a routine gynecological visit. Eligible women were between 18 an...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01710-09

    authors: Forney LJ,Gajer P,Williams CJ,Schneider GM,Koenig SS,McCulle SL,Karlebach S,Brotman RM,Davis CC,Ault K,Ravel J

    更新日期:2010-05-01 00:00:00

  • Novel method for genotyping hepatitis B virus on the basis of TaqMan real-time PCR.

    abstract::Chronic infection with hepatitis B virus (HBV) is an important cause of cirrhosis and cancer of the liver. HBV is currently classified into eight genotypes, A to H. Accumulated evidence shows that the genotype influences both the clinical course of infection and the response to treatment. We describe a new method for ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01442-09

    authors: Malmström S,Berglin-Enquist I,Lindh M

    更新日期:2010-04-01 00:00:00

  • A space-saving media table.

    abstract::A space-saving media table designed to facilitate pouring large quantities of plated media in a small area is described. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: McGonagle LA,Schmidt G,Eng S,Wirsing K

    更新日期:1975-07-01 00:00:00

  • Sensitivity and specificity of viral immunoglobulin M determination by indirect enzyme-linked immunosorbent assay.

    abstract::Four sources of error associated with virus-specific immunoglobulin M (IgM) determination by indirect enzyme-linked immunosorbent assay were recognized and analyzed. First, competitive inhibition due to specific IgG was demonstrated by experiments involving addition and subtraction of rubella-specific IgG. Second, the...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.26.2.328-332.1988

    authors: Champsaur H,Fattal-German M,Arranhado R

    更新日期:1988-02-01 00:00:00

  • Genotype-specific clearance of genital human papillomavirus (HPV) infections among mothers in the Finnish family HPV study.

    abstract::The majority of cervical human papillomavirus (HPV) infections in young women are transient, but whether the clearance differs among different HPV genotypes and the different factors predicting genotype-specific clearance are partly unknown. In the Finnish Family HPV Study, 131 of 252 women (mean age, 25.5 years) clea...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00783-10

    authors: Louvanto K,Syrjänen KJ,Rintala MA,Grénman SE,Syrjänen SM

    更新日期:2010-08-01 00:00:00

  • Clinical use of 16S rRNA gene sequencing to identify Mycoplasma felis and M. gateae associated with feline ulcerative keratitis.

    abstract::Routine bacterial cultures of corneal scrapings from seven cats with either ulcerative feline keratitis, keratomalacia, or both yielded colonies which were identified by 16S rRNA gene sequencing as Mycoplasma felis (six cases) and Mycoplasma gateae (one case). Identification of the pathogens allowed the use of less em...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.7.3431-3434.2005

    authors: Gray LD,Ketring KL,Tang YW

    更新日期:2005-07-01 00:00:00

  • Spread of a Salmonella typhimurium clone resistant to expanded-spectrum cephalosporins in three European countries.

    abstract::Twelve Salmonella typhimurium strains resistant to broad-spectrum cephalosporins were isolated from cases of gastroenteritis during 1996 to 1998 in Russia, Hungary, and Greece. Resistance was due to the production of CTX-M-type extended-spectrum beta-lactamases encoded by similar 12-kb plasmids. By pulsed-field gel el...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.11.3774-3777.1999

    authors: Tassios PT,Gazouli M,Tzelepi E,Milch H,Kozlova N,Sidorenko S,Legakis NJ,Tzouvelekis LS

    更新日期:1999-11-01 00:00:00

  • The recombinant 120-kilodalton protein of Ehrlichia chaffeensis, a potential diagnostic tool.

    abstract::DNA encoding two repeat units of 120-kDa protein of Ehrlichia chaffeensis was cloned into the expression vector pGEX and expressed in Escherichia coli. The sensitivity and specificity of a dot blot assay for detection of human antibodies with the recombinant protein were 86 and 100%, respectively, compared with an ind...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.34.11.2853-2855.1996

    authors: Yu XJ,Crocquet-Valdes P,Cullman LC,Walker DH

    更新日期:1996-11-01 00:00:00

  • Direct fluorescent-antibody confirmation of chlamydial antigen below the detection threshold of the chlamydiazyme enzyme-linked immunosorbent assay.

    abstract::Of 4,000 endocervical specimens tested with the Chlamydiazyme enzyme-linked immunosorbent assay (Abbott Laboratories), 233 (5.8%) gave positive results (A492 above the cutoff), which were confirmed with a blocking reagent (Abbott). An additional 34 specimens (14.6%) with chlamydial antigen were detected and confirmed ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.31.6.1646-1647.1993

    authors: Kellogg JA,Seiple JW,Stroll ES

    更新日期:1993-06-01 00:00:00

  • Role of a cefoxitin-inducible beta-lactamase in a case of breakthrough bacteremia.

    abstract::Development of resistance during therapy with cefamandole contributes to treatment failure. A simple cefoxitin disk test was recently described which detects a cefamandole-active inducible beta-lactamase not otherwise detectable with cefamandole as the inducer. A case of breakthrough Enterobacter bacteremia due to se...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.12.4.517-520.1980

    authors: Beckwith DG,Jahre JA

    更新日期:1980-10-01 00:00:00

  • Molecular subtyping and characterization of bovine and human Streptococcus agalactiae isolates.

    abstract::Streptococcus agalactiae causes severe invasive disease in humans and mastitis in cattle. Temporally matched bovine milk isolates and clinical human invasive isolates (52 each) collected in New York State over 18 months were characterized by molecular subtyping and phenotypic methods to probe the interspecies transmis...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.3.1177-1186.2005

    authors: Sukhnanand S,Dogan B,Ayodele MO,Zadoks RN,Craver MP,Dumas NB,Schukken YH,Boor KJ,Wiedmann M

    更新日期:2005-03-01 00:00:00

  • Detection of pathogenic Yersinia enterocolitica by using congo red-magnesium oxalate agar medium.

    abstract::A Congo red-magnesium oxalate agar medium was developed to detect expression of virulence-associated calcium dependency and Congo red absorption in Yersinia enterocolitica. Of the 157 pathogenic serotypes tested, 119 (75.8%) were positive; 98% of nonpathogenic serotypes and strains of three other Yersinia species were...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.27.1.213-214.1989

    authors: Riley G,Toma S

    更新日期:1989-01-01 00:00:00

  • Detection of immunoglobulin G to Pasteurella haemolytica capsular polysaccharide by enzyme-linked immunosorbent assay.

    abstract::An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of immunoglobulin G (IgG) to the capsular polysaccharide (CP) of Pasteurella haemolytica serotype 1. Purified CP was first covalently coupled to poly-L-lysine and then optimally adsorbed at a concentration of 5 micrograms/ml to microtiter pla...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.25.2.242-247.1987

    authors: Townsend EL,Maheswaran SK,Leininger JR,Ames TR

    更新日期:1987-02-01 00:00:00

  • Multilocus variable-number tandem-repeat polymorphism among Brazilian Enterococcus faecalis strains.

    abstract::Multilocus variable-number tandem-repeat analysis (MLVA) for seven genomic loci was developed for Enterococcus faecalis. MLVA and pulsed-field gel electrophoresis (PFGE) resulted in 37 and 31 genotypes among 83 strains, respectively. Both typing schemes were highly concordant (90.4%). MLVA is an excellent alternative ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.42.10.4879-4881.2004

    authors: Titze-de-Almeida R,Willems RJ,Top J,Rodrigues IP,Ferreira RF 2nd,Boelens H,Brandileone MC,Zanella RC,Felipe MS,van Belkum A

    更新日期:2004-10-01 00:00:00

  • Evaluation of pooling strategies for acute HIV-1 infection screening using nucleic acid amplification testing.

    abstract::A 128-member specimen pooling scheme for acute HIV infection (AHI) detection was evaluated using 21 AHI specimens (range, 1,520 to 500,000 copies/ml) previously identified by RNA testing of 16-member plasma pools. HIV-1 RNA was detectable in 128-member pools for all 21 specimens; however, one pool created from a speci...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00650-11

    authors: Sullivan TJ,Patel P,Hutchinson A,Ethridge SF,Parker MM

    更新日期:2011-10-01 00:00:00

  • Sequence variation in the small-subunit rRNA gene of Plasmodium malariae and prevalence of isolates with the variant sequence in Sichuan, China.

    abstract::By two PCR-based diagnostic methods, Plasmodium malariae infections have been rediscovered at two foci in the Sichuan province of China, a region where no cases of P. malariae have been officially reported for the last 2 decades. In addition, a variant form of P. malariae which has a deletion of 19 bp and seven substi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.36.11.3378-3381.1998

    authors: Liu Q,Zhu S,Mizuno S,Kimura M,Liu P,Isomura S,Wang X,Kawamoto F

    更新日期:1998-11-01 00:00:00

  • Clinical evaluation of the BDProbeTec ET system for rapid detection of Mycobacterium tuberculosis.

    abstract::The performance of the BDProbeTec ET system (BD Biosciences, Sparks, Md.) for direct detection of Mycobacterium tuberculosis complex (MTBC) in respiratory specimens was evaluated by comparing results to those of conventional mycobacterial culture performed with the BACTEC 460 TB system and Middlebrook 7H11 biplates. P...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.38.2.863-865.2000

    authors: Bergmann JS,Keating WE,Woods GL

    更新日期:2000-02-01 00:00:00

  • Immunoglobulin M capture immunoassay in investigation of coxsackievirus B5 and B6 outbreaks in South Australia.

    abstract::An immunoglobulin M (IgM) capture enzyme immunoassay was used to detect major overlapping outbreaks of disease in South Australia caused by coxsackieviruses B5 (CBV-5) and B6 (CBV-6). CBV-5-specific IgM was detected in patients presenting in spring 1992 with acute febrile illnesses, rash, severe acute respiratory dise...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.6.1628-1631.1995

    authors: Goldwater PN

    更新日期:1995-06-01 00:00:00

  • Quantitative detection of Neospora caninum in bovine aborted fetuses and experimentally infected mice by real-time PCR.

    abstract::We report the development of a real-time PCR assay for the quantitative detection of Neospora caninum in infected host tissues. The assay uses the double-stranded DNA-binding dye SYBR Green I to continuously monitor product formation. Oligonucleotide primers were designed to amplify a 76-bp DNA fragment corresponding ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.40.4.1194-1198.2002

    authors: Collantes-Fernández E,Zaballos A,Alvarez-García G,Ortega-Mora LM

    更新日期:2002-04-01 00:00:00

  • Evaluation of BacT/ALERT system for detection of Mycoplasma hominis in simulated blood cultures.

    abstract::We used simulated blood cultures inoculated with clinical isolates of Mycoplasma hominis to determine whether liquid media of the BacT/ALERT (Organon Teknika, Durham, N.C.) will support growth of this fastidious organism and whether its presence can generate a positive signal with the instrument. Viability of clinical...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.39.12.4328-4331.2001

    authors: Waites KB,Canupp KC

    更新日期:2001-12-01 00:00:00

  • Diagnosis of human metapneumovirus infection in immunosuppressed lung transplant recipients and children evaluated for pertussis.

    abstract::Human metapneumovirus (hMPV) is a recently discovered paramyxovirus that is known to cause respiratory tract infections in children and immunocompromised individuals. Given the difficulties of identifying hMPV by conventional culture, molecular techniques could improve the detection of this virus in clinical specimens...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01621-06

    authors: Dare R,Sanghavi S,Bullotta A,Keightley MC,George KS,Wadowsky RM,Paterson DL,McCurry KR,Reinhart TA,Husain S,Rinaldo CR

    更新日期:2007-02-01 00:00:00

  • Clinical significance of mites in urine.

    abstract::We report a case where a mite egg found in urine caused diagnostic confusion. The possibility of gut or bladder mite infection should be entertained only after repeated identification of mites in urine or stool samples from a symptomatic patient with no other cause for the symptoms and where the possibilities of conta...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.12.6200-6201.2005

    authors: Dini LA,Frean JA

    更新日期:2005-12-01 00:00:00

  • Rapid, simple method of preparing rotaviral double-stranded ribonucleic acid for analysis by polyacrylamide gel electrophoresis.

    abstract::A procedure for extracting rotaviral double-stranded ribonucleic acid (RNA) directly from fecal and intestinal specimens collected from calves and pigs is described. This procedure provides a rapid, simple, reproducible method of obtaining rotaviral double-stranded RNA preparations suitable for electrophoretic analysi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.14.3.273-280.1981

    authors: Theil KW,McCloskey CM,Saif LJ,Redman DR,Bohl EH,Hancock DD,Kohler EM,Moorhead PD

    更新日期:1981-09-01 00:00:00

  • Mixed bacterial meningitis due to Streptococcus pneumoniae and Neisseria meningitidis in an 18-month-old child.

    abstract::We report an unusual case of culture-proven pneumococcal and meningococcal mixed meningitis in an 18-month-old girl. The patient responded well to antimicrobial therapy and recovered completely without sequelae. No underlying condition could be demonstrated except a rhinitis of unknown etiology 2 days before the onset...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,评审

    doi:10.1128/JCM.43.3.1477-1479.2005

    authors: Marchandin H,Ventura V,Alonso JM,Van de Perre P

    更新日期:2005-03-01 00:00:00