Comparison of broth enhancement to direct plating for screening of rectal cultures for ciprofloxacin-resistant Escherichia coli.

abstract：:Several methods have been previously suggested for the presumptive identification of Gardnerella vaginalis in clinical laboratories, but none is entirely satisfactory. We previously found that sodium polyanetholesulfonate (SPS) inhibits G. vaginalis in blood culture media. We compared susceptibility to an SPS-containi...

journal_title：Journal of clinical microbiology

authors： Reimer LG,Reller LB

更新日期：1985-02-01 00:00:00

abstract：:This study compared the abilities of API 20E and BBL Crystal E/NF identification systems to correctly identify human and animal source gram-negative bacilli of known identifications, as provided by the American Type Culture Collection, Rockville, Md., and the Research Diagnostic and Investigative Laboratory, Columbia,...

journal_title：Journal of clinical microbiology

authors： Peele D,Bradfield J,Pryor W,Vore S

更新日期：1997-01-01 00:00:00

abstract：:We describe the serum immunoglobulin A (IgA) antibody response to Norwalk virus infection in human volunteers and compare it with previously described IgM and total antibody responses. Whereas specific IgA and IgM peak within 2 weeks after onset of symptoms, titers of total blocking antibody continue to rise, implying...

journal_title：Journal of clinical microbiology

authors： Erdman DD,Gary GW,Anderson LJ

更新日期：1989-06-01 00:00:00

abstract：:The performance of a gp41 assay, created using reagents designed for use with the OpenGene DNA Sequencing System, was evaluated using a panel of plasma samples obtained from enfuvirtide-naive and -experienced human immunodeficiency virus type 1-infected subjects. Resulting sequence data were highly accurate compared t...

journal_title：Journal of clinical microbiology

authors： Marcial M,Lu J,Deeks SG,Ziermann R,Kuritzkes DR

更新日期：2006-09-01 00:00:00

abstract：:A total of 50 strains of Staphylococcus aureus, including 41 methicillin-resistant S. aureus (MRSA) strains, were characterized by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins. The protein patterns contained 40-50 discrete bands and were highly reproducible. Partial ...

journal_title：Journal of clinical microbiology

authors： Costas M,Cookson BD,Talsania HG,Owen RJ

更新日期：1989-11-01 00:00:00

abstract：:Phenolphthalein diphosphate was incorporated into a primary blood agar medium for use in performing quantitative urine cultures. Phosphatase-negative staphylococci, such as Staphylococcus saprophyticus, were differentiated from phosphatase-positive species, such as Staphylococcus epidermidis, by spot testing colonies ...

journal_title：Journal of clinical microbiology

authors： Pickett DA,Welch DF

更新日期：1985-03-01 00:00:00

abstract：:A rapid screening test has been developed to detect heat-labile enterotoxin produced by individual colonies of Escherichia coli or Vibrio cholerae growing on media solidified with agar. The applicability of this method for isolating tox mutants of E. coli and V. cholerae has been demonstrated. ...

journal_title：Journal of clinical microbiology

authors： Bramucci MG,Holmes RK

更新日期：1978-08-01 00:00:00

abstract：:A 55-year-old man with severe peripheral vascular disease developed nosocomial septicemia which was caused by the gram-negative bacterium CDC group IV c-2, presumably from a plantar abscess on the left foot. Recovery followed amputation of the infected extremity and antibiotic therapy. This is the first reported case ...

journal_title：Journal of clinical microbiology

authors： Crowe HM,Brecher SM

更新日期：1987-11-01 00:00:00

abstract：:A group of 76 consecutive human immunodeficiency virus (HIV)-positive patients with fever of unknown origin (n = 52) or fever associated with pulmonary diseases was evaluated in order to assess the usefulness of PCR with peripheral blood in the diagnosis and follow-up of visceral leishmaniasis. We identified 10 cases ...

journal_title：Journal of clinical microbiology

authors： Pizzuto M,Piazza M,Senese D,Scalamogna C,Calattini S,Corsico L,Persico T,Adriani B,Magni C,Guaraldi G,Gaiera G,Ludovisi A,Gramiccia M,Galli M,Moroni M,Corbellino M,Antinori S

更新日期：2001-01-01 00:00:00

abstract：:We report herein a case of Bartholin's gland abscess caused by Brucella melitensis. Clinical microbiology laboratory workers in areas where this disease is endemic should be familiar with the bacteriological features of this organism and consider the possibility of a brucellar etiology in a broad range of clinical set...

journal_title：Journal of clinical microbiology

authors： Peled N,David Y,Yagupsky P

更新日期：2004-02-01 00:00:00

abstract：:Twelve bacterial species including Streptococcus uberis, S. parauberis, S. agalactiae, S. dysgalactiae, S. bovis, S. mitis, S. salivarius, S. saccharolyticus, Enterococcus faecium, E. faecalis, E. avium, and Aerococcus viridans were examined for their 16S ribosomal DNA fingerprint patterns. Oligonucleotide primers com...

journal_title：Journal of clinical microbiology

authors： Jayarao BM,Doré JJ Jr,Oliver SP

更新日期：1992-09-01 00:00:00

abstract：:Accurate identification of mycobacterial species and subspecies is essential to evaluate their significance and to perform epidemiological studies. The subspecies of Mycobacterium avium have different attributes but coincide in their zoonotic potential. Our knowledge about M. avium subsp. silvaticum is limited, since ...

journal_title：Journal of clinical microbiology

authors： Rónai Z,Csivincsik Á,Dán Á

更新日期：2015-05-01 00:00:00

abstract：:A differential staining method employing acridine orange-stained rickettsiae and safranin-stained standardized suspensions of Shigella dysenteriae is described for enumerating rickettsiae harvested during various stages of the growth cycle. ...

journal_title：Journal of clinical microbiology

authors： Silverman DJ,Fiset P,Wisseman CL Jr

更新日期：1979-03-01 00:00:00

abstract：:A novel PCR detection assay that amplifies the Helicobacter pylori-specific vacuolating cytotoxin gene (vacA) and thus enables rapid diagnosis of infection is described. Additionally, a real-time probe hybridization melting point analysis assay to detect all three mutations in the 23S rRNA gene associated with clarith...

journal_title：Journal of clinical microbiology

authors： Chisholm SA,Owen RJ,Teare EL,Saverymuttu S

更新日期：2001-04-01 00:00:00

abstract：:Herpes simplex virus (HSV) infections are very common in the general population and among immunocompromised patients. Acyclovir (ACV) is an effective treatment which is widely used. We deemed it essential to conduct a wide and coordinated survey of the emergence of ACV-resistant HSV strains. We have formed a network o...

journal_title：Journal of clinical microbiology

authors： Danve-Szatanek C,Aymard M,Thouvenot D,Morfin F,Agius G,Bertin I,Billaudel S,Chanzy B,Coste-Burel M,Finkielsztejn L,Fleury H,Hadou T,Henquell C,Lafeuille H,Lafon ME,Le Faou A,Legrand MC,Maille L,Mengelle C,Morand P,

更新日期：2004-01-01 00:00:00

abstract：:The Alere i Influenza A&B assay is a newly developed rapid molecular assay which has the potential to generate results within 15 min from sample collection. In this study, we evaluated the Alere i Influenza A&B assay by using salvaged frozen respiratory specimens that were collected in viral transport medium from chil...

journal_title：Journal of clinical microbiology

authors： Bell JJ,Selvarangan R

更新日期：2014-11-01 00:00:00

abstract：:The impact of the viability of Trichomonas vaginalis in urine on wet mount, culture, and PCR methods was assessed. To minimize the chance of false-negative results, urine specimens should be processed within 30 min of specimen collection and maintained at 37 degrees C, since temperature appears to affect the viability...

journal_title：Journal of clinical microbiology

authors： Shafir SC,Sorvillo FJ

更新日期：2006-10-01 00:00:00

abstract：:An automated cultivation system for mycobacteria, the MGIT 960 system (MGIT system), was compared in the clinical routine with two variants of Löwenstein-Jensen (L-J) medium. A total of 152 isolates were recovered from 2,015 specimens: 139 (91%) with the MGIT system and 127 (84%) with L-J media (P = 0.05). These inclu...

journal_title：Journal of clinical microbiology

authors： Katila ML,Katila P,Erkinjuntti-Pekkanen R

更新日期：2000-03-01 00:00:00

abstract：:Three hundred thirty-one yeast and yeast-like isolates were cultivated on eosin methylene blue agar. While the sensitivity rate for Candida kefyr isolates producing a metallic green sheen was 81.8%, the high positive predictive value (100%) for yeast isolates with this phenotype belonging to C. kefyr suggests that the...

journal_title：Journal of clinical microbiology

authors： Munson EL,Troy DR,Weber JK,Messer SA,Pfaller MA

更新日期：2002-11-01 00:00:00

abstract：:Of 45 influenza virus strains (43 type A and 2 type B) detected in conventional tube cell cultures (average time, 4 days), 25 (56%) were detected by immunofluorescence in the shell vial assay 24 h postinoculation. The specific fluorescence produced should allow this procedure to be readily adapted by laboratories with...

journal_title：Journal of clinical microbiology

authors： Espy MJ,Smith TF,Harmon MW,Kendal AP

更新日期：1986-10-01 00:00:00

abstract：:During February and March 1984, 207 fecal samples from infants and children with gastroenteritis were tested for rotavirus with four techniques: two enzyme immunoassays (Rotazyme; Abbott Laboratories, North Chicago, Ill., and Enzygnost-Rotavirus; Calbiochem-Behring, La Jolla, Calif.) and two latex agglutination tests ...

journal_title：Journal of clinical microbiology

authors： Sambourg M,Goudeau A,Courant C,Pinon G,Denis F

更新日期：1985-04-01 00:00:00

abstract：:By two PCR-based diagnostic methods, Plasmodium malariae infections have been rediscovered at two foci in the Sichuan province of China, a region where no cases of P. malariae have been officially reported for the last 2 decades. In addition, a variant form of P. malariae which has a deletion of 19 bp and seven substi...

journal_title：Journal of clinical microbiology

authors： Liu Q,Zhu S,Mizuno S,Kimura M,Liu P,Isomura S,Wang X,Kawamoto F

更新日期：1998-11-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) strains display genetic polymorphisms, and these polymorphisms can be analyzed to study viral transmission and pathogenesis. Recently, short tandem repeat (STR) length polymorphisms have been identified in the HCMV genome. We assessed the utility of STRs in characterizing HCMV strains and ...

journal_title：Journal of clinical microbiology

authors： Walker A,Petheram SJ,Ballard L,Murph JR,Demmler GJ,Bale JF Jr

更新日期：2001-06-01 00:00:00

abstract：:The use of a new commercial separation column on whole test serum improved the method for detecting immunoglobulin M antibody to Epstein-Barr virus. The efficiency of this product in absorbing interfering immunoglobulin G was similar to that of another absorption product, staphylococcal protein A, but it has the advan...

journal_title：Journal of clinical microbiology

authors： Sumaya CV,Ench Y,Carrillo MA,Pope RM

更新日期：1984-08-01 00:00:00

abstract：:The complete nucleotide sequence of one of the immediate-early genes of human herpesvirus 6 variant B was determined and compared with that of variant A reported by Martin et al. (M.D. Martin, J. Nicholas, B. J. Thomson, C. Newman, and R. W. Honess, J. Virol. 65:5381-5390, 1991). While it was reported that two open re...

journal_title：Journal of clinical microbiology

authors： Yamamoto T,Mukai T,Kondo K,Yamanishi K

更新日期：1994-02-01 00:00:00

abstract：:Serum samples collected from 68 patients (age, mean +/- the standard deviation [SD], 56.3 +/- 12.8 years) at admission who were subsequently molecularly diagnosed as having hepatitis E and from 2,781 individuals who were assumed not to have been recently infected with hepatitis E virus (HEV; negative controls; 52.9 +/...

journal_title：Journal of clinical microbiology

authors： Takahashi M,Kusakai S,Mizuo H,Suzuki K,Fujimura K,Masuko K,Sugai Y,Aikawa T,Nishizawa T,Okamoto H

更新日期：2005-01-01 00:00:00

abstract：:The genetic and phylogenetic characteristics of human enterovirus 71 (EV71) and coxsackievirus A16 (CA16) sampled from children with hand, foot, and mouth disease in Shenzhen, People's Republic of China, over a 6-year period (1999 to 2004) were examined with reverse transcription-PCR and DNA sequencing. Out of 147 sto...

journal_title：Journal of clinical microbiology

authors： Li L,He Y,Yang H,Zhu J,Xu X,Dong J,Zhu Y,Jin Q

更新日期：2005-08-01 00:00:00

abstract：:Leifsonia aquatica is an aquatic bacterium that is typically found in environmental water habitats. Infections due to L. aquatica are rare and commonly catheter associated in immunocompromised patients. We report the first case of an acute septicemia caused by L. aquatica in a healthy immunocompetent host after cryope...

journal_title：Journal of clinical microbiology

authors： Han L,Lei JE,Wang X,Guo LT,Kang QY,He L,Xu JR

更新日期：2013-11-01 00:00:00

abstract：:The timely and exact diagnosis of prosthetic joint infection (PJI) is crucial for surgical decision-making. Intraoperatively, delivery of the result within an hour is required. Alpha-defensin lateral immunoassay of joint fluid (JF) is precise for the intraoperative exclusion of PJI; however, for patients with a limite...

journal_title：Journal of clinical microbiology

authors： Fillerova R,Gallo J,Radvansky M,Kraiczova V,Kudelka M,Kriegova E

更新日期：2017-09-01 00:00:00

abstract：:The Clinical and Laboratory Standards Institute (CLSI) has revised several breakpoints since 2010 for bacteria that grow aerobically. In 2019, these revisions include changes to the ciprofloxacin and levofloxacin breakpoints for the Enterobacteriaceae and Pseudomonas aeruginosa, daptomycin breakpoints for Enterococcus...

journal_title：Journal of clinical microbiology

authors： Humphries RM,Abbott AN,Hindler JA

更新日期：2019-05-24 00:00:00