Understanding and Addressing CLSI Breakpoint Revisions: a Primer for Clinical Laboratories.

abstract：:An enzyme-linked immunosorbent assay for the detection and quantitation of human immunoglobulin M (IgM), IgG, and IgA antibodies against Yersinia enterocolitica is described. Formalinized or heat-treated bacteria were adsorbed onto specially designed microcuvettes, and antibodies were allowed to attach to the antigen-...

journal_title：Journal of clinical microbiology

authors： Granfors K

更新日期：1979-03-01 00:00:00

abstract：:Infection of calves with bovine herpesvirus 1 (BHV-1) results in transient immunosuppression that may lead to bacterium-induced pneumonia and, occasionally, death. Although sensory neurons in the trigeminal ganglia (TG) are the primary site of BHV-1 latency, viral genomes are detected in the tonsils of latently infect...

journal_title：Journal of clinical microbiology

authors： Perez S,Inman M,Doster A,Jones C

更新日期：2005-01-01 00:00:00

abstract：:We report an outbreak of Saccharomyces cerevisiae subtype boulardii fungemia among three intensive care unit roommates of patients receiving lyophilized preparations of this fungus. The fungemia was probably due to central venous catheter contamination and resolved after fluconazole treatment. The need for stringent a...

journal_title：Journal of clinical microbiology

authors： Cassone M,Serra P,Mondello F,Girolamo A,Scafetti S,Pistella E,Venditti M

更新日期：2003-11-01 00:00:00

abstract：:Sequence analysis of the internal transcribed spacer (ITS) region was employed as the gold standard method for yeast identification in the China Hospital Invasive Fungal Surveillance Net (CHIF-NET). It has subsequently been found that matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-...

journal_title：Journal of clinical microbiology

authors： Zhang L,Xiao M,Wang H,Gao R,Fan X,Brown M,Gray TJ,Kong F,Xu YC

更新日期：2014-02-01 00:00:00

abstract：:The human metapneumovirus (hMPV) was recently identified and linked to acute respiratory tract infections (ARTI). To assess the clinical importance of this virus in infants and children, we developed a rapid and efficient reverse transcription-PCR-based screening method for a large volume of samples and tested retrosp...

journal_title：Journal of clinical microbiology

authors： Chano F,Rousseau C,Laferrière C,Couillard M,Charest H

更新日期：2005-11-01 00:00:00

abstract：:The epidemiology of hospital infections due to Pseudomonas aeruginosa was investigated by pyocin typing. The typing method, which determined the pyocin activity of clinical isolates of P. aeruginosa on 27 indicator strains, was 43.7% reproducible, but elimination of 9 indicator strains doubled the reproducibility and ...

journal_title：Journal of clinical microbiology

authors： Bruun FN,McGarrity GJ,Blakemore WS,Coriell LL

更新日期：1976-03-01 00:00:00

abstract：:We report the first case of spontaneous intrapartum Atopobium vaginae bacteremia identified by 16S rRNA gene sequencing. The bacterium was misidentified by RapID ANA II, API Rapid ID 32A, and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The likely source of bacteremia wa...

journal_title：Journal of clinical microbiology

authors： Chan JF,Lau SK,Curreem SO,To KK,Leung SS,Cheng VC,Yuen KY,Woo PC

更新日期：2012-07-01 00:00:00

abstract：:The technique of random amplified polymorphic DNA (RAPD) was adapted and optimized to study Haemophilus ducreyi isolates. A panel of 43 strains isolated from chancroid patients from different countries in Africa, Europe, North America, and Asia were characterized. The strains were also studied with respect to lipoolig...

journal_title：Journal of clinical microbiology

authors： Mbwana J,Bölin I,Lyamuya E,Mhalu F,Lagergård T

更新日期：2006-01-01 00:00:00

abstract：:We evaluated the Xpert MRSA/SA SSTI real-time PCR assay (Cepheid, Sunnyvale, CA) directly on perioperative bone and joint samples. The sensitivity and specificity for detection of methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), and methicillin-resistant coagula...

journal_title：Journal of clinical microbiology

authors： Dubouix-Bourandy A,de Ladoucette A,Pietri V,Mehdi N,Benzaquen D,Guinand R,Gandois JM

更新日期：2011-12-01 00:00:00

abstract：:Syrian hamsters were shown to be susceptible to infection by the Lyme disease spirochete Borrelia burgdorferi. Although these spirochetes did not cause any outward signs of illness in these animals, they did cause a generalized infection. Spirochetemia was present during the first 6 days of infection. At 14 days posti...

journal_title：Journal of clinical microbiology

authors： Johnson RC,Marek N,Kodner C

更新日期：1984-12-01 00:00:00

abstract：:A total of 4,409 stool specimens from infants less than 5 years of age seeking treatment for diarrhea in Matlab, Bangladesh, were tested for the presence of adenoviruses by using an enzyme immunoassay (EIA). EIA-positive stool samples were serotyped with monoclonal antibodies specific for adenovirus type 40 (Ad40) and...

journal_title：Journal of clinical microbiology

authors： Jarecki-Khan K,Tzipori SR,Unicomb LE

更新日期：1993-03-01 00:00:00

abstract：:Mycobacterium avium subsp. paratuberculosis causes Johne's disease, a systemic infection and chronic inflammation of the intestine that affects many species, including primates. Infection is widespread in livestock, and human populations are exposed. Johne's disease is associated with immune dysregulation, with involv...

journal_title：Journal of clinical microbiology

authors： Scanu AM,Bull TJ,Cannas S,Sanderson JD,Sechi LA,Dettori G,Zanetti S,Hermon-Taylor J

更新日期：2007-12-01 00:00:00

abstract：:Campylobacter jejuni isolates from stool samples of patients with domestically acquired sporadic infections and from chicken from retail shops were studied during seasonal peaks from June to September over a 3-year period from 1996 to 1998. A large number of pulsed-field gel electrophoresis (PFGE) genotypes (a combine...

journal_title：Journal of clinical microbiology

authors： Hänninen ML,Perko-Mäkelä P,Pitkälä A,Rautelin H

更新日期：2000-05-01 00:00:00

abstract：:A Campylobacter-like organism was isolated from the ilea of normal hamsters. The organism was isolated from an ileal homogenate which was passed through a filter (0.65-micron pore size) and cultured on blood-agar plates in a microaerophilic atmosphere at 37 degrees C. Pinpoint translucent colonies were first observed ...

journal_title：Journal of clinical microbiology

authors： Stills HF Jr,Hook RR Jr,Kinden DA

更新日期：1989-11-01 00:00:00

abstract：:This study investigated a rare area of endemicity with a high prevalence of both hepatitis B virus (HBV) and hepatitis C virus (HCV) infections, in Putian County, China. Among 1,050 subjects, the overall prevalence of HBsAg seropositivity was 15.8%, and that of anti-HCV seropositivity was 28.9%. Intrafamilial viral tr...

journal_title：Journal of clinical microbiology

authors： Lu WP,Lin GX,Shi S,Dong JH

更新日期：2012-06-01 00:00:00

abstract：:The detection of immunoglobulin M (IgM) antibodies to cytomegalovirus-induced late antigens by an indirect immunofluorescence assay was improved by the use of monoclonal antibodies to human IgM. Nonspecific background fluorescence was absent, facilitating the reading of the slides and the detection of a specific fluor...

journal_title：Journal of clinical microbiology

authors： Zerbini M,Musiani M,Gentilomi G,La Placa M

更新日期：1986-07-01 00:00:00

abstract：:The mainstay of laboratory diagnosis for Lyme disease is two-tiered serological testing, in which a reactive first-tier enzyme-linked immunosorbent assay (ELISA) or an immunofluorescence assay is supplemented by separate IgM and IgG immunoblots. Recent data suggest that the C6 ELISA can be substituted for immunoblots ...

journal_title：Journal of clinical microbiology

authors： Wormser GP,Levin A,Soman S,Adenikinju O,Longo MV,Branda JA

更新日期：2013-12-01 00:00:00

abstract：:Fourth-generation human immunodeficiency virus (HIV) screening immunoassays reduce the diagnostic window between infection and diagnosis by the inclusion of HIV p24 antigen detection together with HIV antibody detection in the same test. We compared third- and fourth-generation HIV immunoassays and a dedicated HIV p24...

journal_title：Journal of clinical microbiology

authors： Speers D,Phillips P,Dyer J

更新日期：2005-10-01 00:00:00

abstract：:A direct immunofluorescence assay (DFA) with a monoclonal antibody from Ortho Diagnostic Systems was compared with conventional cell culture for the rapid detection of varicella-zoster virus (VZV) in 140 dermal lesions from 133 patients. A total of 79 (56%) specimens were positive for VZV: 40 (51%) by DFA alone, 2 (3%...

journal_title：Journal of clinical microbiology

authors： Coffin SE,Hodinka RL

更新日期：1995-10-01 00:00:00

abstract：:Quantitative real-time PCR for the detection of respiratory syncytial virus (RSV) RNA is increasingly used to study the causal role of RSV in lower airway disease. The objective of our study was to evaluate variations in RSV RNA loads at different steps in the RNA quantification process: (i) variation in RSV RNA load ...

journal_title：Journal of clinical microbiology

authors： van de Pol AC,Wolfs TF,van Loon AM,Tacke CE,Viveen MC,Jansen NJ,Kimpen JL,Rossen JW,Coenjaerts FE

更新日期：2010-10-01 00:00:00

abstract：:The Roche PGMY primer-based research prototype line blot assay (PGMY-LB) is a convenient tool in epidemiological studies for the detection and typing of human papillomavirus (HPV) DNA. This assay has been optimized and is being commercialized as the Linear Array HPV genotyping test (LA-HPV). We assessed the agreement ...

journal_title：Journal of clinical microbiology

authors： Coutlée F,Rouleau D,Petignat P,Ghattas G,Kornegay JR,Schlag P,Boyle S,Hankins C,Vézina S,Coté P,Macleod J,Voyer H,Forest P,Walmsley S,Canadian Women's HIV study Group.,Franco E

更新日期：2006-06-01 00:00:00

abstract：:Many parts of the Salmonella rfb gene clusters which are responsible for biosynthesis of the oligosaccharide-repeating units of the O-antigenic lipopolysaccharide have recently been cloned and sequenced. On the basis of this knowledge, three sets of nucleotide primers were selected to target defined regions of the abe...

journal_title：Journal of clinical microbiology

authors： Luk JM,Kongmuang U,Reeves PR,Lindberg AA

更新日期：1993-08-01 00:00:00

abstract：:Seventy-eight blood cultures with a Gram stain result of Gram-positive cocci in pairs and/or chains were evaluated with the Nanosphere Verigene Gram-positive blood culture (BC-GP) assay. The overall concordance of the assay with culture was 89.7% (70/78 cultures), allowing for the development of a targeted treatment a...

journal_title：Journal of clinical microbiology

authors： Alby K,Daniels LM,Weber DJ,Miller MB

更新日期：2013-11-01 00:00:00

abstract：:A rapid and sensitive PCR assay for the detection of Candida albicans DNA in serum was established. DNA from human serum samples was purified using the QIAamp blood kit, which proved to be a fast and simple method for isolating minute amounts of Candida DNA from clinical specimens for diagnosis of invasive candidiasis...

journal_title：Journal of clinical microbiology

authors： Wahyuningsih R,Freisleben HJ,Sonntag HG,Schnitzler P

更新日期：2000-08-01 00:00:00

abstract：:This study demonstrates the dynamics in the epidemiology of hepatitis C virus subtypes. Subtypes 3a and 4a have become increasingly prevalent in patients where an infection within recent years can be assumed. Evidence is presented that the subtypes observed among younger patients can spread rapidly and lead to signifi...

journal_title：Journal of clinical microbiology

authors： Schröter M,Zöllner B,Schäfer P,Reimer A,Müller M,Laufs R,Feucht HH

更新日期：2002-05-01 00:00:00

abstract：:Results of a 2003 survey carried out in Italy to evaluate the prevalence of extended-spectrum beta-lactamase (ESBL)-producing enterobacteria are presented. Eleven Italian Microbiology Laboratories investigated 9,076 consecutive nonreplicate isolates (inpatients, 6,850; outpatients, 2,226). ESBL screening was performed...

journal_title：Journal of clinical microbiology

authors： Luzzaro F,Mezzatesta M,Mugnaioli C,Perilli M,Stefani S,Amicosante G,Rossolini GM,Toniolo A

更新日期：2006-05-01 00:00:00

abstract：:The present study describes a method for amplifying DNA in Actinobacillus actinomycetemcomitans by using short, synthetic oligonucleotides of random sequence as primers in the polymerase chain reaction. Genomic DNA from each of 20 human isolates of A. actinomycetemcomitans was successfully amplified in a thermal cycle...

journal_title：Journal of clinical microbiology

authors： Preus HR,Haraszthy VI,Zambon JJ,Genco RJ

更新日期：1993-10-01 00:00:00

abstract：:Comparative sequence analysis was performed upon Bacillus anthracis and its closest relatives, B. cereus and B. thuringiensis. Portions of rpoB DNA from 10 strains of B. anthracis, 16 of B. cereus, 10 of B. thuringiensis, 1 of B. mycoides, and 1 of B. megaterium were amplified and sequenced. The determined rpoB sequen...

journal_title：Journal of clinical microbiology

authors： Ko KS,Kim JM,Kim JW,Jung BY,Kim W,Kim IJ,Kook YH

更新日期：2003-07-01 00:00:00

abstract：:Using data from an ongoing Swedish intervention project, the observed durations of nasopharyngeal carriage of penicillin-nonsusceptible Streptococcus pneumoniae (PNSP) (MIC of penicillin G of >or=0.5 microg/ml) stratified by both pneumococcal serogroup and age of the carrier were compared. The means and 95% confidence...

journal_title：Journal of clinical microbiology

authors： Högberg L,Geli P,Ringberg H,Melander E,Lipsitch M,Ekdahl K

更新日期：2007-03-01 00:00:00

abstract：:Because strains of Staphylococcus aureus that are resistant to penicillinase-resistant penicillins may be difficult to detect in the clinical laboratory, a variety of changes in methodology have been suggested to increase their detection. In 1984, the West Los Angeles Veterans Administration Medical Center experienced...

journal_title：Journal of clinical microbiology

authors： Mulligan ME,Citron DM,Kwok RY,Wheelock JP,Farrohi FK,Hindler JA,Johnston L

更新日期：1987-05-01 00:00:00