Abstract:
:The genetic and phylogenetic characteristics of human enterovirus 71 (EV71) and coxsackievirus A16 (CA16) sampled from children with hand, foot, and mouth disease in Shenzhen, People's Republic of China, over a 6-year period (1999 to 2004) were examined with reverse transcription-PCR and DNA sequencing. Out of 147 stool specimens, 60 showed positive signals when screened with EV71- and CA16-specific primers. EV71 was identified in 19 specimens, and CA16 was identified in 41 specimens; coinfection by EV71 and CA16 was not observed. Phylogenetic analysis of all EV71 strains isolated from the mainland Chinese samples established C4 as the predominant genotype. Only one other known strain (3254-TAI-98; AF286531), isolated in Taiwan in 1998, was identified as belonging to genotype C4. Phylogenetic analysis of CA16 strains allowed us to identify three new genetic lineages (A, B, and C), with lineage C recently predominating in Asian countries, such as the People's Republic of China, Malaysia, and Japan. These new observations indicate that CA16 circulating in the People's Republic of China is genetically diverse, and additional surveillance is warranted.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Li L,He Y,Yang H,Zhu J,Xu X,Dong J,Zhu Y,Jin Qdoi
10.1128/JCM.43.8.3835-3839.2005subject
Has Abstractpub_date
2005-08-01 00:00:00pages
3835-9issue
8eissn
0095-1137issn
1098-660Xpii
43/8/3835journal_volume
43pub_type
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pub_type: 临床试验,杂志文章
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