PCR detection of North American and Central African isolates of epizootic hemorrhagic disease virus (EHDV) based on genome segment 10 of EHDV serotype 1.

abstract：:Bacillus anthracis and Bacillus cereus are closely related pathogenic organisms that are difficult to differentiate phenotypically or genotypically. It is well known that vegetative and spore forms of bacilli are quite distinct both morphologically and chemically, but spore-specific chemical markers allowing these spe...

journal_title：Journal of clinical microbiology

authors： Fox A,Black GE,Fox K,Rostovtseva S

更新日期：1993-04-01 00:00:00

abstract：:Eight genotypes (A to H) and nine subtypes (adw2, adw4, ayw1, ayw2, ayw3, ayw4, adrq+, adrq-, and ayr) of hepatitis B virus (HBV) have been identified worldwide. They appear to be associated with geographical distribution, virological characteristics, and possibly clinical outcomes. We performed sequence analysis of p...

journal_title：Journal of clinical microbiology

authors： Lusida MI,Nugrahaputra VE,Soetjipto,Handajani R,Nagano-Fujii M,Sasayama M,Utsumi T,Hotta H

更新日期：2008-07-01 00:00:00

abstract：:We used the slide agglutination test to determine the serologic relationships of 22 Legionella spp. representing 33 serogroups. Antisera prepared against 14 of the Legionella spp. contained cross-reactive antibodies (1+ or greater) at their working dilutions. Numerous cross-reactions were observed for the blue-white f...

journal_title：Journal of clinical microbiology

authors： Thacker WL,Plikaytis BB,Wilkinson HW

更新日期：1985-05-01 00:00:00

abstract：:To evaluate the risk of a nosocomial spread of Pneumocystis carinii f. sp. hominis (P. carinii hominis), air filter samples from rooms of P. carinii pneumonia (PCP) patients, adjacent corridors, and other hospital environments have been investigated for the presence of P. carinii hominis. Amplified DNA from air filter...

journal_title：Journal of clinical microbiology

authors： Olsson M,Lidman C,Latouche S,Björkman A,Roux P,Linder E,Wahlgren M

更新日期：1998-06-01 00:00:00

abstract：:The DNA fingerprint profiles and somatic serotypes of 71 Pasteurella multocida capsule serogroup B isolates, 13 capsule serogroup E isolates, and 16 somatic reference serotype strains were compared. Each of the 16 reference somatic serotypes had a unique DNA fingerprint profile with the HhaI restriction endonuclease. ...

journal_title：Journal of clinical microbiology

authors： Wilson MA,Rimler RB,Hoffman LJ

更新日期：1992-06-01 00:00:00

abstract：:The Centers for Disease Control and Prevention recommended confirming positive screening tests for Chlamydia trachomatis when positive predictive values are <90%. It is accepted that less sensitive tests (i.e., culture and immunoassays) should not be used to confirm the results of more sensitive nucleic acid amplifica...

journal_title：Journal of clinical microbiology

authors： Schachter J,Hook EW,Martin DH,Willis D,Fine P,Fuller D,Jordan J,Janda WM,Chernesky M

更新日期：2005-03-01 00:00:00

abstract：:A new method for determining the MICs for Aspergillus species with slant-shaking incubation is described. The method was rapid (24 h), produced sharp visual end points, and showed high reproducibility. Additionally, MICs measured by this method correlated well with, as well as were two- to sixfold lower than, those by...

journal_title：Journal of clinical microbiology

authors： Yamada H,Kohno S,Maesaki S,Koga H,Kaku M,Hara K,Tanaka H

更新日期：1993-04-01 00:00:00

abstract：:Sera from goats suffering from caprine arthritis-encephalitis contained antibodies to virus proteins of 15, 17, 28, 40, and 130 kilodaltons in immunoblots of maedi-visna virus. We propose to use immunoblotting as a validation test for enzyme-linked immunosorbent assay and demonstrate that the specificity of indirect e...

journal_title：Journal of clinical microbiology

authors： Zanoni R,Krieg A,Peterhans E

更新日期：1989-03-01 00:00:00

abstract：:Nonfermenting coryneform bacteria identified as Brevibacterium spp. were isolated from routine clinical specimens. Four strains were derived from peritoneal fluid and has presumably been involved in the pathogenesis of continuous ambulatory peritoneal dialysis peritonitis. Another five isolates most probably represent...

journal_title：Journal of clinical microbiology

authors： Gruner E,Pfyffer GE,von Graevenitz A

更新日期：1993-06-01 00:00:00

abstract：:Saliva from subjects with amebic liver abscess (ALA), acute amebic colitis, asymptomatic infection with Entamoeba histolytica or Entamoeba dispar, and uninfected controls was tested by enzyme-linked immunosorbent assay (ELISA) for the presence of E. histolytica galactose-inhibitable lectin antigen and salivary immunog...

journal_title：Journal of clinical microbiology

authors： Abd-Alla MD,Jackson TF,Reddy S,Ravdin JI

更新日期：2000-06-01 00:00:00

abstract：:Based on cultural and biochemical tests, a total of 84 strains (72 clinical and 12 environmental isolates from the Caribbean Isles, Europe, and the Indian subcontinent) were identified as members of the Mycobacterium avium complex (MAC). They were further characterized with MAC, M. avium, and M. intracellulare probes ...

journal_title：Journal of clinical microbiology

authors： Devallois A,Picardeau M,Paramasivan CN,Vincent V,Rastogi N

更新日期：1997-11-01 00:00:00

abstract：:Toxoplasmic lymphadenopathy (TL) is the most common clinical manifestation of acute acquired toxoplasma infection in normal individuals. The diagnosis is established by serologic methods and lymph node biopsy. Recently, tests for avidity of toxoplasma immunoglobulin G (IgG) antibodies have been introduced to help disc...

journal_title：Journal of clinical microbiology

authors： Montoya JG,Huffman HB,Remington JS

更新日期：2004-10-01 00:00:00

abstract：:A total of 2,559 routine clinical specimens were cultured for mycobacteria by using BACTEC Middlebrook 7H12 medium (BACTEC), Lowenstein-Jensen slants (LJ), and Mycobactosel selective Middlebrook 7H11 slants (M7H11). Thirty-three isolates (1.3%) of M. avium complex and 82 isolates (3.2%) of M. tuberculosis were recover...

journal_title：Journal of clinical microbiology

authors： Kirihara JM,Hillier SL,Coyle MB

更新日期：1985-11-01 00:00:00

abstract：:The complement fixation (CF) test is the current reference serologic test for the diagnosis of Mycoplasma pneumoniae infection. However, it is reported to be insensitive and nonspecific, and it is labor intensive. To determine if a faster and more sensitive diagnosis of M. pneumoniae could be obtained, we examined 50 ...

journal_title：Journal of clinical microbiology

authors： Thacker WL,Talkington DF

更新日期：1995-05-01 00:00:00

abstract：:A biotyping scheme using improved media and methods for the detection of hippurate hydrolysis, rapid H2S production, and DNA hydrolysis was applied to 1,826 cultures of Campylobacter jejuni, Campylobacter coli and "Campylobacter laridis" isolates from human and nonhuman sources. Four biotypes were identified among C. ...

journal_title：Journal of clinical microbiology

authors： Lior H

更新日期：1984-10-01 00:00:00

abstract：:The currently available diagnostics for Clostridium difficile infection (CDI) have major limitations. Despite mounting evidence that toxin detection is paramount for diagnosis, conventional toxin immunoassays are insufficiently sensitive and cytotoxicity assays too complex; assays that detect toxigenic organisms (toxi...

journal_title：Journal of clinical microbiology

authors： Song L,Zhao M,Duffy DC,Hansen J,Shields K,Wungjiranirun M,Chen X,Xu H,Leffler DA,Sambol SP,Gerding DN,Kelly CP,Pollock NR

更新日期：2015-10-01 00:00:00

abstract：:The present investigation focused on genetic diversity and drug resistance of 101 Mycobacterium tuberculosis strains isolated between July 2003 and February 2005 in the Okinawa prefecture, Ryukyu Islands, Japan. A high rate of clustering (87%, eight clusters, 2 to 69 strains/cluster) was observed upon spoligotyping; m...

journal_title：Journal of clinical microbiology

authors： Millet J,Miyagi-Shiohira C,Yamane N,Sola C,Rastogi N

更新日期：2007-11-01 00:00:00

abstract：:The taxonomic relationship of Rhinosporidium seeberi with other organisms remained controversial for over a century. Recently, molecular studies have shown R. seeberi to be a protistal microbe in the newly described class Mesomycetozoea at the animal-fungal boundary. Phylogenetic analyses of R. seeberi using 18S small...

journal_title：Journal of clinical microbiology

authors： Silva V,Pereira CN,Ajello L,Mendoza L

更新日期：2005-04-01 00:00:00

abstract：:Human bocavirus (HBoV) was discovered in 2005 and is associated with respiratory tract symptoms in young children. Three additional members of the genus Bocavirus, HBoV2, -3, and -4, were discovered recently from fecal specimens, and early results indicate an association between HBoV2 and gastrointestinal disease. In ...

journal_title：Journal of clinical microbiology

authors： Kantola K,Sadeghi M,Antikainen J,Kirveskari J,Delwart E,Hedman K,Söderlund-Venermo M

更新日期：2010-11-01 00:00:00

abstract：:Of 1,510 sputum samples examined from 1,016 patients with bronchopulmonary disorders, Nocardia asteroides was isolated from 67 samples by paraffin baiting, as compared with only 30 isolations by the conventional technique of culturing on Sabouraud dextrose agar. This higher efficacy of the paraffin bait technique was ...

journal_title：Journal of clinical microbiology

authors： Singh M,Sandhu RS,Randhawa HS

更新日期：1987-01-01 00:00:00

abstract：:Nearly 400,000 people worldwide are known to have been infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) beginning in December 2019. The virus has now spread to over 168 countries including the United States, where the first cluster of cases was observed in the Seattle metropolitan area in Was...

journal_title：Journal of clinical microbiology

authors： Nalla AK,Casto AM,Huang MW,Perchetti GA,Sampoleo R,Shrestha L,Wei Y,Zhu H,Jerome KR,Greninger AL

更新日期：2020-05-26 00:00:00

abstract：:Traveler's diarrhea (TD) is the most common infectious illness acquired by visitors to developing nations. The purpose of this study was to utilize molecular diagnostic techniques to determine the prevalence of norovirus (NoV) in TD occurring among visitors from the United States to Guatemala and Mexico. Stool samples...

journal_title：Journal of clinical microbiology

authors： Chapin AR,Carpenter CM,Dudley WC,Gibson LC,Pratdesaba R,Torres O,Sanchez D,Belkind-Gerson J,Nyquist I,Kärnell A,Gustafsson B,Halpern JL,Bourgeois AL,Schwab KJ

更新日期：2005-03-01 00:00:00

abstract：:Malignant catarrhal fever (MCF) was diagnosed by clinical signs and lesions in five out of six white-tailed deer (Odocoileus virginianus) in a North American zoo. The clinical signs and histopathological lesions in these deer were typical of MCF. Antibody to an epitope conserved among the MCF viruses was detected in t...

journal_title：Journal of clinical microbiology

authors： Li H,Dyer N,Keller J,Crawford TB

更新日期：2000-04-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been found to be an accurate, rapid, and inexpensive method for the identification of bacteria and yeasts. Previous evaluations have compared the accuracy, time to identification, and costs of the MALDI-TOF MS method agains...

journal_title：Journal of clinical microbiology

authors： Tan KE,Ellis BC,Lee R,Stamper PD,Zhang SX,Carroll KC

更新日期：2012-10-01 00:00:00

abstract：:In this study, 5-iodo-2'-deoxyuridine-, cytochalasin B-, and cycloheximide-treated and untreated McCoy cells were inoculated with representative strains of Chlamydia trachomatis serotypes, as well as with clinical specimens from a pediatric population, and the quality and quantity of the cytoplasmic inclusions were ex...

journal_title：Journal of clinical microbiology

authors： La Scolea LJ Jr,Keddell JE

更新日期：1981-04-01 00:00:00

abstract：:In this work, we describe the assembly of a synthetic gene coding for several antigenic determinants found in different Leishmania infantum antigens. Selected epitopes were derived from the ribosomal proteins LiP2a, LiP2b, and LiP0 and from the histone H2A. The resulting gene was overexpressed in Escherichia coli eith...

journal_title：Journal of clinical microbiology

authors： Soto M,Requena JM,Quijada L,Alonso C

更新日期：1998-01-01 00:00:00

abstract：:Sequence analysis of the internal transcribed spacer (ITS) region was employed as the gold standard method for yeast identification in the China Hospital Invasive Fungal Surveillance Net (CHIF-NET). It has subsequently been found that matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-...

journal_title：Journal of clinical microbiology

authors： Zhang L,Xiao M,Wang H,Gao R,Fan X,Brown M,Gray TJ,Kong F,Xu YC

更新日期：2014-02-01 00:00:00

abstract：:High-risk human papillomavirus (hrHPV) testing is now being introduced as a potential primary screening test for improved detection of cervical precancer and cancer. Current U.S. Food and Drug Administration-approved tests are batch tests that take several hours to complete. A rapid, non-batch test might permit point-...

journal_title：Journal of clinical microbiology

authors： Einstein MH,Smith KM,Davis TE,Schmeler KM,Ferris DG,Savage AH,Gray JE,Stoler MH,Wright TC Jr,Ferenczy A,Castle PE

更新日期：2014-06-01 00:00:00

abstract：:Molecular typing of 246 Staphylococcus aureus isolates from unselected patients in Thailand showed that 10 (4.1%) were actually Staphylococcus argenteus. Contrary to the suggestion that S. argenteus is less virulent than S. aureus, we demonstrated comparable rates of morbidity, death, and health care-associated infect...

journal_title：Journal of clinical microbiology

authors： Thaipadungpanit J,Amornchai P,Nickerson EK,Wongsuvan G,Wuthiekanun V,Limmathurotsakul D,Peacock SJ

更新日期：2015-03-01 00:00:00

abstract：:Using data from an ongoing Swedish intervention project, the observed durations of nasopharyngeal carriage of penicillin-nonsusceptible Streptococcus pneumoniae (PNSP) (MIC of penicillin G of >or=0.5 microg/ml) stratified by both pneumococcal serogroup and age of the carrier were compared. The means and 95% confidence...

journal_title：Journal of clinical microbiology

authors： Högberg L,Geli P,Ringberg H,Melander E,Lipsitch M,Ekdahl K

更新日期：2007-03-01 00:00:00