Real-time quantitative PCR detection of four human bocaviruses.

abstract：:A rapid and sensitive PCR assay for the detection of Candida albicans DNA in serum was established. DNA from human serum samples was purified using the QIAamp blood kit, which proved to be a fast and simple method for isolating minute amounts of Candida DNA from clinical specimens for diagnosis of invasive candidiasis...

journal_title：Journal of clinical microbiology

authors： Wahyuningsih R,Freisleben HJ,Sonntag HG,Schnitzler P

更新日期：2000-08-01 00:00:00

abstract：:The sensitivities of MRC-5 and mink lung (ML) cells in centrifugation culture were compared simultaneously for the detection of cytomegalovirus (CMV) IE antigen (immediate-early antigen) from clinical specimens. Of 413 samples assayed, 51 (12%) were positive for CMV by both centrifugation and standard cell culture. At...

journal_title：Journal of clinical microbiology

authors： Gleaves CA,Hursh DA,Meyers JD

更新日期：1992-04-01 00:00:00

abstract：:The rapid identification of blood culture isolates and antimicrobial susceptibility test (AST) results play critical roles for the optimal treatment of patients with bloodstream infections. Whereas others have looked at the time to detection in automated culture systems, we examined the overall time from specimen coll...

journal_title：Journal of clinical microbiology

authors： Tabak YP,Vankeepuram L,Ye G,Jeffers K,Gupta V,Murray PR

更新日期：2018-11-27 00:00:00

abstract：:Diagnosis of invasive aspergillosis (IA) is still a major problem in routine clinical practice. Early diagnosis is essential for a good patient prognosis. PCR is a highly sensitive method for the detection of nucleic acids and could play an important role in improving the diagnosis of fungal infections. Therefore, a n...

journal_title：Journal of clinical microbiology

authors： Springer J,Schloßnagel H,Heinz W,Doedt T,Soeller R,Einsele H,Loeffler J

更新日期：2012-08-01 00:00:00

abstract：:Infections with human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2, respectively) are zoonotic infections. In Africa, the potential exists for additional cross-species transmissions from at least 33 different species of simian immunodeficiency virus (SIV)-infected nonhuman primates (NHPs) through hunting and ...

journal_title：Journal of clinical microbiology

authors： Ndongmo CB,Switzer WM,Pau CP,Zeh C,Schaefer A,Pieniazek D,Folks TM,Kalish ML

更新日期：2004-11-01 00:00:00

abstract：:A Campylobacter-like organism was isolated from the ilea of normal hamsters. The organism was isolated from an ileal homogenate which was passed through a filter (0.65-micron pore size) and cultured on blood-agar plates in a microaerophilic atmosphere at 37 degrees C. Pinpoint translucent colonies were first observed ...

journal_title：Journal of clinical microbiology

authors： Stills HF Jr,Hook RR Jr,Kinden DA

更新日期：1989-11-01 00:00:00

abstract：:Antibiotic-associated hemorrhagic colitis (AAHC) is associated with Klebsiella oxytoca. This study analyzed whether cytotoxic properties are linked to specific subtypes of K. oxytoca. Klebsiella isolates from stools of AAHC patients, healthy carriers, and diarrhea patients as well as from infections of other organs we...

journal_title：Journal of clinical microbiology

authors： Joainig MM,Gorkiewicz G,Leitner E,Weberhofer P,Zollner-Schwetz I,Lippe I,Feierl G,Krause R,Hinterleitner T,Zechner EL,Högenauer C

更新日期：2010-03-01 00:00:00

abstract：:Clostridium difficile is the leading cause of antibiotic-associated diarrhea and pseudomembranous colitis, which have significant morbidity and mortality. Accurate and timely diagnosis is critical. Repeat enzyme immunoassay testing for C. difficile toxin has been recommended because of <100% sensitivity. All C. diffic...

journal_title：Journal of clinical microbiology

authors： Cardona DM,Rand KH

更新日期：2008-11-01 00:00:00

abstract：:PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B. cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus ...

journal_title：Journal of clinical microbiology

authors： Spilker T,Baldwin A,Bumford A,Dowson CG,Mahenthiralingam E,LiPuma JJ

更新日期：2009-08-01 00:00:00

abstract：:We standardized and assessed the performance of an in-house microtiter assay for determining the susceptibilities of Mycobacterium tuberculosis clinical isolates to isoniazid based on mycobacteriophage amplification technology. Seventy isolates (43 resistant and 27 sensitive according to the BACTEC 460 radiometric met...

journal_title：Journal of clinical microbiology

authors： Galí N,Domínguez J,Blanco S,Prat C,Alcaide F,Coll P,Ausina V

更新日期：2006-01-01 00:00:00

abstract：:The fungus Fusarium sacchari was isolated repeatedly from the blood of an immunosuppressed host. The infection was treated successfully with a small dose of amphotericin B. The strain was resistant to this antifungal in vitro. MICs and minimum fungicidal concentrations of six antifungals for the clinical isolate are p...

journal_title：Journal of clinical microbiology

authors： Guarro J,Nucci M,Akiti T,Gené J,Barreiro MD,Gonçalves RT

更新日期：2000-01-01 00:00:00

abstract：:Ehrlichia risticii causes an acute infectious disease in horses called Potomac horse fever. To investigate the biological diversity of E. risticii organisms, nine E. risticii isolates derived from the peripheral blood monocytes of clinically sick horses in Ohio and Kentucky during the summers of 1991 and 1993 were com...

journal_title：Journal of clinical microbiology

authors： Chaichanasiriwithaya W,Rikihisa Y,Yamamoto S,Reed S,Crawford TB,Perryman LE,Palmer GH

更新日期：1994-12-01 00:00:00

abstract：:An automated enzymatic method was developed for the measurement of D-arabinitol in human serum. The assay is based on a novel, highly specific D-arabinitol dehydrogenase from Candida tropicalis. This enzyme catalyzes the oxidation of D-arabinitol to D-ribulose and the concomitant reduction of NAD+ to NADH. The NADH pr...

journal_title：Journal of clinical microbiology

authors： Switchenko AC,Miyada CG,Goodman TC,Walsh TJ,Wong B,Becker MJ,Ullman EF

更新日期：1994-01-01 00:00:00

abstract：:Bovine vaginal cytobrush specimens were analyzed for the presence of Chlamydia spp. by a high-sensitivity, high-specificity quantitative PCR. The 53% prevalence of low-level Chlamydia psittaci and C. pecorum genital infection detected in virgin heifers suggests predominantely extragenital transmission of Chlamydia in ...

journal_title：Journal of clinical microbiology

authors： DeGraves FJ,Gao D,Hehnen HR,Schlapp T,Kaltenboeck B

更新日期：2003-04-01 00:00:00

abstract：:The purpose of our study was the molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in 21 hospitals in the Czech Republic in the period 2000-2002 and comparison with previous results from 1996-1997. Strains were analyzed by pulsed-field gel electrophoresis (PFGE) of SmaI ...

journal_title：Journal of clinical microbiology

authors： Melter O,Aires de Sousa M,Urbásková P,Jakubů V,Zemlicková H,de Lencastre H

更新日期：2003-11-01 00:00:00

abstract：:The rate of false-positive hepatitis C virus enzyme immunoassay results was determined to be at least 10% among 1,814 reactive serum samples based on (i) negative results in an independent confirmation assay, (ii) negative PCR results, and (iii) no patients developing clinical or biochemical signs of hepatitis during ...

journal_title：Journal of clinical microbiology

authors： Schröter M,Feucht HH,Schäfer P,Zöllner B,Polywka S,Laufs R

更新日期：1999-01-01 00:00:00

abstract：:The Roche PGMY primer-based research prototype line blot assay (PGMY-LB) is a convenient tool in epidemiological studies for the detection and typing of human papillomavirus (HPV) DNA. This assay has been optimized and is being commercialized as the Linear Array HPV genotyping test (LA-HPV). We assessed the agreement ...

journal_title：Journal of clinical microbiology

authors： Coutlée F,Rouleau D,Petignat P,Ghattas G,Kornegay JR,Schlag P,Boyle S,Hankins C,Vézina S,Coté P,Macleod J,Voyer H,Forest P,Walmsley S,Canadian Women's HIV study Group.,Franco E

更新日期：2006-06-01 00:00:00

abstract：:In a recent genotypic survey of beta-lactam-resistant pneumococci recovered in different areas of United States during 1997, eight clonal types that each represented 3 to 40 isolates accounted for 134 of 144 isolates (G. Gherardi, C. Whitney, R. Facklam, and B. Beall, J. Infect. Dis. 181:216-229, 2000). We determined ...

journal_title：Journal of clinical microbiology

authors： Beall B,Gherardi G,Facklam RR,Hollingshead SK

更新日期：2000-10-01 00:00:00

abstract：:When the standard procedure for determining antibiotic susceptibility of bacteria is used, the results are delayed, especially for bacteria that grow slowly, such as Helicobacter pylori. Treatment for this bacterium may involve clarithromycin, a compound for which resistance has been associated with point mutations on...

journal_title：Journal of clinical microbiology

authors： Pina M,Occhialini A,Monteiro L,Doermann HP,Mégraud F

更新日期：1998-11-01 00:00:00

abstract：:Cross-reactivity between Legionella spp. and Capnocytophaga ochracea was noted by latex agglutination tests (Serobact Legionella; Disposable Products, Adelaide, Australia). Four of 11 (36%) C. ochracea isolates agglutinated with latex reagents designed to identify Legionella pneumophila serogroups. C. ochracea isolate...

journal_title：Journal of clinical microbiology

authors： Chen S,Hicks L,Yuen M,Mitchell D,Gilbert GL

更新日期：1994-12-01 00:00:00

abstract：:Two hundred and twelve Micrococcaceae isolates were obtained from 82 men with nongonococcal urethritis, 24 women with vaginitis, and 54 girls with vulvovaginitis. Identification and biotyping of these strains were carried out by using the simplified scheme of Kloos and Schleifer (W. E. Kloos and K. H. Schleifer, J. Cl...

journal_title：Journal of clinical microbiology

authors： Baldellon C,Mégraud F

更新日期：1985-03-01 00:00:00

abstract：:To determine that susceptibility of AMPLICOR Chlamydia trachomatis PCR to inhibitory factors possibly present in cervical specimens, we obtained cervical specimens from 200 gynecology patients attending our outpatient clinic. The prevalence of C. trachomatis infection was 4.1%, as determined by cell culture. All AMPLI...

journal_title：Journal of clinical microbiology

authors： Verkooyen RP,Luijendijk A,Huisman WM,Goessens WH,Kluytmans JA,van Rijsoort-Vos JH,Verbrugh HA

更新日期：1996-12-01 00:00:00

abstract：:Several studies have identified thymidine excess in susceptibility test media as the cause of spurious resistance of various bacteria to sulfamethoxazole-trimethoprim. We document the phenomenon in Salmonella typhi and Salmonella paratyphi-A and demonstrate its occurrence in 3 of 17 (18%) lots of Mueller-Hinton agars ...

journal_title：Journal of clinical microbiology

authors： Escamilla J,Kilpatrick ME,Ugarte HF

更新日期：1986-01-01 00:00:00

abstract：:Clostridium difficile is the most common cause of health care-associated diarrhea. Accurate and rapid diagnosis is essential to improve patient outcome and prevent disease spread. We compared our two-step diagnostic algorithm, an enzyme immunoassay for glutamate dehydrogenase (GDH) followed by the cytotoxin neutraliza...

journal_title：Journal of clinical microbiology

authors： Babady NE,Stiles J,Ruggiero P,Khosa P,Huang D,Shuptar S,Kamboj M,Kiehn TE

更新日期：2010-12-01 00:00:00

abstract：:A simple enzyme immunoassay has been developed to allow the quantitation of the human response to immunization with pneumococcal polysaccharide. The assay uses the 14-valent vaccine (Pneumovax) as a convenient antigen to adsorb to the solid-phase microdilution plate wells and commercially available isotype-specific an...

journal_title：Journal of clinical microbiology

authors： Shyamala GN,Roberton DM,Hosking CS

更新日期：1988-08-01 00:00:00

abstract：:Bacillus anthracis spores have been used as a biological weapon in the United States. We wanted to develop a safe, rapid method of sample preparation that provided safe DNA for the detection of spores in environmental and clinical specimens. Our method reproducibly detects B. anthracis in samples containing <10 spores...

journal_title：Journal of clinical microbiology

authors： Luna VA,King D,Davis C,Rycerz T,Ewert M,Cannons A,Amuso P,Cattani J

更新日期：2003-03-01 00:00:00

abstract：:A total of 375 Escherichia coli O157 strains were tested by colony hybridization with the eae probe from the central portion of the eaeA gene of the classical enteropathogenic E. coli strain E2348/69. They were also tested with a probe, eaeO157, from the C-terminal end of the eae gene homolog from a Vero cytotoxin (VT...

journal_title：Journal of clinical microbiology

authors： Willshaw GA,Scotland SM,Smith HR,Cheasty T,Thomas A,Rowe B

更新日期：1994-04-01 00:00:00

abstract：:Pulsed-field gel electrophoresis (PFGE) of XbaI-digested chromosomal DNA was performed on 133 strains of Salmonella enterica serovar Typhi obtained from Papua New Guinea, with the objective of assessing the temporal variation of these strains. Fifty-two strains that were isolated in 1992 and 1994 were of one phage typ...

journal_title：Journal of clinical microbiology

authors： Thong KL,Goh YL,Yasin RM,Lau MG,Passey M,Winston G,Yoannes M,Pang T,Reeder JC

更新日期：2002-11-01 00:00:00

abstract：:A survey for carriers of Streptococcus pneumoniae among 573 children was undertaken. Throat and nasopharyngeal cultures from 303 children disclosed pneumococci in 89 (29.4%) nasopharyngeal swabs but in only 8 (2.6%) throat specimens. Carriers of pneumococci were detected with frequencies of 84.4 and 79.9% on aerobical...

journal_title：Journal of clinical microbiology

authors： Robins-Browne RM,Kharsany AB,Ramsaroop UG

更新日期：1982-07-01 00:00:00

abstract：:We document for the first time an infectious but nonarthritogenic variant of Borrelia burgdorferi. Strain 25015, previously isolated from an Ixodes dammini larva collected in upstate New York, was infectious but failed to produce arthritis or carditis in laboratory rats and mice. By contrast, pathogenic strain N40 inv...

journal_title：Journal of clinical microbiology

authors： Anderson JF,Barthold SW,Magnarelli LA

更新日期：1990-12-01 00:00:00