Effects of fixation on polymerase chain reaction detection of Mycobacterium leprae.

abstract：:Of 45 influenza virus strains (43 type A and 2 type B) detected in conventional tube cell cultures (average time, 4 days), 25 (56%) were detected by immunofluorescence in the shell vial assay 24 h postinoculation. The specific fluorescence produced should allow this procedure to be readily adapted by laboratories with...

journal_title：Journal of clinical microbiology

authors： Espy MJ,Smith TF,Harmon MW,Kendal AP

更新日期：1986-10-01 00:00:00

abstract：:This study evaluates the performance of a PCR assay for the detection of Pneumocystis carinii from respiratory specimens that has been designed for use in the clinical microbiology laboratory. The test includes a simple method for nucleic acid extraction and amplification, a colorimetric probe hybridization technique ...

journal_title：Journal of clinical microbiology

authors： Caliendo AM,Hewitt PL,Allega JM,Keen A,Ruoff KL,Ferraro MJ

更新日期：1998-04-01 00:00:00

abstract：:We demonstrated that sodium gluconate was the factor causing false-positive galactomannan (GM) antigenemia of Plasma-Lyte hydration solution. Infusion of sodium gluconate-containing solution but not gluconate-free Plasma-Lyte resulted in positive serum GM antigenemia. Serum GM concentrations also correlated with the v...

journal_title：Journal of clinical microbiology

authors： Petraitiene R,Petraitis V,Witt JR 3rd,Durkin MM,Bacher JD,Wheat LJ,Walsh TJ

更新日期：2011-12-01 00:00:00

abstract：:We compared examination of unstained and Gram-stained smears as methods of evaluating sputum quality. Of 100 slides examined, 96 (96%) were graded identically. Each discrepancy between methods was near the cutoff for specimen acceptability. We concluded that examination of unstained smears can provide an accurate, cos...

journal_title：Journal of clinical microbiology

authors： Wetterau LM,Zeimis RT,Hollick GE

更新日期：1986-07-01 00:00:00

abstract：:A sensitive detection system specific for Legionella pneumophila in water was developed. This system is based on amplification of a chromosomal DNA sequence from L. pneumophila by the polymerase chain reaction, followed by detection of the amplified product by hybridization of a radiolabeled oligodeoxynucleotide. Afte...

journal_title：Journal of clinical microbiology

authors： Starnbach MN,Falkow S,Tompkins LS

更新日期：1989-06-01 00:00:00

abstract：:Rotaviruses contain a double-stranded ribonucleic acid genome consisting of 11 segments. Gel electrophoresis separates genome segments and allows identification of strain differences. This electrophoretic typing technique was applied to rotavirus specimens from 116 children and 72 newborn babies. Between 1973 and 1979...

journal_title：Journal of clinical microbiology

authors： Rodger SM,Bishop RF,Birch C,McLean B,Holmes IH

更新日期：1981-02-01 00:00:00

abstract：:Since the discovery of the Norwalk virus (NV) by immune electron microscopy (IEM) in 1972, serologic studies with this virus have relied on particle-positive fecal material from infected volunteers as the source of antigen because it has not been possible to propagate this virus in cell culture. However, the recent cl...

journal_title：Journal of clinical microbiology

authors： Green KY,Lew JF,Jiang X,Kapikian AZ,Estes MK

更新日期：1993-08-01 00:00:00

abstract：:Fungal infection of the thyroid is rare. Most reported cases have involved Aspergillus, Coccidioides, and Candida species in the setting of disseminated disease. Infection of the thyroid with Histoplasma capsulatum is rarely reported as part of disseminated disease, even in geographic areas where histoplasmosis is end...

journal_title：Journal of clinical microbiology

authors： Goldani LZ,Klock C,Diehl A,Monteiro AC,Maia AL

更新日期：2000-10-01 00:00:00

abstract：:The human herpesvirus 6 (HHV-6) early protein, p41, encoded by the U27 gene has been detected in oligodendrocytes of multiple sclerosis (MS) patients by using a monoclonal antibody (MAb to p41/38). We here report the antigenic epitope of HHV-6 p41 recognized by this MAb. First, we established that the MAb to p41/38 re...

journal_title：Journal of clinical microbiology

authors： Xu Y,Linde A,Dahl H,Winberg G

更新日期：2001-04-01 00:00:00

abstract：:Our results show that impairment of the gastrointestinal tracts in human immunodeficiency virus (HIV)-positive patients is present in the early phases of HIV disease. This impairment is associated with alterations in gut microbiota and intestinal inflammatory parameters. These findings support the hypothesis that alte...

journal_title：Journal of clinical microbiology

authors： Gori A,Tincati C,Rizzardini G,Torti C,Quirino T,Haarman M,Ben Amor K,van Schaik J,Vriesema A,Knol J,Marchetti G,Welling G,Clerici M

更新日期：2008-02-01 00:00:00

abstract：:The pandemic spread of Vibrio parahaemolyticus is an international public health issue. Because of the outbreak potential of the organism, it is critical to establish an internationally recognized molecular subtyping protocol for V. parahaemolyticus that is both rapid and robust as a means to monitor its further sprea...

journal_title：Journal of clinical microbiology

authors： Kam KM,Luey CK,Parsons MB,Cooper KL,Nair GB,Alam M,Islam MA,Cheung DT,Chu YW,Ramamurthy T,Pazhani GP,Bhattacharya SK,Watanabe H,Terajima J,Arakawa E,Ratchtrachenchai OA,Huttayananont S,Ribot EM,Gerner-Smidt P,Swamin

更新日期：2008-08-01 00:00:00

abstract：:From January 2011 to December 2013, a total of 262 samples, from 188 patients suspected of having syphilis were tested for the presence of treponemal DNA by PCR amplification of five chromosomal loci, including the polA (TP0105), tmpC (TP0319), TP0136, TP0548, and 23S rRNA genes. Altogether, 146 samples from 103 patie...

journal_title：Journal of clinical microbiology

authors： Grillová L,Pĕtrošová H,Mikalová L,Strnadel R,Dastychová E,Kuklová I,Kojanová M,Kreidlová M,Vaňousová D,Hercogová J,Procházka P,Zákoucká H,Krchňáková A,Vašků V,Šmajs D

更新日期：2014-10-01 00:00:00

abstract：:The NucliSENS easyMAG automated system was compared to the column-based Qiagen method for Epstein-Barr virus (EBV) or cytomegalovirus (CMV) DNA extraction from whole blood before viral load determination using the corresponding R-gene amplification kits. Both extraction techniques exhibited a total agreement of 81.3% ...

journal_title：Journal of clinical microbiology

authors： Pillet S,Bourlet T,Pozzetto B

更新日期：2009-11-01 00:00:00

abstract：:Quantitative fecal lactoferrin was measured in 112 patients tested for toxigenic Clostridium difficile using glutamate dehydrogenase (GDH) and toxin immunoassays combined with tcdB PCR. Lactoferrin levels were higher in the GDH-positive/toxin-positive group (79 μg/ml) than in the GDH-positive/toxin-negative/PCR-positi...

journal_title：Journal of clinical microbiology

authors： LaSala PR,Ekhmimi T,Hill AK,Farooqi I,Perrotta PL

更新日期：2013-01-01 00:00:00

abstract：:The BD Phoenix system was evaluated for species-level identification of yeasts (250 clinical isolates) and compared with the Vitek 2 system, using ribosomal internal transcribed spacer (ITS) sequence analysis as the gold standard. Considering only the species included in each system's database, 96.3% (236/245) and 91....

journal_title：Journal of clinical microbiology

authors： Posteraro B,Ruggeri A,De Carolis E,Torelli R,Vella A,De Maio F,Ricciardi W,Posteraro P,Sanguinetti M

更新日期：2013-11-01 00:00:00

abstract：:Mucosal and systemic immunologic recognition of cagA by Helicobacter pylori-infected individuals is associated with peptic ulcer disease; however, in the laboratory, expression of cagA is subject to artificial conditions which may not accurately reflect the conditions in host tissues. Gastric antral and body biopsy sp...

journal_title：Journal of clinical microbiology

authors： Peek RM Jr,Miller GG,Tham KT,Pérez-Pérez GI,Cover TL,Atherton JC,Dunn GD,Blaser MJ

更新日期：1995-01-01 00:00:00

abstract：:The utility of combined 16S rRNA (rrs) gene restriction fragment length polymorphism and plasmid profiles to differentiate between and within Campylobacter upsaliensis of human and canine origin was examined. Fourteen distinct rrs gene restriction fragment length polymorphs consisting of bands sized between 1.9 and 4....

journal_title：Journal of clinical microbiology

authors： Stanley J,Jones C,Burnens A,Owen RJ

更新日期：1994-07-01 00:00:00

abstract：:We surveyed 75 clinical laboratories to determine if National Committee for Clinical Laboratory Standards (NCCLS) were being used for the susceptibility testing of Haemophilus influenzae. Of the 66 laboratories that performed susceptibility testing, all claimed to follow current NCCLS guidelines. However, upon further...

journal_title：Journal of clinical microbiology

authors： Scriver SR,Hoban DJ,McGeer A,Moore TC,Walmsley SL,Low DE

更新日期：1994-08-01 00:00:00

abstract：:A newly developed enzyme immunoassay (EIA) was used to detect the presence of pneumococcal teichoic and lipoteichoic acids in cerebrospinal fluid (CSF) from patients with Streptococcus pneumoniae meningitis who were being treated with antibiotics. All initial CSF samples, which on culture grew S. pneumoniae, were posi...

journal_title：Journal of clinical microbiology

authors： Stuertz K,Merx I,Eiffert H,Schmutzhard E,Mäder M,Nau R

更新日期：1998-08-01 00:00:00

abstract：:We have developed a high-resolution melting (HRM) assay to scan for mutations in the rpoB, inhA, ahpC, and katG genes and/or promoter regions for the detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis. For assay development, 23 drug-resistant isolates of M. tuberculosis having 29 different mu...

journal_title：Journal of clinical microbiology

authors： Ong DC,Yam WC,Siu GK,Lee AS

更新日期：2010-04-01 00:00:00

abstract：:We recently developed a molecular typing system for Campylobacter jejuni and Campylobacter coli based on restriction fragment length polymorphism analysis of the flagellin gene,flaA (I.Nachamkin, K. Bohachick, and C.M. Patton, J. Clin. Microbiol. 31:1531-1536, 1993). We extended the typing system to 83 flagellin types...

journal_title：Journal of clinical microbiology

authors： Nachamkin I,Ung H,Patton CM

更新日期：1996-02-01 00:00:00

abstract：:Sepsis is a major cause of death worldwide among nonhospitalized people and hospitalized patients. A wide range of pathogens are involved, and the correct identification and correct antimicrobial therapy are critical to ensure optimal clinical outcomes. With the recent introduction of matrix-assisted laser desorption ...

journal_title：Journal of clinical microbiology

authors： Foster AG

更新日期：2013-11-01 00:00:00

abstract：:The genotypes of 63 isolates of Aspergillus fumigatus obtained from three hospitals in different geographical areas and of eight culture collection strains were determined by multilocus enzyme electrophoresis. Twelve of the 17 enzymatic loci studied were polymorphic, giving rise to 48 different electrophoretic types. ...

journal_title：Journal of clinical microbiology

authors： Rodriguez E,De Meeüs T,Mallie M,Renaud F,Symoens F,Mondon P,Piens MA,Lebeau B,Viviani MA,Grillot R,Nolard N,Chapuis F,Tortorano AM,Bastide JM

更新日期：1996-10-01 00:00:00

abstract：:We developed an Australian database for the identification of Aspergillus, Scedosporium, and Fusarium species (n = 28) by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). In a challenge against 117 isolates, species identification significantly improved when the in-house-bui...

journal_title：Journal of clinical microbiology

authors： Sleiman S,Halliday CL,Chapman B,Brown M,Nitschke J,Lau AF,Chen SC

更新日期：2016-08-01 00:00:00

abstract：:Clinical and experimental observations suggest that immune globulin may prevent otitis media (OM) in children. We performed experiments in chinchillas to test the hypothesis that human bacterial polysaccharide immune globulin (BPIG) might prevent OM caused by Streptococcus pneumoniae. Animals were given BPIG or saline...

journal_title：Journal of clinical microbiology

authors： Shurin PA,Giebink GS,Wegman DL,Ambrosino D,Rholl J,Overman M,Bauer T,Siber GR

更新日期：1988-04-01 00:00:00

abstract：:Sera from goats suffering from caprine arthritis-encephalitis contained antibodies to virus proteins of 15, 17, 28, 40, and 130 kilodaltons in immunoblots of maedi-visna virus. We propose to use immunoblotting as a validation test for enzyme-linked immunosorbent assay and demonstrate that the specificity of indirect e...

journal_title：Journal of clinical microbiology

authors： Zanoni R,Krieg A,Peterhans E

更新日期：1989-03-01 00:00:00

abstract：:Blastomyces dermatitidis and Histoplasma capsulatum are dimorphic fungi that often cause self-limited respiratory infections. However, they may also cause severe disseminated disease, depending on the level of the exposure to the organism and the host immune status. In addition, patients with infections caused by thes...

journal_title：Journal of clinical microbiology

authors： Babady NE,Buckwalter SP,Hall L,Le Febre KM,Binnicker MJ,Wengenack NL

更新日期：2011-09-01 00:00:00

abstract：:An optimized reverse transcription (RT)-PCR protocol for the intravitam detection of rabies virus genomic RNA was tested with clinical samples obtained from 28 patients suspected of having rabies, 9 of whom were confirmed to have had rabies by postmortem examination. RT-PCR using saliva combined with an immunofluoresc...

journal_title：Journal of clinical microbiology

authors： Crepin P,Audry L,Rotivel Y,Gacoin A,Caroff C,Bourhy H

更新日期：1998-04-01 00:00:00

abstract：:Nonfermenting coryneform bacteria identified as Brevibacterium spp. were isolated from routine clinical specimens. Four strains were derived from peritoneal fluid and has presumably been involved in the pathogenesis of continuous ambulatory peritoneal dialysis peritonitis. Another five isolates most probably represent...

journal_title：Journal of clinical microbiology

authors： Gruner E,Pfyffer GE,von Graevenitz A

更新日期：1993-06-01 00:00:00

abstract：:Detection of herpes simplex virus in clinical specimens by the suspension-infection technique was compared with detection by conventional cell culture. The sensitivity and specificity of the suspension-infection technique compared with those of conventional culture were 95.9 to 98.2% and 97.5 to 100%, respectively, de...

journal_title：Journal of clinical microbiology

authors： Luker G,Chow C,Richards DF,Johnson FB

更新日期：1991-07-01 00:00:00