Marked differences in quantity of infectious human immunodeficiency virus type 1 detected in persons with controlled plasma viremia by a simple enhanced culture method.

abstract：:A monoclonal antibody specific for group D Salmonella antigen 9 was used in an indirect enzyme-linked immunosorbent assay (ELISA) for detecting the antigen in urine specimens collected from patients with clinical typhoid fever in Jakarta, Indonesia. The ELISA had a sensitivity of 95% in identifying patients in whom Sa...

journal_title：Journal of clinical microbiology

authors： Chaicumpa W,Ruangkunaporn Y,Burr D,Chongsa-Nguan M,Echeverria P

更新日期：1992-09-01 00:00:00

abstract：:Cytomegalovirus (CMV) antigenemia was directly detected in polymorphonuclear leukocytes (PMNLs) from transplant recipients by using flow cytometry (FC). Two fixation and permeabilization methods and seven anti-CMV monoclonal antibodies (MAbs) were evaluated. 1C3, SL20, and NEA-9221 MAbs were more efficacious. The anti...

journal_title：Journal of clinical microbiology

authors： Imbert-Marcille BM,Robillard N,Poirier AS,Coste-Burel M,Cantarovich D,Milpied N,Billaudel S

更新日期：1997-10-01 00:00:00

abstract：:We isolated and characterized a Vibrio cholerae O139 Bengal-specific DNA region by arbitrary PCR. The fragment contains open reading frames encoding two potential glycosyltransferases possibly involved in capsular polysaccharide or lipopolysaccharide biosynthesis. In order to evaluate the possibility that this region ...

journal_title：Journal of clinical microbiology

authors： Falklind S,Stark M,Albert MJ,Uhlén M,Lundeberg J,Weintraub A

更新日期：1996-12-01 00:00:00

abstract：:Ten cases of typhoid fever occurred between 24 August and 1 September 1986 in the vicinity of Silver Spring, Md. Shrimp salad served in a fast-food restaurant was implicated as the source of infection. Stool cultures were obtained from 104 employees, and serum Vi antibodies were assayed in 97 of the employees. Salmone...

journal_title：Journal of clinical microbiology

authors： Lin FY,Becke JM,Groves C,Lim BP,Israel E,Becker EF,Helfrich RM,Swetter DS,Cramton T,Robbins JB

更新日期：1988-06-01 00:00:00

abstract：:To select a method for detecting methicillin-resistant Staphylococcus aureus (MRSA) in nasal swabs, we compared BD GeneOhm MRSA PCR and various culture media (mannitol salt agar with cefoxitin, MRSASelect, CHROMagar MRSA, and Spectra MRSA). While PCR detection of MRSA was more rapid, MRSASelect and Spectra MRSA demons...

journal_title：Journal of clinical microbiology

authors： Bischof LJ,Lapsley L,Fontecchio K,Jacosalem D,Young C,Hankerd R,Newton DW

更新日期：2009-07-01 00:00:00

abstract：:Small round-structured viruses (SRSVs) or Norwalk-like viruses have been implicated as an important causative agent of gastroenteritis outbreaks. We used the relatively newly developed recombinant enzyme immunoassays (EIAs) to determine the seroprevalence of Norwalk virus (NV) and Mexico virus (MxV) in a family-based ...

journal_title：Journal of clinical microbiology

authors： Smit TK,Steele AD,Peenze I,Jiang X,Estes MK

更新日期：1997-09-01 00:00:00

abstract：:The sensitivities of BHK-21 (C-13) and murine neuroblastoma (C-1300; clone NA) cells for the isolation of small quantities of a street strain rabies virus were compared. Suspensions of brain from mice sacrificed prior to the onset of clinical signs of rabies were used to stimulate weakly positive diagnostic specimens....

journal_title：Journal of clinical microbiology

authors： Rudd RJ,Trimarchi CV

更新日期：1987-08-01 00:00:00

abstract：:Potential causes of congenital infection include Toxoplasma gondii and viruses such as cytomegalovirus (CMV), enterovirus, hepatitis C virus, herpes simplex virus types 1 and 2 (HSV-1 and -2), human herpesvirus types 6, 7, and 8, lymphocytic choriomeningitis virus, parvovirus, rubella virus, and varicella-zoster virus...

journal_title：Journal of clinical microbiology

authors： McIver CJ,Jacques CF,Chow SS,Munro SC,Scott GM,Roberts JA,Craig ME,Rawlinson WD

更新日期：2005-10-01 00:00:00

abstract：:Two multiplex PCR enzyme immunoassays (PCR-EIAs) were developed for Staphylococcus aureus exotoxin gene screening as an alternative to the conventional biological assays, which depend on detectable amounts of toxins produced. One set of oligonucleotide primers and probes was designed to search for enterotoxin A to E g...

journal_title：Journal of clinical microbiology

authors： Becker K,Roth R,Peters G

更新日期：1998-09-01 00:00:00

abstract：:Tularemia is a potentially fatal disease that is caused by the highly infectious and zoonotic pathogen Francisella tularensis. Despite the monomorphic nature of sequenced F. tularensis genomes, there is a significant degree of plasticity in the organization of genetic elements. The observed variability in these genome...

journal_title：Journal of clinical microbiology

authors： Larson MA,Fey PD,Bartling AM,Iwen PC,Dempsey MP,Francesconi SC,Hinrichs SH

更新日期：2011-08-01 00:00:00

abstract：:Detection of group B streptococcus (GBS) antigen in urine by latex particle agglutination (LPA) may facilitate the rapid diagnosis of GBS sepsis. We sought to compare three commercial LPA assays with specimens that were spiked with type-specific antigen, group-specific antigen, or type III organisms. There were sensit...

journal_title：Journal of clinical microbiology

authors： Ascher DP,Wilson S,Fischer GW

更新日期：1991-12-01 00:00:00

abstract：:The NucliSENS easyMAG automated system was compared to the column-based Qiagen method for Epstein-Barr virus (EBV) or cytomegalovirus (CMV) DNA extraction from whole blood before viral load determination using the corresponding R-gene amplification kits. Both extraction techniques exhibited a total agreement of 81.3% ...

journal_title：Journal of clinical microbiology

authors： Pillet S,Bourlet T,Pozzetto B

更新日期：2009-11-01 00:00:00

abstract：:We report an unusual case of culture-proven pneumococcal and meningococcal mixed meningitis in an 18-month-old girl. The patient responded well to antimicrobial therapy and recovered completely without sequelae. No underlying condition could be demonstrated except a rhinitis of unknown etiology 2 days before the onset...

journal_title：Journal of clinical microbiology

authors： Marchandin H,Ventura V,Alonso JM,Van de Perre P

更新日期：2005-03-01 00:00:00

abstract：:Candida dubliniensis is often associated with C. albicans in cultures. Easy-to-perform selective isolation procedures for these closely related species do not exist. Therefore, we evaluated previously described discriminatory phenotypic markers for C. dubliniensis. A total of 150 oral rinses from human immunodeficienc...

journal_title：Journal of clinical microbiology

authors： Tintelnot K,Haase G,Seibold M,Bergmann F,Staemmler M,Franz T,Naumann D

更新日期：2000-04-01 00:00:00

abstract：:A microhematocrit concentration method (MH) for immediate diagnosis of Chagas' disease during the acute stage or in congenital cases was standardized. Parasitemia as low as 1,000 parasites per ml was detected, after centrifugation of six 50-microliters capillary tubes, by 10-min microscopic observation of each buffy c...

journal_title：Journal of clinical microbiology

authors： Feilij H,Muller L,Gonzalez Cappa SM

更新日期：1983-08-01 00:00:00

abstract：:The 37-kDa protein (P37) of Borrelia burgdorferi is an antigen that elicits an early immunoglobulin M (IgM) antibody response in Lyme disease patients. The P37 gene was cloned from a B. burgdorferi genomic library by screening with antibody from a Lyme disease patient who had developed a prominent humoral response to ...

journal_title：Journal of clinical microbiology

authors： Gilmore RD Jr,Murphree RL,James AM,Sullivan SA,Johnson BJ

更新日期：1999-03-01 00:00:00

abstract：:In the face of concerns over an influenza pandemic, identification of virulent influenza A virus isolates must be obtained quickly for effective responses. Rapid subtype identification, however, is difficult even in well-equipped virology laboratories or is unobtainable in the field under more austere conditions. Here...

journal_title：Journal of clinical microbiology

authors： Lodes MJ,Suciu D,Elliott M,Stover AG,Ross M,Caraballo M,Dix K,Crye J,Webby RJ,Lyon WJ,Danley DL,McShea A

更新日期：2006-04-01 00:00:00

abstract：:Southern blot hybridization with the Ca3 probe and the C fragment of the Ca3 probe was used to assess the genetic relatedness of Candida albicans strains from one patient with recurrent C. albicans infection in whom the same strain was maintained, one patient in whom the infecting strain was replaced, and their male s...

journal_title：Journal of clinical microbiology

authors： Schröppel K,Rotman M,Galask R,Mac K,Soll DR

更新日期：1994-11-01 00:00:00

abstract：:There are several convenient and accurate molecular assays to detect respiratory bacterial infection. The NeoPlex RB-8 detection kit (NeoPlex RB-8) is a new multiplex real-time PCR assay that simultaneously detects Streptococcus pneumoniae, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, Haemo...

journal_title：Journal of clinical microbiology

authors： Kim JW,Hong SS,Lee IS,Chi HY,Kim SO,Kim HN,Hong SP

更新日期：2020-01-28 00:00:00

abstract：:The modification of an auxanographic method used in yeast species identification to determine potassium nitrate (KNO3) assimilation resulted in a simple and inexpensive KNO3 assimilation test medium. The medium provided accurate and reliable results in less than 24 h. ...

journal_title：Journal of clinical microbiology

authors： Pincus DH,Salkin IF,Hurd NJ,Levy IL,Kemna MA

更新日期：1988-02-01 00:00:00

abstract：:Sepsis is a major cause of death worldwide among nonhospitalized people and hospitalized patients. A wide range of pathogens are involved, and the correct identification and correct antimicrobial therapy are critical to ensure optimal clinical outcomes. With the recent introduction of matrix-assisted laser desorption ...

journal_title：Journal of clinical microbiology

authors： Foster AG

更新日期：2013-11-01 00:00:00

abstract：:Immunoblots were used to study the immunoglobulin G response to Borrelia burgdorferi in experimentally and naturally exposed dogs. Adsorption studies confirmed that the antibodies were specific for B. burgdorferi. Experimentally exposed dogs were asymptomatic. Naturally exposed dogs included both asymptomatic animals ...

journal_title：Journal of clinical microbiology

authors： Greene RT,Walker RL,Nicholson WL,Heidner HW,Levine JF,Burgess EC,Wyand M,Breitschwerdt EB,Berkhoff HA

更新日期：1988-04-01 00:00:00

abstract：:Laboratory and clinical diagnostic classification of seropositive individuals, followed by treatment and supportive therapy, is an established component of Chagas' disease control in areas where this disease is endemic. However, most Chagas' disease patients live in remote areas where neither equipped laboratories nor...

journal_title：Journal of clinical microbiology

authors： Roddy P,Goiri J,Flevaud L,Palma PP,Morote S,Lima N,Villa L,Torrico F,Albajar-Viñas P

更新日期：2008-06-01 00:00:00

abstract：:A DNA microarray for detection of Campylobacter spp. was recently developed and applied to detect Campylobacter spp. directly from chicken feces. Sixty-five pooled chicken cloacal swab samples from 650 individual broiler chickens were included in the study. The results of Campylobacter sp. detection obtained with DNA ...

journal_title：Journal of clinical microbiology

authors： Keramas G,Bang DD,Lund M,Madsen M,Bunkenborg H,Telleman P,Christensen CB

更新日期：2004-09-01 00:00:00

abstract：:In West African countries such as Ghana, efficient human immunodeficiency virus (HIV) testing is a priority in the fight against AIDS. A new immunochromatographic rapid test, Determine HIV-1/2 (Abbott Diagnostics, North Chicago, Ill.), that detects antibodies against HIV type 1 (HIV-1) and/or HIV-2 was evaluated using...

journal_title：Journal of clinical microbiology

authors： Aidoo S,Ampofo WK,Brandful JA,Nuvor SV,Ansah JK,Nii-Trebi N,Barnor JS,Apeagyei F,Sata T,Ofori-Adjei D,Ishikawa K

更新日期：2001-07-01 00:00:00

abstract：:We report herein a case of Bartholin's gland abscess caused by Brucella melitensis. Clinical microbiology laboratory workers in areas where this disease is endemic should be familiar with the bacteriological features of this organism and consider the possibility of a brucellar etiology in a broad range of clinical set...

journal_title：Journal of clinical microbiology

authors： Peled N,David Y,Yagupsky P

更新日期：2004-02-01 00:00:00

abstract：:A new method for determining the MICs for Aspergillus species with slant-shaking incubation is described. The method was rapid (24 h), produced sharp visual end points, and showed high reproducibility. Additionally, MICs measured by this method correlated well with, as well as were two- to sixfold lower than, those by...

journal_title：Journal of clinical microbiology

authors： Yamada H,Kohno S,Maesaki S,Koga H,Kaku M,Hara K,Tanaka H

更新日期：1993-04-01 00:00:00

abstract：:We describe two cases of eumycetoma in the legs. The infections could not be adequately diagnosed by classical mycology, but the causative agents were successfully identified as Madurella mycetomatis by species-specific PCR and DNA sequencing. ...

journal_title：Journal of clinical microbiology

authors： Ahmed AO,Desplaces N,Leonard P,Goldstein F,De Hoog S,Verbrugh H,van Belkum A

更新日期：2003-12-01 00:00:00

abstract：:The detection of immunoglobulin M (IgM) antibodies to cytomegalovirus-induced late antigens by an indirect immunofluorescence assay was improved by the use of monoclonal antibodies to human IgM. Nonspecific background fluorescence was absent, facilitating the reading of the slides and the detection of a specific fluor...

journal_title：Journal of clinical microbiology

authors： Zerbini M,Musiani M,Gentilomi G,La Placa M

更新日期：1986-07-01 00:00:00

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial pneumonia. To characterize pathogen-derived and host-related factors in intensive care unit (ICU) patients with MRSA pneumonia, we evaluated the Improving Medicine through Pathway Assessment of Critical Therapy in Hospital-Acquired Pneum...

journal_title：Journal of clinical microbiology

authors： Haque NZ,Arshad S,Peyrani P,Ford KD,Perri MB,Jacobsen G,Reyes K,Scerpella EG,Ramirez JA,Zervos MJ

更新日期：2012-05-01 00:00:00