Multilocus sequence typing of methicillin-resistant Staphylococcus aureus from an area of low endemicity by real-time PCR.

abstract：:Pneumonia has been identified as a major cause of poor lamb survival in indigenous herds of Rocky Mountain bighorn sheep (Ovis canadensis canadensis) in central Idaho. Pasteurella haemolytica was isolated from five adult Rocky Mountain bighorn ewes captured from a free-ranging herd in central Idaho. The lambs from two...

journal_title：Journal of clinical microbiology

authors： Jaworski MD,Ward AC,Hunter DL,Wesley IV

更新日期：1993-04-01 00:00:00

abstract：:A real-time PCR assay was developed targeting the bla(KPC) responsible for Klebsiella pneumoniae carbapenemase (KPC)-mediated carbapenem resistance and was validated for testing colonies or enrichment broth cultures. The assay accurately detects KPC-containing strains with high analytical specificity and sensitivity. ...

journal_title：Journal of clinical microbiology

authors： Mangold KA,Santiano K,Broekman R,Krafft CA,Voss B,Wang V,Hacek DM,Usacheva EA,Thomson RB Jr,Kaul KL,Peterson LR

更新日期：2011-09-01 00:00:00

abstract：:In order to assess the microbiologic and clinical value of primary broth culture of wound specimens collected with swabs and submitted to the laboratory in transport medium, we compared the results of primary agar culture with the results of a corresponding primary broth culture for 344 aerobic specimens and 176 anaer...

journal_title：Journal of clinical microbiology

authors： Silletti RP,Ailey E,Sun S,Tang D

更新日期：1997-08-01 00:00:00

abstract：:Aberrant filamentous forms of Escherichia coli were observed on direct Gram stain of blood cultures from a patient being treated with the beta-lactam antibiotic cephalexin. After the institution of an alternative antibiotic regimen which included a different cell wall-active agent, E. coli of normal morphology was det...

journal_title：Journal of clinical microbiology

authors： Magnussen CR,Hruska JF

更新日期：1980-11-01 00:00:00

abstract：:By two PCR-based diagnostic methods, Plasmodium malariae infections have been rediscovered at two foci in the Sichuan province of China, a region where no cases of P. malariae have been officially reported for the last 2 decades. In addition, a variant form of P. malariae which has a deletion of 19 bp and seven substi...

journal_title：Journal of clinical microbiology

authors： Liu Q,Zhu S,Mizuno S,Kimura M,Liu P,Isomura S,Wang X,Kawamoto F

更新日期：1998-11-01 00:00:00

abstract：:A new molecular assay, based on a rapid DNA extraction protocol, PCR, and hybridization to a specific probe in a nonradioactive microwell plate format was used to detect Mycoplasma pneumoniae in bronchoalveolar fluid specimens. The sensitivity of the assay was determined to be 10 to 100 organisms with M. pneumoniae re...

journal_title：Journal of clinical microbiology

authors： Kessler HH,Dodge DE,Pierer K,Young KK,Liao Y,Santner BI,Eber E,Roeger MG,Stuenzner D,Sixl-Voigt B,Marth E

更新日期：1997-06-01 00:00:00

abstract：:Two colorimetric methods that use Alamar Blue or 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) for assaying the in vitro activities of antifungal agents have been described. We report that both tests performed similarly when the antifungal activity of amphotericin B against Candida albicans wa...

journal_title：Journal of clinical microbiology

authors： Jahn B,Stüben A,Bhakdi S

更新日期：1996-08-01 00:00:00

abstract：:Four of eleven affected dogs died despite aggressive treatment during a 2015 focal outbreak of hemorrhagic gastroenteritis following a stay in a pet housing facility. Routine diagnostic investigations failed to identify a specific cause. Virus isolation from fresh necropsy tissues yielded a calicivirus with sequence h...

journal_title：Journal of clinical microbiology

authors： Renshaw RW,Griffing J,Weisman J,Crofton LM,Laverack MA,Poston RP,Duhamel GE,Dubovi EJ

更新日期：2018-04-25 00:00:00

abstract：:We report a fatal case of a chromoblastomycosis-like infection caused by a novel species of Fonsecaea in a 52-year-old immunocompetent Caucasian male from an area of chromoblastomycosis endemicity in Brazil. The patient had a 30-year history of slowly evolving, verrucous lesions on the right upper arm which gradually ...

journal_title：Journal of clinical microbiology

authors： de Azevedo CM,Gomes RR,Vicente VA,Santos DW,Marques SG,do Nascimento MM,Andrade CE,Silva RR,Queiroz-Telles F,de Hoog GS

更新日期：2015-08-01 00:00:00

abstract：:Two rapid diagnostic tests for Chlamydia trachomatis (Microtrak, Syva Co., Palo Alto, Calif.; and Chlamydiazyme, Abbott Laboratories, North Chicago, Ill.) were evaluated in comparison with growth of the organism in tissue culture for 2,030 urogenital specimens from men and women. Neither test performed as well as cult...

journal_title：Journal of clinical microbiology

authors： Hipp SS,Han Y,Murphy D

更新日期：1987-10-01 00:00:00

abstract：:Gastrointestinal disease and colonization by Helicobacter pylori were determined in 36 asymptomatic volunteers and 30 symptomatic individuals undergoing endoscopy and biopsy. Serum antibody immunoglobulin G (IgG) and IgA to H. pylori were measured by enzyme-linked immunosorbent assay. Serum antibody to a cytotoxin pro...

journal_title：Journal of clinical microbiology

authors： Leunk RD,Ferguson MA,Morgan DR,Low DE,Simor AE

更新日期：1990-06-01 00:00:00

abstract：:Beagle dogs inoculated intravenously with 1.75 x 10(9) viable Encephalitozoon cuniculi spores at 12.5 months of age were monitored for 18 months to assess infection in the adult dog. Parameters monitored included packed cell volume, total and differential leukocyte counts, and humoral and cellular responses to infecti...

journal_title：Journal of clinical microbiology

authors： Szabo JR,Shadduck JA

更新日期：1988-03-01 00:00:00

abstract：:The Autobac IDX system (General Diagnostics, Warner-Lambert Co., Morris Plains, N.J.) for rapid, semiautomated identification of gram-negative bacilli was compared with the identification methods in routine use in four laboratories. The study included 1,515 organisms representing 30 species of enteric and nonenteric b...

journal_title：Journal of clinical microbiology

authors： Kelly MT,Matsen JM,Morello JA,Smith PB,Tilton RC

更新日期：1984-04-01 00:00:00

abstract：:The molecular epidemiology of tuberculosis (TB) in Finland was studied by spoligotyping 380 Mycobacterium tuberculosis isolates. The isolates were obtained during a 1-year study period from July 2000 to June 2001 and represented 90% of new M. tuberculosis findings by culture in the whole country during the study perio...

journal_title：Journal of clinical microbiology

authors： Puustinen K,Marjamäki M,Rastogi N,Sola C,Filliol I,Ruutu P,Holmström P,Viljanen MK,Soini H

更新日期：2003-04-01 00:00:00

abstract：:Nosocomial bronchopneumonia is a frequent complication in patients with chronic intratracheal intubation. Despite targeted antibiotic treatment, production of abundant bronchial secretion containing pathogen bacteria often tends to be chronic, and so mortality drastically increases. This problem led to an investigatio...

journal_title：Journal of clinical microbiology

authors： Klekner A,Bagyi K,Bognar L,Gaspar A,Andrasi M,Szabo J

更新日期：2006-09-01 00:00:00

abstract：:Sodium polyanethole sulfonate (SPS; trade name, Liquoid) is a constituent in culture media used to grow bacteria from blood samples from patients suspected of bacteremia. SPS prevents the killing of bacteria by innate cellular and humoral factors. We analyzed the effect of SPS on the three complement activation pathwa...

journal_title：Journal of clinical microbiology

authors： Palarasah Y,Skjoedt MO,Vitved L,Andersen TE,Skjoedt K,Koch C

更新日期：2010-03-01 00:00:00

abstract：:Four commercial kits, Oxi/Ferm (OF), API 20E (AP), Minitek (MT; BBL Microbiology Systems), and Flow N/F (NF), were evaluated, without additional tests, for identification of 258 gram-negative nonfermentative rods. OF and MT were read after 48 h of incubation, and AP and NF were read after both 24 and 48 h of incubatio...

journal_title：Journal of clinical microbiology

authors： Appelbaum PC,Stavitz J,Bentz MS,von Kuster LC

更新日期：1980-08-01 00:00:00

abstract：:Pseudobacteremias in blood cultures performed on the BACTEC radiometric blood culture system have been reported. We report three cases of cross contamination with Mycobacterium avium that occurred when mycobacteria were cultured with the BACTEC 460 TB system. Malfunction of the needle sterilization heating block was d...

journal_title：Journal of clinical microbiology

authors： Vannier AM,Tarrand JJ,Murray PR

更新日期：1988-09-01 00:00:00

abstract：:Two previously demonstrated sensitive and specific enzyme immunoassays (EIAs) for rotavirus, one using polyclonal and monoclonal antisera (TestPack Rotavirus [TPK]; Abbott Laboratories) and the other using only monoclonal anti-rotavirus antibodies (Rotaclone [RTC]; Cambridge BioScience Corporation), were evaluated as ...

journal_title：Journal of clinical microbiology

authors： Dennehy PH,Gauntlett DR,Spangenberger SE

更新日期：1990-06-01 00:00:00

abstract：:About 2.5 years after the first isolation of the VanA phenotype of vancomycin-resistant Enterococcus faecium (VREM) in Poland, the first VREM strains with the VanB phenotype have emerged independently in two different Warsaw hospitals. In one of these the VREM strain was selected during the long-term antimicrobial tre...

journal_title：Journal of clinical microbiology

authors： Kawalec M,Gniadkowski M,Zielińska U,Kłos W,Hryniewicz W

更新日期：2001-02-01 00:00:00

abstract：:The sensitivity of PCR for the amplification of target nucleic acid sequences in clinical diagnostics may often be reduced due to the presence of inhibitory factors. Hemolytic serum contains a number of PCR inhibitors, one of which is hemin. In this study we have found that conventional methods of DNA extraction were ...

journal_title：Journal of clinical microbiology

authors： Klein A,Barsuk R,Dagan S,Nusbaum O,Shouval D,Galun E

更新日期：1997-07-01 00:00:00

abstract：:Members of the genus Abiotrophia, formerly known as nutritionally variant streptococci, are important pathogens causing septicemia and endocarditis. Cultivation and biochemical differentiation of Abiotrophia spp. are often difficult. Based on 16S rRNA sequences, two PCR assays for detection and identification of Abiot...

journal_title：Journal of clinical microbiology

authors： Roggenkamp A,Leitritz L,Baus K,Falsen E,Heesemann J

更新日期：1998-10-01 00:00:00

abstract：:To study the population structure of Enterococcus faecalis from Polish hospitals, 291 isolates were typed by pulsed-field gel electrophoresis and a novel multilocus sequence typing scheme (P. Ruiz-Garbajosa et al., J. Clin. Microbiol. 44:2220-2228, 2006). The isolates originated from geographically widespread medical ...

journal_title：Journal of clinical microbiology

authors： Kawalec M,Pietras Z,Daniłowicz E,Jakubczak A,Gniadkowski M,Hryniewicz W,Willems RJ

更新日期：2007-01-01 00:00:00

abstract：:The amino acids L-arginine, L-isoleucine, L-leucine, L-methionine, L-serine, L-threonine, and L-valine were essential for the growth of Legionella pneumophila in a chemically defined medium. A partial requirement for L-cysteine (or L-cystine) was also observed. A minimal medium containing only the eight required amino...

journal_title：Journal of clinical microbiology

authors： Tesh MJ,Miller RD

更新日期：1981-05-01 00:00:00

abstract：:Charcoal-horse blood agar with 40 micrograms of cephalexin per ml, charcoal-horse blood agar with 3 micrograms of lincomycin per ml, charcoal agar with 3 micrograms of lincomycin per ml, and Legionella (buffered charcoal-yeast extract) agar with 3 micrograms of lincomycin per ml were compared for isolation of Bordetel...

journal_title：Journal of clinical microbiology

authors： Hoppe JE,Schwaderer J

更新日期：1989-05-01 00:00:00

abstract：:We compared the Gen-Probe transcription-mediated amplification assay (AMP CT), the Abbott LCx assay, and the Roche COBAS AMPLICOR assay for the detection of Chlamydia trachomatis in a mixed population in urine samples. First-void urine, urethral specimens, and cervical specimens in females were obtained from 1,000 pat...

journal_title：Journal of clinical microbiology

authors： Goessens WH,Mouton JW,van der Meijden WI,Deelen S,van Rijsoort-Vos TH,Lemmens-den Toom N,Verbrugh HA,Verkooyen RP

更新日期：1997-10-01 00:00:00

abstract：:A supplemented 2% peptone broth is described for the detection of indole production by Pasteurella multocida. The 96 isolates of P. multocida that were utilized in this evaluation were indole positive within 18 to 24 h. ...

journal_title：Journal of clinical microbiology

authors： Clemons KV,Gadberry JL

更新日期：1982-04-01 00:00:00

abstract：:This case report describes the fungal dermatitis caused by Neosartorya hiratsukae infection in a household hedgehog confirmed by microscopic examination of conidiophores and DNA analyses including the internal transcribed spacer region, partial beta-tubulin, and the calmodulin gene. It is the first report of a natural...

journal_title：Journal of clinical microbiology

authors： Han JI,Na KJ

更新日期：2008-09-01 00:00:00

abstract：:We prospectively studied 156 patients with a diagnosis of community-acquired pneumonia requiring admission. Several respiratory specimens were obtained for the detection of Chlamydia pneumoniae by cell culture and PCR. Three serum samples were obtained from each patient. Serological diagnosis of a C. pneumoniae infect...

journal_title：Journal of clinical microbiology

authors： Verkooyen RP,Willemse D,Hiep-van Casteren SC,Joulandan SA,Snijder RJ,van den Bosch JM,van Helden HP,Peeters MF,Verbrugh HA

更新日期：1998-08-01 00:00:00

abstract：:Blood isolates of Salmonella enterica serovar Typhi from two recently returned Bangladeshi patients in Kuwait were ciprofloxacin resistant, with ciprofloxacin MICs of 12 mg/liter for both isolates. Both isolates had three novel gyrA mutations (55-Leu-->Trp, 87-Asp-->Ala, and 106-Gln-->Arg) and three novel parC mutatio...

journal_title：Journal of clinical microbiology

authors： Dimitrov T,Dashti AA,Albaksami O,Udo EE,Jadaon MM,Albert MJ

更新日期：2009-01-01 00:00:00