Abstract:
:The sensitivity of PCR for the amplification of target nucleic acid sequences in clinical diagnostics may often be reduced due to the presence of inhibitory factors. Hemolytic serum contains a number of PCR inhibitors, one of which is hemin. In this study we have found that conventional methods of DNA extraction were not sufficient for the removal of PCR-inhibitory compounds in hemolytic serum. We have therefore compared the efficiency of several commercial and noncommercial methods of nucleic acid purification from hemolytic serum samples prior to PCR amplification. Separation with the QIAamp HCV kit, dialysis with Millipore filters, and bovine serum albumin absorption were all found to be suitable extraction methods for eliminating inhibitors from hemolytic serum for PCR amplification. Using these methods we were able to detect very low levels of hepatitis B virus DNA in hemolytic serum.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Klein A,Barsuk R,Dagan S,Nusbaum O,Shouval D,Galun Edoi
10.1128/JCM.35.7.1897-1899.1997subject
Has Abstractpub_date
1997-07-01 00:00:00pages
1897-9issue
7eissn
0095-1137issn
1098-660Xjournal_volume
35pub_type
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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abstract::The development of a satisfactory means to reliably distinguish between the two closely related species Candida albicans and Candida dubliniensis in the clinical mycology laboratory has proved difficult because these two species are phenotypically so similar. In this study, we have detected homologues of the pH-regula...
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更新日期:2006-07-01 00:00:00
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