Comparison of GenomEra C. difficile and Xpert C. difficile as confirmatory tests in a multistep algorithm for diagnosis of Clostridium difficile infection.

Abstract:

:We compared two multistep diagnostic algorithms based on C. Diff Quik Chek Complete and, as confirmatory tests, GenomEra C. difficile and Xpert C. difficile. The sensitivity, specificity, positive predictive value, and negative predictive value were 87.2%, 99.7%, 97.1%, and 98.3%, respectively, for the GenomEra-based algorithm and 89.7%, 99.4%, 95.5%, and 98.6%, respectively, for the Xpert-based algorithm. GenomEra represents an alternative to Xpert as a confirmatory test of a multistep algorithm for Clostridium difficile infection (CDI) diagnosis.

journal_name

J Clin Microbiol

authors

Alcalá L,Reigadas E,Marín M,Fernández-Chico A,Catalán P,Bouza E

doi

10.1128/JCM.03093-14

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

332-5

issue

1

eissn

0095-1137

issn

1098-660X

pii

JCM.03093-14

journal_volume

53

pub_type

杂志文章
  • Reemergence of emm1 and a changed superantigen profile for group A streptococci causing invasive infections: results from a nationwide study.

    abstract::Between 1999 and 2002, 496 invasive group A streptococcal (GAS) isolates from clinical microbiological departments in Denmark and subsequently 487 (98%) questionnaires from the clinicians treating the patients were received as part of a national surveillance. emm types and streptococcal superantigen (SAg) genes were d...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.4.1789-1796.2005

    authors: Ekelund K,Skinhøj P,Madsen J,Konradsen HB

    更新日期:2005-04-01 00:00:00

  • Asymptomatic respiratory tract infection with Chlamydia pneumoniae TWAR.

    abstract::Chlamydia pneumoniae is a newly recognized organism associated with respiratory tract infections. Asymptomatic infection with C. pneumoniae, although it has been suggested to occur, has not been previously documented. We describe two asymptomatic individuals infected with this organism; these infections demonstrate th...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.29.9.2082-2083.1991

    authors: Hyman CL,Augenbraun MH,Roblin PM,Schachter J,Hammerschlag MR

    更新日期:1991-09-01 00:00:00

  • Comparative evaluation of a commercially available automated system for extraction of viral DNA from whole blood: application to monitoring of epstein-barr virus and cytomegalovirus load.

    abstract::The NucliSENS easyMAG automated system was compared to the column-based Qiagen method for Epstein-Barr virus (EBV) or cytomegalovirus (CMV) DNA extraction from whole blood before viral load determination using the corresponding R-gene amplification kits. Both extraction techniques exhibited a total agreement of 81.3% ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01497-09

    authors: Pillet S,Bourlet T,Pozzetto B

    更新日期:2009-11-01 00:00:00

  • Adhesion to a human cell line by Escherichia coli strains isolated during urinary tract infections.

    abstract::It has been shown that some, but not all, Escherichia coli strains isolated from urine adhere, in vitro, to the surface of uroepithelial or vaginal cells. In the present study, 212 strains, isolated from urine of 212 infected patients, were tested for adhesion by using an in vitro human cell line assay. A variable deg...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.10.4.563-566.1979

    authors: Chabanon G,Hartley CL,Richmond MH

    更新日期:1979-10-01 00:00:00

  • Rotavirus gastroenteritis and weather.

    abstract::During 5.5 years of a study in Washington, D.C., hospitalizations of children for rotavirus gastroenteritis tended to be more common after a month of cold or dry weather than after a corresponding calendar month of warm or wet weather. Overall, there were 84% more (178 versus 97) inpatients with rotavirus gastroenteri...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.16.3.478-482.1982

    authors: Brandt CD,Kim HW,Rodriguez WJ,Arrobio JO,Jeffries BC,Parrott RH

    更新日期:1982-09-01 00:00:00

  • Variation in electrophoretic karyotype and antifungal susceptibility of clinical isolates of Cryptococcus neoformans at a university-affiliated teaching hospital from 1987 to 1994.

    abstract::Ninety-eight isolates of Cryptococcus neoformans were collected from 30 patients at the University of Iowa Hospitals and Clinics from December 1987 through December 1994. The susceptibility of each isolate was determined against fluconazole, itraconazole, amphotericin B, and flucytosine. Of the 98 isolates, 53 were re...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.36.12.3653-3656.1998

    authors: Klepser ME,Pfaller MA

    更新日期:1998-12-01 00:00:00

  • Increased expression of Candida albicans secretory proteinase, a putative virulence factor, in isolates from human immunodeficiency virus-positive patients.

    abstract::The increased prevalence and the severity of oropharyngeal candidiasis in human immunodeficiency virus (HIV)-positive patients are attributed exclusively to the virus-induced immune deficiency of the host. The present study was aimed at answering the question of whether Candida albicans secretory proteinase, a putativ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.10.2543-2549.1995

    authors: Ollert MW,Wende C,Görlich M,McMullan-Vogel CG,Borg-von Zepelin M,Vogel CW,Korting HC

    更新日期:1995-10-01 00:00:00

  • New multiple antigenic peptide-based enzyme immunoassay for detection of simian immunodeficiency virus infection in nonhuman primates and humans.

    abstract::Infections with human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2, respectively) are zoonotic infections. In Africa, the potential exists for additional cross-species transmissions from at least 33 different species of simian immunodeficiency virus (SIV)-infected nonhuman primates (NHPs) through hunting and ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.42.11.5161-5169.2004

    authors: Ndongmo CB,Switzer WM,Pau CP,Zeh C,Schaefer A,Pieniazek D,Folks TM,Kalish ML

    更新日期:2004-11-01 00:00:00

  • Serotyping of Canadian isolates of Treponema hyodysenteriae and description of two new serotypes.

    abstract::A total of 30 isolates of Treponema hyodysenteriae collected in the Saint-Hyacinthe (Quebec, Canada) area were serotyped by agar gel double immunodiffusion by using extracted lipopolysaccharide and hyperimmune rabbit antisera. Only 17% (5 of 30) of the isolates were typed with antisera specific for each of the seven k...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.29.12.2794-2797.1991

    authors: Li ZS,Bélanger M,Jacques M

    更新日期:1991-12-01 00:00:00

  • Comparative Performance of SARS-CoV-2 Detection Assays Using Seven Different Primer-Probe Sets and One Assay Kit.

    abstract::Nearly 400,000 people worldwide are known to have been infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) beginning in December 2019. The virus has now spread to over 168 countries including the United States, where the first cluster of cases was observed in the Seattle metropolitan area in Was...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00557-20

    authors: Nalla AK,Casto AM,Huang MW,Perchetti GA,Sampoleo R,Shrestha L,Wei Y,Zhu H,Jerome KR,Greninger AL

    更新日期:2020-05-26 00:00:00

  • Two cases of subcutaneous infection due to Phaeoacremonium spp.

    abstract::We describe two cases in Brazil of human subcutaneous infections due to Phaeoacremonium spp. The first case was caused by Phaeoacremonium aleophilum. The patient presented with a unique fistulized nodule on the left ankle. The fungus was detected by direct microscopic examination and was isolated repeatedly from mater...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.3.1332-1336.2003

    authors: Guarro J,Alves SH,Gené J,Grazziotin NA,Mazzuco R,Dalmagro C,Capilla J,Zaror L,Mayayo E

    更新日期:2003-03-01 00:00:00

  • New clustered regularly interspaced short palindromic repeat locus spacer pair typing method based on the newly incorporated spacer for Salmonella enterica.

    abstract::A clustered regularly interspaced short palindromic repeat (CRISPR) typing method has recently been developed and used for typing and subtyping of Salmonella spp., but it is complicated and labor intensive because it has to analyze all spacers in two CRISPR loci. Here, we developed a more convenient and efficient meth...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00696-14

    authors: Li H,Li P,Xie J,Yi S,Yang C,Wang J,Sun J,Liu N,Wang X,Wu Z,Wang L,Hao R,Wang Y,Jia L,Li K,Qiu S,Song H

    更新日期:2014-08-01 00:00:00

  • Modified thioglycolate medium: a simple and reliable means for detection of Trichomonas vaginalis.

    abstract::Despite the declining rate of sexually transmitted diseases in developed countries, trichomoniasis is still one of the most common venereal infections. While diagnosis of this condition is commonly based on the microscopic wet-mount method, culture remains the most accurate single procedure for detecting the presence ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.34.10.2630-2631.1996

    authors: Poch F,Levin D,Levin S,Dan M

    更新日期:1996-10-01 00:00:00

  • Three-way comparison of BBL CHROMagar MRSA II, MRSASelect, and spectra MRSA for detection of methicillin-resistant Staphylococcus aureus isolates in nasal surveillance cultures.

    abstract::Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of hospital-acquired and life-threatening infections. Active surveillance programs for MRSA utilize either molecular or culture-based methods. A prospective study was performed to compare the performance of selective and differential chromogenic media...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02022-12

    authors: Denys GA,Renzi PB,Koch KM,Wissel CM

    更新日期:2013-01-01 00:00:00

  • Comparison of Four Serological Methods and Two Reverse Transcription-PCR Assays for Diagnosis and Surveillance of Zika Virus Infection.

    abstract::Zika virus (ZIKV) is a mosquito-borne flavivirus that is responsible for recent explosive epidemics in the Americas. Notably, ZIKV infection during pregnancy has been found to cause congenital birth defects, including microcephaly, and ZIKV has been associated with Guillain-Barré syndrome in adults. Diagnosis and surv...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01785-17

    authors: Balmaseda A,Zambrana JV,Collado D,García N,Saborío S,Elizondo D,Mercado JC,Gonzalez K,Cerpas C,Nuñez A,Corti D,Waggoner JJ,Kuan G,Burger-Calderon R,Harris E

    更新日期:2018-02-22 00:00:00

  • Comparison of identifications of human and animal source gram-negative bacteria by API 20E and crystal E/NF systems.

    abstract::This study compared the abilities of API 20E and BBL Crystal E/NF identification systems to correctly identify human and animal source gram-negative bacilli of known identifications, as provided by the American Type Culture Collection, Rockville, Md., and the Research Diagnostic and Investigative Laboratory, Columbia,...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.35.1.213-216.1997

    authors: Peele D,Bradfield J,Pryor W,Vore S

    更新日期:1997-01-01 00:00:00

  • A novel extraction method combining plasma with a whole-blood fraction shows excellent sensitivity and reproducibility for patients at high risk for invasive aspergillosis.

    abstract::Diagnosis of invasive aspergillosis (IA) is still a major problem in routine clinical practice. Early diagnosis is essential for a good patient prognosis. PCR is a highly sensitive method for the detection of nucleic acids and could play an important role in improving the diagnosis of fungal infections. Therefore, a n...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00523-12

    authors: Springer J,Schloßnagel H,Heinz W,Doedt T,Soeller R,Einsele H,Loeffler J

    更新日期:2012-08-01 00:00:00

  • Characterization and description of "Campylobacter upsaliensis" isolated from human feces.

    abstract::During a 3-year period, "Campylobacter upsaliensis" was isolated from 99 patients. Phenotypic characterization and numerical analysis of protein electrophoregrams showed evidence that "C. upsaliensis" is a distinct Campylobacter species with unique characteristics. The MBCs of 13 antibiotics were determined. In genera...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.5.1039-1046.1990

    authors: Goossens H,Pot B,Vlaes L,Van den Borre C,Van den Abbeele R,Van Naelten C,Levy J,Cogniau H,Marbehant P,Verhoef J

    更新日期:1990-05-01 00:00:00

  • Atypical Yersinia enterocolitica: clinical and epidemiological parameters.

    abstract::Infections due to biochemically typical Yersinia enterocolitica usually present as gastroenteritis, mesenteric lymphadenitis, terminal ileitis, and septicemia often with visceral abscesses. In these instances, the isolates have been biochemically typical and of well-established serotypes, namely 0:3 or 0:9 and, in the...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: Bottone EJ

    更新日期:1978-06-01 00:00:00

  • Evaluation of Staph ID 32 system and Staph-Zym system for identification of coagulase-negative staphylococci.

    abstract::The purpose of the investigation was to evaluate two commercially available identification systems: a new modification of the Staph-Zym system (Rosco, Tåstrup, Denmark) and the Staph ID 32 API system (API System, BioMérieux, Paris, France). A local standard method to be used in routine laboratories was also evaluated....

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.5.1150-1153.1995

    authors: Renneberg J,Rieneck K,Gutschik E

    更新日期:1995-05-01 00:00:00

  • Detection of cytomegalovirus antibody with latex agglutination.

    abstract::Transfusion-acquired cytomegalovirus (CMV) infections should be prevented in seronegative immunocompromised patients by providing blood products from donors who are also seronegative. Latex agglutination was investigated as a simple and rapid method for detecting antibody against CMV. Latex beads were coated with CMV ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.22.1.68-70.1985

    authors: Adler SP,McVoy M,Biro VG,Britt WJ,Hider P,Marshall D

    更新日期:1985-07-01 00:00:00

  • CHROMagar Yersinia, a new chromogenic agar for screening of potentially pathogenic Yersinia enterocolitica isolates in stools.

    abstract::CHROMagar Yersinia (CAY) is a new chromogenic medium for the presumptive detection of virulent Yersinia enterocolitica in stools. Based on a comparative analysis of 1,494 consecutive stools from hospitalized patients, CAY was found to be just as sensitive as the reference medium (cefsulodin-irgasan-novobiocin agar) bu...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02903-12

    authors: Renaud N,Lecci L,Courcol RJ,Simonet M,Gaillot O

    更新日期:2013-04-01 00:00:00

  • Correlation of oxacillin MIC with mecA gene carriage in coagulase-negative staphylococci.

    abstract::The National Committee for Clinical Laboratory Standards has recently changed the oxacillin breakpoint from >/=4 mg/liter to >/=0. 5 mg/liter to detect methicillin-resistant coagulase-negative staphylococci (CoNS) because the previous breakpoint lacked sensitivity. To determine the correlation between the new oxacilli...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.38.2.752-754.2000

    authors: Hussain Z,Stoakes L,Massey V,Diagre D,Fitzgerald V,El Sayed S,Lannigan R

    更新日期:2000-02-01 00:00:00

  • Use of Haemophilus test medium for broth microdilution antimicrobial susceptibility testing of Streptococcus pneumoniae.

    abstract::A recently described medium (Haemophilus test medium [HTM]) for antimicrobial susceptibility testing of Haemophilus influenzae was evaluated in this study for broth microdilution testing of Streptococcus pneumoniae. A total of 137 clinical isolates was tested against 11 antimicrobial agents, using Mueller-Hinton broth...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.3.430-434.1990

    authors: Jorgensen JH,Maher LA,Howell AW

    更新日期:1990-03-01 00:00:00

  • vanA and vanB incorporate into an endemic ampicillin-resistant vancomycin-sensitive Enterococcus faecium strain: effect on interpretation of clonality.

    abstract::Clonal spread and horizontal transfer in the spread of vancomycin resistance genes were investigated. Multiplex PCR, pulsed-field gel electrophoresis (PFGE), hybridization of enterococcal plasmids with the vanA and vanB probes, and sequencing of a fragment of vanB were used in the analysis. Before May 1996, 12 vancomy...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.12.3934-3939.1999

    authors: Suppola JP,Kolho E,Salmenlinna S,Tarkka E,Vuopio-Varkila J,Vaara M

    更新日期:1999-12-01 00:00:00

  • Isothermal microcalorimetry to study drugs against Schistosoma mansoni.

    abstract::Alternative antischistosomal drugs are required since praziquantel is virtually the only drug available for treatment and morbidity control of schistosomiasis. Manual microscopic reading is the current "gold standard" to assess the in vitro antischistosomal properties of test drugs; however, it is labor-intensive, sub...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02382-10

    authors: Manneck T,Braissant O,Haggenmüller Y,Keiser J

    更新日期:2011-04-01 00:00:00

  • Production of monoclonal antibody against Pneumocystis carinii by using a hybrid of rat spleen and mouse myeloma cells.

    abstract::It has been difficult to obtain pure Pneumocystis carinii antigen either from cultures or from infected lungs for use in producing a specific antibody against P. carinii. This report describes an approach toward producing a monoclonal antibody that bypasses the antigen purification steps. P. carinii infection was deve...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.23.3.505-508.1986

    authors: Lee CH,Bolinger CD,Bartlett MS,Kohler RB,Wilde CE 3rd,Smith JW

    更新日期:1986-03-01 00:00:00

  • Global multilocus sequence typing analysis of Mycoplasma bovis isolates reveals two main population clusters.

    abstract::Mycoplasma bovis is a major bovine pathogen associated with bovine respiratory disease complex and is responsible for substantial economic losses worldwide. M. bovis is also associated with other clinical presentations in cattle, including mastitis, otitis, arthritis, and reproductive disorders. To gain a better under...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01910-14

    authors: Rosales RS,Churchward CP,Schnee C,Sachse K,Lysnyansky I,Catania S,Iob L,Ayling RD,Nicholas RA

    更新日期:2015-03-01 00:00:00

  • Comparison of the B-D urine culture kit with a standard culture method and with the MS-2.

    abstract::A total of 100 random clinical urine specimens were divided and used to compare Becton, Dickinson & Co. B-D Urine Culture Kit-treated urine (BDU) with refrigerated urine (RU). Each RU-BDU pair was cultured with the standard calibrated loop-plate method and also screened with the Abbott Laboratories MS-2 system at 0, 5...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.17.2.327-331.1983

    authors: Hubbard WA,Shalis PJ,McClatchey KD

    更新日期:1983-02-01 00:00:00

  • Detection of Porphyromonas gingivalis from saliva by PCR by using a simple sample-processing method.

    abstract::Simple sample-processing methods for PCR detection of Porphyromonas gingivalis, a major pathogen causing adult periodontitis, from saliva were studied. The ability to detect P. gingivalis from 118 salivary samples by PCR after boiling and Chelex 100 processing was compared with bacterial culture. P. gingivalis was det...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.36.1.157-160.1998

    authors: Mättö J,Saarela M,Alaluusua S,Oja V,Jousimies-Somer H,Asikainen S

    更新日期:1998-01-01 00:00:00