Abstract:
:The conventional phage typing scheme proposed by S. Basu and S. Mukerjee (Experientia 24:299-300, 1968) has been used routinely for identification of the strains at the Vibrio Phage Reference Laboratory since 1968. However, because of limitations of this scheme, a new phage typing scheme using five newly isolated phages was incorporated into the conventional scheme. A different definition of routine test dilution (almost confluent lysis) was found to be more useful than the one previously used (confluent lysis). The 1,000 strains tested could be clustered into 27 types with the five new phages. With the new scheme of 10 phages (5 new phages and 5 phages of Basu and Mukerjee), the 1,000 strains could be grouped into 146 types. The new phages were different from each other and also from those of Basu and Mukerjee, as revealed by lytic pattern, electron microscopy, restriction endonuclease digestion, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and antiphage antiserum studies. With the new typing scheme, 99.6% of the strains were typeable. Phage type 115 was the most common and includes 119 (11.9%) of the 1,000 strains tested. Next most common were phage types 142 (9.4%), 143 (7.0%), 104 and 116 (both 5.4%), 3 (5.3%), 5 (4.1%), 4 (3.9%), 24 (2.1%), and 100 (1.7%). The larger number of types would be useful for further classification of the strains for epidemiological purposes. This newly developed scheme is highly applicable to, and could be widely adopted for, phage typing of Vibrio cholerae O1 biotype El Tor strains.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Chattopadhyay DJ,Sarkar BL,Ansari MQ,Chakrabarti BK,Roy MK,Ghosh AN,Pal SCdoi
10.1128/JCM.31.6.1579-1585.1993subject
Has Abstractpub_date
1993-06-01 00:00:00pages
1579-85issue
6eissn
0095-1137issn
1098-660Xjournal_volume
31pub_type
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:1998-06-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章,多中心研究
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更新日期:1995-03-01 00:00:00
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更新日期:2012-06-01 00:00:00
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journal_title:Journal of clinical microbiology
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更新日期:1993-02-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:1999-12-01 00:00:00
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更新日期:1996-03-01 00:00:00
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