Exoantigen Studies of Sporothrix schenckii, Ceratocystis minor, and Graphium penicilliodes cultures.

abstract：:As contagious bovine pleuropneumonia (CBPP) is spreading fast in many African countries, there is an increasing demand for rapid and sensitive diagnostic methods that can be used to confirm the initial diagnosis based on clinical symptoms or pathological findings. Two PCR-based diagnostic systems for identification of...

journal_title：Journal of clinical microbiology

authors： Persson A,Pettersson B,Bölske G,Johansson KE

更新日期：1999-12-01 00:00:00

abstract：:The PathoTec Rapid I-D System for identifying Enterobacteriaceae was evaluated with 471 cultures. In 4,910 individual test comparisons, 95.5% of the results agreed, with results of only two test strips, those for esculin hydrolysis and urease production, agreeing with conventional tests in less than 94% of the trials....

journal_title：Journal of clinical microbiology

authors： Smith PB,Rhoden DL,Tomfohrde KM

更新日期：1975-04-01 00:00:00

abstract：:CHROMagar Candida is a selective and differential chromogenic medium that has been shown to be useful for identification of Candida albicans, Candida krusei, Candida tropicalis, and perhaps Candida glabrata. Colony morphology and color have been well defined when CHROMagar Candida has been used to isolate yeast direct...

journal_title：Journal of clinical microbiology

authors： Horvath LL,Hospenthal DR,Murray CK,Dooley DP

更新日期：2003-06-01 00:00:00

abstract：:A protocol was optimized for the isolation of Mycobacterium avium subsp. paratuberculosis (MAP) from milk and colostrum, with parameters including chemical decontamination, antibiotics, and different culture media. This study demonstrates that the efficiency of MAP recovery from milk is highly dependent upon the cultu...

journal_title：Journal of clinical microbiology

authors： Bradner L,Robbe-Austerman S,Beitz DC,Stabel JR

更新日期：2013-05-01 00:00:00

abstract：:The first FDA-approved multiplex PCR panel for a large number of respiratory pathogens was introduced in 2008. Since then, other PCR panels for detection of several respiratory and gastrointestinal pathogens have been approved by the FDA and are commercially available, and more such panels are likely to become availab...

journal_title：Journal of clinical microbiology

authors： Schreckenberger PC,McAdam AJ

更新日期：2015-10-01 00:00:00

abstract：:In this study, 5-iodo-2'-deoxyuridine-, cytochalasin B-, and cycloheximide-treated and untreated McCoy cells were inoculated with representative strains of Chlamydia trachomatis serotypes, as well as with clinical specimens from a pediatric population, and the quality and quantity of the cytoplasmic inclusions were ex...

journal_title：Journal of clinical microbiology

authors： La Scolea LJ Jr,Keddell JE

更新日期：1981-04-01 00:00:00

abstract：:Fifty-three isolates of Streptococcus uberis from various sources were examined for the presence of a common antigen. Initially, a serum was produced in rabbits which, by using rocket line immunoelectrophoresis, proved to react with identity to all of the S. uberis crude extracts as well as group B and E streptococcal...

journal_title：Journal of clinical microbiology

authors： Jones KF,Norcross NL

更新日期：1983-05-01 00:00:00

abstract：:Studies on the molecular epidemiologic characteristics of methicillin-resistant Staphylococcus aureus (MRSA) strains have demonstrated their genetic and geographical diversity. In addition, it has been reported that there are genetic differences between community-associated (CA) and health care-associated (HA) MRSA st...

journal_title：Journal of clinical microbiology

authors： Park C,Lee DG,Kim SW,Choi SM,Park SH,Chun HS,Choi JH,Yoo JH,Shin WS,Kang JH,Kim JH,Lee SY,Kim SM,Pyun BY

更新日期：2007-12-01 00:00:00

abstract：:Sepsis is a major cause of death worldwide among nonhospitalized people and hospitalized patients. A wide range of pathogens are involved, and the correct identification and correct antimicrobial therapy are critical to ensure optimal clinical outcomes. With the recent introduction of matrix-assisted laser desorption ...

journal_title：Journal of clinical microbiology

authors： Foster AG

更新日期：2013-11-01 00:00:00

abstract：:A novel PCR restriction analysis method using the RNA polymerase beta-subunit- coding gene (rpoB) was employed to both detect and identify Helicobacter pylori in biopsy specimens and culture isolates. The rpoB DNAs (458 bp) were specifically amplified by PCR with the Helicobacter-specific primers (HF and HR). Based on...

journal_title：Journal of clinical microbiology

authors： Lim CY,Lee KH,Cho MJ,Chang MW,Kim SY,Myong NH,Lee WK,Rhee KH,Kook YH

更新日期：2003-07-01 00:00:00

abstract：:Of 12,321 stool samples analyzed over a 6-year interval, 870 (7.1%) were positive for a total of 1,019 parasites, of which 1,011 (99.2%) were found in trichrome-stained smears of unconcentrated specimens while only 479 (47.0%) were detected in iodine-stained smears of concentrated samples. Stool specimens were next an...

journal_title：Journal of clinical microbiology

authors： Kellogg JA,Elder CJ

更新日期：1999-03-01 00:00:00

abstract：:The region surrounding mecA in methicillin-resistant Staphylococcus aureus (MRSA) is highly variable. We describe an approach for the rapid genotyping of MRSA by assaying for the presence or absence of variable or mobile elements previously shown to be associated with the mecA region. ...

journal_title：Journal of clinical microbiology

authors： Huygens F,Nimmo GR,Schooneveldt J,Munckhof WJ,Giffard PM

更新日期：2002-08-01 00:00:00

abstract：:Although antiretroviral treatment availability has improved, the virological monitoring of patients remains largely uneven across regions. In addition, viral quantification tests are suffering from human immunodeficiency virus type 1 (HIV-1) genetic diversity, fueled by the emergence of new recombinants and of lentivi...

journal_title：Journal of clinical microbiology

authors： Etienne L,Eymard-Duvernay S,Aghokeng A,Butel C,Monleau M,Peeters M

更新日期：2013-03-01 00:00:00

abstract：:From August 1996 to May 1997, six verotoxin-producing Escherichia coli (VTEC) strains were isolated from stool specimens of adults suffering from hemolytic-uremic syndrome (HUS). All the isolates were stx2 positive and belonged to different serotypes: O6:H4, O91:H10, O91:H21, O rough:H16, OX3:H-, and O nontypeable: H-...

journal_title：Journal of clinical microbiology

authors： Bonnet R,Souweine B,Gauthier G,Rich C,Livrelli V,Sirot J,Joly B,Forestier C

更新日期：1998-06-01 00:00:00

abstract：:A modified indirect hemagglutination test for the detection of treponemal antibodies was developed for use with finger-prick blood. By using paired serum and absorbed finger-prick blood from 58 patients from an area previously endemic for yaws and 12 patients without yaws, the modified hemagglutination test was compar...

journal_title：Journal of clinical microbiology

authors： Backhouse JL,Lee MH,Nesteroff SI,Hudson BJ,Hamilton PA

更新日期：1992-03-01 00:00:00

abstract：:A total of 4,409 stool specimens from infants less than 5 years of age seeking treatment for diarrhea in Matlab, Bangladesh, were tested for the presence of adenoviruses by using an enzyme immunoassay (EIA). EIA-positive stool samples were serotyped with monoclonal antibodies specific for adenovirus type 40 (Ad40) and...

journal_title：Journal of clinical microbiology

authors： Jarecki-Khan K,Tzipori SR,Unicomb LE

更新日期：1993-03-01 00:00:00

abstract：:On occasion, a patient may have two or more clinical cultures yielding a coagulase-negative staphylococcus If these multiple isolates have the same phenotype, one might conclude that the same strain was reisolated from the patient, indicating its persistent and pathological presence. We examined the validity of this c...

journal_title：Journal of clinical microbiology

authors： Christensen GD,Parisi JT,Bisno AL,Simpson WA,Beachey EH

更新日期：1983-08-01 00:00:00

abstract：:We adapted a nested Aspergillus PCR to a nested qualitative real-time format on a Light Cycler system. An evaluation using 134 clinical specimens showed that the real-time PCR assay significantly reduced the time for results to be made available without compromising sensitivity and was less labor-intensive. Its use wi...

journal_title：Journal of clinical microbiology

authors： Halliday C,Wu QX,James G,Sorrell T

更新日期：2005-10-01 00:00:00

abstract：:In a multi-health center study, a new rapid optical immunoassay (OIA) for the detection of Shiga toxin types 1 and 2, the BioStar OIA SHIGATOX kit (Inverness Medical Professional Diagnostics, Inc.), was used to prospectively screen 742 fresh fecal samples for Shiga toxins in parallel with the Premier enterohemorrhagic...

journal_title：Journal of clinical microbiology

authors： Teel LD,Daly JA,Jerris RC,Maul D,Svanas G,O'Brien AD,Park CH

更新日期：2007-10-01 00:00:00

abstract：:By using sequence analysis of Shiga toxin 1 (Stx 1) genes from human and ovine Stx-producing Escherichia coli (STEC) strains, we identified an Stx1 variant in STEC of human origin that was identical to the Stx1 variant from ovine STEC, but demonstrated only 97.1 and 96.6% amino acid sequence identity in its A and B su...

journal_title：Journal of clinical microbiology

authors： Zhang W,Bielaszewska M,Kuczius T,Karch H

更新日期：2002-04-01 00:00:00

abstract：:The recovery of Mycobacterium tuberculosis from sputa positive or negative for acid-fast bacilli that were stored for 17 +/- 7 days and inoculated in the BACTEC MGIT 960 system (MGIT) was higher than that from sputa inoculated in Lowenstein-Jensen medium. MGIT is useful for isolation of M. tuberculosis from sputa subj...

journal_title：Journal of clinical microbiology

authors： Pardini M,Varaine F,Bonnet M,Orefici G,Oggioni MR,LONG-DRUG Study Group.,Fattorini L

更新日期：2007-02-01 00:00:00

abstract：:Human metapneumovirus (hMPV) is a recently discovered paramyxovirus that is known to cause respiratory tract infections in children and immunocompromised individuals. Given the difficulties of identifying hMPV by conventional culture, molecular techniques could improve the detection of this virus in clinical specimens...

journal_title：Journal of clinical microbiology

authors： Dare R,Sanghavi S,Bullotta A,Keightley MC,George KS,Wadowsky RM,Paterson DL,McCurry KR,Reinhart TA,Husain S,Rinaldo CR

更新日期：2007-02-01 00:00:00

abstract：:This study assessed the diagnostic performance of the new COVID19SEROSpeed IgM/IgG rapid test (BioSpeedia, a spinoff of the Pasteur Institute of Paris) for the detection of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in comparison to other commercial antibody assays through a large ...

journal_title：Journal of clinical microbiology

authors： Plebani M,Parčina M,Bechri I,Zehender G,Terkeš V,Abdel Hafith B,Antinori S,Pillet S,Gonzalo S,Hoerauf A,Lai A,Morović M,Bourlet T,Torre A,Pozzetto B,Galli M

更新日期：2021-01-21 00:00:00

abstract：:A rapid and accurate method to identify the species and antibiotic resistance of Candida spp. in blood is vital to increase the survival rates of patients with bloodstream infections. However, the extremely low levels of Candida spp. in blood make rapid diagnosis by standard blood culture difficult. In this study, we ...

journal_title：Journal of clinical microbiology

authors： Chen XL,Zheng H,Li WG,Zhong YH,Chen XP,Lu JX

更新日期：2020-03-25 00:00:00

abstract：:Chikungunya fever is a mosquito-borne disease of key public health importance in tropical and subtropical countries. Although severe joint pain is the most distinguishing feature of chikungunya fever, diagnosis remains difficult because the symptoms of chikungunya fever are shared by many pathogens, including dengue f...

journal_title：Journal of clinical microbiology

authors： Okabayashi T,Sasaki T,Masrinoul P,Chantawat N,Yoksan S,Nitatpattana N,Chusri S,Morales Vargas RE,Grandadam M,Brey PT,Soegijanto S,Mulyantno KC,Churrotin S,Kotaki T,Faye O,Faye O,Sow A,Sall AA,Puiprom O,Chaichana P,

更新日期：2015-02-01 00:00:00

abstract：:Pulsed-field gel electrophoresis (PFGE) of XbaI-digested chromosomal DNA was performed on 133 strains of Salmonella enterica serovar Typhi obtained from Papua New Guinea, with the objective of assessing the temporal variation of these strains. Fifty-two strains that were isolated in 1992 and 1994 were of one phage typ...

journal_title：Journal of clinical microbiology

authors： Thong KL,Goh YL,Yasin RM,Lau MG,Passey M,Winston G,Yoannes M,Pang T,Reeder JC

更新日期：2002-11-01 00:00:00

abstract：:Sera from patients with allergic bronchopulmonary aspergillosis (ABPA) or aspergilloma and normal sera were analyzed for specific antibodies by Western (immuno-) blotting with Aspergillus fumigatus antigens transferred electrophoretically onto polyvinylidene difluoride membranes. Western blot analysis demonstrated con...

journal_title：Journal of clinical microbiology

authors： Kurup VP,Greenberger PA,Fink JN

更新日期：1989-06-01 00:00:00

abstract：:A case of non-catheter-related bacteremia caused by Chryseobacterium indologenes in a nonneutropenic man with a solid tumor is described. The patient was successfully treated with piperacillin-tazobactam. ...

journal_title：Journal of clinical microbiology

authors： Christakis GB,Perlorentzou SP,Chalkiopoulou I,Athanasiou A,Legakis NJ

更新日期：2005-04-01 00:00:00

abstract：:In a field trial conducted in cooperation with pediatricians in private practice, 198 nasopharyngeal swabs from children with suspected whooping cough were placed into charcoal-horse-blood transport medium with cephalexin (40 mg/liter). After preincubation at 36 degrees C for 1 to 2 days, the transport systems were ma...

journal_title：Journal of clinical microbiology

authors： Hoppe JE,Weiss A,Wörz S

更新日期：1988-06-01 00:00:00

abstract：:The genetic relatedness of Streptococcus milleri group isolates from the airways of cystic fibrosis patients was determined by using pulsed-field gel electrophoresis. This study reveals no evidence for patient-to-patient transmission in our patient population; however, within individual patients, complex inter- and in...

journal_title：Journal of clinical microbiology

authors： Sibley CD,Sibley KA,Leong TA,Grinwis ME,Parkins MD,Rabin HR,Surette MG

更新日期：2010-07-01 00:00:00