Variable assimilation of carbon compounds by Candida albicans.

abstract：:In a multicenter study, 113 blood samples from 19 organ transplant patients were analyzed for cytomegalovirus by the pp65 antigenemia assay and a quantitative DNA hybridization assay. Overall, there was 84% agreement among the results obtained by the two tests. Fifteen of 16 episodes of active infection were detected ...

journal_title：Journal of clinical microbiology

authors： Bossart W,Bienz K,Wunderli W

更新日期：1997-12-01 00:00:00

abstract：:Vaginal discharge specimens from 105 women were examined by wet mount, culture, and a new direct immunofluorescence assay to detect Trichomonas vaginalis. The organism was detected by culture in 31 patients, by direct immunofluorescence in 26 patients, and by wet mount in 21 patients. ...

journal_title：Journal of clinical microbiology

authors： Smith RF

更新日期：1986-12-01 00:00:00

abstract：:Cellulosimicrobium funkei is a rare, opportunistic pathogen. We describe a case of bacteremia and possibly prosthetic valve endocarditis by this organism in a nonimmunocompromised patient. Useful phenotypic tests for differentiating C. funkei from Cellulosimicrobium cellulans and Cellulosimicrobium terreum include mot...

journal_title：Journal of clinical microbiology

authors： Petkar H,Li A,Bunce N,Duffy K,Malnick H,Shah JJ

更新日期：2011-03-01 00:00:00

abstract：:A case of zygomycosis caused by Apophysomyces elegans in a patient having 25% full-thickness burns is described. Amputation of the leg was necessary to control rapid tissue invasion. The fungus was isolated from soil in the burn environment. ...

journal_title：Journal of clinical microbiology

authors： Cooter RD,Lim IS,Ellis DH,Leitch IO

更新日期：1990-09-01 00:00:00

abstract：:Bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) are orbiviruses that infect both livestock and wild ruminants. Antigenic cross-reactivity between BTV and EHDV often results in serologic misdiagnosis. Competitive enzyme-linked immunosorbent assays (c-ELISAs) show increased sensitivity and specific...

journal_title：Journal of clinical microbiology

authors： Mecham JO,Wilson WC

更新日期：2004-02-01 00:00:00

abstract：:In clinical laboratories, diagnosis of imported malaria is commonly performed by microscopy. However, the volume of specimens is generally low and maintaining proficiency in reading blood smears, particularly at the species level, is challenging in this setting. To address this problem, the Provincial Laboratory for P...

journal_title：Journal of clinical microbiology

authors： Shokoples S,Mukhi SN,Scott AN,Yanow SK

更新日期：2013-06-01 00:00:00

abstract：:Serotyping Streptococcus pneumoniae is a technique generally confined to reference laboratories, as purchasing pneumococcal antisera is a huge investment. Many attempts have been made to modify serological agglutination techniques to make them more accessible, and more recently developments in serotyping have focused ...

journal_title：Journal of clinical microbiology

authors： Batt SL,Charalambous BM,McHugh TD,Martin S,Gillespie SH

更新日期：2005-06-01 00:00:00

abstract：:Three molecular assays were evaluated for the direct detection of Mycobacterium tuberculosis complex bacteria in 125 respiratory and 22 nonrespiratory samples. The overall sensitivities obtained were as follows: GenoType MTBDRplus, 97.9%; GenoType Mycobacteria Direct, 93.7%; Gen-Probe Mycobacterium tuberculosis Amplif...

journal_title：Journal of clinical microbiology

authors： Neonakis IK,Gitti Z,Baritaki S,Petinaki E,Baritaki M,Spandidos DA

更新日期：2009-08-01 00:00:00

abstract：:Accurate and rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) is needed to screen MRSA carriers and improve treatment. The current widely used duplex PCR methods are not able to differentiate MRSA from coexisting methicillin-susceptible S. aureus (MSSA) or other methicillin-resistant staphylo...

journal_title：Journal of clinical microbiology

authors： Luo J,Li J,Yang H,Yu J,Wei H

更新日期：2017-10-01 00:00:00

abstract：:A 42-plex clustered regularly interspaced short palindromic repeat (CRISPR)-based typing technique (spoligotyping) was recently developed at the French National Reference Center for Legionella. It allows the subtyping of the Legionella pneumophila sequence type 1/Paris pulsotype. In this report, we present the transfe...

journal_title：Journal of clinical microbiology

authors： Gomgnimbou MK,Ginevra C,Peron-Cane C,Versapuech M,Refrégier G,Jacotin N,Sola C,Jarraud S

更新日期：2014-07-01 00:00:00

abstract：:A single multiplex PCR assay targeting seven virulence factors and the wzi gene specific for the K1 and K2 capsular serotypes of Klebsiella pneumoniae was developed and tested on 65 clinical isolates, which included 45 isolates responsible for community-acquired severe human infections. The assay is useful for the sur...

journal_title：Journal of clinical microbiology

authors： Compain F,Babosan A,Brisse S,Genel N,Audo J,Ailloud F,Kassis-Chikhani N,Arlet G,Decré D

更新日期：2014-12-01 00:00:00

abstract：:A molecular assay based on a rapid DNA extraction protocol and the EnviroAmp Legionella Kits was used to detect Legionella species in bronchoalveolar fluid specimens. All Legionella strains isolated from tap water in hospitals could be detected distinctly. Both sensitivity and specificity were tested. In a prospective...

journal_title：Journal of clinical microbiology

authors： Kessler HH,Reinthaler FF,Pschaid A,Pierer K,Kleinhappl B,Eber E,Marth E

更新日期：1993-12-01 00:00:00

abstract：:The genetic compositions of 71 isoniazid-resistant Mycobacterium tuberculosis strains from Poland were determined by spoligotyping. Nearly 80% of the isolates belonged to either the T or the Haarlem family. The genotypic diversity was largely due to variation within those families. The scarcity of imported genotypes s...

journal_title：Journal of clinical microbiology

authors： Augustynowicz-Kopec E,Jagielski T,Zwolska Z

更新日期：2008-12-01 00:00:00

abstract：:Testing for susceptibility to pyrazinamide (PZA) and analysis of the pncA gene sequences of 423 Mycobacterium tuberculosis complex isolates have revealed a unique silent nucleotide substitution that enables the rapid identification of "M. canettii" (proposed name). Moreover, the lack of a defined mutation within the p...

journal_title：Journal of clinical microbiology

authors： Somoskovi A,Dormandy J,Parsons LM,Kaswa M,Goh KS,Rastogi N,Salfinger M

更新日期：2007-02-01 00:00:00

abstract：:Coagulase-negative staphylococci obtained from patients with continuous ambulatory peritoneal dialysis-related peritonitis were cultured in nutrient broth in an atmosphere of air containing 5% carbon dioxide (reflecting the CO2 tension found in freshly used dialysate). Significant differences were observed between the...

journal_title：Journal of clinical microbiology

authors： Denyer SP,Davies MC,Evans JA,Finch RG,Smith DG,Wilcox MH,Williams P

更新日期：1990-08-01 00:00:00

abstract：:Gemella morbillorum and Gemella haemolysans are opportunistic pathogens which cause endocarditis and other severe infections. We report on three patients with endocarditis, one with endocarditis caused by G. haemolysans and two with endocarditis caused by G. morbillorum. The paucity of reports concerning these bacteri...

journal_title：Journal of clinical microbiology

authors： La Scola B,Raoult D

更新日期：1998-04-01 00:00:00

abstract：:Hepatitis C virus (HCV) infection typically leads to antibody response within weeks after primary infection. Here, we describe the case of a child infected with HCV by mother-to-child transmission who remained persistently seronegative despite the presence of high levels of circulating HCV RNA. ...

journal_title：Journal of clinical microbiology

authors： Larouche A,Gaëtan G,El-Bilali N,Quesnel-Vallières M,Martin SR,Alvarez F,Shoukry NH,Soudeyns H

更新日期：2012-07-01 00:00:00

abstract：:The performance of a human herpesvirus 8 (HHV-8) enzyme immunoassay (EIA) and selective subsequent use of an HHV-8 immunofluorescence assay (IFA) was tested in persons unlikely to be infected with HHV-8 and those who had HHV-8 detected in their saliva. The IFA was performed on samples within a range of EIA optical den...

journal_title：Journal of clinical microbiology

authors： Casper C,Krantz E,Taylor H,Dalessio J,Carrell D,Wald A,Corey L,Ashley R

更新日期：2002-10-01 00:00:00

abstract：:Mycobacterium microti is a member of the Mycobacterium tuberculosis complex of bacteria. This species was originally identified as a pathogen of small rodents and shrews and was associated with limited diversity and a much reduced spoligotype pattern. More recently, specific deletions of chromosomal DNA have been show...

journal_title：Journal of clinical microbiology

authors： Smith NH,Crawshaw T,Parry J,Birtles RJ

更新日期：2009-08-01 00:00:00

abstract：:About 2.5 years after the first isolation of the VanA phenotype of vancomycin-resistant Enterococcus faecium (VREM) in Poland, the first VREM strains with the VanB phenotype have emerged independently in two different Warsaw hospitals. In one of these the VREM strain was selected during the long-term antimicrobial tre...

journal_title：Journal of clinical microbiology

authors： Kawalec M,Gniadkowski M,Zielińska U,Kłos W,Hryniewicz W

更新日期：2001-02-01 00:00:00

abstract：:Humoral immune response to intestinal Rhodococcus (Corynebacterium) equi in horses was studied by enzyme-linked immunosorbent assay. Anti-R. equi immunoglobulin M (IgM), IgG, and IgA antibodies were demonstrated in the healthy horse population. Adult horse levels of anti-R. equi IgM and IgG antibodies were reached by ...

journal_title：Journal of clinical microbiology

authors： Takai S,Kawazu S,Tsubaki S

更新日期：1986-05-01 00:00:00

abstract：:At the Islet Isolation Laboratory of the Scottish National Blood Transfusion Service, manual sterility testing data show that contamination rates are 57.7% for pancreas transport fluid, 4.3% for postpurification islet samples, and 0% for pretransplant islet samples. This pilot study presents the BacT/Alert System as a...

journal_title：Journal of clinical microbiology

authors： Murray L,McGowan N,Fleming J,Bailey L

更新日期：2014-10-01 00:00:00

abstract：:Whole-genome sequencing (WGS) is now routinely performed in clinical microbiology laboratories to assess isolate relatedness. With appropriately developed analytics, the same data can be used for prediction of antimicrobial susceptibility. We assessed WGS data for identification using open-source tools and antibiotic ...

journal_title：Journal of clinical microbiology

authors： Ferreira I,Beisken S,Lueftinger L,Weinmaier T,Klein M,Bacher J,Patel R,von Haeseler A,Posch AE

更新日期：2020-06-24 00:00:00

abstract：:Multiple-locus variable-number tandem-repeat analysis (MLVA) was performed with 292 methicillin-resistant Staphylococcus aureus (MRSA) isolates previously characterized by pulsed-field gel electrophoresis, multilocus sequence typing, and staphylococcal cassette chromosome mec typing. Quantitative correspondence analys...

journal_title：Journal of clinical microbiology

authors： Rivero-Pérez B,Pérez-Roth E,Méndez-Alvarez S

更新日期：2010-08-01 00:00:00

abstract：:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of an ongoing pandemic that has infected over 36 million and killed over 1 million people. Informed implementation of government public health policies depends on accurate data on SARS-CoV-2 immunity at a population scale. We hypothesized that d...

journal_title：Journal of clinical microbiology

authors： Pisanic N,Randad PR,Kruczynski K,Manabe YC,Thomas DL,Pekosz A,Klein SL,Betenbaugh MJ,Clarke WA,Laeyendecker O,Caturegli PP,Larman HB,Detrick B,Fairley JK,Sherman AC,Rouphael N,Edupuganti S,Granger DA,Granger SW,Coll

更新日期：2020-12-17 00:00:00

abstract：:A colorimeter capable of measuring results of enzyme-linked immunosorbent assay (ELISA) reactions directly in the wells of a microtiter plate is described. This colorimeter proved to be as accurate as a conventional spectrophotometer in assessing ELISA reactions, but had the advantage of not requiring transfer of the ...

journal_title：Journal of clinical microbiology

authors： Clem TR,Yolken RH

更新日期：1978-01-01 00:00:00

abstract：:Chlamydia pecorum is a significant pathogen of domestic livestock and wildlife. We have developed a C. pecorum-specific multilocus sequence analysis (MLSA) scheme to examine the genetic diversity of and relationships between Australian sheep, cattle, and koala isolates. An MLSA of seven concatenated housekeeping gene ...

journal_title：Journal of clinical microbiology

authors： Jelocnik M,Frentiu FD,Timms P,Polkinghorne A

更新日期：2013-08-01 00:00:00

abstract：:The cobas human papillomavirus (HPV) test, approved by the FDA in April 2011, is a fully automated assay for the detection of 14 high-risk (hr) HPV genotypes from cervical specimens collected in liquid-based cytology medium using real-time PCR amplification of the L1 gene and TaqMan probes. Results are simultaneously ...

journal_title：Journal of clinical microbiology

authors： Rao A,Young S,Erlich H,Boyle S,Krevolin M,Sun R,Apple R,Behrens C

更新日期：2013-05-01 00:00:00

abstract：:Rapidly growing mycobacteria are rarely found in central nervous system infections. We describe a case of polymicrobial infection in a brain abscess including two rapidly growing Mycobacterium species, M. immunogenum and M. llatzerense. The Mycobacterium isolates were distinguishable by molecular methods, and whole-ge...

journal_title：Journal of clinical microbiology

authors： Greninger AL,Langelier C,Cunningham G,Keh C,Melgar M,Chiu CY,Miller S

更新日期：2015-07-01 00:00:00

abstract：:Bacterial vaginosis (BV) remains the most common cause of abnormal vaginal discharge in women of reproductive age and is associated with increased susceptibility to human immunodeficiency virus and sexually transmitted infections and preterm delivery. Present diagnostic methods require access to microscopy and laborat...

journal_title：Journal of clinical microbiology

authors： Bradshaw CS,Morton AN,Garland SM,Horvath LB,Kuzevska I,Fairley CK

更新日期：2005-03-01 00:00:00